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Effect of dietary supplement of inactivated Lactobacillus plantarum Ep-M 17 on growth performance,immune response,disease resistance,and intestinal microbiota in pen.eus vannamei

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Journal of Oceanology and Limnology 2024年第2期42卷 676-694页

作者： Ting WU Xiaoman HU Wenlong xu Yang DU Jiong CHEN State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingbo 315211China Laboratory of Biochemistry and Molecular Biology School of Marine SciencesNingbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology of Ministry of Education Ningbo UniversityNingbo 315211China

Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of pen.eus ***,high temperature and pressure during feed pelletizing is the main problem that can lead to a decrease in the activity of probiotics or cause their *** investigation needs to investigate whether inactivated Ep-M17 can exert similar effects as live ***,we evaluated the effects of inactivated *** Ep-M17 on growth performance,immune response,disease resistance,and gut microbiota in *** show that adding inactivated Ep-M17 to the feed also promoted body weight gain and increased relative immune protection in ***,histological examination revealed that the administration of inactivated Ep-M17 led to improvements in the density and distribution of microvilli in the intestines and enhancements in the abundance of B and R cells in the ***,the inactivated Ep-M17 supplementation resulted in increased activity levels of nutrient immune-related enzymes in both the shrimp hepatopancreas and ***,it stimulated the expression of Lvlec,pen.3a,Crustin,LGBP,Lysozyme,and proPo genes in both the hepatopancreas and ***,the inactivated Ep-M17 also increased bacterial diversity in the gut of shrimp and promoted the abundance of specific flora,facilitating the host organism’s metabolism and immunity to improve the disease resistance of ***,supplementation of inactivated *** Ep-M17 in shrimp diets can exert similar effects as live *** Ep-M17 effectively improving growth performance,gut microbiota,immune response,and disease resistance in ***.

关键词： pen.eus vannamei Lactobacillus plantarum Ep-M17 inactivated probiotics immune response gut flora

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Parameter Estimation of a Valve-Controlled Cylinder System Model Based on Bench Test and Operating Data Fusion

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Chinese Journal of Mechanical Engineering 2024年第2期37卷 247-263页

作者： Deying Su Shaojie Wang Haojing Lin Xiaosong Xia Yubing xu Liang Hou pen.Tung Sah Institute of Micro-Nano Science and Technology Xiamen UniversityXiamen 361102China xuzhou XCMG Excavation Machinery Co. LtdXuzhou 221000China

The accurate estimation of parameters is the premise for establishing a high-fidelity simulation model of a valve-controlled cylinder *** test data are easily obtained,but it is challenging to emulate actual loads in the research on parameter estimation of valve-controlled cylinder *** the actual load information contained in the operating data of the control valve,its acquisition remains *** paper proposes a method that fuses bench test and operating data for parameter estimation to address the aforementioned *** proposed method is based on Bayesian theory,and its core is a pool fusion of prior information from bench test and operating ***,a system model is established,and the parameters in the model are ***,the bench and operating data of the system are ***,the model parameters and weight coefficients are estimated using the data fusion ***,the estimated effects of the data fusion method,Bayesian method,and particle swarm optimisation(PSO)algorithm on system model parameters are *** research shows that the weight coefficient represents the contribution of different prior information to the parameter estimation *** effect of parameter estimation based on the data fusion method is better than that of the Bayesian method and the PSO *** load complexity leads to a decrease in model accuracy,highlighting the crucial role of the data fusion method in parameter estimation studies.

关键词： Valve-controlled cylinder system Parameter estimation The Bayesian theory Data fusion method Weight coefficients

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Arbitrary acoustic orbital angular momentum detection using dual-layer metasurfaces

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Science China(Physics,Mechanics & Astronomy) 2024年第6期67卷 106-112页

作者： Zhanlei Hao Haojie Chen Yuhang Yin Shan Zhu Yadong xu Cheng-Wei Qiu Huanyang Chen Department of Physics College of Physical Science and TechnologyXiamen UniversityXiamen 361005China Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore Department of Mechanical and Electrical Engineering Xiamen UniversityXiamen 361005China pen.Tung Sah Institute of Micro-Nano Science and Technology Xiamen UniversityXiamen 361005China School of Physical Science and Technology and Jiangsu Key Laboratory of Thin Films Soochow UniversitySuzhou 215006China

Orbital angular momentum(OAM),with its unique physical properties and vast application prospects,has attracted widespread attention in various ***,the development of valid and practical acoustic OAM detection methods continues to be a challenging *** this paper,we propose a novel construction method of dual-layer metasurfaces to achieve a doubleconversion process for the waveform reshaping and differentiated focusing of two-dimensional vortex sources with different ***,by utilizing a concise formula,a one-to-one correspondence is established between the OAM of incident vortex waves and different imaging *** fundamental principle of this special conversion relationship is rigorously constrained by the directional compen.ation of phase and the material parameters of dual-layer metasurfaces with different quadratic phase *** importantly,the highly consistent results between numerical demonstrations and acoustic experiments further confirm the feasibility and effectiveness of the proposed OAM detection *** work provides a new perspective on the precise manipulation for the phase of vortex fields,holding potential applications in super-resolution imaging and the design of acoustic OAM-based devices.

关键词： arbitrary acoustic OAM detection vortex waves dual-layer metasurfaces waveform reshaping differentiated focusing acoustic experiments

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Rapid and Sensitive Biosensing of Salmonella Using Mechanical Step Rotation and Gold@platinum Nanozymatic Amplification

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Journal of Analysis and Testing 2024年第3期8卷 262-269页

作者： Meixuan Li Lei Wang Ai xu Ying Ding Fengzhen Yang Yanbin Li Jianhan Lin Key Laboratory of Agricultural Information Acquisition Technology Ministry of Agriculture and Rural AffairsChina Agricultural University Center of Excellence for Poultry Science University of Arkansas

Inspired by ballpoint pen. an optical biosensor is developed for detection of Salmonellausing a mechanical step rotator with a dropper to greatly simplify operation, a high gradient magnetic field with immune magnetic nanobeads to specifically separate bacteria, and immune gold@platinum nanozymes with a smartphone to sensitively detect bacteria. First, the preloaded bacterial sample is drawn by the dropper to react with the immune magnetic nanobeads through repeated squeezing and releasing of the dropper, and the rotator is successively rotated and the dropper is repeatedly squeezed and released to achieve mixing of the bacteria, magnetic nanobeads and gold@platinum nanozymes, followed by magnetic separation to remove the background. Then, the conjugates are eff ectively washed with skim milk and ultrapure water, and the substrate are rapidly catalyzed into the product. Finally, the blue product is photographed and analyzed using a smartphone to obtain the Salmonella concentration. This biosensor could detect Salmonellaas low as 56 CFU/mL in～25 min.

关键词： gold@platinum nanozyme magnetic grid separation mechanical step rotator optical biosensor Salmonella detection

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Optical scanning endoscope via a single multimode optical fiber

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Opto-Electronic Science 2024年第3期3卷 1-32页

作者： Guangxing Wu Runze Zhu Yanqing Lu Minghui Hong Fei xu Department of Electrical and Computer Engineering National University of Singapore4 Engineering Drive 3117576Singapore College of Engineering and Applied Sciences and Collaborative Innovation Center of Advanced Microstructures Nanjing UniversityNanjing 210023China pen.Tung Sah Institute of Micro-Nano Science and Technology Xiamen University33 Qunxian RoadXiamen 361005China

Optical endoscopy has become an essential diagnostic and therapeutic approach in modern biomedicine for directly observing organs and tissues deep inside the human body,enabling non-invasive,rapid diagnosis and *** fiber endoscopy is highly competitive among various endoscopic imaging techniques due to its high flexibility,compact structure,excellent resolution,and resistance to electromagnetic *** the past decade,endoscopes based on a single multimode optical fiber(MMF)have attracted widespread research interest due to their potential to significantly reduce the footprint of optical fiber endoscopes and enhance imaging *** comparison with other imaging principles of MMF endoscopes,the scanning imaging method based on the wavefront shaping technique is highly developed and provides benefits including excellent imaging contrast,broad applicability to complex imaging scenarios,and good compatibility with various well-established scanning imaging *** this review,various technical routes to achieve light focusing through MMF and procedures to conduct the scanning imaging of MMF endoscopes are *** advancements in imaging performance enhancements,integrations of various imaging modalities with MMF scanning endoscopes,and applications are *** specific to this endoscopic imaging technology are analyzed,and potential remedies and avenues for future developments are discussed.

关键词： multimode optical fiber endoscope scanning imaging focusing wavefront shaping

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Determination of geocenter variations

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Chinese Science Bulletin 1999年第16期44卷 1517-1519页

作者： Bin Wu Bibo pen.Houze xu Chinese Acad Sci Inst Geodesy & Geophys Wuhan 430077 Peoples R China

The theory and methods to determine the geocenter variations by space geodetic techniques, especially by the satellite laser ranging have been discussed. Then the time series of geocenter variations have been derived from 11-year satellite laser ranging data to Lageos by the adopted methods. The results show that the standard deviations of monthly average

关键词： geocenter terrestrial reference frame satellite laser ranging.

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Convergence of Solutions of Discrete Reflected Backward SDE's and Simulations

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Acta Mathematicae Applicatae Sinica 2008年第1期24卷 1-18页

作者： Jean Mémin Shi-ge pen.Ming-yu xu Institute of Mathematics Shandong University Jinan 250100 China IRMAR Université de Rennesl Campus de Beaulieu Rennes 35042 France Depaxtment of Financial Mathematics and Control Science School of Mathematical Science Fudan University Shanghai 200433 China

The objective of this paper is to introduce elementary discrete reflected backward equations and to give a simple method to discretize in time a （continuous） reflected backward equation. A presentation of numerical ... 详细信息

关键词： Reflected equations backward equations weak convergence discretization discrete backward equations

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为提升IPTR患者血小板输注后CCI值建立分级规避HLA抗体对应抗原方法及HLAMatchmaker的应用研究

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中国实验血液学杂志 2024年第1期32卷 242-249页

作者：高素青徐筠娉罗畅如李大成彭龙刘通邹琼彩深圳市血液中心输血医学研究所深圳市第二人民医院血液科广东深圳518040

目的:建立分级规避HLA抗体MFI阈值对应抗原方法,联合应用HLAMatchmaker表位计算法,选择供患者表位最小错配评分值,评估两种方法为免疫性血小板输注无效(Immune platelet transfusion refractoriness,IPTR)患者选择HLA相容性血小板供者,... 详细信息

目的:建立分级规避HLA抗体MFI阈值对应抗原方法,联合应用HLAMatchmaker表位计算法,选择供患者表位最小错配评分值,评估两种方法为免疫性血小板输注无效(Immune platelet transfusion refractoriness,IPTR)患者选择HLA相容性血小板供者,在提升血小板输注后校正增加值(CCI)的应用价值。方法:采用SPRCA法完成51例IPTR患者的7807次血小板交叉配型实验,判断其免疫反应阴/阳性结果。采用Luminex单抗原流式微珠法检测患者的HLA-I类抗体,获得不同特异性抗体对应HLA-I类抗原MFI值,并将其分组及分级,强阳性组(MFI>4000,1级)、中阳性组(1000中阳性组>弱阳性组)。强阳性和中阳性组与阴性对照组之间的SPRCA实验免疫反应阳性结果检出数存在统计学差异(P0.05)。设置强阳性组为相应特异性HLA位点对应抗原1级规避阈值,中阳性组为2级规避阈值,弱阳性组为3级规避阈值,在供者血小板紧缺情况下,可以不需要规避弱阳性组。规避1和2级HLA-I类抗体对应供者抗原及选择HLAMatchmaker表位错配评分数≤7血小板供者策略,24 h内CCI值均>4.5×109/L,均可获得临床血小板输注有效。结论:在为IPTR患者选择HLA-I类相容性供者时,分级规避HLA-I类抗体对应供者抗原,综合选择供受者HLAMatchmaker表位错配评分数≤7,经血小板交叉配型实验确认为阴性结果的供者选择策略,对提升IPTR患者血小板计数具有一定实际应用价值。

关键词：血小板人类白细胞抗原抗体表位 HLAMatchmaker

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Innovative coaxial high-temperature thin-film sensor with core-shell structure surpassing traditional multilayer films

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Rare Metals 2024年第8期43卷 3854-3867页

作者： Chao Wu Guo-Chun Chen Fu-Xin Zhao Fan Lin Ying-Jun Zeng Yan-Zhang Fu Yan-Song Zhang Li-Da xu Qin-Nan Chen Rui Tang Dao-Heng Sun Zhen-Yin Hai pen.Tung Sah Institute of Micro-Nano Science and Technology Xiamen UniversityXiamen 361005China AECC Shenyang Engine Institute Shenyang 110015China School of Aerospace Engineering Xiamen UniversityXiamen 361005China

High-temperature thin-film sensors(TFSs)often suffer from inadequate tolerance to elevated *** this study,an innovative approach is presented to fabricate in situ integrated TFSs with a core-shell structure on alloy components using coaxial multi-ink printing *** method replaces traditional layerby-layer(LbL) deposition and LbL sintering processes and achieves simplified one-step *** coaxial TFS includes a conductive Pt core for conducting and sensing and a dielectric shell for electrical isolation and high-temperature *** coaxial Pt resistance grid demonstrates excellent high-temperature stability,with a resistance drift rate of only 0.08%·h^(-1) at 800 ℃,significantly lower than traditional Pt *** employing this method,a Pt thin-film strain gauge(TFSG) is fabricated that boasts remarkable high-temperature electromechanical *** effectively addresses the problem of sensitivity degradation experienced by traditional LbL Pt TFSGs when subjected to high *** demonstrate the system integration potential of the technique by printing and verifying the functionality of a long-path thinfilm resistance grid on turbine blades,which can withstand butane flame up to ~1300℃.These results showcase the potential of core-shell structure of the coaxial TFS for high-temperature applications,providing a novel approach to develop high-performance TFS beyond traditional multilayer structure.

关键词： Coaxial printing Core-shell structure Thinfilm strain gauge High-temperature stability

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有机凝胶肌电电极制备及其在动态手势识别的应用

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中南大学学报（自然科学版） 2024年第6期55卷 2153-2161页

作者：张建寰徐益鑫邓连钧徐周毅张陈涛厦门大学萨本栋微米纳米科学技术研究院福建厦门361102

为了解决传统肌电电极与皮肤贴合度不佳,易造成运动伪影的问题,本文提出一种高离子导电、良好自黏附的有机凝胶作为电极检测肌电信号,并将其应用于动态手势动作的识别。首先,采用物理交联与化学交联结合的互贯穿网络结构,在生物相容性... 详细信息

为了解决传统肌电电极与皮肤贴合度不佳,易造成运动伪影的问题,本文提出一种高离子导电、良好自黏附的有机凝胶作为电极检测肌电信号,并将其应用于动态手势动作的识别。首先,采用物理交联与化学交联结合的互贯穿网络结构,在生物相容性的聚丙烯酰胺/海藻酸钠体系中加入单宁酸、氯化钠和甘油,获得聚丙烯酰胺/海藻酸钠/单宁酸有机凝胶;其次,测试制备的有机凝胶力学、导电及黏附等性能,并将其作为皮肤表面电极进行肌电信号的检测;最后,使用卷积神经网络算法将其应用于动态手势动作的识别。研究结果表明:制备的有机凝胶具有低弹性模量以及可逆自黏附能力,可实现与皮肤的保形黏附;有机凝胶具有7.7 kPa的弹性模量、310%的伸长率、2.81 mS/cm的离子导电率,并具有良好的自黏附性能以及可降解性,能够获得17.14 dB信噪比的高质量肌电信号,能够有效识别不同界面操作的动态动作及手语手势,平均识别率分别达95.04%和98.67%。该有机凝胶电极具有高信噪比,可有效识别动态手势,在人机交互、机器人遥操作领域具有广阔的应用前景。

关键词：肌电信号有机凝胶柔性电极动态手势识别

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