检索结果-南通市图书馆

The miRNA let-7al inhibits the expression of insulin-like growth factor 1 receptor （IGFIR） in prostate cancer PC-3 cells

引用

Asian Journal of Andrology 2013年第6期15卷 753-758,I0006页

作者： Li-Na Wang Wei-Wen Chen ju zhang Chao-Yang Li Chun-Yan Liu Iing Xue peng-ju zhang An-Li Jiang Institution of Biochemistry and Molecular Biology Medical School of Shandong University Jinan 250012 China

Reduced microRNA （miRNA） let-7a expression and the activation of insulin-like growth factor-1 receptor （IGFIR） signalling are both involved in prostate cancer and progression. In the present study, we demonstrated that the growth inhibitory effect of let-7al is directly related to targeting IGFIRgene expression in PC-3 cells. TargetScan predicted three potential target sites （T1, T2 and T3） of let-7a in the 3＇ untranslational region （3＇ UTR） of IGFIR mRNA. Real-time PCR, Western blot and luciferase reporter assays were used to detect the effects of let-7al overexpression or let-7al inhibitor on the IGFIRgene expression in PC-3 cells. The results indicated that let-7al could inhibit IGFIR expression by directly targeting the T1 and T2 sites in the 3＇ UTR of the IGFIR mRNA. We then used RT-PCR, luciferase reporter assays, 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-2H-tetrazolium bromide （MTT） assay, flow cytometry and Hoechst 33342 staining to examine whether let-7al-mediated inhibition of IGFIR expression also affects the IGFIR-mediated signalling events, including Elk1 activity and c-fos gene expression, proliferation, apoptosis and cell cycle. We demonstrated that let-7al-mediated IGFIR downregulation was accompanied by attenuation of Elk1 activity and c-fos expression, inhibition of cell proliferation, enhanced apoptosis and cell cycle arrest, and that loss function of let-7al via inhibition can upregulate IGF1R accompanied by an increase of Elk1 activity and c-fos expression, thereby enhancing cell proliferation. Altogether, these findings sueeest that let-7a mav be novel therapeutic candidate for Drostate cancer.

关键词： IGFIR microRNA (miRNA) prostate cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluation of latest TMPA and CMORPH satellite precipitation products over Yellow River Basin

引用

Water Science and Engineering 2016年第2期9卷 87-96页

作者： Shan-hu Jiang Meng Zhou Li-liang Ren Xue-rong Cheng peng-ju zhang State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University College of Hydrology and Water Resources Hohai University

The main objective of this study was to evaluate four latest global high-resolution satellite precipitation products(TMPA 3B42 RT, CMORPH,TMPA 3B42V7, and CMORPH_adj) against gauge observations of the Yellow River Basin from March 2000 to December 2012. The assessment was conducted with several commonly used statistical indices at daily and monthly scales. Results indicate that 3B42V7 and CMORPH_adj perform better than the near real-time products(3B42RT and CMORPH), particularly the 3B42V7 product. The adjustment by gauge data significantly reduces the systematic biases in the research products. Regarding the near real-time datasets, 3B42 RT overestimates rainfall over the whole basin, while CMORPH presents a mixed pattern with negative and positive values of relative bias in low- and high-latitude regions,respectively, and CMORPH performs better than 3B42 RT on the whole. According to the spatial distribution of statistical indices, these values are optimized in the southeast and decrease toward the northwest, and the trend is similar for the spatial distribution of the mean annual precipitation during the period from 2000 to 2012. This study also reveals that all the four products can effectively detect rainfall events. This study provides useful information about four mainstream satellite products in the Yellow River Basin, and the findings can facilitate the use of global precipitation measurement(GPM) data in the future.

关键词： Satellite precipitation TMPA CMORPH Yellow River Basin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Emodin induces apoptosis in human prostate cancer cell LNCaP

引用

Asian Journal of Andrology 2008年第4期10卷 625-634页

作者： Chun-Xiao Yu Xiao-Qian zhang Lu-Dong Kang peng-ju zhang Wei-Wen Chen Wen-Wen Liu Qing-Wei Liu Jian-Ye zhang Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan 250012 China Merck Sharp ＆ Dohme Beijing 100738 China Positron Emission Tomography and Computer Tomography （PET-CT） Center Shandong Provincial Hospital Jinan 250021 China

Aim： To elucidate effects and mechanisms of emodin in prostate cancer cells. Methods： Viability of emodin-treated LNCaP cells and PC-3 cells was measured by MTT assay. Following emodin treatments, DNA fragmentation was assayed by agarose gel electrophoresis. Apoptosis rate and the expression of Fas and FasL were assayed by flow cytometric analysis. The mRNA expression levels of androgen receptor （AR）, prostate-specific antigen （PSA）, p53, p21, Bcl-2, Bax, caspase-3, -8, -9 and Fas were detected by RT-PCR, and the protein expression levels of AR, p53 and p21 were detected by Western blot analysis. Results： In contrast to PC-3, emodin caused a marked increase in apoptosis and a decrease in cell proliferation in LNCaP cells. The expression of AR and PSA was decreased and the expression of p53 and p21 was increased as the emodin concentrations were increased. In the same time, emodin induced apoptosis of LNCaP cells through the upregulation of caspase-3 and -9, as well as the increase of Bax/Bcl-2 ratio. However, it did not involve modulation of Fas or caspase-8 protein expression. Conclusion： In prostate cancer cell line, LNCaP, emodin inhibites the proliferation by AR and p53-p21 pathways, and induces apoptosis via the mitochondrial pathway.

关键词： emodin prostate cancer LNCaP PC-3 proliferation androgen receptor p53 apoptosis mitochondrial pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Double enzyme mimetic activities of multifunctional Ag nanoparticle-decorated Co_(3)V_(2)O_(8)hollow hexagonal prismatic pencils for application in colorimetric sensors and disinfection

引用

Nano Materials Science 2024年第2期6卷 244-255页

作者： Ying Gao peng ju Yu zhang Yuxin zhang Xiaofan Zhai Jizhou Duan Baorong Hou Key Laboratory of Marine Environmental Corrosion and Bio-fouling Institute of OceanologyChinese Academy of SciencesQingdao266071PR China Key Laboratory of Marine Eco-Environmental Science and Technology Marine Bioresource and Environment Research CenterFirst Institute of OceanographyMinistry of Natural ResourcesQingdao266061PR China University of Chinese Academy of Sciences Beijing100049PR China Open Studio for Marine Corrosion and Protection Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266071PR China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao266071PR China College of Materials Science and Engineering Chongqing UniversityChongqing400044China

Since the catalytic activity of most nanozymes is still far lower than the corresponding natural enzymes,there is urgent need to discover novel highly efficient enzyme-like *** this work,Co_(3)V_(2)O_(8)with hollow hexagonal prismatic pencil structures were prepared as novel artificial enzyme *** were then decorated by photo-depositing Ag nanoparticles(Ag NPs)on the surface to further improve its catalytic *** Ag NPs decorated Co_(3)V_(2)O_(8)(ACVPs)showed both excellent oxidase-and peroxidase-like catalytic *** can oxidize the colorless 3,3’,5,5’-tetramethylbenzidine rapidly to induce a blue *** enhanced enzyme mimetic activities can be attributed to the surface plasma resonance(SPR)effect of Ag NPs as well as the synergistic catalytic effect between Ag NPs and Co_(3)V_(2)O_(8),accelerating electron transfer and promoting the catalytic *** were applied in constructing a colorimetric sensor,validating the occurrence of the Fenton reaction,and disinfection,presenting favorable catalytic *** enzyme-like catalytic mechanism was studied,indicating the chief role of⋅O_(2)-radicals in the catalytic *** work not only discovers a novel functional material with double enzyme mimetic activity but also provides a new insight into exploiting artificial enzyme mimics with highly efficient catalytic ability.

关键词： Co_(3)V_(2)O_(8) Ag NPs Enzyme mimetic Colorimetric sensor Disinfection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of a Positive Cis-Element Upstream of Human NKX3.1 Gene

引用

Acta Biochimica et Biophysica Sinica 2005年第11期37卷 773-778页

作者： An-Li JIANG peng-ju zhang Xiao-Yan HU Wei-Wen CHEN Feng KONG Zhi-Fang LIU Hui-Qing YUAN Jian-Ye zhang Department of Biochemistry,Medical School of Shandong University,Jinan 250012,China Department of Biochemistry Medical School of Shandong UniversityJinan 250012 China

NKX3.1 is a prostate-specific homeobox gene related to prostate development and *** this work,we aimed to identify precisely the functional cis-element in the 197 bp region(from-1032 to -836 bp)of the NKX3.1 promoter(from -1032 to +8bp),which was previously identified topresent positive regulatory activity on NKX3.1 expression,by deletion mutagenesis analysis and electro-phoretic mobility shift assay(EMSA).A 16 bp positive cis-element located between -920 and -905 bpupstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functionalpositive cis-element by *** will be important to further study the functions and regulatory mechanismsof this positive cis-element in NKX3.1 gene expression.

关键词： human NKX3.1 cis-acting element positive regulation mutagenesis

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Up-regulation of NKX3.1 Expression and Inhibition of LNCaP Cell Proliferation Induced by an Inhibitory Element Decoy

引用

Acta Biochimica et Biophysica Sinica 2005年第5期37卷 335-340页

作者： An-Li JIANG Xiao-Yan HU peng-ju zhang Mei-Lan HE Feng KONG Zhi-Fang LIU Hui-Qing YUAN Jian-Ye zhang Department of Biochemistry, Medical School of Shandong University,Jinan 250012, China Department of Biochemistry Medical School of Shandong UniversityJinan 250012 China

NKX3.1 is an androgen-regulated prostate-specific homeobox gene that is thought to play animportant role in prostate development and ***3.1 acts as a tumor suppressor gene specificallyin the ***-regulation of NKX3.1 gene offers a promising gene therapy for prostate *** strategy has been developed and is considered a useful tool for regulating gene expression and *** our previous studies,we identified a 20 bp inhibitory element upstream of the NKX3.1 *** this study,we focused on using the 20 bp inhibitory element decoy to block negative regulation of theNKX3.1 gene and to up-regulate NKX3.1 expression using synthetic double-stranded oligodeoxynucleotidesof the 20 bp inhibitory *** found in an electrophoretic mobility shift assay experiment that the 20 bpinhibitory decoy presented competitive binding to a specific binding protein of the 20 bp inhibitory element inprostate cancer cell line *** luciferase reporter gene assays,we found that the 20 bp inhibitory decoycould enhance NKX3.1 promoter activity,and RT-PCR and Western blot analysis revealed that NKX3.1expression was up-regulated effectively by the transfection with the 20 bp inhibitory ***,cell proliferation was inhibited by up-regulated NKX3.1 expression induced by the 20 bp inhibitory decoy.

关键词： human NKX3.1 gene cis-acting element negative regulation decoy oligodeoxynucleotide cell proliferation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Distributed dynamic task allocation for unmanned aerial vehicle swarm systems:A networked evolutionary game-theoretic approach

引用

Chinese Journal of Aeronautics 2024年第6期37卷 182-204页

作者： Zhe zhang ju JIANG Haiyan XU Wen-An zhang College of Automation Engineering Nanjing University of Aeronautics and AstronauticsNanjing 211106China College of Economics and Management Nanjing University of Aeronautics and AstronauticsNanjing 211106China College of Information Engineering Zhejiang University of TechnologyHangzhou 310023China

Task allocation is a key aspect of Unmanned Aerial Vehicle(UAV)swarm collaborative *** an continuous increase of UAVs’scale and the complexity and uncertainty of tasks,existing methods have poor performance in computing efficiency,robustness,and realtime allocation,and there is a lack of theoretical analysis on the convergence and optimality of the *** paper presents a novel intelligent framework for distributed decision-making based on the evolutionary game theory to address task allocation for a UAV swarm system in uncertain scenarios.A task allocation model is designed with the local utility of an individual and the global utility of the ***,the paper analytically derives a potential function in the networked evolutionary potential game and proves that the optimal solution of the task allocation problem is a pure strategy Nash equilibrium of a finite strategy ***,a PayOff-based Time-Variant Log-linear Learning Algorithm(POTVLLA)is proposed,which includes a novel learning strategy based on payoffs for an individual and a time-dependent Boltzmann *** former aims to reduce the system’s computational burden and enhance the individual’s effectiveness,while the latter can ensure that the POTVLLA converges to the optimal Nash equilibrium with a probability of *** simulation results show that the approach is optimal,robust,scalable,and fast adaptable to environmental changes,even in some realistic situations where some UAVs or tasks are likely to be lost and increased,further validating the effectiveness and superiority of the proposed framework and algorithm.

关键词： Task allocation Unmanned Aerial Vehicles(UAV) Game theory Log-linear learning Distributed optimization algorithm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Electronic origin of structural degradation in Li-rich transition metal oxides:The case of Li_(2)MnO_(3)and Li_(2)RuO_(3)

引用

Journal of Semiconductors 2024年第4期45卷 82-86页

作者： peng zhang Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics andOp toelec-tronic EngineeringShenzhen UniversityShenzhen 518060China

Li_(2)MnO_(3)and Li_(2)RuO_(3)represent two prototype Li-rich transition metal(TM)oxides as high-capacity cathodes for Li-ion batteries,which have similar crystal structures but show quite different cycling ***,based on the first-princi-ples calculations,we systematically studied the electronic structures and defect properties of these two Li-rich cathodes,in order to get more understanding on the structural degradation mechanism in Li-rich TM *** calculations indicated that the structural and cycling stability of Li_(2)MnO_(3)and Li_(2)RuO_(3)depend closely on their electronic structures,especially the energy of their highest occupied electronic states(HOS),as it largely determines the defect properties of these *** Li_(2)MnO_(3)with low-energy HOS,we found that,due to the defect charge transfer mechanism,various defects can form spontaneously in its host structure as Li ions are extracted upon delithiation,which seriously deteriorates its structural and cycling *** for Li_(2)RuO_(3),on the other hand,we identified that the high-energy HOS prevents it from the defect formation upon delithi-ation and thus preserve its cycling *** studies thus illustrated an electronic origin of the structural degradation in Li-rich TM oxides and implied that it is possible to improve their cycling performances by carefully adjusting their TM compo-nents.

关键词： defects Li-ion batteries first-principles calculations

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity

引用

Journal of Integrative Agriculture 2024年第6期23卷 1967-1978页

作者： Lijie Chen Qianze peng Xiaohua Du Weixing zhang ju’e Cheng Shu’e Sun Deyong zhang Pin Su Yong Liu Longping Branch College of BiologyHunan UniversityChangsha 410082China State Key Laboratory of Hybrid Rice/Institute of Plant Protection Hunan Academy of Agricultural SciencesChangsha 410125China

Plant virus causes massive crop losses ***,there is currently no effective measure to control plant viral ***,we identify an antiviral protein Rhp-PSP,produced by the bacterial Rhodopseudomonas palustris strain *** this study,we discover that the antiviral activity of Rhp-PSP relies on its endoribonuclease *** the arginine(R)residue at position 129 onto alanine(A)abolishs its endoribonuclease activity on coat protein(CP)RNA of tobacco mosaic virus(TMV),consequentially,compromises the antiviral activity of *** investigation demonstrates that,the mutant Rhp-PSP^(R129A)is unable to form the homotrimer as the wild type,indicating the importance of quaternary junction for the endoribonuclease *** of Rhp-PSP in Nicotiana benthamiana significantly enhances the resistance against TMV of seedlings,while expression of Rhp-PSP^(R129A)did not,confirming that endoribonuclease activity is responsible for the antiviral activity of *** addition,foliar spray of Rhp-PSP solution on tomato and pepper plants significantly reduces the disease index of viral diseases,indicating that Rhp-PSP shows potential to develop antiviral agent in practice.

关键词： Rhodopseudomonas palustris Rhp-PSP tobacco mosaic virus endoribonuclease antiviral activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Accelerated water harvesting from vapor via a bio-inspired hydrogel pattern

引用

National Science Review 2024年第3期11卷 48-49页

作者： Lianbin zhang peng Wang State Key Laboratory of Material Processing and Die&Mould Technology Key Laboratory of Materials Chemistry for Energy Conversion and Storage of Ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology(HUST) School of Environmental Science and Engineering Sun Yat-sen University

Water and energy sustainability are integral parts of resource sustainability of the world. Due to water molecules' superior capability of serving as both hydrogenbond donor and acceptor, phase change of water, especially between liquid and vapor, underpins global water cycles,facilitates energy transformation and transfer, and thus helps shape the unique local weather and global climate patterns.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：