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中国植物病理学会2023年学术年会

作者： peng qiding CHENG Yongchao YUAN Bowen YANG Yiting XI Dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life Sciences Sichuan University

Endogenous messenger RNAs( mRNAs) are exposed to a complex RNA regulatory network, which is a dynamic regulation in plant cells to maintain a delicate balance between active and inactive mRNAs in the development and fluctuating ***-binding proteins(RBPs) are the major regulators in the RNA regulatory network that control RNA processing in the nucleus and regulate gene expression in the cytoplasm at the post-transcriptional ***-GTPase activating protein SH3 domain binding protein( G3 BP) belongs to a family of RBPs and plays role in viral immunity in ***, the role of G3 BP in response to plant virus infection is largely ***, we showed that the expression of NtG3 BP-L could be first up-regulated and then down-regulated in chilli veinal mottle virus( ChiVMV)-infected Nicotiana tabacum *** overexpression of NbG3 BP-L( homologue of NtG3 BP-L) in Nicotiana benthamiana reduced the accumulation of *** result of yeast two-hybrid assay showed that NtG3 BP-L could specifically interact with6 K2 among the 11 proteins encoded by ***, the interaction between NtG3 BP-L and ChiVMV K2 was further confirmed by bimolecular fluorescence complementation(BiFC) and Co-Immunoprecipitation(Co-IP) *** assay showed that NtG3 BP-L and ChiVMV-6 K2 co-localized in cytoplasm and didn’t fuse at the *** localization also showed that more obvious fusion zone of NtG3 BP-L and ChiVMV-6 K2 appeared in cytoplasm under ChiVMV *** addition,*** plants with transgenic overexpression of NtG3 BP-L conferred resistance to ChiVMV infection,whereas the knockout of NtG3 BP-L in *** promoted ChiVMV ***, our results indicate that NtG3 BP-L plays a positive role in tobacco response to ChiVMV infection by interacting with ChiVMV-6 K2 and thereby affecting the infection of ChiVMV, which provide new insights into understanding the antiviral strategies of plants.

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The miR172/TOE3 Module Mediates the Susceptibility of Tobacco to Virus Infection by Regulating the Expression of PR1

The miR172/TOE3 Module Mediates the Susceptibility of Tobacc...

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中国植物病理学会2023年学术年会

作者： JIAO Bolei LIU Sucen peng qiding ZHOU Jingya YUAN Bowen XI Dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life Sciences Sichuan University

Transcription factors of the APETALA2( AP2) subfamily have been reported to be involved in plant growth, development and abiotic ***, little is known about the function of AP2 in plants response to virus *** this study, TARGET OF EAT3 of Nicotiana tabacum( NtTOE3), one of the clades of AP2 subfamily, was identified as a virus response gene in *** demonstrated that NtTOE3 played a positive role in attenuating virus infection in tobacco by NtTOE3 transgenic lines, including overexpression, RNA interference and knockout mutants of ***, we found that overexpression of NtTOE3 increased the expression of genes related to salicylic acid pathway in transgenic tobacco ***, we verified that NtTOE3 could activate the expression of NtPRl by binding to the promoter of *** of AP2 subfamily are targeted by miR172, so we speculated that miR172 might act as an upstream regulatory factor of *** expected, the result of qRT-PCR analysis showed that the expression of miR172 was correlated with changes of the expression of NtTOE3 in *** plants infected by tobacco mosaic virus( TMV).Importantly,we demonstrated that miR172 was a negative regulator of viral resistance in tobacco.5’ RACE assay was then employed to confirm that NtTOE3 was a target of *** evidence indicated that the miR172/TOE3 module enhanced susceptibility of tobacco to TMV infection by regulating the expression of *** conclusion, our findings reveal a role of miR172/TOE3 module in the regulation of plant immune system and elucidate a novel antiviral molecular mechanism.

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Effects of cucumber mosaic virus infection on physiological characteristic and gene expression of Panax notoginseng

Effects of cucumber mosaic virus infection on physiological ...

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中国植物病理学会2021年学术年会

作者： ZHENG Tianrui peng qiding HUANG Wanying ZHOU Shuhao DONG Jiahong XI Dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan University School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource Yunnan University of Chinese Medicine

Panax notoginseng（Burk.） ***,belonging to the Panax genus under the Araliaceae family,is one of the most highly valued medicinal plants in the *** saponins,the main medicinal active ingredients in *** are synthesized by secondary metabolic pathways and regulated by various *** yield and quality of *** are easily affected by various environmental factors,but the mechanism of the regulation is largely *** the expansion of *** artificial planting area,the occurrence of virus disease in *** is becoming *** this study,the effects of cucumber mosaic virus（CMV） on the growth and saponin synthesis of *** plants were *** results showed that the infection of CMV on *** plants resulted in vein yellow *** chlorophyll content and photosynthesis capacity of virus infected *** plants decreased significantly,whereas a large amount of reactive oxygen species accumulated and the activities of several antioxidant enzymes ***,cell damage and oxidative damage was more serious in virus infected *** *** RNA-Seq and transcriptomic analyses showed that many genes associated with triterpenoid saponin synthesis and disease resistance were up-regulated in *** plants under CMV *** together,our results indicated that virus infection could not only affect the growth of *** plants and induce the expression of defense related genes,but also promote the expression of genes related to the secondary metabolism of *** to improve the disease resistance of *** plants and make use of the up-regulation effect of terpenoid synthesis induced by virus infection is worthy of further study.

关键词： CMV Panax notoginseng Triterpene saponis

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Identification of actinidia chlorotic ringspot-associated virus encoded P4 and P5 as RNA silencing suppressors

Identification of actinidia chlorotic ringspot-associated vi...

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中国植物病理学会2021年学术年会

作者： XU Qianyi peng qiding HAN Hongyan WANG Yunru LI Xiangyangpeng XI Dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan University

Actinidia chlorotic ringspot-associated virus（AcCRaV,genus Emanavirus;family Fimoviridae） is a newly identified negative-stranded RNA virus infecting *** AcCRaV was first reported in China,it has showed a trend of rapid spread and become the main virus infecting kiwifruit in recent *** has a multipartite negative-stranded RNA genome with a single ORF encoded by each genomic *** this study,the functions of P4 and P5 encoded by RNA4 and RNA5 of AcCRaV were *** results of subcellular localization showed that P4 was located in the nucleus and cell membrane,whereas P5 was located only in the nucleus,indicating that P4 and P5 may play different roles in the process of AcCRaV infecting kiwifruit ***,putative proteins encoded by AcCRaV genomic RNA4 and RNA45 were screened for potential RNA silencing suppression activity by using a green fluorescent protein-based reporter agroinfiltration *** results showed that not only P5 had a local RNA silencing suppressor function,but P4,which was predicted as a movement protein,also had a local RNA silencing suppressor function,the results also showed that both P4 and P5 had activities of systemic RNA silencing *** investigation on the possible silencing suppressor active regions in P5 displayed that four partial segments of P5 had RNA silencing suppressor activity similar to that of the full length of ***,pathogenicity test showed that with the help of P4 or P5,potato virus X was more infectious,indicating that both P4 and P5 could enhance the pathogenicity of heterologous *** results provided new date of VSRs from negative-stranded RNA virus,and displayed that multiple VSRs may be essential for protecting AcCRaV from antiviral silencing in the perennial tree host,and may be related to the rapid epidemic of this virus.

关键词： AcCRaV Actinidia RNA silencing

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N gene enhances resistance to Chilli veinal mottle virus and hypersensitivity to salt stress in Nicotiana tobacum

N gene enhances resistance to Chilli veinal mottle virus and...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Yang Ting Lv Rui Xu Zhenpeng Zhu Lisha peng qiding Qiu Long Lin Honghui Xi Dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan University

Plants use multiple mechanisms to fight against pathogen *** resistance(R) gene which specifically mediate plant defense is one of the major mechanisms,while recent studies have shown that R genes have broad spectrum effects in response to various stresses.N gene is the resistance gene specifically resistant to Tobacco mosaic virus(TMV).However,the role of N gene in abiotic stress and other viral response remains *** veinal mottle virus(ChiVMV),a member of Potyvirus genus which contain the largest group of known plant viruses and comprise many agriculturally and economically important *** this study,we investigated the mechanisms by which N regulated plant defense responses under ChiVMV infection and salt ***,we monitored the physiological and molecular changes of tobacco plants under virus *** results showed that when tobacco and tobacco plants were challenged with ChiVMV,tobacco plants displayed more susceptibility at 5 days post infection(dpi),while tobacco plants exhibited more susceptibility at 20 *** accumulation of ROS and NtHIN1 expression were higher in tobacco than tobacco plants at 5 dpi,and the defense gene NtPR1,the activities of antioxidant enzymes and salicylic acid(SA) content were increased in tobaccoplants compared with tobacco *** was suggested that N gene induced hypersensitive response(HR) and enhanced the systemic resistance of plants in response to ChiVMV via a SA-dependent signaling pathway,which could be supported by some studies that systemic necrosis shared HR attributes.N gene induced HR and enhanced the systemic resistance of plants in response to ChiVMV.N gene could also be induced significandy by salt ***,tobacco plants showed hypersensitivity toward increased salt stress,and this hypersensitivity was dependent on abscisic acid and jasmonic acid but not *** together,our results indicated that N gene appeared to important for tobacco plants in response to ChiVMV infect

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四川地区猕猴桃病毒1的RT-PCR检测及外壳蛋白基因序列分析

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园艺学报 2020年第1期47卷 120-126页

作者：彭期定吕蕊杨婷林宏辉席德慧四川大学生命科学学院生物资源与生态环境教育部重点实验室成都610065

为了明确近期新西兰报道的侵染猕猴桃的新病毒——猕猴桃病毒1(Actinidia virus 1,AcV-1)在四川地区猕猴桃上的发生情况及其分子特性,采用RT-PCR对来自四川6个地区疑似感染病毒的90份猕猴桃主栽品种‘红阳’和‘金果’的叶片样品进行检... 详细信息

为了明确近期新西兰报道的侵染猕猴桃的新病毒——猕猴桃病毒1(Actinidia virus 1,AcV-1)在四川地区猕猴桃上的发生情况及其分子特性,采用RT-PCR对来自四川6个地区疑似感染病毒的90份猕猴桃主栽品种‘红阳’和‘金果’的叶片样品进行检测。结果表明,22份样品为AcV-1阳性,检出率为24.4%。将获得的5个AcV-1分离物和已报道的新西兰分离物K75的外壳蛋白(coatprotein,CP)基因序列进行比对,结果表明,分离物间核苷酸序列和氨基酸序列的相似性分别为84.8%~97.1%和89.7%~99.6%,其中除邛崃分离物HYH5与新西兰分离物K75的核苷酸序列相似性较高(97.1%)外,其余4个分离物均低于91%。系统进化分析结果显示,这些分离物主要聚集在3个分支上,分离物HYH5与新西兰分离物K75位于同一分支,其余分离物位于另外2个分支。

关键词：猕猴桃猕猴桃病毒1 RT-PCR CP基因分子特征

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四川省猕猴桃病毒A外壳蛋白基因的序列分析、原核表达及抗血清制备

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果树学报 2019年第9期36卷 1121-1129页

作者：吕蕊彭期定杨婷林宏辉董家红席德慧四川大学生命科学学院·生物资源与生态环境教育部重点实验室成都610065 云南省农业科学院生物技术与种质资源研究所·云南省农业生物技术重点实验室·农业部西南作物基因资源与种质创制重点实验室昆明650205

【目的】研究猕猴桃病毒A(Actinidia virus A,AcVA)的发生及其多样性,为我国猕猴桃产业中AcVA病毒的快速检测、科学防控以及猕猴桃品种选育提供科学依据。【方法】通过巢式PCR从四川省猕猴桃感病叶片中扩增AcVA CP基因序列,进行序列分... 详细信息

【目的】研究猕猴桃病毒A(Actinidia virus A,AcVA)的发生及其多样性,为我国猕猴桃产业中AcVA病毒的快速检测、科学防控以及猕猴桃品种选育提供科学依据。【方法】通过巢式PCR从四川省猕猴桃感病叶片中扩增AcVA CP基因序列,进行序列分析和同源性比对;构建AcVA CP基因的原核表达载体,诱导目的蛋白表达后制备抗血清,用Western blot检测效价。【结果】克隆到3个AcVA四川分离株的完整CP基因序列,成功构建了pET28a-AcVA-DJY4-CP重组质粒,原核表达目的蛋白并制备出特异性抗血清,效价达到1∶5 000。【结论】首次获得了3个AcVA四川分离株,丰富了AcVA的序列多样性,并探索了原核表达的最佳条件,制备了高效价抗血清,为今后AcVA病毒的快速检测和防控提供了技术支撑。

关键词：猕猴桃病毒A 外壳蛋白序列分析原核表达抗血清制备

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河南溹须河流域更新世地貌演变及机制分析

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地域研究与开发 2014年第3期33卷 172-176页

作者：鲁鹏田奇丁邱士可刘彩玲河南省科学院地理研究所郑州450052 河南省地质矿产勘查开发局测绘地理信息院郑州450006

由于同区域密集分布的史前聚落与早期城址密切相关,河南溹须河流域地貌演化研究近年来备受关注。但早期研究侧重于宏观时空尺度下的地貌特征描述,地貌类型划分与演化特征分析不够细致,使得后续研究进展困难。根据野外调查并结合相关文献... 详细信息

由于同区域密集分布的史前聚落与早期城址密切相关,河南溹须河流域地貌演化研究近年来备受关注。但早期研究侧重于宏观时空尺度下的地貌特征描述,地貌类型划分与演化特征分析不够细致,使得后续研究进展困难。根据野外调查并结合相关文献,对溹须河流域更新世地貌特征与演化作了较为细致的分析,对各类地貌的面积与比例进行量算,并对地貌演变的作用机制进行评述。结果显示,新构造运动控制着区域宏观地势的形成,其对区域东部沉陷和西部抬升具有决定作用。第四纪频繁的气候波动强烈影响着区域水流的强度与频率,并以堆积、侵蚀等不同形式对以河流地貌为主的溹须河流域现代地貌的形成起到重要的修塑作用。在构造、气候等作用下,溹须河流域现代地貌逐渐形成。尽管溹须河流域的地貌框架形成于燕山运动时期,但在构造、气候等内营力、外营力的共同作用下,区域当前的地貌特质逐渐构成,而且依然处于持续的演变当中。

关键词：更新世地貌演化驱动机制溹须河流域

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偏最小二乘回归法筛选馥郁香型白酒瓶贮年份特征标记物

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食品与发酵工业 2020年第22期46卷 14-20页

作者：李宜聪樊双喜吉鑫李春扬辛鹏谌松强张泽辉刘凤晖钟其顶中国食品发酵工业研究院有限公司北京100015 全国食品发酵标准化中心北京100015 酒鬼酒股份有限公司湖南吉首416000

为研究馥郁香型白酒瓶贮年份的真实性,通过气相色谱直接进样内标测定馥郁香型白酒样品中48种主要风味组分的含量,结合偏最小二乘回归(partial least squares regression,PLSR)多元统计学方法,建立了馥郁香型成品白酒瓶贮年份PLSR预测模... 详细信息

为研究馥郁香型白酒瓶贮年份的真实性,通过气相色谱直接进样内标测定馥郁香型白酒样品中48种主要风味组分的含量,结合偏最小二乘回归(partial least squares regression,PLSR)多元统计学方法,建立了馥郁香型成品白酒瓶贮年份PLSR预测模型。该模型相关系数(R^2)为0.9534,校正标准偏差(root mean square error of calibration,RMSEC)为0.2159,预测标准偏差(root mean square error of prediction,RMSEP)为0.8384,相对分析误差(relative percent deviation,RPD)为4.6313,模型回归拟合效果好,预测精度高。采用jack-knife方法对PLSR模型回归系数进行显著性检验,筛选出对预测模型有显著影响的特征标记物,发现己酸乙酯、乙醛、辛酸乙酯和正丁醇与馥郁香型白酒瓶贮年份显著相关,为馥郁香型白酒瓶贮年份PLSR预测模型的特征标记物。

关键词：馥郁香型白酒瓶贮年份气相色谱偏最小二乘回归特征标记物

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异戊二烯对苯乙烯-间戊二烯共聚合的影响

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高分子材料科学与工程 2023年第3期39卷 16-25页

作者：熊巧巧古卓玮谢锋历杨帅彭承俊刘文强陈豪陈仕兵张迪青龚林林肖哲袁煜艳佘振银夏金魁许俊东李安刘坤湖南理工学院化学化工学院湖南岳阳414000 中石化巴陵石油化工有限公司湖南岳阳414000

二烯烃-苯乙烯类单体序列结构极大程度上影响相关橡胶类产品(丁苯橡胶、多元集成橡胶)或树脂产品(丁苯树脂)的综合性能。活性阴离子聚合在共聚单体序列类型调控方面具有极大的优势,如严格嵌段序列、渐变嵌段序列、无规序列及交替序列等... 详细信息

二烯烃-苯乙烯类单体序列结构极大程度上影响相关橡胶类产品(丁苯橡胶、多元集成橡胶)或树脂产品(丁苯树脂)的综合性能。活性阴离子聚合在共聚单体序列类型调控方面具有极大的优势,如严格嵌段序列、渐变嵌段序列、无规序列及交替序列等。新型间戊二烯(PD)单体可以通过阴离子共聚合实现其与苯乙烯类单体特殊交替序列共聚物的合成,异戊二烯(IP)阴离子聚合可以实现高顺式1,4-结构聚合物的定向合成。文中以PD、苯乙烯(ST)和IP为共聚单体,在环己烷中(含0.015%四氢呋喃)通过正丁基锂(n-BuLi)引发聚合合成了多元共聚物,重点考察了共聚动力学和共聚物的微观结构。实验结果表明:(1)聚合动力学显示均聚合速率ST>IP>>PD,ST/IP和PD/IP二元共聚显示经典“双S”转化率曲线,ST/PD二元共聚速率与IP均聚速率相当(;2)核磁氢谱和竞聚率数据显示ST/PD二元共聚符合经典交替共聚行为,结合红外数据表明,二元共聚产物1,4-结构含量高于90%,反1,4-结构含量高于85%;(3)三元共聚转化率与瞬时共聚单体组成关系表明,异戊二烯以高顺1,4-结构插入苯乙烯-间戊二烯序列中,三元共聚产物中无明显苯乙烯微嵌段序列和聚间戊二烯序列,无规化程度高(;4)三元共聚均具有明显的活性可控聚合特征,相对分子质量与理论值高度吻合,分子量分布(D)均小于1.10;(5)差示扫描量热分析结果显示,三元序列共聚物是只有唯一Tg的无定形聚合物,共聚单体倾向无规序列排布(;6)基于ST/PD特殊的二元交替共聚,推测了ST/IP/PD可能的三元聚合机理。

关键词：间戊二烯苯乙烯异戊二烯交替共聚无规共聚序列分布

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