A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples
A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples作者机构：Post-Graduate School of Clinical Biochemistry University of Florence Florence Italy
出 版 物：《Journal of Biomedical Science and Engineering》 (生物医学工程（英文）)
年 卷 期：2017年第10卷第2期
页 面：60-76页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Total Antioxidant Capacity Free Radicals Total Antioxidant Status Oxidative Stress Intracellular TAC
摘 要：A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated. A surfactant present in the reagent causes the lysis of cells in the sample. Glutathione, ascorbic acid, protein thiols and other main reducing substances in the sample react with a specific redox chromogen (2,6-dichlorophenolindophenol) and produce a change in the color of the reagent, which is determined photometrically at 630 nm. This new method quantifies the overall capacity of the sample to reduce a redox chromogen and it requires the minimum volumes and time. The assay can be used on a wide variety of biological samples including whole blood, serum, plasma, tissue extracts and homogenates, food, wine, fruit juice and other beverages. Results are expressed in terms of mmol/L glutathione equivalents. TAC can be assayed manually, on automatic analyzers or micro-plate readers.
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