A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity

作     者：Lijie Chen Qianze Peng Xiaohua Du Weixing Zhang Ju’e Cheng Shu’e Sun Deyong Zhang Pin Su Yong Liu Lijie Chen;Qianze Peng;Xiaohua Du;Weixing Zhang;Ju'e Cheng;Shu'e Sun;Deyong Zhang;Pin Su;Yong Liu

作者机构：Longping BranchCollege of BiologyHunan UniversityChangsha 410082China State Key Laboratory of Hybrid Rice/Institute of Plant ProtectionHunan Academy of Agricultural SciencesChangsha 410125China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第6期

页      面：1967-1978页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 

基　　金：supported by the National Key R&D Program of China(2022YFD1400700) the Key Research and Development Program of Hunan Province,China(2022NK2014) the Hunan Natural Science Foundation,China(2022JJ40234) the Agricultural Science and Technology Innovation Fund Project of Hunan Province,China(2022CX1) the Changsha Natural Science Foundation,China(kq2202338) 

主　　题：Rhodopseudomonas palustris Rhp-PSP tobacco mosaic virus endoribonuclease antiviral activity 

摘      要：Plant virus causes massive crop losses ***,there is currently no effective measure to control plant viral ***,we identify an antiviral protein Rhp-PSP,produced by the bacterial Rhodopseudomonas palustris strain *** this study,we discover that the antiviral activity of Rhp-PSP relies on its endoribonuclease *** the arginine(R)residue at position 129 onto alanine(A)abolishs its endoribonuclease activity on coat protein(CP)RNA of tobacco mosaic virus(TMV),consequentially,compromises the antiviral activity of *** investigation demonstrates that,the mutant Rhp-PSP^(R129A)is unable to form the homotrimer as the wild type,indicating the importance of quaternary junction for the endoribonuclease *** of Rhp-PSP in Nicotiana benthamiana significantly enhances the resistance against TMV of seedlings,while expression of Rhp-PSP^(R129A)did not,confirming that endoribonuclease activity is responsible for the antiviral activity of *** addition,foliar spray of Rhp-PSP solution on tomato and pepper plants significantly reduces the disease index of viral diseases,indicating that Rhp-PSP shows potential to develop antiviral agent in practice.