The superiority and feasibility of 2,3,5-triphenyltetrazolium chloride-stained brain tissues for molecular biology experiments based on microglial properties

作     者：Yajuan Wang Qingrong Zhang Shuchi Zhang Jiangtao Qi Li Li Yajuan Wang;Qingrong Zhang;Shuchi Zhang;Jiangtao Qi;Li Li

作者机构：Department of AnesthesiologyHuashan HospitalFudan UniversityShanghaiChina Department of AnesthesiologyTongji HospitalTongji UniversityShanghaiChina 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2023年第6卷第2期

页      面：111-119页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：National Natural Science Foundation of China,Grant/Award Number:81730031 to lili and 82001395 to yingwei wang the Foundation of Shanghai Municipal Key Clinical Specialty,Grant/Award Number:shslczdzk06901 to yingwei wang 

主　　题：ischemic brain injury microglia transient middle cerebral artery occlusion TTC staining 

摘      要：Background:TTC(2,3,5-triphenyltetrazolium chloride)staining is the most commonly used method in identifying and assessing cerebral infarct volumes in the transient middle cerebral artery occlusion *** that microglia exhibit different morphologies in different regions after ischemic stroke,we demonstrate the superiority and necessity of using TTC-stained brain tissue to analyze the expression of various proteins or genes in different regions based on microglia ***:We compared brain tissue(left for 10 min on ice)from the improved TTC staining method with penumbra from the traditional sampling *** identified the feasibility and necessity of the improved staining method using real time(RT)-PCR,Western blot,and immunofluorescence ***:There was no protein and RNA degradation in the TTC-stained brain tissue ***,the TREM2 specifically expressed on the microglia showed a significant difference between two groups in the penumbra ***:TTC-stained brain tissue can be used for molecular biology experiments without any *** addition,TTC-stained brain tissue shows greater superiority due to its precise positioning.