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Bacillus licheniformis PF9 improves barrier function and alleviates inflammatory responses against enterotoxigenic Escherichia coli F4 infection in the porcine intestinal epithelial cells

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1413-1427页

作者： Qiao Li Linyan Li Yanhong chen Changning Yu paula Azevedo Joshua Gong chengbo Yang College of Veterinary Medicine Nanjing Agricultural UniversityNanjing 210095China Department of Animal Science University of ManitobaWinnipegManitoba R3T 2N2Canada Guelph Research and Development Centre Agriculture Agri-Food CanadaGuelphOntario N1G 5C9Canada State Key Laboratory of Food Science and Technology Nanchang UniversityNanchang 330047China

Background:Enterotoxigenic Escherichia coli(ETEC)F4 commonly colonizes the small intestine and releases enterotoxins that impair the intestinal barrier function and trigger inflammatory *** Bacillus licheniformis(***)has been reported to enhance intestinal health,it remains to be seen whether there is a functional role of *** in intestinal inflammatory response in intestinal porcine epithelial cell line(IPEC-J2)when stimulated with ETEC ***:In the present study,the effects of *** PF9 on the release of pro-inflammation cytokines,cell integrity and nuclear factor-κB(NF-κB)activation were evaluated in ETEC F4-induced IPEC-J2 ***:*** PF9 treatment was capable of remarkably attenuating the expression levels of inflammation cytokines tumor necrosis factor-α(TNF-α),interleukin(IL)-8,and IL-6 during ETEC F4 ***,the gene expression of Toll-like receptor 4(TLR4)-mediated upstream related genes of NF-κB signaling pathway has been significantly *** changes were accompanied by significantly decreased phosphorylation of p65 NF-κB during ETEC F4 infection with *** PF9 *** immunofluorescence and western blotting analysis revealed that *** PF9 increased the expression levels of zona occludens 1(ZO-1)and occludin(OCLN)in ETEC F4-infected IPEC-J2 ***,the *** PF9 could alleviate the injury of epithelial barrier function assessed by the trans-epithelial electrical resistance(TEER)and cell permeability ***,*** PF9 protect IPEC-J2 cells against ETEC F4 infection by decreasing the gene expressions of virulence-related factors(including luxS,estA,estB,and elt)in ETEC ***:Collectively,our results suggest that *** PF9 might reduce inflammation-related cytokines through blocking the NF-κB signaling ***,*** PF9 displayed a significant role in the enhancem

关键词： Bacillus licheniformis PF9 Barrier function ETEC F4 Inflammatory response IPEC-J2

Characterization of glutamatergic VTA neural population responses to aversive and rewarding conditioning in freely-moving mice

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Science Bulletin 2019年第16期64卷 1167-1178页

作者： Quentin Montardy Zheng Zhou Zhuogui Lei Xuemei Liu Pengyu Zeng chen chen Yuanming Liu paula Sanz-Leon Kang Huang Liping Wang Shenzhen Key Lab of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science Guangdong Provincial Key Laboratory of Brain Connectome and BehaviorCAS Center for Excellence in Brain Science and Intelligence TechnologyThe Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsShenzhen 518055China University of Chinese Academy of Sciences Beijing 100049China School of Physics the University of SydneySydney 2006Australia Centre for Integrative Brain Function the University of SydneySydney 2006Australia Department of Biomedical Sciences City University of Hong KongTat Chee AvenueKowloonHong KongSAR 999077China

The Ventral Tegmental Area(VTA) is a midbrain structure known to integrate aversive and rewarding stimuli, but little is known about the role of VTA glutamatergic(VGluT2) neurons in these *** activation of VGluT2 soma evokes rewarding behaviors, while activation of their downstream projections evokes aversive behaviors. To facilitate our understanding of these conflicting properties, we recorded calcium signals from VTAVGluT2+ neurons using fiber photometry in VGluT2-cre mice to investigate how this population was recruited by aversive and rewarding stimulation, both during unconditioned and conditioned protocols. Our results revealed that, as a population, VTAVGluT2+neurons responded similarly to unconditioned-aversive and unconditioned-rewarding stimulation. During aversive and rewarding conditioning, the CS-evoked responses gradually increased across trials whilst the US-evoked response remained stable. Retrieval 24 h after conditioning, during which mice received only CS presentation, resulted in VTAVGluT2+ neurons strongly responding to CS presentation and to the expected-US but only for aversive conditioning. To help understand these differences based on VTAVGluT2+ neuronal networks, the inputs and outputs of VTAVGluT2+ neurons were investigated using Cholera Toxin B(CTB) and rabies virus. Based on our results, we propose that the divergent VTAVGluT2+ neuronal responses to aversion and reward conditioning may be partly due to the existence of VTAVGluT2+ subpopulations that are characterized by their connectivity.

关键词： Ventral Tegmental Area Aversion Reward Conditioning Behavior

Mice Heat Chamber Calibration

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Journal of Analytical Sciences, Methods and Instrumentation 2014年第4期4卷 95-101页

作者： paula Y. S. Poh Samuel Carus Yifan chen Patricia A. Deuster Department of Military and Emergency Medicine Uniformed Services University of the Health Sciences Bethesda MD USA University of Illinois at Urbana-Champaign Urbana-Champaign IL USA

Background: Firm conclusions regarding the etiology of heat exposure responses among animals are difficult to draw due to different experimental designs and methodological confounders, such as environmental chamber set-up and heating rates. The purpose of this investigation was to 1) validate the heat test protocol for mice models via cage location and orientation;and to 2) determine the maximum number of cages that can be used without compromising individual heating rates. Methods: A mice temperature transponder (G2 E-Mitter, Mini Meter, Respironics) was centrally positioned inside each mice cage set in the environmental chamber (Thermo Scientific Forma, Model 3961). Two cage orientations (adjacent, left-to-right and parallel, front-to-back) with 3 set-ups (top shelf, bottom shelf and both shelves) using 2 and 4 cages were examined in triplicate and averaged. Transponders equilibrated at 21.5℃ for 5 min, then exposed to 39.5℃ for a minimum of 60 min. Results: A major finding was that adjacent (L-R) top shelf set-up had the smallest temperature difference throughout the heat test (Δ = 0.43℃ vs. Δ = 2.2℃) and at minute 60 (Δ = 0.2℃ vs. Δ = 1.8℃). Both orientations for the bottom shelf set-up had a slower rise in temperature (0.04℃·min-1) than other set-ups (0.3℃·min-1). Using both shelves, top shelf cages were consistently warmer than bottom shelf cages (1.0℃ - 3.6℃) for both orientations. Conclusions: We strongly suggest using an adjacent (L-R) top shelf set-up since it enabled uniform chamber heating rates and standardized heat exposure. Bottom shelf is not recommended for use due to poor heating rate performance. Since an increased number of cages may obstruct heat flow patterns, a one shelf set-up with 2 cages should be used.

关键词： Heat Stress Core Temperature Mice Model

Modelling and Optimization of Clean and Affordable Electricity Solution for Small-Scale Savings and Credit Cooperatives(SACCOs)

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Energy Engineering 2023年第4期120卷 791-810页

作者： Xiaomei Lin Samuel Bimenyimana chenWang Godwin Norense Osarumwensa Asemota Jeanne paula Ihirwe Aphrodis Nduwamungu Etienne Ntagwirumugara Athanase Gace Changfu Yuan Yaowen chen Changtai Li Shilpi Bora YiYi Mo Jiyu Lai Mabao Wu Yong Tang Merit M.Huang College of Science and Technology The Open University of FujianFuzhou350013China Intelligence and Automation in Construction Provincial Higher–Educational Engineering Research Centre Huaqiao UniversityXiamen361021China African Centre of Excellence in Energy for Sustainable Development University of RwandaKigali4285Rwanda Morayo College Nairobi999070Kenya Department of Economics and Management Hebei University of TechnologyTianjin300401China College of Science and Technology University of RwandaKigali4285Rwanda Fujian Baichuan Construction Technology Co. Ltd.HuliXiamen361021China Haoqi(Xiamen)Construction Technology Co. Ltd.HaicangXiamen361021China School ofDesign Ajeenkiya D Y PatilUniversityPune412105India College of Civil Engineering Huaqiao UniversityXiamen361021China College of Transportation and Civil Engineering Fujian Agriculture and Forestry UniversityFuzhou350100China Highway Southeast Engineering Co. Ltd.Xiamen361100China Fuzhou Sinotom Information Technology Co. Ltd.Fuzhou350001China

Renewable energy exploitation is among the development strategies set by the government of Rwanda on the roadmap to 2023/2024 universal electricity access and theUnitedNations plans by *** previous studies oncleanenergy technologies inRwandahavemostly focusedonhouseholds’usagebut there are currentlynostudies describing the feasibility of clean energy technologies for financial *** skepticism on renewable energy in Africa was previously reported by some *** realized that most SACCOs(Savings and Credit Co-Operatives)in Rwanda use diesel technology for backup/emergency electricity supply,taking consideration of abundant solar resources in Rwanda,having seen the viability and feasibility studies from other countries of renewable energy for different institutions(financial included);thiswork uses theHOMEREnergy Software and the electricity load profile of a typical SACCO in Rwanda to analyse the affordability and viability of on-site renewable energy generation for SACCO in *** results reveal that a solar PV systemwith storage can be the optimal solution(with levelized cost of electricity(LCOE)of 0.713$/kWh which is cheaper than 0.73$/kWh for diesel technology)for SACCOs located in both off-grid areas and grid-connected areas(with 0.041$/kWh LCOE which is lower than the current electricity tariff in Rwanda).The findings in this work can serve as basic tools/materials for policy drafters in Rwanda on how financial institutions can contribute to climate change mitigation through self-renewable energy exploitation.

关键词： HOMER energy solar energy SACCO in Rwanda climate change mitigation

小麦TILLING突变体检测中基因组DNA提取方法的优化

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麦类作物学报 2017年第2期37卷 185-191页

作者：徐艳徐继法陈磊赵吉强 FU Zeyu JAMESON paula E 宋建成烟台大学生命科学学院山东烟台264005 School of Biological Sciences University of Canterbury

为了优化小麦高通量TILLING突变体扫描所需基因组DNA提取的方法,对利用SDS法、改良SDS法、CTAB法、改良CTAB法、AxyPrep DNA提取试剂盒、Qiagen DNeasy Plant Mini Kit试剂盒以及再生Qiagen硅胶柱方法提取的小麦基因组DNA进行了系统比... 详细信息

为了优化小麦高通量TILLING突变体扫描所需基因组DNA提取的方法,对利用SDS法、改良SDS法、CTAB法、改良CTAB法、AxyPrep DNA提取试剂盒、Qiagen DNeasy Plant Mini Kit试剂盒以及再生Qiagen硅胶柱方法提取的小麦基因组DNA进行了系统比较。结果表明,尽管7种方法所提取DNA的纯度及琼脂糖凝胶电泳检测结果均良好,但再生Qiagen硅胶柱方法、CTAB法和改良SDS法的DNA浓度显著高于其他方法。根据小麦细胞分裂素氧化酶基因1(TaCKX1)的DNA序列设计引物,以新鲜DNA样品用于PCR扩增大于1 000bp的片段时,后4种方法扩增效果优于前3种方法;DNA在-20℃下保存100d后重新进行PCR扩增时,则只有Qiagen试剂盒和再生Qiagen硅胶柱2种方法提取的DNA能扩增出大于1 000bp的片段。采用本实验室与新西兰坎特伯雷大学合作建立的再生硅胶柱与自配提取液提取小麦基因组DNA,既保证了DNA的高质量,又降低了使用试剂盒的成本,为小麦TILLING库的构建及其他相关试验提供了一种经济有效的DNA提取方法。

关键词：小麦 TILLING 基因组DNA 提取方法

Preserving cognitive function in patients with Alzheimer's disease:The Alzheimer's disease neuroprotection research initiative(ADNRI)

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Neuroprotection 2023年第2期1卷 84-98页

作者： Jie Liu Heleen van Beusekom Xian-Le Bu Gong chen Paulo Henrique Rosado de Castro Xiaochun chen Xiaowei chen Andrew N.Clarkson Tracy D.Farr Yuhong Fu Jianping Jia Jukka Jolkkonen Woojin Scott Kim paula Korhonen Shen Li Yajie Liang Guang-Hui Liu Guiyou Liu Yu-Hui Liu Tarja Malm Xiaobo Mao Joaquim Miguel Oliveira Mike M.Modo Pedro Ramos-Cabrer Karsten Ruscher Weihong Song Jun Wang Xuanyue Wang Yun Wang Haitao Wu Lize Xiong Yi Yang Keqiang Ye Jin-Tai Yu Xin-Fu Zhou Marietta Zille Colin L.Masters Piotr Walczak Boltze Johannes Xunming Ji Yan-Jiang Wang Department of Neurology Daping HospitalThird Military Medical UniversityChongqingChina Chongqing Key Laboratory of Ageing and Brain Diseases ChongqingChina Division of Experimental Cardiology Department of CardiologyErasmus MCUniversity Medical CenterRotterdamThe Netherlands Institute of Brain and Intelligence Third Military Medical UniversityChongqingChina Guangdong-HongKong-Macao Institute of CNS Regeneration(GHMICR) Jinan UniversityGuangzhouGuangdongChina Institute of Biomedical Sciences Federal University of Rio de JaneiroRio de JaneiroRio de JaneiroBrazil Fujian Key Laboratory of Molecular Neurology Department of Neurology and GeriatricsFujian Institute of GeriatricsFujian Medical University Union HospitalInstitute of NeuroscienceFujian Medical UniversityFuzhouFujianChina Guangyang Bay Laboratory Chongqing Institute for Brain and IntelligenceChongqingChina Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghaiChina Department of Anatomy Brain Health Research Centre and Brain Research New ZealandUniversity of OtagoDunedinNew Zealand School of Life Sciences University of NottinghamNottinghamUK Brain and Mind Centre&School of Medical Sciences The University of SydneySydneyNew South WalesAustralia Innovation Center for Neurological Disorders and Department of Neurology Xuanwu HospitalNational Clinical Research Center for Geriatric DiseasesCapital Medical UniversityBeijingChina A.I.Virtanen Institute for Molecular Sciences University of Eastern FinlandKuopioFinland Department of Neurology and Psychiatry Beijing Shijitan HospitalCapital Medical UniversityBeijingChina Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of MedicineBaltimoreMarylandUSA University of Chinese Academy of Sciences BeijingChina State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute of Brain Disorders Laboratory of Brain DisordersMinistry of Sci

The global trend toward aging populations has resulted in an increase in the occurrence of Alzheimer's disease(AD)and associated socioeconomic *** metabolism of amyloid-β(Aβ)has been proposed as a significant pathomechanism in AD,supported by results of recent clinical trials using anti-Aβ***,the cognitive benefits of the current treatments are *** etiology of AD is multifactorial,encompassing Aβand tau accumulation,neuroinflammation,demyelination,vascular dysfunction,and comorbidities,which collectively lead to widespread neurodegeneration in the brain and cognitive ***,solely removing Aβfrom the brain may be insufficient to combat neurodegeneration and preserve *** attain effective treatment for AD,it is necessary to(1)conduct extensive research on various mechanisms that cause neurodegeneration,including advances in neuroimaging techniques for earlier detection and a more precise characterization of molecular events at scales ranging from cellular to the full system level;(2)identify neuroprotective intervention targets against different neurodegeneration mechanisms;and(3)discover novel and optimal combinations of neuroprotective intervention strategies to maintain cognitive function in AD *** Alzheimer's Disease Neuroprotection Research Initiative's objective is to facilitate coordinated,multidisciplinary efforts to develop systemic neuroprotective strategies to combat *** aim is to achieve mitigation of the full spectrum of pathological processes underlying AD,with the goal of halting or even reversing cognitive decline.

关键词： Alzheimer's disease early intervention neural regeneration neuroprotection systematic perspective

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闽宁对口帮扶的经济理论创新

中国经济问题 2022年第5期 1-11页

作者：周颖刚陈煌厦门大学经济学院与王亚南经济研究院厦门大学经济学院与邹至庄经济研究院

本文以习近平经济思想为指导,把马克思主义基本原理同中国具体实际相结合、同中华优秀传统文化相结合,从理论上对闽宁对口帮扶进行提炼并将其中的经济理论创新归纳为:立足“有爱社会人”假设,创造性地在国家治理中加入合理的减贫激励机... 详细信息

本文以习近平经济思想为指导,把马克思主义基本原理同中国具体实际相结合、同中华优秀传统文化相结合,从理论上对闽宁对口帮扶进行提炼并将其中的经济理论创新归纳为:立足“有爱社会人”假设,创造性地在国家治理中加入合理的减贫激励机制设计,鼓励和发扬集体主义、利他主义,营造“有爱社会”氛围;在“有为政府”的引导下充分发挥市场在资源配置中的决定性作用,将各项政策制度化以促进“有效市场”的长期减贫效应,最终实现共同富裕。

关键词：习近平经济思想闽宁对口帮扶有爱社会有为政府有效市场

基于GTAP模型的中缅印度洋新通道贸易效应研究

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云南财经大学学报 2024年第10期40卷 89-110页

作者：胡颖陈洁筠云南大学经济学院昆明650504 厦门大学邹至庄经济研究院厦门361000

中国-缅甸-印度洋海公铁联运新通道,是从中国西南地区打通印度洋出海通道的必经之路,其发展与“一带一路”孟中印缅经济走廊建设和中国西部沿边开放紧密关联。从贸易网络、贸易依赖度、竞争性和互补性等方面探究通道开通后沿线国家和地... 详细信息

中国-缅甸-印度洋海公铁联运新通道,是从中国西南地区打通印度洋出海通道的必经之路,其发展与“一带一路”孟中印缅经济走廊建设和中国西部沿边开放紧密关联。从贸易网络、贸易依赖度、竞争性和互补性等方面探究通道开通后沿线国家和地区间贸易规模、贸易结构和贸易壁垒的变动,揭示了沿线国家和地区贸易关系的特征和动态变化。根据运输成本对贸易的传导机制,采用GTAP模型,模拟预测中缅印度洋新通道在部分联通、全线贯通和路网形成三种不同情景下产生的贸易效应。研究发现:中缅印度洋新通道的开通将直接刺激沿线地区贸易规模增长,贸易条件显著改善,中国、缅甸和印度等国实际GDP和社会福利水平显著提升;随着物流成本下降和贸易网络深化,新通道促进贸易从高成本的域外地区向低成本的域内地区转移,重构区内贸易格局;新通道的开通将有利于推动中国与周边国家和地区形成产业链互补,促进区域经济一体化发展。

关键词：中缅印度洋新通道贸易效应全球贸易分析(GTAP)模型

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沃格尔戏剧《狂热与悸动》中的叙事

当代外国文学 2021年第2期42卷 46-52页

作者：张巧珠陈爱敏南京师范大学外国语学院

美国剧作家保拉·沃格尔的剧作《狂热与悸动》融多种叙事手法为一体,是颇具革新性和后现代主义特征之作。本文从非自然叙事学视角入手,对剧中的叙事手法与策略进行考察。论文认为:"剧内音"与"剧外音"的使用,架起了观演双方间的桥梁,帮... 详细信息

美国剧作家保拉·沃格尔的剧作《狂热与悸动》融多种叙事手法为一体,是颇具革新性和后现代主义特征之作。本文从非自然叙事学视角入手,对剧中的叙事手法与策略进行考察。论文认为:"剧内音"与"剧外音"的使用,架起了观演双方间的桥梁,帮助观众了解剧情,推动故事发展。普通舞台灯光和蓝色舞台灯光塑造的两个横向平行空间叙事,外化了人物内心世界,揭露了单身女性的生存状态。纵向、非线性、碎片化的意识流空间叙事,则展露了女主人公的意识流动,凸显了不同角色的追求与欲望。通过非自然叙事,剧作者批评了当下美国社会的家庭暴力与失衡的男女关系。

关键词：保拉·沃格尔《狂热与悸动》非自然叙事