Coronavirus transmissible gastroenteritis virus antagonizes the antiviral effect of the microRNA miR-27b via the IRE1 pathway

作     者：Changlin Wang Mei Xue Peng Wu Honglei Wang Zhongqing Liu Guangzheng Wu Pinghuang Liu Keliang Wang Wanhai Xu Li Feng Changlin Wang;Mei Xue;Peng Wu;Honglei Wang;Zhongqing Liu;Guangzheng Wu;Pinghuang Liu;Keliang Wang;Wanhai Xu;Li Feng

作者机构：Department of Urologythe Fourth Affiliated Hospital of Harbin Medical UniversityHarbin 150001China State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China NHC Key Laboratory of Molecular Probes and Targeted Diagnosis and TherapyHarbin Medical UniversityHarbin 150001China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第7期

页      面：1413-1429页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the Heilongjiang Postdoctoral fund(LBH-Z18207) the National Natural Science Foundation of China(31802198) the Fundamental Research Funds for the Provincial Universities(2018-KYYWF-0553) the National Key Research and Development Program of China(2017YFC0908001) the Spark Research Fund from the Fourth Affiliated Hospital of Harbin Medical University(HYDSYXH201914) 

主　　题：coronavirus transmissible gastroenteritis coronavirus(TGEV) micro RNA inositol-requiring enzyme 1(IRE1) immune evasion 

摘      要：Although the functional parameters of micro RNAs(mi RNAs)have been explored to some extent,the roles of these molecules in coronavirus infection and the regulatory mechanism of mi RNAs in virus infection are still *** gastroenteritis virus(TGEV)is an enteropathgenic coronavirus and causes high morbidity and mortality in suckling ***,we demonstrated that microRNA-27b-3p(miR-27b-3p)suppressed TGEV replication by directly targeting porcine suppressor of cytokine signaling 6(SOCS6),while TGEV infection downregulated miR-27b-3p expression in swine testicular(ST)cells and in ***,the decrease of miR-27b-3p expression during TGEV infection was mediated by the activated inositolrequiring enzyme 1(IRE1)pathway of the endoplasmic reticulum(ER)*** studies showed that when ER stress was induced by TGEV,IRE1 acted as an RNase activated by autophosphorylation and unconventionally spliced m RNA encoding a potent transcription factor,X-box-binding protein 1(Xbp1s).Xbp1s inhibited the transcription of miR-27 and ultimately reduced the production of ***,our findings indicate that TGEV inhibits the expression of an anti-coronavirus micro RNA through the IRE1 pathway and suggest a novel way in which coronavirus regulates the host cell response to infection.