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The maternally expressed polycomb group gene OsEMF2a is essential for endosperm cellularization and imprinting in rice

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Plant Communications 2021年第1期2卷 69-84页

作者： Xiaojun Cheng Meiyao pan Zhiguo E Yong Zhou Baixiao Niu Chen Chen Jiangsu Key Laboratory of Crop Genetics and Physiology Key Laboratory of Plant Functional Genomics of the Ministry of EducationJiangsu Key Laboratory of Crop Genomics and Molecular BreedingJiangsu Co-Innovation Center for Modern Production Technology of Grain CropsAgricultural College of Yangzhou UniversityYangzhouChina Key Laboratory of Rice Biology China National Rice Research InstituteHangzhouChina

Cellularization is a key event in endosperm *** group(PcG)genes,such as Fertilization-Independent Seed 2(FIS2),are vital for the syncytium-to-cellularization transition in Arabidopsis *** this study,we found that OsEMF2a,a rice homolog of the Arabidopsis PcG gene Embryonic Flower2(EMF2),plays a role similar to that of FIS2 in regard to seed development,although there is limited sequence similarity between the *** cellularization was observed in osemf2a,associated with an unusual activation of type I MADS-box *** cell cycle was persistently activated in osemf2a caryopses,which was likely caused by cytokinin ***,the overaccumulation of auxin was not found to be associated with the delayed *** OsEMF2a is a maternally expressed gene in the endosperm,a paternally inherited functional allele was unable to recover the maternal defects of *** imprinted rice genes were deregulated in the defective hybrid seeds of osemf2a(♀)/9311(♂)(m9).The paternal expression bias of some paternally expressed genes was disrupted in m9 due to either the activation of maternal alleles or the repression of paternal *** findings suggest that OsEMF2a-PRC2-mediated H3K27me3 is necessary for endosperm cellularization and genomic imprinting in rice.

关键词： endosperm cellularization osemf2a PRC2 genomic imprinting rice

Molecular Phylogeny Supports the Validity of Polypedates impresus Yang 2008

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Asian Herpetological Research 2013年第2期4卷 124-133页

作者： Shulin pan Ningxin DANG Jishan WANG Yitao ZHENG Dingqi RAO Jiatang LI School of Mining and Safety Engineering Yibin University College of Life Sciences Sichuan University Kunming Institute of Zoology Chinese Academy of Sciences Chengdu Institute of Biology Chinese Academy of Sciences

The taxonomy of the Asian tree frogs of the Polypedates leucomystax complex, a group of widespread and morphologically similar species, is very controversial. To ascertain the taxonomic status of these species, we investi- gated the historical relationships among representative samples based on - 2 kb of nucleotide sequences from the mito- chondrial 12S rRNA, tRNAvalinc, and 16S rRNA genes. Our phylogeny resolved five well supported lineages （A, B, C, D and E） in the P leucomystax complex. Polypedates impresus from Yunnan, China, Polypedates cf. mutus and Polypedates cf. megacephalus from Guangxi and Yunnan, China, and Laos constructed Clade C, which is monophyletic. In order to recognize the unique position of this clade, we considered P impresus in Clade C as a valid species. Following our phy- logeny, Chinese Polypedates, corresponding to the other four clades, should include four species： P. mutus （Clade A）; P. braueri （Clade B）; P impresus （Clade C） and P. megacephalus （Clade D）. P Ieucomystax （Clade E） is mainly distributed in the Malaysia, Indonesia and Philippines.

关键词： Chinese Polypedates nucleotide sequence Polypedates leucomystax complex taxonomy

Rapid Sintering of Alumina in Low Pressure RF Plasma

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International Journal of Minerals,Metallurgy and Materials 1999年第2期13卷 133-135页

作者： Wenxia pan Fanxiu Lu Toyonobu Yoshida (InstitUte of Mechanics, Chinese Academy of Sciences, Beijing 100080, China) (Material Science and Engineering School, University of Science and Technology Beliing, Beliing 100083, China) (Department of Metallurgy, Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China Material Science and Engineering School University of Science and Technology Beijing Beijing 100083 China Department of Metallurgy University of Tokyo Tokyo 113 Japan

Three kinds of high-purity A12O3 powders, whose average paticle size is 0. l, 0.3 and 3.0 mp respectively, were used as thestarting power, and their compacts were fired in the radio-frequency plasma generated at a pressure of 80 Pa using N, as the working *** results show that the 0. 1 and 0.3 mp powder compacts can be sintered to nearly the theoretical density within 60 s and thissintering is almost finished in the heating period. It is concluded that the mechanism of liquid sintering, the electric charge effect of Al2O3powder, and the effect of tempetrature gradient in the compacts can affect simultaneously on the rapid densification of the compacts inthe plasma sintering.

关键词： plasma sintering alumina rapid heating

Allosteric conformational changes of G proteins upon its interaction with membrane and GPCR

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Chinese Chemical Letters 2022年第2期33卷 747-750页

作者： Longmei Li Jin Zhang Wenjing Sun Weimin Gong Changlin Tian pan Shi Chaowei Shi Hefei National Laboratory of Physical Science at Microscale and School of Life Sciences University of Science and Technology of ChinaHefei 230027China Department of Chemical Physics at School of Chemistry and Materials Sciences University of Science and Technology of ChinaHefei 230027China

Current resolved structures of GPCRs and G protein complexes provided important insights into G protein activation. However, the binding or dissociation of GPCRs with G protein is instantaneous and highly dynamic in the intracellular environment. The conformational dynamic of G protein still needs to be addressed. In this study, we applied ^(19)F solution NMR spectroscopy to monitor the conformational changes of G protein upon interact with detergent mimicking membrane and receptor. Our results show that there are two states equilibria in the G_(α)in apo states. The interaction of G_(α)with detergents will accelerate this conformational transformation and induce a state that tends to bind to GPCRs. Finally, the G_(α)proteins presented a fully activation state when they coupled to GPCRs.

关键词： 19F solution NMR G protein-coupled receptors Conformational dynamics G protein

Identification of novel mutations in EYA3 and EFTUD2 in a family with craniofacial microsomia:evidence of digenic inheritance

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Frontiers of Medicine 2023年第5期17卷 1006-1009页

作者： Nuo Si Guoqin Zhan Xiaolu Meng Zeya Zhang Xin Huang Bo pan Plastic Surgery Hospital Peking Union Medical College and Chinese Academy of Medical SciencesBeijing100043China

Dear Editor,Craniofacial microsomia(CFM,MIM#164210)is a congenital malformation involving the first and second branchial arch *** phenotype of CFM is highly variable and typically affects the external ear,middle ear,mandible and temporomandibular joint,and facial muscles on the affected *** by craniofacial anomalies,cardiac,vertebral,and central nervous system defects may *** is considered the minimum diagnostic criterion[1,2].

关键词： ani congenital vertebral

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非物质文化遗产系统性保护的措施

中国非物质文化遗产 2025年第1期 14-21页

作者：潘鲁生中国文学艺术界联合会中国民间文艺家协会

非物质文化遗产系统性保护的学理性研究是保护落实的基础。理清家底与资源存量,总结发展经验,有利于推进系统性保护措施落实,形成非物质文化遗产保护与传承的社会氛围,提升公众参与度,使文化遗产项目回归生活。发挥数字技术与产业力量,... 详细信息

非物质文化遗产系统性保护的学理性研究是保护落实的基础。理清家底与资源存量,总结发展经验,有利于推进系统性保护措施落实,形成非物质文化遗产保护与传承的社会氛围,提升公众参与度,使文化遗产项目回归生活。发挥数字技术与产业力量,合理开发,适度应用,以系统性保护适应不同类目发展需要,促进非物质文化遗产资源创造性转化,实现可持续发展。非物质文化遗产系统性保护的宏观规划与微观举措,都要落实于国家、团体、个人权益保护,使传承人成为非物质文化遗产系统性保护的最大受益者,成为传承创新的最大活力来源。完善相关立法,以法律保护文化遗产本身及其传承环境。加强传承群体知识产权保护意识形成有益于文化遗产传承的发展环境,在适应社会需求中实现创新和衍生。

关键词：非物质文化遗产系统性保护传承权益保护创新发展

Effect of nitrate supply on the facilitation between two salt-marsh plants (Suaeda salsa and Scirpus planiculmis)

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Journal of Plant Ecology 2020年第2期13卷 204-212页

作者： Hua Ma Li-Juan Cui Xu pan Wei Li Yu Ning Jian Zhou Institute of Wetland Research Chinese Academy of ForestryBeijing 100091China Beijing Key Laboratory of Wetland Services and Restoration Beijing 100091China Beijing Hanshiqiao National Wetland Ecosystem Research Station Beijing 101309China School of Nature Conservation Beijing Forestry UniversityBeijing 100083China

Aims In estuarine salt-marshes,nitrate supply and soil salinity,which are known as two main environmental drivers,simultaneously affect the interspecific interactions between plant ***,to date,their interactive effects on interspecific interactions have not been closely examined for salt-marsh plant *** Juvenile plants of Suaeda salsa L.(Chenopodiaceae)and Scirpus planiculmis Fr.(Cyperaceae)were grown in rinsed river sand to conduct a greenhouse experiment with three treatment categories:interspecific interaction(mixed culture or monoculture),three salinity levels(1,50 and 100 mmol L^(-1))and three nitrate levels(0.5,5 and 10 mmol L^(-1)).First,height and biomass of all plants were ***,the growth data,relative interaction index and competitive important index of the two species were *** Findings The interspecific interactions between *** and *** were facilitation across the salinity *** promotion of *** growth with high nitrate supply did not enhance the facilitative effect of the species,especially at low ***,high nitrate supply significantly shifted the interspecific interactions of *** from facilitation to competition at high *** results suggest that excessive nitrate application changes the prediction of the stress-gradient hypothesis along a salinity gradient,leading to collapse of the two species coexistence in the *** findings make a contribution to the understanding of how *** and ***,as well as salt-marsh communities,respond to the human modification of estuarine nutritional levels.

关键词： nitrate supply salinity gradient salt-marsh plants facilitation stress-gradient hypothesis

Programmed co-assembly of DNA-peptide hybrid microdroplets by phase separation

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Chinese Chemical Letters 2022年第3期33卷 1545-1549页

作者： Shengtao Yao Yue Liao Rizhao pan Weiping Zhu Yufang Xu Yangyang Yang Xuhong Qian School of Pharmacy Shanghai Key Laboratory of Chemical BiologyEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai 200237China

Biopolymers, including DNA and peptides have been used as excellent self-assembling building blocks for programmable single-component or hybrid materials, due to their controlled molecular ***, combining two assembling principles of DNA-based programmability and peptide-based specific molecular interactions for hybrid structures to microscale has not yet been achieved. In this study,we describe a hybrid microsystem that emerges from the co-assembly of DNA origami structure and short elastin-like polypeptide conjugated oligonucleotides, and initiates liquid-liquid phase separation to generate microdroplets upon heating above the transition temperature. Moreover, the hybrid microdroplets are capable for guest molecule trapping and perform bi-/tri-enzymatic cascades with rate enhancements as open “microreactors”. Our programmed assembled DNA-peptide microsystem represents a new combination of DNA nanotechnology and peptide science and opens potential application routes toward lifeinspired biomaterials.

关键词： Self-assembly Microdroplet DNA origami Phase separation Elastin-like polypeptides

Drug-coated balloons for the treatment of ostial left anterior descending or ostial left circumflex artery lesions:a patientlevel propensity score-matched analysis

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Journal of Geriatric Cardiology 2023年第10期20卷 716-727,I0001-I0005页

作者： Liang pan Wen-Jie LU Zhan-Ying HAN San-Cong pan Xi WANG Ying-Guang SHAN Meng PENG Xiao-Fei QIN Guo-Ju SUN Pei-Sheng ZHANG Jian-Zeng DONG Chun-Guang QIU Department of Cardiovascular Medicine the First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Cardiology Jincheng People’s HospitalJinchengChina Department of Cardiology the Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

BACKGROUND Controversy exists as to the optimal treatment approach for ostial left anterior descending(LAD)or ostial left circumflex artery(LCx)***-coated balloons(DCB)may overcome some of the limitations of drug-eluting stents(DES).Therefore,we investigated the security and feasibility of the DCB policy in patients with ostial LAD or ostial LCx lesions,and compared it with the conventional DES-only *** We retrospectively enrolled patients with de novo ostial lesions in the LAD or LCx who underwent interventional *** were categorized into two groups based on their treatment approach:the DCB group and the DES *** treatment strategies in the DCB group involved the use of either DCB-only or hybrid strategies,whereas the DES group utilized crossover or precise stenting ***-year target lesion revascularization was the primary endpoint,while the rates of major adverse cardiovascular events,cardiac death,target vessel myocardial infarction,and vessel thrombosis were the secondary *** propensity score matching,we assembled a cohort with comparable baseline *** ensure result analysis reliability,we conducted sensitivity analyses,including interaction,and stratified *** Among the 397 eligible patients,6.25%of patients who were planned to undergo DCB underwent DES.A total of 108 patients in each group had comparable propensity scores and were included in the ***-year target lesion revascularization occurred in 5 patients(4.90%)and 16 patients(16.33%)in the DCB group and the DES group,respectively(odds ratio=0.264,95%CI:0.093–0.752,P=0.008).Compared with the DES group,the DCB group demonstrated a lower major adverse cardiovascular events rate(7.84%vs.19.39%,P=0.017).However,differences with regard to cardiac death,non-periprocedural target vessel myocardial infarction,and definite or probable vessel thrombosis between the groups were *** The utilization of

关键词： lesions balloon treatment