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Estimation of genetic parameters for upper thermal tolerance and growth-related traits in turbot Scophthalmus maximus using the Bayesian method based on Gibbs sampling

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Acta Oceanologica Sinica 2018年第6期37卷 40-46页

作者： MA Aijun WANG Xin'an HUANG Zhihui LIU Zhifeng CUI Wenxiao qu jiangbo Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology Qingdao 266071 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Yantai Tianyuan Aquatic Limited Corporation Yantai 264003 China

In order to carry out the genetic improvement of turbot upper thermal tolerance, it is necessary to estimate the genetic parameters of UTT（upper thermal tolerance） and growth-related traits. The objective of this study was to estimate genetic parameters for BW（body weight） and UTT in a two-generational turbot（Scophthalmus maximus L.） pedigree derived from four imported turbot stocks（England, France, Denmark and Norway）. A total of 42 families including 20 families from G1 generation and 22 families from G2 generation were used to test upper thermal tolerance（40–50 animals per family） in this study and the body weight of individuals were measured. The heritability of BW and UTT and the correlation between these two traits were estimated based on an individual animal model using Bayesian method based on two types of animal models with and without maternal *** results showed that the heritabilities for BW and UTT and phenotypic and genetic correlations between the two traits estimated from model without maternal effects were 0.239±0.141, 0.111±0.080, 0.075±0.026 and–0.019±0.011, respectively. The corresponding values from model with maternal effects were 0.203±0.115,0.055±0.026, 0.047±0.034 and –0.024±0.028, respectively. The maternal effects of BW and UTT were 0.050±0.017 and 0.013±0.004, respectively. The maternal effects had a certain influence on the genetic evaluation of the two traits. The findings of this paper provided the necessary background to determine the best selection strategy to be adopted in the genetic improvement program.

关键词： heritability genetic correlation thermotolerance body weight Turbot Bayesian inference

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Estimation of genetic parameters for upper thermal tolerances and growth-related traits in turbot Scophthalmus maximus

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Journal of Oceanology and Limnology 2019年第5期37卷 1736-1745页

作者： WANG Xin′an MA Aijun HUANG Zhihui SUN Zhibin CUI Wenxiao qu jiangbo YU Hong Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding Qingdao Key Laboratory for Marine Fish Breeding and BiotechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China Yantai Tianyuan Aquatic Limited Corporation Yantai 264006China

An abnormally high temperature produces a stress response in turbot causing large economic losses in the turbot aquaculture industry of China. A genetic improvement of the upper thermal tolerance (UTT) of turbot could allow cultured fi sh to adapt. A genetic evaluation of UTT is required for determining the practicability of including this trait into a breeding program. In this study, data were recorded from a temperature tolerance test conducted on 3 200 individual turbots from 32 full-sib groups. A cross-sectional linear model and a cross-sectional threshold probit model were used to analyze the test-period survival and a cross-sectional threshold logit model was used to analyze the test-day survival. In addition, phenotypic and genetic correlations between body weight and survival data were estimated. The estimated heritability values obtained from the cross-sectional linear model (CSL), the cross-sectional threshold (probit) model (THRp), and the cross-sectional threshold (logit) model (THRl) were 0.247 9±0.108 3, 0.288 3±0.161 2, and 0.106 9±0.045 2, respectively. The correlation coeffi cients among the full-sib family estimated breeding values (EBVs) obtained from the three models were greater than 0.998 6 and all models produced an almost identical family ranking. The accuracies of selection obtained with the CSL, THRp, and THRl model were 0.773 8, 0.775 4, and 0.784 4, respectively, the greatest from the THRl model. The genetic correlations between body weight and survival data EBVs from the CSL, THRp, and THRl models were 0.020 1,-6.201 1×10^-4 , and -3.115 4×10^-4 , respectively, and the phenotypic correlations between the two traits were -0.837 1 and -0.667 1, respectively. The findings of this study provide background information to determine the best strategy of selection for the genetic improvement of UTT in turbot.

关键词： Scophthalmus maximus upper thermal tolerance genetic parameters cross-sectional models

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Molecular Characterization, Expression Pattern and Transcriptional Regulation of Figla During Gonad Development in Japanese Founder (Paralichthys olivaceus)

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Journal of Ocean University of China 2022年第4期21卷 1037-1050页

作者： qu jiangbo LI Rui LIU Yuxiang SUN Minmin YAN Weijie LIU Jinxiang WANG Xubo ZHANG quanqi MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for MarineScience and TechnologyQingdao 266237China

The factor in the germline alpha(figla), as a member of the basic helix-loop-helix family, has been reported to be involved in ovary development in mammals and teleosts. However, the regulatory mechanisms of figla in teleosts remain unclear. Here,figla in P. olivaceus(Pofigla) was characterized with encoding a 202 amino acid protein that contains a conserved basic region and helix-loop-helix(HLH) domain. Amino acids alignment and synteny analysis revealed that Pofigla was conserved with the orthologous gene sequences in other vertebrates. The results of qRT-PCR showed Pofigla was maternally inherited during embryonic development. For tissue distribution, Pofigla showed a sexually dimorphic gene expression in the gonad of different genders, with a higher expression in ovary than in testis. In situ hybridization(ISH) results demonstrated Pofigla was specifically expressed in germ cells including oocytes, spermatogonia and spermatocytes. By screening and analyzing two proximal regions(-2966/-2126 and-772/-444) with high promoter activity, we found SOX5, LEF1, FOXP1 and GATA1 may play important roles in the transcriptional regulation of Pofigla. Furthermore, we observed the co-localization between Figla and LEF1 in HEK 293T cells. And the significant up-regulation effect of the canonical Wnt signaling pathway on the expression of Pofigla was found in cultured ovarian cells. This study provided the first evidence that figla not only has an important function in ovary development, but also plays some potential roles in testis development and/or male germ cell differentiation during early testis development in P. olivaceus. The results provide valuable reference in exploring the regulatory network of figla in teleost.

关键词： figla transcriptional regulation Wnt signaling pathway gonad development Paralichthys olivaceus

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Hypothalamic-Pituitary-Gonadal(HPG)Axis and Transcriptional Regulatory Elements Regulate piwil2 Gene Expression During Gametogenesis and Gonadal Development in Japanese Flounder(Paralichthys olivaceus)

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Journal of Ocean University of China 2020年第6期19卷 1378-1388页

作者： NI Feifei YU Haiyang qu jiangbo MENG Lihui LIU Xiumei YAN Weijie CHANG Jing ZHANG quanqi WANG Xubo YU Haiyang Key Laboratory of Marine Genetics and Breeding Ministry of EducationOcean University of ChinaQingdao 266003China College of Life Sciences Yantai UniversityYantai 264005China

The P-element induced wimpy testis(Piwi)proteins,which are associated with PIWI-interacting RNAs(piRNAs),play important roles in meiosis,germ cell division,and germline *** this study,we identified and characterized the Paralichthys olivaceus piwil2 gene,a constituent factor of the piRNA pathways involved in the biogenesis of reproductive *** biological analysis indicated that piwil2,which contains PAZ and PIWI domains,was highly conserved between teleosts and *** piwil2 distribution profile in different tissues confirmed a sexually dimorphic expression pattern,with a higher expression level in *** situ hybridization demonstrated that piwil2 was expressed in the oogonia and oocytes of the ovaries as well as in the Sertoli cells and spermatocytes of the *** piwil2 showed a maternally inherited expression pattern during embryonic development,and was highly expressed during the early embryonic *** luciferase reporters were constructed to determine the transcriptional regulatory mechanisms of *** piwil2 core promoter region was located at−360 bp to−60 ***,some representative sex hormones,including human chorionic gonadotropin,17α-methyltestosterone,and estradiol-17βhad distinct regulatory effects on *** a summery,these results indicate that piwil2,regulated by sex hormones and transcriptional elements,has vital functions in the reproductive cycle and gonadal development.

关键词： piwil2 gametogenesis and gonadal development HPG axis transcriptional regulatory elements Paralichthys olivaceus

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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

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Journal of Oceanology and Limnology 2020年第2期38卷 467-479页

作者： CUI Wenxiao MA Aijun HUANG Zhihui WANG Xin’an SUN Zhibin LIU Zhifeng ZHANG Wei YANG Jingkun ZHANG Jinsheng qu jiangbo College of Fisheries and Life Science Shanghai Ocean UniversityMinistry of EducationShanghai 201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShandong Key Laboratory of Marine Fisheries Biotechnology and Genetic BreedingQingdao Key Laboratory for Marine Fish Breeding and BiotechnologyQingdao 266071China Yantai Tianyuan Aquatic Limited Corporation Yantai 264003China

Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory *** kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5)and seawater(salinity 30)was sequenced and *** silico analysis indicated that all unigenes had significant hits in seven *** functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion,absorption,and metabolism played an important role in the osmoregulation of turbot in response to *** of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic ***,we analyzed and predicted the mechanisms of canonical signaling *** processes involved in renin secretion,ECM-receptor interaction,adherens junction,and focal adhesion played an important role in the plasticity phenotype in hypo-stress,while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse,worked in hypoosmoregulation signal transduction in the *** addition,analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried *** results showed that the kidney,gill,and spleen were vital regulating organs of osmotic pressure,and the osmoregulation pattern of euryhaline fish dif fered among species.

关键词： osmoregulation mechanisms Scophthalmus maximus hypo-saline signal transduction cell volume regulation gene expression

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Molecular characterization and expression analysis of Lily- type lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum

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Journal of Oceanology and Limnology 2018年第2期36卷 508-518页

作者： XIA Dandan MA Aijun HUANG Zhihui SHANG Xiaomei CUI Wenxiao YANG Zhi qu jiangbo Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Ocean University of Shanghai Shanghai 201306 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Yantai Tianyuan Aquatic Limited Corporation Yantai 264006 China

A full-length lily-type lectin （SmLTL） was identified from turbot （Scophthalmus maximus） in this study. By searching database for protein identification and fimction prediction, SmLTL were confirmed. The full-length cDNA of SmLTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The SmLTL peptide is characterized by a specific J3-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30-99; two of the repeats share the classical mannose binding domain （QxDxNxVxY） while the third binding site was similar to other fish-specific binding motifs （TxTxGxRxV）. The primary, secondary, and tertiary structures of SmLTL were predicted and analyzed, indicating that the SmLTL protein was hydrophilic, contained 5.36% a-helices, 39.29% extended strands, 16.07% [3-folds, and 39.29% random coils, and three [3-folds. quantitative real- time polymerase chain reaction （qPCR） analysis revealed that the SmLTL mRNA was abundantly expressed in skin, gill, and intestine. Low levels of SmLTL expression were observed in other tissues. The expression of SmLTL in gill, skin and intestine increased at mRNA level after stimulation of Hbrio anguillarum, our results suggest that SmLTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that SmLTL is a member of the lily- type lectin family and the information reported here will provide an important foundation for future research on the role of this protein.

关键词： lily-type lectin full-length of cDNA quantitative real-time polymerase chain reaction protein structure Vibrio anguillarum

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Genome-wide identification and transcriptome-based expression analysis of sox gene family in the Japanese flounder Paralichthys olivaceus

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Journal of Oceanology and Limnology 2018年第5期36卷 1731-1745页

作者： YU Haiyang DU Xinxin LI Xiaojing qu jiangbo ZHU He ZHANG quanqi WANG Xubo Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266000China

Sox genes are transcription factors that ubiquitously exist in animal species, and share a conserved high mobility group(HMG) box. They play important biological roles in diverse developmental processes, such as sex determination and differentiation, chondrogenesis, neurogenesis, and early embryonic development. In this study, we identified 25 sox genes from genome and transcriptome of Japanese flounder Paralichthys olivaceus. These s ox genes could be mainly classified into seven subfamilies(B1, B2, C, D, E, F, and K), and each subfamily exhibited a relatively conserved gene structure. Besides, subfamilies A and G were found exclusively in human and mouse, and sox 32 in subfamily K only existed in teleosts. Compared with other mammals, some s ox genes in teleosts had two duplicates. The loss, duplication, and divergence of sox genes during evolution provided an evidence for whole-genome duplication that occurred in the radiation of teleosts. The expression of Japanese flounder sox genes was also analyzed by FPKM value. Our results showed that certain s ox genes exhibited obviously tissue-specific and spatio-temproal expression. Especially, gonal-basied expression analysis uncovered that s ox7 and s ox2 were ovary-biased, and s ox8 b was testis-biased. Moreover, sox10 a was expressed specifically in ovary, and sox8 a in testis. Therefore this study provide a solid foundation for future functional and evolutionary analysis of sox genes in Japanese flounder.

关键词： sox Japanese flounder gene structure gene expression

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先天性矫正型大动脉转位解剖纠治术与Fontan术后早中期结果的单中心回顾性队列研究

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中国胸心血管外科临床杂志 2023年第9期30卷 1308-1315页

作者：王涛陈欣欣邹明晖曲江波广州医科大学附属广州市妇女儿童医疗中心心脏中心广州510623

目的比较接受解剖纠治术和Fontan术的先天性矫正型大动脉转位(congenitally corrected transposition of the great arteries,ccTGA)患者的早中期预后。方法回顾性分析2009年1月—2021年9月在广州医科大学附属广州市妇女儿童医疗中心接... 详细信息

目的比较接受解剖纠治术和Fontan术的先天性矫正型大动脉转位(congenitally corrected transposition of the great arteries,ccTGA)患者的早中期预后。方法回顾性分析2009年1月—2021年9月在广州医科大学附属广州市妇女儿童医疗中心接受治疗53例ccTGA患者的临床资料,其中男41例、女12例,根治术时年龄55.02(3~168)个月。根据手术方式将患者分为解剖纠治组(16例)和Fontan组(37例),对两组患儿术后生存率、并发症、免于再干预率等进行比较。另招募180名健康儿童作为对照组,采用倾向性评分匹配方法匹配14名儿童进入Fontan对照组。比较Fontan组和Fontan对照组心肺运动试验(cardiopulmonary exercise testing,CPET)结果。结果解剖纠治组早期死亡2例(12.5%),早期再干预3例(18.8%),9例(56.3%)并发心律失常;Fontan组早期死亡1例(2.7%),早期再干预2例,6例(16.2%)并发心律失常。与解剖纠治组相比,Fontan组的体外循环时间、主动脉阻断时间、气管插管时间及住ICU时间缩短,差异有统计学意义(P0.05)。末次随访时48例(100.0%)心功能分级均为Ⅰ/Ⅱ级。结论在ccTGA的外科治疗中,解剖纠治术或Fontan术的无移植生存率无明显差异,但Fontan组的术后并发症发生率相对更低。即使患者满足解剖纠治的条件,Fontan术也可纳入考虑范围。

关键词：先天性矫正型大动脉转位解剖纠治 Fontan术心肺运动试验

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高职教师成长:内涵、机理与策略

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教师教育论坛 2023年第3期36卷 65-71页

作者：曲江波陈庆新聊城职业技术学院山东聊城252000 山东师范大学教育学部济南250014

教师成长的主要观测点是教师专业的成长与发展,是学校和教师共同采取合适措施(制度)不断匹配人才培养需求的过程。在这个过程中,社会规范与个性特征、社会认同与自我认同双向动态调整,无限耦合,直至达成有机统一。高职教师成长是多方面... 详细信息

教师成长的主要观测点是教师专业的成长与发展,是学校和教师共同采取合适措施(制度)不断匹配人才培养需求的过程。在这个过程中,社会规范与个性特征、社会认同与自我认同双向动态调整,无限耦合,直至达成有机统一。高职教师成长是多方面、多因素共同作用的结果,成长过程主要包括新手、高级新手、胜任者、精通者、专家等五个阶段。教育行政部门、学校和教师个人,应共同制定出分类型、分层次、个性化的高职教师成长标准,优化高职教师成长路径,以持续发挥高职教师对新时代职业教育高质量发展的基石作用。

关键词：高职教师专业成长机理多类型

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大菱鲆水通道蛋白(AQP1、AQP3)以及离子通道蛋白(CFTR、NHE1)对低盐胁迫的响应

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渔业科学进展 2020年第4期41卷 41-49页

作者：张金生刘志峰马爱军崔文晓曲江波中国水产科学研究院黄海水产研究所山东省海洋渔业生物技术与遗传育种重点实验室青岛市海水鱼类种子工程与生物技术重点实验室青岛266071 上海海洋大学水产与生命学院上海201306 青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室青岛266071 烟台开发区天源水产有限公司烟台264003

在鱼类适应环境盐度变化的过程中,鳃、肾、肠是主要的渗透调节器官,而水通道蛋白(Aquaporins, AQPs)、囊性纤维化跨膜传导调节因子(CFTR)、钠氢交换体(NHE)又是这些器官中重要的渗透调节基因。为研究AQP1、AQP3、CFTR、NHE1在大菱鲆(Sco... 详细信息

在鱼类适应环境盐度变化的过程中,鳃、肾、肠是主要的渗透调节器官,而水通道蛋白(Aquaporins, AQPs)、囊性纤维化跨膜传导调节因子(CFTR)、钠氢交换体(NHE)又是这些器官中重要的渗透调节基因。为研究AQP1、AQP3、CFTR、NHE1在大菱鲆(Scophthalmusmaximus)低盐胁迫过程中的渗透调节功能,本研究采用荧光定量PCR技术,对4种基因在盐度5和盐度10下大菱鲆鳃、肾、肠中表达量随时间的变化进行检测。结果显示,AQP1表达量在鳃中极少(P<0.05),在肾和肠中较高,低盐胁迫下,盐度5组和盐度10组在鳃中的表达量无显著变化,在肾和肠中均显著上升(P<0.05)。AQP3表达量在肾中极少(P<0.05),在鳃中较高,在肠中较少,低盐胁迫下,盐度5组和盐度10组在肾中的表达量无显著变化,在鳃和肠中均显著上升(P<0.05)。CFTR表达量在肾中极少,在鳃中较高,在肠中较少,低盐胁迫下,盐度5组和盐度10组在肾中的表达量无显著变化,在鳃和肠中均显著下降(P<0.05)。NHE1在鳃和肠中表达量较少,在肾中较高,低盐胁迫下,盐度5组和盐度10组在鳃中的表达量无显著变化,在肾和肠中均显著上升(P<0.05)。这些结果表明,4种基因表达水平因组织、盐度和时间的不同而不同,反映了这4种基因的功能特异性;在低盐胁迫下,4种基因积极响应,表达量均发生不同程度的变化,表明AQP1、AQP3、CFTR和NHE1在大菱鲆低盐环境适应中可能具有潜在的重要作用。另外,本研究结果可为大菱鲆半咸水养殖和淡化养殖提供理论依据,同时为培育适应低盐环境大菱鲆良种提供理论和技术支撑。

关键词：大菱鲆低盐胁迫水通道蛋白囊性纤维化跨膜传导调节因子钠氢交换体荧光定量PCR

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