Characterization of glutamatergic VTA neural population responses to aversive and rewarding conditioning in freely-moving mice

作     者：Quentin Montardy Zheng Zhou Zhuogui Lei Xuemei Liu Pengyu Zeng Chen Chen Yuanming Liu Paula Sanz-Leon Kang Huang Liping Wang 

作者机构：Shenzhen Key Lab of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain ScienceGuangdong Provincial Key Laboratory of Brain Connectome and BehaviorCAS Center for Excellence in Brain Science and Intelligence TechnologyThe Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsShenzhen 518055China University of Chinese Academy of SciencesBeijing 100049China School of Physicsthe University of SydneySydney 2006Australia Centre for Integrative Brain Functionthe University of SydneySydney 2006Australia Department of Biomedical SciencesCity University of Hong KongTat Chee AvenueKowloonHong KongSAR 999077China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2019年第64卷第16期

页      面：1167-1178页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：supported by the National Natural Science Foundation of China, China (31630031, 81425010, 31471109, 31671116, and 31500861) International Partnership Program of Chinese Academy of Sciences, China (172644KYS820170004) Helmholtz-CAS Joint Research Grant (GJHZ1508) Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, China (2017B030301017) Shenzhen Governmental Grants, China (JCYJ20160429190927063, KQJSCX20160301144002, JCYJ20170413164535041, JCYJ20150 401150223647, JCYJ20160429185854999, JSGG2016042919052 1240) Research Instrument Development Project of the Chinese Academy of Sciences, China (YJKYYQ20170064) Youth Innovation Promotion Association of Chinese Academy of Sciences (2017413) Shenzhen Municipal Funding, China (GJHZ20160229200136090) Shenzhen Discipline Construction Project for Neurobiology, China (DRCSM 1379) Ten Thousand Talent Program, Guangdong Special Support Program, China and Science and Technology Planning Project of Guangdong Province, China (2018B030331001) 

主　　题：Ventral Tegmental Area Aversion Reward Conditioning Behavior 

摘      要：The Ventral Tegmental Area(VTA) is a midbrain structure known to integrate aversive and rewarding stimuli, but little is known about the role of VTA glutamatergic(VGluT2) neurons in these *** activation of VGluT2 soma evokes rewarding behaviors, while activation of their downstream projections evokes aversive behaviors. To facilitate our understanding of these conflicting properties, we recorded calcium signals from VTAVGluT2+ neurons using fiber photometry in VGluT2-cre mice to investigate how this population was recruited by aversive and rewarding stimulation, both during unconditioned and conditioned protocols. Our results revealed that, as a population, VTAVGluT2+neurons responded similarly to unconditioned-aversive and unconditioned-rewarding stimulation. During aversive and rewarding conditioning, the CS-evoked responses gradually increased across trials whilst the US-evoked response remained stable. Retrieval 24 h after conditioning, during which mice received only CS presentation, resulted in VTAVGluT2+ neurons strongly responding to CS presentation and to the expected-US but only for aversive conditioning. To help understand these differences based on VTAVGluT2+ neuronal networks, the inputs and outputs of VTAVGluT2+ neurons were investigated using Cholera Toxin B(CTB) and rabies virus. Based on our results, we propose that the divergent VTAVGluT2+ neuronal responses to aversion and reward conditioning may be partly due to the existence of VTAVGluT2+ subpopulations that are characterized by their connectivity.