The Role of Ras-GTPase Activating Protein SH3Domain Binding Protein in Chilli Veinal Mottle Virus Infected Tobacco
作者单位：Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life Sciences Sichuan University
会议名称：《中国植物病理学会2023年学术年会》
会议日期：2023年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
摘 要：Endogenous messenger RNAs( mRNAs) are exposed to a complex RNA regulatory network, which is a dynamic regulation in plant cells to maintain a delicate balance between active and inactive mRNAs in the development and fluctuating ***-binding proteins(RBPs) are the major regulators in the RNA regulatory network that control RNA processing in the nucleus and regulate gene expression in the cytoplasm at the post-transcriptional ***-GTPase activating protein SH3 domain binding protein( G3 BP) belongs to a family of RBPs and plays role in viral immunity in ***, the role of G3 BP in response to plant virus infection is largely ***, we showed that the expression of NtG3 BP-L could be first up-regulated and then down-regulated in chilli veinal mottle virus( ChiVMV)-infected Nicotiana tabacum *** overexpression of NbG3 BP-L( homologue of NtG3 BP-L) in Nicotiana benthamiana reduced the accumulation of *** result of yeast two-hybrid assay showed that NtG3 BP-L could specifically interact with6 K2 among the 11 proteins encoded by ***, the interaction between NtG3 BP-L and ChiVMV K2 was further confirmed by bimolecular fluorescence complementation(BiFC) and Co-Immunoprecipitation(Co-IP) *** assay showed that NtG3 BP-L and ChiVMV-6 K2 co-localized in cytoplasm and didn’t fuse at the *** localization also showed that more obvious fusion zone of NtG3 BP-L and ChiVMV-6 K2 appeared in cytoplasm under ChiVMV *** addition,*** plants with transgenic overexpression of NtG3 BP-L conferred resistance to ChiVMV infection,whereas the knockout of NtG3 BP-L in *** promoted ChiVMV ***, our results indicate that NtG3 BP-L plays a positive role in tobacco response to ChiVMV infection by interacting with ChiVMV-6 K2 and thereby affecting the infection of ChiVMV, which provide new insights into understanding the antiviral strategies of plants.