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N6-腺苷酸甲基化修饰在呼吸系统疾病发生发展中作用的研究进展

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中华危重病急救医学 2024年第3期36卷 303-307页

作者：李海博杨静帆秦燕勤李建生河南中医药大学河南省中医药防治呼吸病重点实验室呼吸疾病中医药防治省部共建协同创新中心郑州450046

N6-腺苷酸甲基化(m6A)是真核生物mRNA最常见的转录后修饰行为之一。m6A修饰可加速mRNA的代谢和翻译,在细胞分化、胚胎发育和免疫应答等过程中发挥重要作用。作为一种可逆的表观遗传修饰,m6A修饰在许多生理和病理过程中发挥着重要作用。... 详细信息

N6-腺苷酸甲基化(m6A)是真核生物mRNA最常见的转录后修饰行为之一。m6A修饰可加速mRNA的代谢和翻译,在细胞分化、胚胎发育和免疫应答等过程中发挥重要作用。作为一种可逆的表观遗传修饰,m6A修饰在许多生理和病理过程中发挥着重要作用。m6A修饰与呼吸系统疾病的发生发展密切相关,m6A修饰调控因子可能成为调控呼吸系统疾病的潜在作用靶点。本文对m6A修饰在肺癌、急性肺损伤(ALI)、哮喘、肺纤维化、慢性阻塞性肺疾病(COPD)等呼吸系统疾病发生发展中的作用进行了综述,旨在为呼吸系统疾病的发病机制及药物作用靶点研究提供参考。

关键词： N6-腺苷酸甲基化修饰 RNA甲基化呼吸系统疾病研究进展

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清醒俯卧位在急性低氧性呼吸衰竭中的应用

中华危重病急救医学 2024年第3期36卷 331-331页

作者：蒋佳维(编译) 陆军军医大学大坪医院战伤感染与特需药品研究室，创伤与化学中毒全国重点实验室，重庆 400042 中国人民解放军联勤保障部队第九二六医院，云南开远 661600

在患有中重度急性呼吸窘迫综合征(ARDS)的插管患者中,俯卧位可减少肺内分流并产生肺复张,优化通气/血流比值,减少肺泡死腔并降低右心室后负荷,从而提高中重度ARDS插管患者的生存率。但很少有研究探讨俯卧位对有自主呼吸且存在急性低氧... 详细信息

在患有中重度急性呼吸窘迫综合征(ARDS)的插管患者中,俯卧位可减少肺内分流并产生肺复张,优化通气/血流比值,减少肺泡死腔并降低右心室后负荷,从而提高中重度ARDS插管患者的生存率。但很少有研究探讨俯卧位对有自主呼吸且存在急性低氧性呼吸衰竭患者的生理影响。近期有意大利学者进行了一项序贯、交叉试验,旨在全面评估清醒俯卧位对急性低氧性呼吸衰竭患者生理反应(包括气体交换、呼吸努力、肺容量和充气模式)的影响。研究共纳入了15例急性低氧性呼吸衰竭且氧合指数(PaO2/FiO2)<200 mmHg(1 mmHg≈0.133 kPa)患者,接受经鼻高流量氧疗仰卧位1 h、俯卧位2 h、仰卧1 h。在每个阶段结束时,测量动脉血气分析、吸气努力(ΔPES)、跨肺驱动压(ΔPL)、呼吸频率、简化食道压-时间乘积(sPTPES)、潮气量(VT)、呼气末肺阻抗(EELI)、肺顺应性、气道阻力、时间常数、动态应变(VT/EELI)及使用电阻抗断层扫描的Pendelluft程度。

关键词：动脉血气分析气道阻力俯卧位肺顺应性肺容量仰卧位驱动压交叉试验

Tiaobu Feishen therapy inhibits inflammation induced by cigarette smoke extracts in a human monocyte/macrophage cell line

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Journal of Traditional Chinese Medicine 2021年第3期41卷 360-366页

作者： qin yanqin CHEN Yulong ZHAO Peng FENG Suxiang WU Yaosong LIU Xuefang DONG Haoran ZHENG Wanchun MAO Xiaoning LI Jiansheng Henan Key Laboratory of Chinese Medicine for Respiratory Disease Henan University of Chinese MedicineZhengzhou 450046China Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of China Henan University of Chinese MedicineZhengzhou 450046China

OBJECTIVE: To study the mechanistic effects of Tiaobu Feishen therapy(TBFS) on inflammation induced by cigarette smoke extract(CSE) in a human monocyte/macrophage cell line.METHODS: The human monocyte/macrophage cell line THP-1 was stimulated with 10% CSE in the presence or absence of Bufei Yishen formula(BYF),Bufei Jianpi formula(BJF) and Yiqi Zishen formula(YZF). All formulations contained serum. Pro-inflammatory cytokines were measured in the supernatants using enzyme-linked immunosorbent assay.The activity of STAT3 DNA binding was detected using electrophoretic mobility shift assay and janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway activation was assessed using Western blotting.RESULTS: The results showed that BYF, BJF and YZF treatment strongly decreased the CSE-induced secretion of interleukin(IL)-6, IL-8, tumor necrosis factor-α and matrix metalloproteinase-9 by THP-1 cells. Furthermore, BYF, BJF and YZF treatment attenuated STAT3 DNA binding capacity and JAK2 and STAT3 were shown to be phosphorylated.CONCLUSION: The data revealed that BYF, BJF and YZF effectively inhibited a CSE-induced inflammatory response in THP-1 cells by limiting activation of the JAK2/STAT3 pathway.

关键词： cigarette smoking janus kinase 2 STAT3 transcription factor THP-1 cells matrix metalloproteinases Tiaobu Feishen therapy

Three Tiaobu Feishen formulae reduces cigarette smoke-induced inflammation in human airway epithelial cells

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Journal of Traditional Chinese Medicine 2020年第3期40卷 386-392页

作者： Dong Haoran Liu Xuefang Zheng Wanchun Feng Suxiang Li Jiansheng qin yanqin Wu Yaosong Chen Yulong Yin Sugai Zhao Peng Henan Key Laboratory of Chinese Medicine for Respiratory Disease Henan University of Chinese MedicineZhengzhou 450046China Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment&Chinese Medicine Development of Henan Province Henan University of Chinese MedicineZhengzhou 450046China

OBJECTIVE: To investigate the therapeutic efficacy of Tiaobu Feishen formulae(TBFS) on cigarette smoke-induced inflammation in vitro using lipopolysaccharide(LPS)-induced and cigarette smoke extract(CSE)-induced NCI-H292 cells.METHODS: We evaluated the inhibitory effects of Bufei Jianpi formula(BJF), Bufei Yishen formula(BYF), and Yiqi Zishen formula(YZF) on the expressions of inflammatory cytokines including tumor necrosis factor(TNF)-α and interleukin(IL)-8, matrix metalloproteinase(MMP)-9, tissue inhibitor of matrix metalloprotease(TIMP)-1, and superoxide dismutase(SOD) in H292 cells stimulated with LPS or CSE. Their related transcription factors and signaling pathways were also analyzed.RESULTS: BJF, BYF, and YZF significantly inhibited the LPS-or CSE-induced expressions of TNF-α, IL-8,MMP-9, TIMP-1, and SOD in H292 cells, and suppressed the activation of transcription factors including nuclear transcription factor(NF)-κB, activator protein(AP)-1, and signal transducers and activators of transcription(STAT) 3 and their corresponding pathways, including NF-κB, mitogen-activated protein kinase(MAPK), STAT3, and peroxisome proliferator-activated receptor(PPAR).CONCLUSION: BJF, BYF, and YZF effectively suppressed inflammatory responses, protease-antiprotease imbalance, and oxidative stress induced by LPS and CSE, an effect that was closely associated with the inhibition of the NF-κB, MAPK, STAT3, and PPAR pathways.

关键词： Pulmonary disease chronic obstructive Lipopolysaccharides Cigarette smoking Inflammation Epithelial cells Tiaobu Feishen formulae

Three Tiaobu Feishen therapies protect human alveolar epithelial cells against cigarette smoking and tumor necrosis factor-mlear factor-kappa B pathwayα-induced inflamation by nuc

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Journal of Traditional Chinese Medicine 2019年第1期39卷 45-49页

作者： Chen Yulong Wu Yaosong Li Jiansheng Feng Suxiang Hao Lili Liu Xuefang Zheng Wanchun Dong Haoran qin yanqin Yin Sugai Zhao Peng Henan Key Laboratory of Chinese Medicine for Respiratory Disease Henan University of Chinese MedicineZhengzhou 450046China Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment&Chinese Medicine Development of Henan Province Henan University of Chinese MedicineZhengzhou 450046China

OBJECTIVE: To investigate the efficacy of Tiaobu Feishen formulae(TBFS), including Bufei Jianpi formula(BJF), Bufei Yishen formula(BYF), and Yiqi Zishen formula(YZF), on inflammatory response, protease-anti-protease imbalance and collagen deposition in rats.METHODS: In present work, we used an in vitro model of cigarette smoking extract(CSE)-and tumor necrosis factor-α(TNF-α)-induced A549 cellsto examine the efficacy of BJF, BYF and YZF on the production of inflammatory cytokines, including TNF-α and interleukin(IL)-8, IL-6, matrix metalloproteinases(MMP)-9, and IL-10 in CSE or TNF-ls. And their related transcripα-induced A549 celtion factors and signaling pathway were also analyzed.RESULTS: The results showed that BJF, BYF and YZF could significantly decrease the expression levels of the pro-inflammatory cytokines induced by CSE or TNF-α. Furthermore, BJF, BYF and YZF could suppress CSE-or TNF-α-induced activation of nuclear factor-kappa B(NF-κB) transcription factors and its corresponding pathways. Taken together, these data implied that BJF, BYF and YZF effectively inhibited CSE-or TNF-α-induced inflammatory response in alveolar epithelial cell, which was due to their inhibition effect on NF-κB pathways.CONCLUSION: Our findings suggest that the Tiaobu Feishen therapies may protect human alveolar epithelial cells against cigarette smoking and TNF-α-induced inflammation. NF-κB pathway may involve in the actions.

关键词： Chinese medical formula Cigarette smoking Tumor necrosis factor-alpha A549 cells

中医药调控线粒体损伤干预呼吸系统疾病的研究进展

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Chinese Medicine and Natural Products 2024年第2期4卷 49-55页

作者：李海博杨静帆秦燕勤刘田田 Henan Key Laboratory of Chinese Medicine for Respiratory Disease Henan University of Chinese MedicineCo-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan and Education Ministry of P.R.ChinaZhengzhouHenanChina Academy of Chinese Medical Sciences Henan University of Chinese MedicineZhengzhouHenanChina

Mitochondria are important organelles in cells for energy production,and mitochon-drial damage caused by various reasons is an important factor promoting disease progression.Mitochondrial damage involves structural damage and mitochondrial DNA damage,which are closely related to the occurrence and development of respiratory system diseases.In recent years,a large number of studies have confirmed the significant role of mitochondrial damage in the progression of respiratory system diseases,which may be an important target for the treatment of respiratory system diseases with traditional Chinese medicine(TCM).This article reviews the role of TCM in regulating mitochondrial damage for intervention in respiratory system diseases such as chronic obstructive pulmonary disease,lung cancer,pulmonary fibrosis,acute lung injury,asthma,and pneumonia,aiming to provide a basis for the study of the pathogenesis and drug action targets of respiratory system diseases.

关键词： mitochondrial damage respiratory system diseases TCM

围绝经期女性抑郁发生情况及与家庭亲密度和主观幸福感的关系

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保健医学研究与实践 2024年第1期21卷 29-34页

作者：张艳琴刘晓红韩芸芸王景媛杨琴首都医科大学附属北京世纪坛医院妇科北京100038 中国人民解放军总医院第六医学中心妇产科北京100048

目的调查围绝经期女性抑郁的发生情况,并探究围绝经期抑郁与家庭亲密度和主观幸福感的关系。方法选取2022年1月—2023年6月于我院妇产科就诊的378例围绝经期女性为调查对象。使用患者健康问卷抑郁量表(PHQ-9)、一般资料调查表、家庭亲... 详细信息

目的调查围绝经期女性抑郁的发生情况,并探究围绝经期抑郁与家庭亲密度和主观幸福感的关系。方法选取2022年1月—2023年6月于我院妇产科就诊的378例围绝经期女性为调查对象。使用患者健康问卷抑郁量表(PHQ-9)、一般资料调查表、家庭亲密度和适应性量表中文版(FACESⅡ-CV)亲密度量表、总体幸福感量表(GWB)对调查对象进行问卷调查。利用多因素Logistics回归分析围绝经期女性抑郁的影响因素,使用Pearson相关分析PHQ-9得分与FACESⅡ-CV亲密度量表得分、GWB得分间的关系。结果调查对象抑郁检出率为33.86%(128/378)。不同文化水平、月经情况、改良Kupperman指数、工作状态、婚姻状态的调查对象抑郁检出率的差异均有统计学意义(P<0.05),且抑郁组与正常组调查对象的FACESⅡ-CV亲密度量表得分、GWB得分差异均有统计学意义(P<0.05)。多因素Logistics回归分析结果显示:文化水平、改良Kupperman指数、FACESⅡ-CV亲密度量表得分、GWB得分为围绝经期女性抑郁的独立影响因素(P<0.05)。Pearson相关分析结果显示:PHQ-9得分与FACESⅡ-CV亲密度量表得分、GWB得分均呈负相关(r=-0.500、-0.407,P<0.001)。结论围绝经期女性抑郁的检出率较高,临床需加以重视。抑郁的发生主要受文化水平、围绝经期症状严重程度、家庭亲密度和主观幸福感影响,因此在围绝经期女性的临床治疗及护理实践中应注意围绕上述几方面的具体情况制定针对性措施。

关键词：围绝经期抑郁围绝经期综合征家庭亲密度主观幸福感

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A fluid flow model in the lacunar-canalicular system under the pressure gradient and electrical field driven loads

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Applied Mathematics and Mechanics(English Edition) 2022年第6期43卷 899-916页

作者： Xiaogang WU Xiyu WANG Chaoxin LI Zhaowei WANG Yuqin SUN Yang YAN Yixian qin Pengcui LI yanqin WANG Xiaochun WEI Weiyi CHEN College of Biomedical Engineering Taiyuan University of TechnologyTaiyuan 030024China Shanxi Provincial Key Laboratory for Repair of Bone and Soft Tissue Injury Taiyuan 030001China

The lacunar-canalicular system(LCS)is acknowledged to directly participate in bone tissue remodeling.The fluid flow in the LCS is synergic driven by the pressure gradient and electric field loads due to the electro-mechanical properties of bone.In this paper,an idealized annulus Maxwell fluid flow model in bone canaliculus is established,and the analytical solutions of the fluid velocity,the fluid shear stress,and the fluid flow rate are obtained.The results of the fluid flow under pressure gradient driven(PGD),electric field driven(EFD),and pressure-electricity synergic driven(P-ESD)patterns are compared and discussed.The effects of the diameter of canaliculi and osteocyte processes are evaluated.The results show that the P-ESD pattern can combine the regulatory advantages of single PGD and EFD patterns,and the osteocyte process surface can feel a relatively uniform shear stress distribution.As the bone canalicular inner radius increases,the produced shear stress under the PGD or P-ESD pattern increases slightly but changes little under the EFD pattern.The increase in the viscosity makes the flow slow down but does not affect the fluid shear stress(FSS)on the canalicular inner wall and osteocyte process surface.The increase in the high-valent ions does not affect the flow velocity and the flow rate,but the FSS on the canalicular inner wall and osteocyte process surface increases linearly.In this study,the results show that the shear stress sensed by the osteocyte process under the P-ESD pattern can be regulated by changing the pressure gradient and the intensity of electric field,as well as the parameters of the annulus fluid and the canaliculus size,which is helpful for the osteocyte mechanical responses.The established model provides a basis for the study of the mechanisms of electro-mechanical signals stimulating bone tissue(cells)growth.

关键词： bone canaliculi osteocyte process pressure gradient electricity fluid shear stress(FSS)

综合肺康复方案对急性缺血性脑卒中重度偏瘫患者疲劳及运动功能的影响

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蛇志 2024年第1期36卷 107-111页

作者：陆秋芳应燕萍马娟黄安石覃艳勤广西医科大学第一附属医院神经内科广西南宁530021 广西医科大学第一附属医院护理部广西南宁530021

目的探讨综合肺康复方案对急性期缺血性脑卒中重度偏瘫患者卒中后疲乏、运动功能及肺炎发生率的影响。方法选取2020年6月至2022年6月在某三级综合甲等医院神经内科住院的151例急性期缺血性脑卒中重度偏瘫患者作为研究对象,其中以2020年... 详细信息

目的探讨综合肺康复方案对急性期缺血性脑卒中重度偏瘫患者卒中后疲乏、运动功能及肺炎发生率的影响。方法选取2020年6月至2022年6月在某三级综合甲等医院神经内科住院的151例急性期缺血性脑卒中重度偏瘫患者作为研究对象,其中以2020年6月至2021年6月符合入选标准的78例患者作为对照组,以2021年7月至2022年6月符合入选标准的73例患者作为试验组。对照组实施常规治疗和护理,试验组在常规治疗和护理基础上实施综合肺康复方案,两组患者均在实施干预前后采用疲劳严重程度量表(FSS)进行评定患者的疲乏程度、Fugl-Meyer评定量表(FMA)评价患者运动功能,并比较两组患者的肺炎发生率。结果两组患者的疲劳严重程度评分均较干预前降低,试验组的评分明显低于对照组,差异均有统计学意义(均P<0.05)。两组患者的FMA评分均较治疗前有提高,试验组的得分明显高于对照组,差异均有统计学意义(均P<0.05)。对照组的肺炎发生率为21.79%(17/78),试验组的肺炎发生率为8.22%(6/73),组间比较差异有统计学意义(χ2=5.382,P=0.020)。结论实施综合肺康复方案可降低卒中后疲劳程度,提高患者运动功能,减少肺炎发生率,值得临床应用。

关键词：肺康复缺血性脑卒中偏瘫疲劳运动功能