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Coronavirus transmissible gastroenteritis virus antagonizes the antiviral effect of the microRNA miR-27b via the IRE1 pathway

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Science China(Life Sciences) 2022年第7期65卷 1413-1429页

作者： Changlin Wang Mei Xue Peng Wu Honglei Wang Zhongqing liu Guangzheng Wu pinghuang liu Keliang Wang Wanhai Xu Li Feng Department of Urology the Fourth Affiliated Hospital of Harbin Medical UniversityHarbin 150001China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China NHC Key Laboratory of Molecular Probes and Targeted Diagnosis and Therapy Harbin Medical UniversityHarbin 150001China

Although the functional parameters of micro RNAs(mi RNAs)have been explored to some extent,the roles of these molecules in coronavirus infection and the regulatory mechanism of mi RNAs in virus infection are still *** gastroenteritis virus(TGEV)is an enteropathgenic coronavirus and causes high morbidity and mortality in suckling ***,we demonstrated that microRNA-27b-3p(miR-27b-3p)suppressed TGEV replication by directly targeting porcine suppressor of cytokine signaling 6(SOCS6),while TGEV infection downregulated miR-27b-3p expression in swine testicular(ST)cells and in ***,the decrease of miR-27b-3p expression during TGEV infection was mediated by the activated inositolrequiring enzyme 1(IRE1)pathway of the endoplasmic reticulum(ER)*** studies showed that when ER stress was induced by TGEV,IRE1 acted as an RNase activated by autophosphorylation and unconventionally spliced m RNA encoding a potent transcription factor,X-box-binding protein 1(Xbp1s).Xbp1s inhibited the transcription of miR-27 and ultimately reduced the production of ***,our findings indicate that TGEV inhibits the expression of an anti-coronavirus micro RNA through the IRE1 pathway and suggest a novel way in which coronavirus regulates the host cell response to infection.

关键词： coronavirus transmissible gastroenteritis coronavirus(TGEV) micro RNA inositol-requiring enzyme 1(IRE1) immune evasion

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Next-generation sequencing and single-cell RT-PCR reveal a distinct variable gene usage of porcine antibody repertoire following PEDV vaccination

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Science China(Life Sciences) 2020年第8期63卷 1240-1250页

作者： Ren Li Fang Fu Li Feng pinghuang liu State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001China

Porcine epidemic diarrhea virus(PEDV)is the most common diarrhea-causing pathogen in newborn *** clarifications of the overall antibody repertoire and antigen-specific antibody repertoire are essential to provide important insights into the B-cell response and reshape new ***,we applied next-generation sequencing(NGS)technology to investigate immunoglobulin(Ig)variable(V)gene segment usage of swine B-cells from peripheral blood lymphocytes(PBL)and mesenteric lymph node(MLN)cells following PEDV *** identified the transcripts of all functional Ig V-genes in antibody ***1 S2,IgKV1-11,and IgLV3-4 were the most prevalent gene segments for heavy,kappa,and lambda chains,respectively,in PBL and *** previous studies,IgKV1,instead of IgKV2,and IgLV3,instead of IgLV8,were the prevalent Ig V-gene families for kappa and lambda light chains,*** further examined the antibody repertoire of PEDV spike-specific B cells by single-cell *** contrast to the overall antibody repertoire,Ig V-gene segments of PEDV spike-specific B cells preferentially adopted IgHV1-4 and IgHV1-14 for heavy chain,IgKV1-11 for kappa chain,and IgLV3-3 for lambda *** results represent a comprehensive analysis to characterize the Ig V-gene segment usage in the overall and PEDV spike-specific antibody repertoire in PBL and MLN.

关键词： porcine epidemic diarrhea virus(PEDV) next-generation sequencing(NGS) single-cell RT-PCR antibody repertoire

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Isolate PEDV Specific Whole-porcine Neutralizing Antibody from Porcine Memory B cell through Single B cell PCR

Isolate PEDV Specific Whole-porcine Neutralizing Antibody fr...

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第十一届全国免疫学学术大会

作者： liu pinghuang Li lin Harbin Veterinary Research Institute

Introduction:single B-cell PCR antibody technology is currently the most advanced antibody *** technology has been successfully applied to isolate broadly neutralizing antibodies against several major human RNA viruses like HIV-1 and MERS,but it has not yet been applied in the veterinary *** epidemic diarrhea virus(PEDV)is economically important swine enteropathogenic coronaviruses,causing vomiting,watery diarrhea,

关键词： PEDV Neutralizing antibody Single B Cell PCR

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The dichotomous and incomplete adaptive immunity in COVID-19 patients with different disease severity

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Signal Transduction and Targeted Therapy 2021年第4期6卷 1292-1301页

作者： Leiqiong Gao Jing Zhou Sen Yang Lisha Wang Xiangyu Chen Yang Yang Ren Li Zhiwei Pan Jing Zhao Zhirong Li Qizhao Huang Jianfang Tang Li Hu pinghuang liu Guozhong Zhang Yaokai Chen Lilin Ye Institute of Immunology Third Military Medical UniversityChongqingChina Chongqing Public Health Medical Center ChongqingChina State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbinHeilongjiangChina College of Veterinary Medicine Northeast Agricultural UniversityHarbinHeilongjiangChina Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary MedicineChina Agricultural UniversityBeijingChina Cancer Center The General Hospital of Western Theater CommandChengduSichuanChina Comparative Immunology Research Center College of Veterinary MedicineChina Agricultural UniversityBeijingChina

The adaptive immunity that protects patients from coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is not well *** particular,the asymptomatic patients have been found to induce weak and transient SARS-CoV-2 antibody responses,but the underlying mechanisms remain unknown;meanwhile,the protective immunity that guide the recovery of these asymptomatic patients is ***,we characterized SARS-CoV-2-specific B-cell and T-cell responses in 10 asymptomatic patients and 64 patients with other disease severity(mild,n=10,moderate,n=32,severe,n=12)and found that asymptomatic or mild symptomatic patients failed to mount virus-specific germinal center(GC)B cell responses that result in robust and prolonged humoral immunity,assessed by GC response indicators including follicular helper T(TFH)cell and memory B cell responses as well as serum CXCL13 ***,these patients mounted potent virus-specific TH1 and CD8+T cell *** sharp contrast,patients of moderate or severe disease induced vigorous virus-specific GC B cell responses and associated TFH responses;however,the virus-specific TH1 and CD8+T cells were minimally induced in these *** results,therefore,uncovered the protective immunity in asymptomatic patients and also revealed the strikingly dichotomous and incomplete humoral and cellular immune responses in COVID-19 patients with different disease severity,providing important insights into rational design of effective COVID-19 vaccines.

关键词： immunity patients severity

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A novel linear and broadly neutralizing peptide in the SARS-CoV-2 S2 protein for universal vaccine development

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Cellular & Molecular Immunology 2021年第11期18卷 2563-2565页

作者： Tuofan Li Qiuqi Kan Jinying Ge Zhimin Wan Mengqi Yuan Yao Huang Quan Xie Yi Yang Hongxia Shao Xiangdong Li Lilin Ye Aijian Qin Zhigao Bu pinghuang liu Jianqiang Ye Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou225009JiangsuChina Key Laboratory of Jiangsu Preventive Veterinary Medicine Key Laboratory for Avian Preventive MedicineMinistry of EducationCollege of Veterinary MedicineYangzhou UniversityYangzhou225009JiangsuChina Joint International Research Laboratory of Agriculture and Agri-Product Safety the Ministry of Education of ChinaYangzhou UniversityYangzhou225009JiangsuChina Institutes of Agricultural Science and Technology Development Yangzhou UniversityYangzhou225009JiangsuChina State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural Sciences150069HarbinChina National High Containment Laboratory for Animal Diseases Control and Prevention 150069HarbinChina College of Veterinary Medicine China Agricultural University100193BeijingChina Center of Diseases Control and Prevention Yangzhou225009JiangsuChina Institute of Immunology PLAThird Military Medical University400038ChongqingChina

As humans continue to develop COVID-19 widely,numerous novel variants of SARS-CoV-2 have emerged[1,2].These variants,which may possess enhanced transmissibility and often result in breakthrough infections in the vaccinated population,pose great challenges to the current vaccine strategies targeting the immunodominance of the receptor-binding domain(RBD)of the spike(S)protein[2].

关键词： vaccine neutral breakthrough

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Disease severity dictates SARS-CoV-2-specific neutralizing antibody responses in COVID-19

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Signal Transduction and Targeted Therapy 2020年第1期5卷 921-926页

作者： Xiangyu Chen Zhiwei Pan Shuai Yue Fei Yu Junsong Zhang Yang Yang Ren Li Bingfeng liu Xiaofan Yang Leiqiong Gao Zhirong Li Yao Lin Qizhao Huang Lifan Xu Jianfang Tang Li Hu Jing Zhao pinghuang liu Guozhong Zhang Yaokai Chen Kai Deng Lilin Ye Institute of Immunology PLAThird Military Medical University400038 ChongqingChina Institute of Human Virology Key Laboratory of Tropical Disease Control of Ministry of EducationZhongshan School of MedicineSun Yat-sen University510080 GuangzhouChina Guangdong Provincial People’s Hospital Guangdong Academy of Medical Sciences510080 GuangzhouChina State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural Sciences150001 HarbinHeilongjiangChina College of Veterinary Medicine Northeast Agricultural University150030 HarbinHeilongjiangChina Cancer Center The General Hospital of Western Theater Command610083 ChengduSichuanChina Biomedical Analysis Center Third Military Medical University400038 ChongqingChina Comparative Immunology Research Center College of Veterinary MedicineChina Agricultural University100193 BeijingChina Key Laboratory of Animal Epidemiology of the Ministry of Agriculture College of Veterinary MedicineChina Agricultural University100193 BeijingChina Chongqing Public Health Medical Center 400038 ChongqingChina Guangzhou Eighth People’s Hospital Guangzhou Medical University510050 GuangzhouChina

COVID-19 patients exhibit differential disease severity after SARS-CoV-2 *** is currently unknown as to the correlation between the magnitude of neutralizing antibody(NAb)responses and the disease severity in COVID-19 *** a cohort of 59 recovered patients with disease severity including severe,moderate,mild,and asymptomatic,we observed the positive correlation between serum neutralizing capacity and disease severity,in particular,the highest NAb capacity in sera from the patients with severe disease,while a lack of ability of asymptomatic patients to mount competent ***,the compositions of NAb subtypes were also different between recovered patients with severe symptoms and with mild-tomoderate *** results reveal the tremendous heterogeneity of SARS-CoV-2-specific NAb responses and their correlations to disease severity,highlighting the needs of future vaccination in COVID-19 patients recovered from asymptomatic or mild illness.

关键词： severity neutral mount

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Isolate PEDV Specific Whole-porcine Neutralizing Antibody from Porcine Memory B cell through Single B cell PCR

Isolate PEDV Specific Whole-porcine Neutralizing Antibody fr...

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中国畜牧兽医学会生物技术学分会暨中国免疫学会兽医免疫分...

作者： Fang Fu Lin Li Li Feng pinghuang liu Harbin Veterinary Research Institute(HVRI) Chinese Academy of Agricultural Sciences(CAAS)

Single B-cell PCR antibody technology is currently the most advanced antibody *** technology has been successfully applied to isolate broadly neutralizing antibodies against several major human RNA viruses like HIV-1 and MERS,but it has not yet been applied in the veterinary *** epidemic diarrhea virus(PEDV)is economically im-

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IL-22 Suppresses the Infection of Porcine Enteric Coronaviruses by Activating STAT3 Signal Pathway

IL-22 Suppresses the Infection of Porcine Enteric Coronaviru...

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第十二届全国免疫学学术大会

作者： liu pinghuang Xue Mei Zhiao Jing Ma Yanlong Feng Li Harbin Veterinary Research Institute Qinghai University

Background:Interleukin-22(IL-22),a member of the IL-10 superfamily,is primarily secreted by T helper 17 cells,innate lymphoid cells(ILC),innate natural killer(INK)cells,and epithelial ***-22 primarily targets nonhematopoietic epithelial cells and fibroblasts and plays essential roles in fighting against mucosal microbial infection and

关键词： Interleukin 22 PEDV TGEV STAT3

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Frequency Regulation of VSC-MTDC System with Offshore Wind Farms

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Journal of Modern Power Systems and Clean Energy 2024年第1期12卷 275-286页

作者： Haoyu liu Chongru liu North China Electric Power University Beijing 102206China

Frequency regulation of voltage source converter-based multi-terminal high-voltage direct current(VSC-MTDC)system with offshore wind farms enhances the frequency stability by compensating the power for a disturbed AC ***,it is difficult to reasonably allocate frequency-regulation resources due to a lack of coordination mechanisms between wind farms and the MTDC ***,it is difficult for the frequency control of the wind farms to manage changes in wind speed;and the risk of wind-turbine stalls is ***,based on the kinetic energy of wind turbines and the power margin of the converters,the frequency-regulation capability of wind turbines is evaluated,and a dynamic frequency-support scheme considering the real-time frequency-support capability of the wind turbines and system frequency evolution is proposed to improve the frequency-support performance.A power adaptation technique at variable wind speeds is developed;the active power in the frequency-support stage and restoration stage is switched according to the wind speed.A hierarchical zoning frequency-regulation scheme is designed to use the frequency-regulation resources of different links in the MTDC system with wind *** simulation results show that the novel frequency-regulation strategy maintains frequency stability with wind-speed changes and avoids multiple frequency dips.

关键词： Wind generation voltage source converter-based multi-terminal high-voltage direct current(VSC-MTDC) frequency regulation cooperative control adaptive control variable wind speed

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A Novel Design of a Pre-separation Panel Structure to Protect Against the Impact of an Explosion in an Aircraft Plenum Chamber

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Acta Mechanica Solida Sinica 2024年第1期37卷 166-180页

作者： Zongxing liu Xiang Yang Jun liu School of Aeronautics Northwestern Polytechnical UniversityXi'an 710072China COMAC Shanghai Aircraft Design and Research Institute Shanghai 201210China

To reduce the damage of the pressurizing panel structure of a fuselage caused by an explosion at the“least risk bomb location”in an aircraft structure,a new pre-separation panel structure was designed to resist blast ***,the dynamic strain response and morphology of impact damage of the new pre-separation panel were measured in an impact damage ***,the commercial software LS-DYNA was used to calculate the propagation of the blast shock wave,and the results were compared with empirical equations to verify the rationality of the numerical calculation ***,the fluid–structure coupling method was used to calculate the damage process of the pre-separation panel structure under the impact of an explosion wave and an impact *** calculated results were in good agreement with the test results,which showed the rationality of the calculation method and the *** residual strength of the damaged pre-separation panel was significantly higher than that of the original damaged *** results show that the new pre-separation panel structure is reasonable and has certain significance for guiding the design of plenum chambers with strong resistance to implosion for aircraft fuselages.

关键词： Pre-separation panel structure Explosive response Residual strength Aircraft plenum chamber Least risk bomb location

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