Next-generation sequencing and single-cell RT-PCR reveal a distinct variable gene usage of porcine antibody repertoire following PEDV vaccination

作     者：Ren Li Fang Fu Li Feng PingHuang Liu Ren Li;Fang Fu;Li Feng;PingHuang Liu

作者机构：State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2020年第63卷第8期

页      面：1240-1250页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 090602[农学-预防兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Natural Science Foundation of China(31772718) the Open Research Fund of State Key Laboratory of Veterinary Biotechnology(SKLVBF2018XX) 

主　　题：porcine epidemic diarrhea virus(PEDV) next-generation sequencing(NGS) single-cell RT-PCR antibody repertoire 

摘      要：Porcine epidemic diarrhea virus(PEDV)is the most common diarrhea-causing pathogen in newborn *** clarifications of the overall antibody repertoire and antigen-specific antibody repertoire are essential to provide important insights into the B-cell response and reshape new ***,we applied next-generation sequencing(NGS)technology to investigate immunoglobulin(Ig)variable(V)gene segment usage of swine B-cells from peripheral blood lymphocytes(PBL)and mesenteric lymph node(MLN)cells following PEDV *** identified the transcripts of all functional Ig V-genes in antibody ***1 S2,IgKV1-11,and IgLV3-4 were the most prevalent gene segments for heavy,kappa,and lambda chains,respectively,in PBL and *** previous studies,IgKV1,instead of IgKV2,and IgLV3,instead of IgLV8,were the prevalent Ig V-gene families for kappa and lambda light chains,*** further examined the antibody repertoire of PEDV spike-specific B cells by single-cell *** contrast to the overall antibody repertoire,Ig V-gene segments of PEDV spike-specific B cells preferentially adopted IgHV1-4 and IgHV1-14 for heavy chain,IgKV1-11 for kappa chain,and IgLV3-3 for lambda *** results represent a comprehensive analysis to characterize the Ig V-gene segment usage in the overall and PEDV spike-specific antibody repertoire in PBL and MLN.