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Zika virus and Zika fever

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Virologica Sinica 2016年第2期31卷 103-109页

作者： Zhaoyang wang peigang wang Jing An Department of Microbiology School of Basic Medical Sciences Chinese Capital Medical University

An emerging mosquito-borne arbovirus named Zika virus(ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild symptoms. However, recently, with its rapid spread from Asia to the Americas, affecting more than 30 countries, accumulating evidences have demonstrated a close association between infant microcephaly and Zika infection in pregnant women. Here, we reviewed the virological, epidemiological, and clinical essentials of ZIKV infection.

关键词：虫媒病毒出血病毒感染黄病毒科流行病学散发性病毒学畸形

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切开复位内固定治疗锁骨双极脱位四例

中国修复重建外科杂志 2024年第2期38卷 151-154页

作者：高培刚王兴存鲍红刚中国人民解放军中部战区空军医院骨科山西大同037006

目的总结切开复位内固定治疗4例锁骨双极脱位的方法及疗效。方法2017年6月—2022年6月,收治4例锁骨双极脱位患者。男3例,女1例;年龄27~63岁,平均45岁。致伤原因:矿车挤压伤2例,交通事故伤1例,重物砸伤1例。受伤至手术时间为3~7 d,平均5.... 详细信息

目的总结切开复位内固定治疗4例锁骨双极脱位的方法及疗效。方法2017年6月—2022年6月,收治4例锁骨双极脱位患者。男3例,女1例;年龄27~63岁,平均45岁。致伤原因:矿车挤压伤2例,交通事故伤1例,重物砸伤1例。受伤至手术时间为3~7 d,平均5.0 d。胸锁关节脱位分型:GradeⅡ型1例,Ⅲ型3例;前脱位3例,后脱位1例。肩锁关节脱位分型:TossyⅡ型2例,Ⅲ型2例。均行切开复位内固定术,胸锁关节、肩锁关节分别采用外踝锁定钛板、锁骨钩钢板固定。结果4例手术均顺利完成,无血管、神经损伤;术后切口均Ⅰ期愈合。患者均获随访,随访时间12~18个月,平均14个月。末次随访时,肩关节功能根据Rockwood评分法评定,获优3例、良1例。随访期间无内固定物松动、断裂发生。4例均于术后5~7个月二次手术取出内固定物,平均6个月;取出后无关节再脱位发生。结论锁骨双极脱位切开复位后,以外踝锁定钛板固定胸锁关节、锁骨钩钢板固定肩锁关节,具有手术操作简便、安全性高、固定牢固、并发症少等优势,患者肩部功能恢复较好。

关键词：锁骨双极脱位胸锁关节肩锁关节切开复位内固定

Prion Protein Expression is Correlated with Glioma Grades

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Virologica Sinica 2020年第4期35卷 490-493页

作者： Qiaoli Luo Yisong wang Dongying Fan Shijie wang peigang wang Jing An Department of Microbiology School of Basic Medical SciencesCapital Medical UniversityBeijing 100069China Center of Epilepsy Beijing Institute for Brain DisordersBeijing 100093China

Dear Editor,Glioma is the most common primary central nervous system(CNS)tumors in human.Gliomas are classified into low-grade glioma(LGG,grades I–II)and high-grade glioma(HGG,grades III–IV)according to the World Health Organization classification guidelines(Louis et al.2007).Comparing to the LGG,the HGG becomes more aggressive and has a poorer median overall survival.At present,glioma is mainly treated by radiotherapy,chemotherapy and surgical resection,but the effect is not ideal,especially for the glioblastoma(GBM),which is the most malignant type of gliomas and is resistant to current therapy protocols.There are several theories about the pathogenesis of glioma,such as radiation(Ohgaki and Kleihues 2005)。

关键词： glioma al. Glioma

Electroporation-Mediated Immunization of a Candidate DNA Vaccine Expressing Dengue Virus Serotype 4 prM-E Antigen Confers Long-Term Protection in Mice

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Virologica Sinica 2019年第1期34卷 88-96页

作者： Ziyang Sheng Hui Chen Kaihao Feng Na Gao Ran wang peigang wang Dongying Fan Jing An Department of Microbiology School of Basic Medical SciencesCapital Medical University Center of Epilepsy Beijing Institute for Brain Disorders

Dengue fever, caused by dengue viruses(DENVs), is a widespread mosquito-borne zoonotic disease; however, there is no available anti-dengue vaccine for worldwide use. In the current study, a DNA vaccine candidate(pV-D4 ME) expressing prM-E protein of DENV serotype 4(DENV-4) was constructed, and its immunogenicity and protection were evaluated in immunocompetent BALB/c mice. The pV-D4 ME candidate vaccine induced effective humoral and cellular immunity of mice against DENV-4 in vivo when administered both at 50 μg and 5 μg through electroporation. Two weeks after receiving three immunizations, both doses of pV-D4 ME DNA were shown to confer effective protection against lethal DENV-4 challenge. Notably, at 6 months after the three immunizations, 50 μg, but not 5 μg, of pV-D4 ME could provide stable protection(100% survival rate) against DENV-4 lethal challenge without any obvious clinical signs. These results suggest that immunization with 50 μg pV-D4 ME through electroporation could confer effective and long-term protection against DENV-4, offering a promising approach for development of a novel DNA vaccine against DENVs.

关键词： Dengue virus (DENV) DNA vaccine Long-term protection Electroporation Mouse

Decreases in Both the Seroprevalence of Serum Antibodies and Seroprotection against Japanese Encephalitis Virus among Vaccinated Children

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Virologica Sinica 2019年第3期34卷 243-252页

作者： Ran wang Lyu Xie Na Gao Dongying Fan Hui Chen peigang wang Hongning Zhou Jing An Department of Microbiology School of Basic Medical Sciences. Chinese Capital Medical UniversityBeijing 100069China Yunnan Provincial Key Laboratory of Vector-borne Disease Control and Research Yunnan Institute of Parasitic DiseasesPu'er 665000China Center of Epilepsy Beijing Institute for Brain DisordersBeijing 100069China

The incidence of Japanese encephalitis(JE)has significantly decreased in China due to JE vaccines.In this study,we investigated the post-JE vaccination seroprevalence and protection provided by vaccinated sera against Japanese encephalitis virus(JEV)to elucidate the persistence and waning of antibodies to JEV among JE-SA14-14-2-vaccinated children.A total of 300 serum samples were collected from vaccinated children aged 3-10 years in Zhaotong,Yunnan,China.The seroprevalence of anti-JEV antibodies was determined by enzyme-linked immune sorbent assay and plaque reduction neutralization test.The highest seropositivity of 82%was observed in vaccinated children during the first 0.5-1.5 years after booster vaccination.Then,the seropositivity began to decline and remained lower than the original level observed in the 0.5-1.5-year group.An association was found between the waning of seroprevalence and elapsed time of the post-booster vaccination.Similarly,the neutralizing antibody(nAb)titres gradually decreased over time,and the levels showed a positive correlation with the protective efficacy in mice.This finding suggests that nAbs play an important role in the antiviral process and that the nAb titre is an adequately credible parameter for evaluating the protective efficacy induced by the JE vaccine.Our results provide data that clarify the persistence and waning of antibodies to JEV,which may help elucidate the pathogenesis of JE.

关键词： Japanese encephalitis virus(JEV) SA14-14-2 Seroprevalence Neutralizing antibodies Waning of antibody

Study on wellbore temperature of riserless mud recovery system by CFD approach and numerical calculation

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Petroleum 2020年第2期6卷 163-169页

作者： Xin Li Jie Zhang Xu Tang Gezhen Mao peigang wang State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengduChina

The riserless mud recovery(RMR)system abandons the riser used in conventional offshore drilling,and the drill string above the seabed is directly exposed to seawater,resulting in convective heat transfer from the drilling fluid in the drill string to seawater.Therefore,the wellbore temperature distribution in the RMR system is quite different from the conventional offshore drilling.In this paper,based on the heat transfer characteristics of the RMR system,a mathematical model of the thermal field of the RMR system is established.The data used in this paper come from a vertical well in the South China Sea.Computational Fluid Dynamics(CFD)software is used to simulate the temperature distribution in drill string at different seawater depths and different formation depths in this paper,and the simulation results are compared with the calculation results of the mathematical model,so as to verify the feasibility of the mathematical model established in this paper.Combined with the calculation results of the mathematical model,this paper also explores the effect of different discharge capacity and different injection temperature of drilling fluid on the wellbore temperature change.

关键词： RMR Wellbore temperature Thermal field Mathematical model CFD

Elevated expression of EBV and TLRs in the brain is associated with Rasmussen's encephalitis

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Virologica Sinica 2017年第5期32卷 423-430页

作者： Xin wang Yisong wang Dong Liu peigang wang Dongying Fan Yuguang Guan Tianfu Li Guoming Luan Jing An Department of Microbiology School of Basic Medical Sciences Capital Medical University Beijing 100069 China Department of Neurosurgery Sanbo Brain Hospital Capital Medical University Beijing 100093 China Beijing Key Laboratory of Epilepsy Beijing 100093 China Center of Epilepsy Beijing Institute for Brain Disorders Beijing 100093 China

Rasmussen's encephalitis(RE) is a rare pediatric neurological disorder, the etiology of which remains unclear. It has been speculated that the immunopathogenesis of RE involves damage to neurons, which eventually leads to the occurrence of RE. Viral infection may be a critical factor in triggering RE immunopathogenesis. In this study, we analyzed the expression of Epstein-Barr virus(EBV) antigens as well as of Toll-like receptor 3(TLR3), TLR9, and downstream adapter TIRdomain-containing adapter-inducing interferon-β(TRIF) in the brain tissues of 26 patients with RE and 16 control individuals using immunohistochemistry(IHC). In the RE group, EBV antigens were detected in 53% of individuals at various expression levels. In contrast, there was no detectable EBV antigen expression in control brain tissues. Moreover, we found marked increases in the expression of TLR3, TLR9, and TRIF in the brain tissues of RE patients compared with levels in the control group. Furthermore, among RE cases, EBV expression and high TLR3 expression were associated with more severe brain atrophy. Our results suggest that the elevated expression of EBV and TLRs may be involved in RE occurrence through the activation of downstream molecules.

关键词： Rasmussen’s encephalitis(RE) Epstein-Barr virus(EBV) Toll-Like receptor(TLR) TIR-domain-containing adapter-inducing interferon-β(TRIF) epilepsy

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen's Encephalitis

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Virologica Sinica 2018年第5期33卷 402-409页

作者： Dong Liu Xin wang Yisong wang peigang wang Dongying Fan Sichang Chen Yuguang Guan Tianfu Li Jing An Guoming Luan department of neurosurgery sanbo brain hospitalcapital medical universityBeijing 100093China department of microbiology school of basic medical sciencecapital medical universityBeijing 100069China department of neurosurgery xuanwu hospital of capital medical universityBeijing 100053China beijing key laboratory of epilepsy center of epilepsy beijing institute for brain disordersBeijing 100093China

Rasmussen's encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis,intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8?T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8?T cells are involved in the pathogenesis of RE.

关键词： Rasmussen’s encephalitis (RE) Epstein-Barr virus (EBV) Human herpes virus 6 (HHV6) Seizures CD8^+T cells

边缘海盆地断层差异演化成因的数值模拟:以西湖凹陷平北斜坡带为例

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海洋地质与第四纪地质 2024年第1期44卷 81-95页

作者：马皓然苏金宝王毛毛任培罡谈明轩河海大学海洋学院南京210098 中石化华东石油工程有限公司科技发展分公司南京210098

东海陆架盆地中生代以来的成盆过程中,发育了大量陆倾控盆断裂,其发育模式与形成演化与东亚其他边缘海盆地差异明显。前人关注东海陆架盆地迁移特征,而忽略了断层差异演化的形成机制,对断层发育过程控制因素缺少深入研究。西湖凹陷平北... 详细信息

东海陆架盆地中生代以来的成盆过程中,发育了大量陆倾控盆断裂,其发育模式与形成演化与东亚其他边缘海盆地差异明显。前人关注东海陆架盆地迁移特征,而忽略了断层差异演化的形成机制,对断层发育过程控制因素缺少深入研究。西湖凹陷平北斜坡带北部为海倾断层组成的断阶区,南部为陆倾断层组成的半地堑区,断裂差异演化指示着东海陆架盆地的成盆过程。本文通过离散元数值模拟,模拟陆倾和海倾断层的形成及演化,以探讨断层几何发育特征的控制因素。结果表明,岩性差异对斜坡带断层演化有较大影响,较高抗剪强度岩层破裂易产生陆倾控盆断裂,而低抗剪强度岩石则易形成向海倾断层。应力作用方向是区域差异演化的重要控制因素,岩石强度相同,应力作用方向相反时,断层倾向相反。盆地形成过程中发育众多凹陷斜坡,但坡度不是断层差异演化的主导因素。平北斜坡带和边缘海盆地的差异演化可能是由基底强度差异或应力方向差异导致的。本文利用离散元数值模拟平北斜坡带断裂差异演化过程,为东海盆地构造演化机制及边缘海盆地的形成提供了思路和理论依据。

关键词：断层差异演化离散元模拟边缘海盆地西湖凹陷平北斜坡带