Hypothalamic-Pituitary-Gonadal(HPG)Axis and Transcriptional Regulatory Elements Regulate piwil2 Gene Expression During Gametogenesis and Gonadal Development in Japanese Flounder(Paralichthys olivaceus)

作     者：NI Feifei YU Haiyang QU Jiangbo MENG Lihui LIU Xiumei YAN Weijie CHANG Jing ZHANG Quanqi WANG Xubo YU Haiyang NI Feifei;YU Haiyang;QU Jiangbo;MENG Lihui;LIU Xiumei;YAN Weijie;CHANG Jing;ZHANG Quanqi;WANG Xubo

作者机构：Key Laboratory of Marine Genetics and BreedingMinistry of EducationOcean University of ChinaQingdao 266003China College of Life SciencesYantai UniversityYantai 264005China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2020年第19卷第6期

页      面：1378-1388页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：This study was supported by the National Natural Science Foundation of China(No.31672646) the Natural Science Foundation of Shandong Province(No.ZR 2017MC072) 

主　　题：piwil2 gametogenesis and gonadal development HPG axis transcriptional regulatory elements Paralichthys olivaceus 

摘      要：The P-element induced wimpy testis(Piwi)proteins,which are associated with PIWI-interacting RNAs(piRNAs),play important roles in meiosis,germ cell division,and germline *** this study,we identified and characterized the Paralichthys olivaceus piwil2 gene,a constituent factor of the piRNA pathways involved in the biogenesis of reproductive *** biological analysis indicated that piwil2,which contains PAZ and PIWI domains,was highly conserved between teleosts and *** piwil2 distribution profile in different tissues confirmed a sexually dimorphic expression pattern,with a higher expression level in *** situ hybridization demonstrated that piwil2 was expressed in the oogonia and oocytes of the ovaries as well as in the Sertoli cells and spermatocytes of the *** piwil2 showed a maternally inherited expression pattern during embryonic development,and was highly expressed during the early embryonic *** luciferase reporters were constructed to determine the transcriptional regulatory mechanisms of *** piwil2 core promoter region was located at−360 bp to−60 ***,some representative sex hormones,including human chorionic gonadotropin,17α-methyltestosterone,and estradiol-17βhad distinct regulatory effects on *** a summery,these results indicate that piwil2,regulated by sex hormones and transcriptional elements,has vital functions in the reproductive cycle and gonadal development.