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High density genetic map and quantitative trait loci(QTLs)associated with petal number and flower diameter identified in tetraploid rose

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Journal of Integrative Agriculture 2021年第5期20卷 1287-1301页

作者： YU Chao WAN Hui-hua peter m.bourke CHENG Bi-xuan LUO Le PAN Hui-tang ZHANG Qi-xiang Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/Engineering Research Center of Landscape Environment of ministry of Education/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of ministry of Education School of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083P.R.China Plant Breeding Wageningen University&ResearchWageningen 6700 AJThe Netherlands Beijing Advanced Innovation Center for Tree Breeding by molecular Design Beijing Forestry UniversityBeijing 100083P.R.China

Rose is one of the most important ornamental and economic plants in the *** rose cultivars are primarily tetraploid,and during meiosis,they may exhibit double reduction or preferential chromosome ***,the construction of a high density genetic map of tetraploid rose is both challenging and *** this study,a tetraploid rose population was used to conduct a genetic analysis using genome sequencing.A total of 17382 single nucleotide polymorphism(SNP)markers were selected from 2308042 detected *** with 440 previously developed simple sequence repeats(SSR)and amplified fragment length polymorphism(AFLP)markers,a marker dosage of 6885 high quality markers was successfully assigned by GATK software in the tetraploid *** markers were used in the construction of a high density genetic map,containing the expected seven linkage groups with 6842 markers,a total map length of 1158.9 c m,and an average inter-marker distance of 0.18 c *** trait locus(QTL)analysis was subsequently performed to characterize the genetic architecture of petal number and flower *** major QTL(qpnum-3-1)was detected for petal number in three consecutive years,which explained 20.18–22.11%of the variation in petal *** QTLs were detected for flower diameter;the main locus,qfdia-2-2,was identified in two consecutive *** results will benefit the molecular marker-assisted breeding of modern rose *** addition,this study provides a guide for the genetic and QTL analysis of autotetraploid plants using sequencing-based genotyping methods.

关键词： rose tetraploid genetic linkage map QTLs

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In the name of the rose:a roadmap for rose research in the genome era

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Horticulture Research 2019年第1期6卷 948-964页

作者： marinus J.m.Smulders Paul Arens peter m.bourke Thomas Debener marcus Linde Jan De Riek Leen Leus Tom Ruttink Sylvie Baudino Laurence Hibrant Saint-Oyant Jeremy Clotault Fabrice Foucher Plant Breeding Wageningen University and ResearchP.O.Box 3866700 AJ WageningenThe Netherlands Faculty of Natural Sciences Institute for Plant GeneticsMolecular Plant BreedingLeibniz University of HannoverHerrenhäuser Strasse 230419 HannoverGermany ILVO Plant Sciences UnitFlanders Research Institute for AgricultureFisheries and FoodCaritasstraat 399090 MelleBelgium BVpam CNRS FRE 3727UJM-Saint-ÉtienneUniv.LyonSaint-EtienneFrance IRHS Agrocampus-OuestINRAUniversitéd’AngersSFR 4207 QuaSaV42 rue Georges Morel BP 6005749071 BeaucouzéFrance

The recent completion of the rose genome sequence is not the end of a process,but rather a starting point that opens up a whole set of new and exciting *** to a high-quality genome sequence other genomic tools have also become available for rose,including transcriptomics data,a high-density single-nucleotide polymorphism array and software to perform linkage and quantitative trait locus mapping in *** cultivars are highly heterogeneous and *** vast diversity in cultivated roses can be explained through the genetic potential of the genus,introgressions from wild species into commercial tetraploid germplasm and the inimitable efforts of historical *** can now investigate how this diversity can best be exploited and refined in future breeding work,given the rich molecular toolbox now available to the rose breeding *** paper presents possible lines of research now that rose has entered the genomics era,and attempts to partially answer the question that arises after the completion of any draft genome sequence:‘Now that we have“the”genome,what’s next?’.Having access to a genome sequence will allow both(fundamental)scientific and(applied)breeding-orientated questions to be *** outline possible approaches for a number of these questions.

关键词： breeding cultivar linkage

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从睡眠障碍中苏醒

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科学新闻 2023年第4期 59-60页

作者： Ashley m.bourke 倪伟波马克斯·普朗克脑研究所

作为博士后，在第一次研讨会上向实验室小组作报告之前，我的眼皮变得沉重，警觉性消失在一种梦幻般的状态中。这是一种睡眠发作——由嗜睡症引起，两年前我被诊断出患有这种病。我为自己不停地打哈欠道歉，尽管非常想睡觉，但我还得继...

作为博士后，在第一次研讨会上向实验室小组作报告之前，我的眼皮变得沉重，警觉性消失在一种梦幻般的状态中。这是一种睡眠发作——由嗜睡症引起，两年前我被诊断出患有这种病。我为自己不停地打哈欠道歉，尽管非常想睡觉，但我还得继续演讲，这是嗜睡症让我的事业举步维艰的众多时刻之一。但自从听说一位著名科学家也患有嗜睡症后，我知道自己也能在科学上取得成功。我一生中大部分时间都在与想睡觉的冲动作斗争。

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High-density SNP-based genetic maps for the parents of an outcrossed and a selfed tetraploid garden rose cross, inferred from admixed progeny using the 68k rose SNP array

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Horticulture Research 2016年第1期3卷 68-75页

作者： mirjana Vukosavljev Paul Arens Roeland E Voorrips Wendy P C van't Westende G D Esselink peter m.bourke peter Cox W Eric van de Weg Richard G F Visser Chris maliepaard marinus J m Smulders Wageningen UR Plant Breeding Wageningen University&ResearchWageningenNL-6700 AJThe Netherlands Roath BV EindhovenThe Netherlands Pheno Geno Roses D.O.O.Bulevar oslobođenja 65a 21000 Novi SadSerbia

Dense genetic maps create a base for QTL analysis of important traits and future implementation of marker-assisted *** tetraploid rose,the existing linkage maps include<300 markers to cover 28 linkage groups(4 homolog... 详细信息

Dense genetic maps create a base for QTL analysis of important traits and future implementation of marker-assisted *** tetraploid rose,the existing linkage maps includemarkers to cover 28 linkage groups(4 homologous sets of 7 chromosomes).Here we used the 68k WagRhSNP Axiom single-nucleotide polymorphism(SNP)array for rose,in combination with SNP dosage calling at the tetraploid level,to genotype offspring from the garden rose cultivar‘Red New Dawn’.The offspring proved to be not from a single bi-parental *** rose breeding,crosses with unintended parents occur *** developed a strategy to separate progeny into putative populations,even while one of the parents was unknown,using principle component analysis on pairwise genetic distances based on sets of selected SNP markers that were homozygous,and therefore uninformative for one *** of the inferred populations was consistent with self-fertilization of‘Red New Dawn’.Subsequently,linkage maps were generated for a bi-parental and a self-pollinated population with‘Red New Dawn’as the common maternal *** densest map,for the selfed parent,had 1929 SNP markers on 25 linkage groups,covering 1765.5 cm at an average marker distance of 0.9 *** with the strawberry(Fragaria vesca)genome was *** ICm1 corresponded to *** pseudochromosome 7(Fv7),ICm4 to Fv4,ICm5 to Fv3,ICm6 to Fv2 and ICm7 to *** ICm2 corresponded to parts of *** pseudochromosomes 1 and 6,whereas ICm3 is syntenic to the remainder of Fv6.

关键词： breeding linkage dosage

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Target blood pressure in diabetes patients with hypertension——What is the accumulated evidence in 2011?

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第8期12卷 611-623页

作者： peter m.NILSSON Department of Clinical Sciences Lund UniversityUniversity Hospital

There is overwhelming evidence that hypertension is an important risk factor for both macrovascular and microvascular complications in patients with diabetes,but the problem remains to identify appropriate goals for preventive therapies.A number of guidelines (the European Society of Cardiology (ESC)/European Association for the Study of Diabetes (EASD) 2007,the Joint National Committee (JNC)-Ⅶ 2003,the American Diabetes Association (ADA) 2011) have for example advocated a blood pressure goal of less than 130/80 mmHg,but this suggestion has been challenged by findings in recent trials and meta-analyses (2011).The European Society of Hypertension (ESH) therefore recommends a systolic blood pressure goal of "well below" 140 *** on evidence from both randomized controlled trials (hypertension optimal treatment (HOT),action in diabetes and vascular disease:preterax and diamicron mR controlled evaluation (ADVANCE),action to control cardiovascular risk in diabetes (ACCORD)) and observational studies (ongoing telmisartan alone and in combination with ramipril global endpoint trial (ONTARGET),international verapamil-trandolapril study (INVEST),treat to new targets (TNT),and the National Diabetes Register (NDR)),it has been shown that the benefit for stroke reduction remains even at lower achieved blood pressure levels,but the risk of coronary events may be uninfluenced or even increased at lower systolic blood pressure *** a recent meta-analysis,it was therefore concluded that the new recommended goal should be 130-135 mmHg systolic blood pressure for most patients with type 2 *** risk factors should also be controlled with a more ambitious strategy applied in the younger patients with shorter diabetes duration,but a more cautious approach in the elderly and frail patients with a number of vascular or non-vascular *** patients from East Asia,such as China,the stroke risk is relatively higher than the risk of coronary *** must al

关键词： Blood pressure Cardiovascular Diabetes Goal Hypertension Treatment

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Quantum corrections for Boltzmann equation

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Science China(Physics,mechanics & Astronomy) 2008年第9期51卷 1229-1239页

作者： m. Levy peter Department of Physics New York University

We present the lowest order quantum correction to the semiclassical Boltzmann distribution function,and the equation satisfied by this correction is given. Our equation for the quantum correction is obtained from the conventional quantum Boltzmann equation by explicitly expressing the Planck constant in the gradient approximation,and the quantum Wigner distribution function is expanded in pow-ers of Planck constant,too. The negative quantum correlation in the Wigner dis-tribution function which is just the quantum correction terms is naturally singled out,thus obviating the need for the Husimi’s coarse grain averaging that is usually done to remove the negative quantum part of the Wigner distribution function. We also discuss the classical limit of quantum thermodynamic entropy in the above framework.

关键词： quantum Boltzmann equation Wigner distribution function quantum correction

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Testing flexible polymer solar cells in near-space

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National Science Review 2023年第5期10卷 105-106页

作者： peter müller-Buschbaum Department of Physics TUM School of Natural Sciencesand Heinz Maier-Leibnitz-Zentrum(MLZ)Technical University of Munich

Since the big success of the Vanguard1 mission,being the first solar-powered satellite,the use of solar cells in space developed very ***,conventional solar cell technologies for space are based on silicon and GaAs **...

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Fluid and Osmotic Pressure Balance and Volume Stabilization in Cells Dedicated to Professor Karl Stark Pister for his 95th birthday

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Computer modeling in Engineering & Sciences 2021年第12期129卷 1329-1350页

作者： peter m.Pinsky Department of mechanical Engineering Stanford UniversityStanford94305CAUSA

A fundamental problem for cells with their fragile membranes is the control of their *** primordial solution to this problem is the active transport of ions across the cell membrane to modulate the intracellular osmotic *** this work,a theoretical model of the cellular pump-leak mechanism is proposed within the general framework of linear nonequilibrium *** model is expressed with phenomenological equations that describe passive and active ionic transport across cell membranes,supplemented by an equation for the membrane potential that accounts for the electrogenicity of the ionic *** active ionic transport,the model predicts that the intracellular fluid pressure will be balanced by the osmotic pressure and a new pressure component that arises from the active ionic fluxes.A model for the pump-leak mechanism in an idealized human cell is introduced to demonstrate the applicability of the proposed theory.

关键词： Pump-leak mechanism cell volume regulation active ion transport ion pump membrane transport cell mechanics modified Kedem-Katchalsky equations nonequilibrium thermodynamics phenomenological equations

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Terrestrial denitrification:challenges and opportunities

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Ecological Processes 2012年第1期1卷 1-11页

作者： peter m Groffman Cary Institute of Ecosystem Studies 2801 Sharon TurnpikeMillbrookNY 12545USA

Denitrification is a process of great environmental importance but is difficult to study in terrestrial *** for quantifying the process are problematic,variability in activity is high,and temporal and spatial scaling challenges are *** methods are problematic for a variety of reasons;they change substrate concentrations,disturb the physical setting of the process,lack sensitivity or are prohibitively costly in time and *** fundamentally,it is very difficult to quantify the dominant end-product(N_(2))of denitrification given its high background concentration in the *** and temporal variation in denitrification is high due to control of the process by multiple factors(oxygen,nitrate,carbon,pH,salinity,temperature etc.)that each vary in time and space.A particular challenge is that small areas(hotspots)and brief periods(hot moments)frequently account for a high percentage of N gas flux *** phenomena are challenging to account for in measurement,modeling and scaling *** need for scaling is driven by the fact that there is a need for information on this microscale process at the ecosystem,landscape and regional scales where there are concerns about nitrogen effects on soil fertility,water quality and air *** this review,I outline the key challenges involved with denitrification and then describe specific opportunities for making progress on these challenges including advances in measurement methods,new conceptual approaches for addressing hotspot and hot moment dynamics,and new remote sensing and geographic information system–based scaling *** of these opportunities suggests that we are poised to make great improvements in our understanding of terrestrial *** improvements will increase our basic science understanding of a complex biogeochemical process and our ability to manage widespread nitrogen pollution problems.

关键词： scaling process landscape

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Simulation and calibration of granules using the discrete element method

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Particuology 2014年第1期12卷 40-43页

作者： peter müller Jürgen Tomas Institute of Process Engineering Mechanical Process EngineeringOtto-von-Guericke University MagdeburgUniversitätsplatz 239106 MagdeburgGermany

The discrete element method （DEm）, developed by Cundall and 5track to solve geomecnamcai problems, is used to simulate the mechanical behavior of granules. According to the DEm, an individ ual granule can be modeled as a realistic mechanical system consisting of primary particles bonded by interaction forces. Cranulometric properties of the model material, zeolite 4A, have been measured to determine their macro properties. To investigate the compression behavior, a compression test was performed using a strength tester on single granules between two pistons. A modeled granule consisting of more than 22,000 primary particles was generated. The micro properties of the modeled granule have been precisely set to allow its macro properties to be equivalent to the macro properties of zeolite 4A granules. To calibrate the mechanical properties, diametrical compression was simulated using two rigid walls stressed at a constant stressing velocity, The force-displacement curve of the modeled granule at compression has been calibrated by the experimental curve of zeolite 4A.

关键词： Discrete element method material behavior Granulation

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