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Autologous Peripheral Blood Stem Cells and γ/δT Cells May Improve Immunity in Treating Secondary Bacteremic Infection in HIV Infected Patient

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Stem Cell discovery 2015年第4期5卷 48-61页

作者： Ciro Gargiulo Van Hung pham Nguyen Thuy Hai Kieu C. d. Nguyen Ngan duong Kim Thinh Nguyen Van An Luu Tuan Kenji Abe Veronica Flores Melvin Shiffman division of Internal Medicine The Human Medicine International Clinic Ho Chi Minh City Vietnam Molecular diagnostics department Nam Khoa-Biotek Laboratory Ho Chi Minh City Vietnam division of Pathology The Human Medicine International Clinic Ho Chi Minh City Vietnam department of Pathology National Institute of Infectious Diseases Tokyo Japa Sol Price School of Public Policy University of Southern California Los Angeles CA USA Section of Surgery Newport Specialty Hospital Tustin CA USA

Opportunistic bacteremia in adult HIV-infected patients is a normal co-infectious condition caused by Gram-negative bacilli. Respiratory infections, including cough, shortness of breath, and chest pain and skin infection with eruptions, pustules and itchiness, are common complaints in the setting of late HIV infection. The variety of infections ranges from mild, self-limited viral, bacteremia and fungal infections to severe, life-threatening demanding urgent care and hospitalization. Varicella pneumonia, for instance, is the most severe complication of chickenpox in HIV infected adults, potentially refractory, fulminant respiratory failure can ensue. Patients with impaired immune status and chronic lung disease are at an increased risk. In the United States as well as in Vietnam, bacterial/viral pneumonia and skin infection are the two most common HIV-associated conditions. While globally the incidence of opportunistic infection has decreased since the introduction of highly active antiretroviral therapy during the last 3 decades, HIV-associated diseases remain a significant source of mortality, thus any manifestation must be taken seriously. This study will present the most common HIV-related pulmonary and skin infections and provide an overview of the epidemiology, characteristic clinical and chest radiograph findings, diagnosis, treatment, and prevention globally as well in Vietnam. Though the extensive efforts of the Vietnamese Government during last decade contributed to a valuable decrease, yet epidemic in Vietnam still remains high, ranking Vietnam 5th in the South-East region. The second part of the study focuses on a unique and severe HIV case report of a 35-year-old man, with a rare form of pneumonia caused by Acitenobacter spp. concomitant with a prolonged and disseminating skin infection. The case has been treated with a combination of conventional anti-retroviral medication and autologous peripheral blood stem cells, the results showed that within 5 mon

关键词： Human Peripheral Blood Stem Cells γ/δ T Cells NK Cells Hematopoietic Stem Cells Cd4 Cd8 HIV

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母树径级与贮藏对胖大海种子发芽率的影响

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福建农林大学学报（自然科学版） 2017年第2期46卷 159-165页

作者：张巧巧 pham Van Huong 陈昌雄 LE Thi Hien 陈世品福建农林大学林学院福建福州350002 越南林业大学第二分校林学系越南同奈810000 西北农林科技大学林学院陕西杨凌712100

探讨母树径级、贮藏温度及贮藏时间对胖大海种子发芽率的影响.观察不同贮藏条件下种子的发芽情况,通过指数函数建立不同贮藏温度下胖大海种子发芽率与母树径级及贮藏时间的关系模型.结果表明:母树直径50~59 cm提供的种子发芽率、活力最... 详细信息

探讨母树径级、贮藏温度及贮藏时间对胖大海种子发芽率的影响.观察不同贮藏条件下种子的发芽情况,通过指数函数建立不同贮藏温度下胖大海种子发芽率与母树径级及贮藏时间的关系模型.结果表明:母树直径50~59 cm提供的种子发芽率、活力最大;母树直径d.综合分析表明,直径过大或过小的母树提供的种子发芽率均较低,从母树直径50~59 cm选种最佳,最好在0℃环境下贮藏种子,可以延长种子寿命至195 d,但胖大海种子不宜长久贮藏,优质的胖大海种子贮藏时间不能超过270 d.这为胖大海优种选择、贮藏及复壮生产提供了依据.

关键词：胖大海母树贮藏温度贮藏时间种子发芽率

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Mesenchymal stem cell-based therapy for ischemic stroke

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Chinese Neurosurgical Journal 2016年第4期2卷 233-238页

作者： Johnathon d.Anderson Missy T.pham Zelenia Contreras Madeline Hoon Kyle d.Fink Henrik J.Johansson Julien Rossignol department of Internal Medicine Stem Cell Program University of California Davis Davis USAInstitute for Regenerative Cures University of California Davis Medical Center 2921 Stockton Blvd Room 1300 Sacramento CA 95817 USA department of Internal Medicine Stem Cell Program University of California Davis Davis USA department of Oncology-Pathology Karolinska InstituteCancer Proteomics Davis USA College of Medicine Central Michigan University Mt PleasantUSA

Ischemic stroke represents a major,worldwide health burden with increasing *** affected by ischemic strokes currently have few clinically approved treatment options *** currently approved treatments for ischemic stroke have narrow therapeutic windows,severely limiting the number of patients able to be *** stem cells represent a promising novel treatment for ischemic *** studies have demonstrated that mesenchymal stem cells functionally improve outcomes in rodent models of ischemic *** studies have also shown that exosomes secreted by mesenchymal stem cells mediate much of this *** the present review,we summarize the current literature on the use of mesenchymal stem cells to treat ischemic *** studies investigating the mechanisms underlying mesenchymal stem cells tissue healing effects are warranted and would be of benefit to the field.

关键词： Mesenchymal stem cells Exosomes Ischemic stroke

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Ecoregional variations of aboveground biomass and stand structure in evergreen broadleaved forests

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Journal of Forestry Research 2020年第5期31卷 1713-1722页

作者： Tran Van do Mamoru Yamamoto Osamu Kozan Vo dai Hai Phung dinh Trung Nguyen Toan Thang Lai Thanh Hai Vu Thanh Nam Trieu Thai Hung Hoang Van Thang Tran duc Manh Cao Chi Khiem Vu Tien Lam Nguyen Quang Hung Tran Hoang Quy pham Quang Tuyen Trinh Ngoc Bon Nguyen Thi Thu Phuong Ninh Viet Khuong Nguyen Van Tuan dang Thi Hai Ha Tran Hai Long dang Van Thuyet dang Thinh Trieu Nguyen Van Thinh Tran Anh Hai duong Quang Trung Nguyen Van Bich dinh Hai dang pham Tien dung Nguyen Huy Hoang Le Thi Hanh Phan Minh Quang Nguyen Thi Thuy Huong Hoang Thanh Son Nguyen Thanh Son Nguyen Thi Van Anh Nguyen Thi Hoai Anh pham dinh Sam Hoang Thi Nhung Hoang Van Thanh Nguyen Huu Thinh Tran Hong Van Ho Trung Luong Bui Kieu Hung Research Institute for Sustainable Humanosphere Kyoto UniversityUjiKyotoJapan Silviculture Research Institute Vietnamese Academy of Forest SciencesNorth Tu LiemHanoiVietnam Center for Southeast Asian Studies Kyoto UniversityKyotoJapan Vietnamese Academy of Forest Sciences North Tu LiemHanoiVietnam Vietnam Administration of Forestry No.2 Ngoc HaBa DinhHanoiVietnam

Biotic and abiotic factors control aboveground biomass(AGB)and the structure of forest *** study analyses the variation of AGB and stand structure of evergreen broadleaved forests among six ecoregions of Vietnam.A data set of 1731-ha plots from 52 locations in undisturbed old-growth forests was *** results indicate that basal area and AGB are closely correlated with annual precipitation,but not with annual temperature,evaporation or hours of *** area and AGB are positively correlated with trees>30 cm *** areas surveyed(52.6%)in these old-growth forests had AGB of 100–200 Mg ha^-1;5.2%had AGB of 400–500 Mg ha^-1,and 0.6%had AGB of>800 Mg ha^-*** percent of the areas surveyed had stand densities of 300–600 ***^-1,and 64%had basal areas of 20–40 m^2 ha^-*** is an important factor influencing the AGB of old-growth,evergreen broadleaved forests in *** causing the loss of large-diameter trees(e.g.,>100 cm dBH)affects AGB but may not seriously affect stand density.

关键词： Aboveground biomass Carbon storage Climatic variables Ecoregion Edaphic variables Oldgrowth forest

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Type I and II interferons enhance dendritic cell maturation and migration capacity by regulating Cd38 and Cd74 that have synergistic effects with TLR agonists

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Cellular & Molecular Immunology 2011年第4期8卷 341-347页

作者： Thanh-Nhan Nguyen-pham Mi-Seon Lim Truc Anh Thi Nguyen Youn-Kyung Lee Chun-Ji Jin Hyun Ju Lee Cheol Yi Hong Jae-Sook Ahn deok-Hwan Yang Yeo-Kyeoung Kim Ik-Joo Chung Byoung Chul Park Hyeoung-Joon Kim Je-Jung Lee Research Center for Cancer Immunotherapy Chonnam National University Hwasun HospitalJeollanamdoKorea department of Hematology-Oncology Chonnam National UniversityGwangjuKorea The Brain Korea 21 Project Center for Biomedical Human Resources at Chonnam National UniversityGwangjuKorea Vaxcell-Bio Therapeutics JeollanamdoKorea Korea Research Institute of Bioscience and Biotechnology(KRIBB) DaejeonKorea

The major limitation for the maturation of dendritic cells(dCs)using Toll-like receptor(TLR)agonists is their decreased ability to migrate into lymph nodes compared with conventional ***38 can be used as a multifunctional marker to modulate migration,survival and Th1 responses of ***74 has been shown to negatively regulate dC *** goal of this study was to investigate the combinations of TLR agonists and interferons(IFNs)that most effectively regulate Cd38 and Cd74 expression on *** TLR agonist stimulation in combination with IFN-a and IFN-c was the best method for regulating Cd38 and Cd74 expression and inducing the highest secretion of *** in vitro migration assay showed that dCs treated with this combination had significantly enhanced migratory ability,similar to that observed in cells expressing Cd38,Cd74 and *** results of this study suggest that an alternative maturation protocol in which two TLR ligands are combined with type I and II IFNs generates potent dCs that have both a high migratory capacity and high IL-12p70 production.

关键词： Cd38 Cd74 dendritic cells interferon Toll-like receptor

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Controlling the Covalent and Noncovalent Adsorption of Proteins on Polymeric Surfaces in Auqeous Liquids

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光散射学报 2005年第3期17卷 234-236页

作者： E.P.Ivanova d.K.pham Y.V.Alekseeva G.M.demyashev d.V.Nicolau Industrial Research Institute Swinburne Swinburne University of TechnologyPO Box 218HawthornVic 3122Australia

The adsorption and covalent immobilization of human immunoglobulin(HIgG),lysozyme,α-chymotrypsin,and myoglobin have been compared using different experimental techniques:ellipsometry(ELM),X-ray photoelectron spectroscopy(XPS),optical fluorescence and atomic force microscopy(AFM).All measurements of the protein-surface interactions were performed with identically prepared Polysterine maleic acid(PSMA),Poly(methyl methacrylate)(PMMA),and Poly(tert-butyl methacrylate)P(tBuMA)polymeric *** via UV exposure PMMA and P(tBuMA)films were utilized to couple proteins via an N-hydroxysuccinimide(NHS)*** covalent binding was evaluated by XPS *** optical techniques showed a good correlation in terms of variable-dense protein layer formation(surface coverage range between 4-23 ng/mm2)of adsorbed and covalently bound *** covalent immobilization resulted in stable monolayer formation confirmed by ELM and AFM imaging,in contrast to rigorous interaction with surfaces of adsorbed proteins often resulted in multilayer *** results demonstrate that the covalent protein immobilization is an effective and robust way to achieve dense and stable protein monolayer formation.

关键词： P(tBuMA) All measurements PMMA

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水溶液中蛋白质在聚合物表面共价及非共价吸附的控制(英文)

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光散射学报 2005年第3期 46-48页

作者： E.P.Ivanova,d.K.pham,Y.V.Alekseeva,G.M.demyashev,d.V.Nicolau Industrial Research Institute Swinburne Swinburne University of Technology PO Box 218 Hawthorn Vic 3122 Australia Industrial Research Institute Swinburne Swinburne University of Technology PO Box 218 Hawthorn Vic 3122 Australia Industrial Research Institute Swinburne Swinburne University of Technology PO Box 218 Hawthorn Vic 3122 Australia Industrial Research Institute Swinburne Swinburne University of Technology PO Box 218 Hawthorn Vic 3122 Australia Industrial Research Institute Swinburne Swinburne University of Technology PO Box 218 Hawthorn Vic 3122 Australia

The adsorption and covalent immobilization of human immunoglobulin (HIgG), lysozyme, α-chymotrypsin, and myoglobin have been compared using different experimental techniques: ellipsometry (ELM), X-ray photoelectron spectroscopy (XPS), optical fluorescence and atomic force microscopy (AFM). All measurements of the protein-surface interactions were performed with identically prepared Polysterine maleic acid (PSMA), Poly(methyl methacrylate) (PMMA), and Poly(tert-butyl methacrylate) P(tBuMA) polymeric substrates. Functionalized via UV exposure PMMA and P(tBuMA) films were utilized to couple proteins via an N-hydroxysuccinimide (NHS) intermediate. The covalent binding was evaluated by XPS analysis. The optical techniques showed a good correlation in terms of variable-dense protein layer formation (surface coverage range between 4-23 ng/mm2) of adsorbed and covalently bound proteins. The covalent immobilization resulted in stable monolayer formation confirmed by ELM and AFM imaging, in contrast to rigorous interaction with surfaces of adsorbed proteins often resulted in multilayer formation. The results demonstrate that the covalent protein immobilization is an effective and robust way to achieve dense and stable protein monolayer formation.

关键词：共价 EdC XPS

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越南黎朝中兴时期升龙皇城的海阳瓷器

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故宫博物院院刊 2020年第9期 79-91,126页

作者： [越南]范氏莺张心仪(译) 越南社会科学院皇城研究所北京大学外国语学院

本文介绍了升龙皇城遗址(Hoàng thành Th?ng Long)出土的17-18世纪越南黎朝中兴时期海阳瓷器(甲、乙、丙、丁组),产地集中于平江制瓷中心的柿窑(C?y)、劳窑(Láo)和合礼窑(H?p L?)等。作者通过分析其特征、类型后,推断该时期升龙城本... 详细信息

本文介绍了升龙皇城遗址(Hoàng thành Th?ng Long)出土的17-18世纪越南黎朝中兴时期海阳瓷器(甲、乙、丙、丁组),产地集中于平江制瓷中心的柿窑(C?y)、劳窑(Láo)和合礼窑(H?p L?)等。作者通过分析其特征、类型后,推断该时期升龙城本地的窑口可能已经衰落,而民窑瓷器开始涌入宫廷,同时按照功能对这批瓷器进行类型归纳。通过对海阳瓷器的研究,可以看到当时升龙皇宫内贵族们嗜好饮酒、抽水烟、嚼槟榔等多样的生活面貌。

关键词：海阳瓷器黎中兴时期升龙皇城

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Phytochemical Constituents and determination of Resveratrol from the Roots of Arachis hypogeaL.

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American Journal of Plant Sciences 2013年第12期4卷 2351-2358页

作者： Tuyen pham Nguyen Kim Nga Vo Thi Phuong Tran Van Phuong Quach Ngo diem duong Ngo Thi Thuy Quang Ton That Phung Nguyen Kim Phi department of Chemical Technology Faculty of Chemical-Food Technology University of Technical Education Ho Chi Minh City Ho Chi Minh City Vietnam department of Organic Chemistry Faculty of Chemistry University of Science Vietnam National University-Ho Chi Minh City Ho Chi Minh City Vietnam department of Plant Biotech and Biotransformation Faculty of Biology University of Science Vietnam National University-Ho Chi Minh City Ho Chi Minh City Vietnam Faculty of Environmental Science Sai Gon University Ho Chi Minh City Vietnam

Two triterpenoid saponins, 6, 7, together with five known compounds were isolated from the roots of Arachis hypogea L. collected in Vietnam. Their structures were established by spectroscopic analysis (1d, 2d NMR and HRMS) as well as by comparison with those reported in the literature. Furthermore, the high content of resveratrol in the roots opens a new potential resource for pharmaceutical companies to prepare medicines from the wasted peanut roots.

关键词： Arachis hypogea L. Peanut Resveratrol Triterpenoid Glycoside

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Automated Segmentation and Connectivity Analysis for Normal Pressure Hydrocephalus

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Biomedical Engineering Frontiers 2022年第1期3卷 1-13页

作者： Angela Zhang Amil Khan Saisidharth Majeti Judy pham Christopher Nguyen Peter Tran Vikram Iyer Ashutosh Shelat Jefferson Chen B.S.Manjunath Vision Research Laboratory Electrical and Computer EngineeringUniversity of CaliforniaSanta BarbaraSanta BarbaraCAUSA Chen Lab Department of NeurosurgeryUniversity of CaliforniaIrvine Medical CenterOrangeCAUSA Santa Barbara Cottage Hospital Santa BarbaraCAUSA

Objective and Impact *** propose an automated method of predicting Normal Pressure Hydrocephalus(NPH)from CT scans.A deep convolutional network segments regions of interest from the *** regions are then combined with MRI information to predict *** our knowledge,this is the first method which automatically predicts NPH from CT scans and incorporates diffusion tractography information for *** to their low cost and high versatility,CT scans are often used in NPH *** well-defined and effective protocol currently exists for analysis of CT scans for ***’index,an approximation of the ventricle to brain volume using one 2d image slice,has been proposed but is not *** proposed approach is an effective way to quantify regions of interest and offers a computational method for predicting *** propose a novel method to predict NPH by combining regions of interest segmented from CT scans with connectome data to compute features which capture the impact of enlarged ventricles by excluding fiber tracts passing through these *** segmentation and network features are used to train a model for NPH *** method outperforms the current state-of-the-art by 9 precision points and 29 recall *** segmentation model outperforms the current state-of-the-art in segmenting the ventricle,gray-white matter,and subarachnoid space in CT *** experimental results demonstrate that fast and accurate volumetric segmentation of CT brain scans can help improve the NPH diagnosis process,and network properties can increase NPH prediction accuracy.

关键词： diagnosis network Connectivity

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