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Circulating factors present in the sera of naturally skinny people may influence cell commitment and adipocyte differentiation of mesenchymal stromal cells

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World Journal of Stem Cells 2019年第3期11卷 180-195页

作者： Nicola Alessio Tiziana Squillaro Vincenzo Monda gianfranco Peluso Marcellino Monda Mariarosa AB Melone Umberto Galderisi Giovanni Di Bernardo Department of Experimental Medicine Biotechnology and Molecular Biology Section University of Campania Luigi Vanvitelli Department of Medical Surgical NeurologicalMetabolic and Aging Sciences 2~(nd) Division of Neurology Center for Rare Diseases and InterUniversity Center for Research in Neurosciences University of Campania Luigi Vanvitelli Department of Experimental Medicine Human Physiology and Unit of Dietetic and Sports Medicine Section University of Campania Luigi Vanvitelli Institute Bioscience and BioResources CNR

BACKGROUND Research on physiopathology of obesity may receive new hints from studies on skinny people(SP). These are individuals who show a poor or null gaining of body weight, in spite of high-calorie intake, by far exceeding the body *** To evaluate how circulating factors present in the SP sera may affect adipogenesis of mesenchymal stromal cells(MSCs).METHODS We isolated MSCs from bone marrow of healthy donors with both normal body mass index(BMI) and caloric consumption. MSC cultures were primed with sera collected from SP or normal people(NP). Then biomolecular assays were performed to evaluate effect on proliferation, apoptosis, senescence, cell commitment, and *** SP priming affected adipocyte cell commitment and reduced spontaneous adipogenesis. Moreover, an in-depth analysis of exogenous-induced adipocyte differentiation showed striking differences between differentiation in SP-primed samples compared with NP ones. In adipocytes from SP cultures we observed a reduced size of lipid droplets, an increased expression of adipose triglyceride lipase, along with high mitochondria content and ability to produce ATP in starvation condition. These data and the expression of UCP1 protein, indicated that SP pretreatment produced a bias toward brown adipocyte *** Our data suggest that sera from SP may promote brown adipogenesis rather that white adipocyte differentiation. This finding could explain why SP present normal body composition in spite of an excess of caloric intake. We hypothesize that some circulating components present in the blood of these individuals may favor brown adipogenesis at expense of white adipocyte production.

关键词： Mesenchymal stromal cells Senescence Cytokines Adipogenesis Brown fat

A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

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Journal of Biomedical Science and Engineering 2017年第2期10卷 60-76页

作者： Riccardo Ciuti gianfranco Liguri Post-Graduate School of Clinical Biochemistry University of Florence Florence Italy

A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated. A surfactant present in the reagent causes the lysis of cells in the sample. Glutathione, ascorbic acid, protein thiols and other main reducing substances in the sample react with a specific redox chromogen (2,6-dichlorophenolindophenol) and produce a change in the color of the reagent, which is determined photometrically at 630 nm. This new method quantifies the overall capacity of the sample to reduce a redox chromogen and it requires the minimum volumes and time. The assay can be used on a wide variety of biological samples including whole blood, serum, plasma, tissue extracts and homogenates, food, wine, fruit juice and other beverages. Results are expressed in terms of mmol/L glutathione equivalents. TAC can be assayed manually, on automatic analyzers or micro-plate readers.

关键词： Total Antioxidant Capacity Free Radicals Total Antioxidant Status Oxidative Stress Intracellular TAC

催化膜和催化膜反应器:整合的高效和环保催化过程(英文)

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催化学报 2008年第11期29卷 1152-1158页

作者： Enrico DRIOLI Enrica FONTANANOVA Marcella BONCHIO Mauro CARRARO Martino GARDAN gianfranco SCORRANO Institute on Membrane Technology of the National Research Council (ITM-CNR) University of Calabria Department of Chemical Engineering and Materials University of Calabria ITM-CNR University of Padova

The design of new heterogeneous photooxygenation systems able to employ visible light,oxygen,mild temperatures,and solvent with a low environmental impact has been investigated. In particular,the heterogenization of decatungstate (W10O4-32),a polyoxometalate with photocatalytic activity in oxidation reactions,has been carried out in polymeric membranes of polyvinylidenefluoride. The polymeric catalytic membranes prepared by phase inversion technique have been successfully applied in the aerobic mineralization of phenol in water,which was used as an example of organic pollutant. In order to evaluate the effect of the polymeric environment on the overall catalyst behavior,we have also heterogenized the decatungstate (opportunely functionalized) in perfluorinated membrane made of Hyflon. The photocatalytic composite membranes are characterized by different and tuneable properties depending on the nature of the polymeric micro-environment,in which the catalyst is confined. Moreover,the selective separation function of the membrane results in enhanced performance in comparison with homogeneous reactions.

关键词：催化膜膜反应器催化进程化学分析

Use of the GORE^(®) DrySeal Flex Introducer Sheath to Facilitate Implantation of the Transcatheter Venus P-valve

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Congenital Heart Disease 2021年第3期16卷 197-203页

作者： Matthew I.Jones Matthew Murphy Eric Rosenthal Kevin P.Walsh Damien Kenny Shakeel A.Qureshi gianfranco Butera Department of Congenital Cardiology Evelina London Children’s HospitalGuy’s&St Thomas’NHS Foundation TrustLondonUK Department of Cardiology Mater Misericordiae University HospitalDublinIreland Department of Pediatric Cardiology Our Lady’s Children’s HospitalDublinIreland School of Biomedical Engineering and Imaging Sciences Kings CollegeLondonUK

Objectives:We report our experience of using the 65 cm large diameter GORE^(®) DrySeal Flex Introducer sheath to facilitate transcatheter implantation of the Venus P-valve in the pulmonary ***:Transcatheter implantation of pulmonary valves can be difficult due to rigidity of the valve delivery system or the anatomy of the RVOT and pulmonary artery bifurcation and the risk of iatrogenic damage to the tricuspid valve support *** long sheaths to pass and protect the tricuspid valve may facilitate the ***:Multi-centre registry of patients who underwent transcatheter pulmonary valve implantation of the Venus P-valve using the GORE^(®) DrySeal Flex introducer sheath to facilitate passage of the valve to the right ventricular outflow *** success,time to valve implantation and radiation safety parameters were *** data were compared to a control group of subjects treated between July 2014 and May 2016 with the same valve but without the use of GORE^(®) ***:Between December 2016 and September 2018,the Venus P-valve was successfully deployed through the GORE^(®) DrySeal in 12 *** were no procedure-related *** a control group,10 subjects treated between July 2014 and May 2016 were *** procedure time was significantly shorter in the GORE^(®) DrySeal group compared to the control group 96±27 min vs.164±12 min(p<0.001).Total screening time was significantly shorter in the GORE^(®) DrySeal group(24±11 min)when compared with the control group(32±14 min,p<0.001).Conclusions:We describe a modification to the previously described techniques of implanting the Venus P-valve in the pulmonary position after surgical repair of congenital heart *** our experience,the GORE^(®) DrySeal sheath has considerably facilitated the procedure and reduced the potential risks.

关键词： Congenital heart disease percutaneous pulmonary valve

A case of oesophageal ulcer developed after taking homeopathic pill in a young woman

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World Journal of Gastroenterology 2007年第14期13卷 2132-2134页

作者： Vito D Corleto Lidia D'Alonzo Ermira Zykaj Antonella Carnuccio Francesca Chiesara Cristiano Pagnini Salvatore Di Somma gianfranco Delle Fave Department of Digestive and Liver Diseases Ⅱ School of Medicine University "La Sapienza"Centro Ricerche S. PietroOspedale S. PietroRomeItaly Department of Emergency Medicine Ⅱ School of Medicine University "La Sapienza"RomeItaly Department of Digestive and Liver Diseases Ⅱ School of Medicine University "La Sapienza"RomeItaly

Oesophageal ulcers occur mainly as a result of gastrooesophageal reflux disease （GERD）. However, pillinduced oesophageal ulcers are a fairly common event. The lesion is mainly due to entrapment of the pill and/ or its chemical composition thereof. This case report describes an oesophageal mucosa ulcer occurred in a healthy 35-year old woman who had no previous history of oesophageal disorders and received homeopathic medication. The present case reveals that pill entrapment can occur even in the oesophagus of healthy young individuals and that oesophageal mucosal ulcer can be triggered by substances generally thought devoid of any potentially mucosal aggressive effect.

关键词： Oesophageal ulcer Pill-induced oesophageallesions

Is colonoscopy sufficient for colorectal cancer surveillance in all HNPCC patients?

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World Journal of Gastroenterology 2005年第47期11卷 7541-7544页

作者： Vito D Corleto Ermira Zykaj Paolo Mercantini Emanuela Pilozzi Michele Rossi Antonella Carnuccio Emilio Di Giulio Vincenzo Ziparo gianfranco Delle Fave Department of Digestive and Liver Diseases Ⅱ School of MedicineUniversity "La Sapienza"RomeItaly Department of Digestive and Liver Diseases Ⅱ School of MedicineUniversity "La Sapienza"Centro Ricerche S.Pietro FBFRomeItaly Department of Surgery Ⅱ School of MedicineUniversity "La Sapienza"RomeItaly Department of Pathology Ⅱ School of MedicineUniversity "La Sapienza"RomeItaly Department of Radiology Ⅱ School of MedicineUniversity "La Sapienza"RomeItaly

A 34-year-old male with hereditary non-polyposis colon cancer with a mutation in hMSH2 line is reported. Despite regular colonoscopic follow-up, he developed cecal cancer involving the extraluminal area. Due to sub-occlusive symptoms, the patient was submitted to further colonoscopy, however with no clear evidence of neoplasia. Thin slice multiplanar reconstruction computed tomography CT scan performed thereafter revealed a transmural mass 2.5 cm in size localized near the cecal valve. Discussion is made on the reliability of colonoscopic examinations as well as the need for further investigations in the follow-up of patients at very high risk of right-sided colon cancer, such as male hMSH2 carrier affected by hereditary non-polyposis colon cancer.

关键词： Hereditary non-polyposis colon cancer Spiral CT scan Colonoscopy hMSH2 carrier

Controversies in the treatment of digestive neuroendocrine tumors

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Journal of Cancer Metastasis and Treatment 2016年第1期2卷 304-309页

作者： Maria Rinzivillo Francesco Panzuto gianfranco Delle Fave Department of Digestive and Liver Disease Digestive Neuroendocrine UnitSant’Andrea HospitalSapienza University of Rome00189 RomeItaly

Gastroenteropancreatic neuroendocrine tumors(NETs)have an incidence of 2.39 per 100,000 inhabitants per year,and a prevalence of 35 cases per 100,000 inhabitants;the gap between these rates is due to the relatively long survival time of these tumors,which can be thus considered as chronic oncological ***,more therapeutic options have become available,but criteria for defining timing,priority and sequence of different therapeutic options are still *** review offers an overview of pancreatic and small bowel NETs,critically underlining the issues that still need to be clarified and some controversial issues on the therapeutic approach for NET patients.

关键词： Neuroendocrine tumors therapeutic strategy surgical treatment medical therapies

Dimethyltyrosine, the Viagra of Opioids

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Chemical Research in Chinese Universities 2006年第2期22卷 258-262页

作者： Lawrence H. Lazarus Sharon D. Bryant Severo Salvadori Remo Geurrini gianfranco Balboni Yuko Tsuda Yoshio Okada Peptide Neurochemistry LCBRA National Institute of Environmental Health Sciences Research Triangle Park NC 27709 USA Department of Pharmaceutical Science University of Ferrara I-44100 Ferrara Italy Department of Pharmaceutical Science University of Ferrara I-44100 Ferrara Italy Department of Toxicology University of Cagliary Cagliary 109126 Italy Faculty of Pharmaceutical Science Kobe Gakuin University Nishi-ku Kobe 651-2180 Japan

Introduction The introduction of 2＇ ,6＇-dimethyl-L＇tyrosine （Dmt） at the N-terminus of Tyr-Tic （1,2,3,4-tetra-hydroisoquinoline-3-carboxylic acid ）-containing δ-opioid antagonists enhances receptor affinity and in vitro bioactivity to several orders of magnitude and its application in the formation of ligands with new properties, such as potent inverse agonism. For example, the δ2 agonist,

关键词： Dimethyltryrosine Opioid Agonist

Neurovascular and neuroinflammatory mechanisms associated with mood disorders

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Neuroimmunology and Neuroinflammation 2015年第1期2卷 193-194页

作者： gianfranco Spalletta Gabriele Sani Neuropsychiatry Laboratory Department of Clinical and Behavioral NeurologyScientific Institute for ResearchHospitalisation and Health CareSanta Lucia Foundation00179 RomeItaly Department of Psychiatry and Behavioral Sciences Baylor College of MedicineHoustonTX 77030USA Department of Neurosciences Mental Health and Sensory OrgansSapienza University of RomeSchool of Medicine and PsychologySant’Andrea Hospital00192 RomeItaly Centro Lucio Bini 00193 RomeItaly

According to the World Health Organization,mood disorders are a major source of morbidity,disability and mortality worldwide.[1]In fact,they are the leading cause of suicide.[2]Consistent data have been collected regarding their epidemiology and clinical,neurobiological and neuropsychological characteristics but their etiology and pathophysiology still remain to be *** dearth of data limits the possibility of developing new therapeutic strategies aimed at improving patient outcomes.

关键词： epidemiology disorders mortality