检索结果-南通市图书馆

Human leukocyte antigen compatibility and incidence of donorspecific antibodies in pediatric liver transplant recipients

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第33期30卷 3837-3845页

作者： melina U melere Flavia H Feier Jorge neumann Antônio n Kalil Juliana de m montagner Luiza S nader Carolina S da Silva marco Aurélio F Junior Gabriela p coral Guilherme p Bobsin Cristina T Ferreira Department of Hepatology and Liver Transplantation Santa Casa de Porto AlegrePorto Alegre 90050170Rio Grande do SulBrazil Department of Hepato-biliary-pancreatic Surgery and Liver Transplantation Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil postgraduation program in medicine:Hepatology Federal University of Health Sciences of Porto AlegrePorto Alegre 90050-170Rio Grande do SulBrazil Laboratory of Transplantation Immunology Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil

BACKGROUnD Antibody-mediated rejection following liver transplantation(LT)has been increasingly recognized,particularly with respect to the emergence of de novo donor-specific antibodies(DSAs)and their impact on graft *** substantial evidence for adult populations exists,research focusing on pediatric LT outcomes remains *** To investigate the prevalence of human leukocyte antigen(HLA)mismatches and DSA and evaluate their association with rejection episodes after pediatric *** A cohort of pediatric LT recipients underwent HLA testing at Santa Casa de porto Alegre,Brazil,between December 2013 and December *** patients who survived for>30 days after LT with at least one DSA analysis were *** classes I and II and cross-matches were *** presence of de novo DSA(dnDSA)was evaluated at least 3 months after LT using the Luminex®single antigen bead method,with a positive reaction threshold set at 1000 *** episodes were confirmed by liver *** Overall,67 transplanted children were analyzed;61 received grafts from living donors,85%of whom were related to ***-transplant DSA(class I or II)was detected in 28.3%of patients,and dnDSA was detected in 48.4%.The median time to DSA detection after LT was 19.7[interquartile range(IQR):4.3-35.6]*** rejection occurred in 13 patients at follow-up,with C4d positivity observed in 5/13 Liver *** median time to rejection was 7.8(IQR:5.7-12.8)*** presence of dnDSA was significantly associated with rejection(36%vs 3%,pn-free survival rates at 12 and 24 months were 76%vs 100%and 58%vs 95%for patients with dnDSA anti-DQ vs those without,*** Our findings highlight the importance of incorporating DSA assessment into pre-and post-transplantation protocols for pediatric LT *** implications may include immunosuppression minimization strategies based on this analysis in pediatric LT recip

关键词： Human leukocyte antigens Donor-specific antibodies Liver transplantation pediatric Rejection

Telomeric DnA breaks in human induced pluripotent stem cells trigger ATR-mediated arrest and telomerase-independent telomere damage repair

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of molecular Cell Biology 2024年第3期16卷 46-60页

作者： Katrina n.Estep John W.Tobias Rafael J.Fernandez Brinley m.Beveridge F.Brad Johnson Department of pathology and Laboratory medicine University of PennsylvaniaPhiladelphiaPA 19104USA Quantiative Biosciences Merck&Co.Inc.West PointPA 19486USA penn Genomic Analysis core University of PennsylvaniaPhiladelphiaPA 19104USA

Although mechanisms of telomere protection are well-defined in differentiated cells,how stem cells sense and respond to telomere dysfunction,in particular telomeric double-strand breaks(DSBs),is poorly ***,we report the DnA damage signaling,cell cycle,and transcriptome changes in human induced pluripotent stem cells(ipSCs)in response to telomere-internal *** engineer human ipSCs with an inducible TRF1-FokI fusion protein to acutely induce DSBs at *** this model,we demonstrate that TRF1-FokI DSBs activate an ATR-dependent DnA damage response,which leads to p53-independent cell cycle arrest in *** CRISpR–Cas9 to cripple the catalytic domain of telomerase reverse transcriptase,we show that telomerase is largely dispensable for survival and lengthening of TRF1-FokI-cleaved telomeres,which instead are effectively repaired by robust homologous recombination(HR).In contrast to HR-based telomere maintenance in mouse embryonic stem cells,where HR causes ZSCAn4-dependent extension of telomeres beyond their initial lengths,HR-based repair of telomeric breaks is sufficient to maintain ipSC telomeres at a normal length,which is compatible with sustained survival of the cells over several days of TRF1-FokI *** findings suggest a previously unappreciated role for HR in telomere maintenance in telomerase-positive ipSCs and reveal distinct ipSC-specific responses to targeted telomeric DnA damage.

关键词： telomeres telomerase alternative lengthening of telomeres pluripotent stem cells DnA damage double-strand breaks

The effectiveness of different wear indicators in quantifying wear on railway wheels of freight wagons

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Railway Engineering Science 2024年第3期32卷 307-323页

作者： philipe Augusto de paula pacheco m.magelli matheus Valente Lopes pedro Henrique Alves correa n.Zampieri n.Bosso Auteliano Antunes dos Santos Federal Institute of the Southeast of minas Gerais(IF Sudeste mG) Técnico PanamáStreet 45Santos DumontMG 36246-311Brazil Railway Laboratory(Lafer) Universidade Estadual de Campinas(UNICAMP)Mendeleyev Street 200CampinasSP 13083-872Brazil Department of mechanical and Aerospace Engineering Politecnico Di TorinoC.so Duca degli Abruzzi 2410129 TurinItaly

Railway infrastructure relies on the dynamic interaction between wheels and rails;thus,assessing wheel wear is a critical aspect of maintenance and *** paper focuses on the wheel-rail wear indicator T-gamma(Tγ).Amidst its use,it becomes apparent that Tγ,while valuable,fails to provide a comprehensive reflection of the actual material removal and actual contact format,which means that using only Tγas a target for optimization of profiles is not *** this work,three different freight wagons are evaluated:a meter-gauge and a broad-gauge heavy haul vehicles from South American railways,and a standard-gauge freight vehicle operated in Europe,with different axle loads and dissimilar new wheel/rail *** vehicles are subjected to comprehensive multibody simulations on various *** simulations aimed to elucidate the intricate relationship between different wear indicators:Tγ,wear index,material removal,and maximum wear depth,under diverse curves,non-compensated lateral accelerations(A_(nc)),and *** findings showed a correlation of 0.96 between Tγand wear depth and 0.82 between wear index and material removed for the outer *** the results,the Tγis better than the wear index to be used when analyzing wear depth while the wear index is more suited to foresee the material *** results also show the low influence of A_(nc)on wear index and Tγ.By considering these factors together,the study aims to improve the understanding of wheel-rail wear by selecting the best wear analysis approaches based on the effectiveness of each parameter.

关键词： Wear index Wear volume Wear modeling Dynamic simulation

phase composition,conductivity,and sensor properties of cerium-doped indium oxide

在线全文

学校读者我要写书评

暂无评论

nano materials Science 2024年第2期6卷 193-200页

作者： m.I.Ikim G.n.Gerasimov V.F.Gromov O.J.Ilegbusi L.I.Trakhtenberg n.n.Semenov Federal Research Center for Chemical physics of RAS MoscowRussia University of Central Florida OrlandoFLUSA moscow Institute of physics and Technology(State University) DolgoprudnyMoscow RegionRussia Lomonosov moscow State University MoscowRussia

The hydrothermal synthesis of In_(2)O_(3)and CeO_(2)–In_(2)O_(3)is investigated as well as the properties of sensor layers based on these *** the synthesis of In_(2)O_(3),intermediate products In(OH)_(3)and InOOH are formed,which are the precursors of stable cubic(c-In_(2)O_(3))and metastable rhombohedral(rh-In_(2)O_(3))phases,respectively.A transition from c-In_(2)O_(3)to rh-In_(2)O_(3)is observed with the addition of CeO_(2).The introduction of cerium into rh-In_(2)O_(3)results in a decrease in the sensor response to hydrogen,while it increases in composites based on c-In_(2)O_(3).The data on the sensor activity of the composites correlate with XpS results in which CeO_(2)causes a decrease in the concentrations of chemisorbed oxygen and oxygen vacancies in rh-In_(2)O_(3).The reverse situation is observed in composites based on c-In_(2)O_(3).compared to In_(2)O_(3)and CeO_(2)–In_(2)O_(3)obtained by other methods,the synthesized composites demonstrate maximum response to H_(2)at low temperatures by 70–100℃,and have short response time(0.2–0.5 s),short recovery time(6–7 s),and long-term stability.A model is proposed for the dependence of sensitivity on the direction of electron transfer between In_(2)O_(3)and CeO_(2).

关键词： Cerium oxide Indium oxide nanocomposite Hydrothermal method Cubic phase Rhombohedral phase Sensor response conductivity Hydrogen Response/recovery time

Design of a novel Exoskeleton with passive magnetic Spring Self-locking and Spine Lateral Balancing

在线全文

学校读者我要写书评

暂无评论

Journal of Bionic Engineering 2024年第1期21卷 236-255页

作者： Jhon F.Rodríguez-León Betsy D.m.Chaparro-Rico Daniele Cafolla Francesco Lago Eduardo Castillo-Castañeda Giuseppe Carbone Centro de Investigacion en Ciencia Aplicada y Tecnologia Avanzada Unidad Querétaro MecatronicaInstituto Politécnico NacionalCerro Blanco 14176090 Colinas del CimatarioQuerétaroMexico Department of mechanical Energy and Management EngineeringUniversity of CalabriaVia P.Bucci Cubo 46 C87036 RendeItaly Faculty of Science and Engineering Department of Computer ScienceSwansea UniversitySwanseaUK CESTER University of Cluj-NapocaCluj-NapocaRomania

This paper proposes a new upper-limb exoskeleton to reduce worker physical *** proposed design is based on a novel pRRRp(p-prismatic;R-Revolute)kinematic chain with 5 passive Degrees of Freedom(DoF).Utilizing a magnetic spring,the proposed mechanism includes a specially designed locking mechanism that maintains any desired task *** proposed exoskeleton incorporates a balancing mechanism to alleviate discomfort and spinal torsional effects also helping in limb weight *** paper reports specific models and simulations to demonstrate the feasibility and effectiveness of the proposed *** experimental characterization is performed to validate the performance of the mechanism in terms of forces and physical strain during a specific application consisting of ceiling-surface drilling *** obtained results preliminarily validate the engineering feasibility and effectiveness of the proposed exoskeleton in the intended operation task thereby requiring the user to exert significantly less force than when not wearing it.

关键词： Assistive mechanism Exoskeleton Locking mechanism Bionic robot

Exercised blood plasma promotes hippocampal neurogenesis in the Alzheimer's disease rat brain

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2024年第2期13卷 245-255页

作者： Cecilie Skarstad norevik Aleksi m.Huuha Ragnhild n.RФsbjФrgen Linda Hildegard Bergersen Kamilla Jacobsen Rodrigo miguel-dos-Santos Liv Ryan Belma Skender Jose Bianco n.moreira Asgeir Kobro-Flatmoen menno p.Witter nathan Scrimgeour Atefe R.Tari Cardiac Exercise Research Group(CERG) Department of Circulation and Medical ImagingNorwegian University of Science and Technology7491TrondheimNorway Department of neurology and Clinical neurophysiology St.Olavs University Hospital7030TrondheimNorway Institute of Oral Biology University of Oslo0372OsloNorway Department of physiology Federal University of SergipeS~ao Cristov~ao49100-000SergipeBrazil Centre of molecular Inflammation Research Department of Clinical and Molecular MedicineNorwegian University of Science and Technology7491TrondheimNorway Kavli Institute for Systems neuroscience Centre for Neural Computationand Egil and Pauline Braathen and Fred Kavli Centre for Cortical MicrocircuitsNorwegian University of Science and Technology7030TrondheimNorway K.G.Jebsen Centre for Alzheimer’s Disease Norwegian University of Science and Technology7030TrondheimNorway

Background:Exercise training promotes brain plasticity and is associated with protection against cognitive impairment and Alzheimer’s disease(AD).These beneficial effects may be partly mediated by blood-borne *** we used an in vitro model of AD to investigate effects of blood plasma from exercise-trained donors on neuronal viability,and an in vivo rat model of AD to test whether such plasma impacts cognitive function,amyloid pathology,and ***:mouse hippocampal neuronal cells were exposed to AD-like stress using amyloid-βand treated with plasma collected from human male donors 3 h after a single bout of high-intensity *** in vivo studies,blood was collected from exercise-trained young male Wistar rats(high-intensity intervals 5 days/week for 6 weeks).Transgenic AD rats(mcGill-R-Thyl-App)were inj ected 5 times/fortnight for 6 weeks at2 months or 5 months of age with either(a)plasma from the exercise-trained rats,(b)plasma from sedentary rats,or(c)*** function,amyloid plaque pathology,and neurogenesis were *** plasma used for the treatment was analyzed for 23 ***:plasma from exercised donors enhanced cell viability by 44.1%(p=0.032)and reduced atrophy by 50.0%(pn amyloid-β-treated *** vivo exercised plasma treatment did not alter cognitive function or amyloid plaque pathology but did increase hippocampal neurogenesis by~3 fold,regardless of pathological stage,when compared to saline-treated *** of 7 cytokines were significantly reduced in exercised plasma compared to sedentary ***:Our proof-of-concept study demonstrates that plasma from exercise-trained donors can protect neuronal cells in culture and promote adult hippocampal neurogenesis in the AD rat *** effect may be partly due to reduced pro-inflammatory signaling molecules in exercised plasma.

关键词： Cytokines High-intensity interval training Inflammation neurons plasma transfusion

A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

在线全文

学校读者我要写书评

暂无评论

Bio-Design and manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos Santos p.J.Dijkstra C.mota J.Bauer H.Ferreira Rui L.Reis n.neves S.Camarero-Espinosa m.B.Baker L.moroni Department of complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-pT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal pOLYmAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

melt extrusion-based additive manufacturing(mE-Am)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of Am scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(pCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight pCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight pCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(Bmp-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hmSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of Bmp receptor II(BmpRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for mE-Am community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density Click chemistry Human

In-situ construction of grass-like hybrid architecture responsible for extraordinary corrosion performance: Experimental and theoretical approach

在线全文

学校读者我要写书评

暂无评论

nano materials Science 2024年第1期6卷 44-59页

作者： T.Suhartono F.Hazmatulhaq Y.Sheng A.Chaouiki m.p.Kamil Y.G.Ko materials Electrochemistry Group School of Materials Science and EngineeringYeungnam UniversityGyeongsan38541Republic of Korea Research Center for Advanced materials National Research and Innovation Agency(BRIN)South TangerangBanten15314Indonesia

Despite the engineering potential by the co-existence of inorganic and organic substances to protect vulnerable metallic materials from corrosive environments,both their interaction and in-situ formation mechanism to induce the nature-inspired composite remained less *** present work used three distinctive mercaptobenzazole(mB)compounds working as corrosion inhibitors,such as 2-mercaptobenzoxazole(mBO),2-mercaptobenzothiazole(mBT),and 2-mercaptobenzimidazole(mBI)in a bid to understand how the geometrical structure arising from O,S,and n atoms affected the interaction toward inorganic *** compounds that were used here to control the corrosion kinetics would be interacted readily with the pre-existing mgO layer fabricated by plasma *** phenomenon triggered the nucleation of the root network since mB compounds were seen to be adsorbed actively on the defective surface through the active sites in mB ***,the molecule with twin donor atoms adjacent to the mercapto-sites affected the facile growth of the grass-like structures with‘uniform’distribution via molecular self-assembly,which showed better corrosion performance than those with having dissimilar donor atoms with the inhibition efficiency(η)of 97%*** formation mechanism underlying nucleation and growth behavior of mB molecule was discussed concerning the theoretical calculation of density functional theory.

关键词： nature-inspired composite Organic-inorganic interaction corrosion inhibitor Density functional theory Electrochemistry

Increasing the maximum Capacity path in a network and Its Application for Improving the connection Between Two Routers

在线全文

学校读者我要写书评

暂无评论

Tsinghua Science and Technology 2024年第3期29卷 753-765页

作者： Adrian m.Deaconu Javad Tayyebi Department of mathematics and computer Science Transilvania University of BrasovBrasov 500036Romania Department of Industrial Engineering Birjand University of TechnologyBirjand 000097Iran

Abstract:This paper addresses the problem of improving the optimal value of the maximum Capacity path(mCp)through expansion in a flexible network,and minimizing the involved *** only condition applied to the cost functions is to be non-decreasing *** is a non-restrictive condition,reflecting the reality in practice,and is considered for the first time in the ***,the total cost of expansion is a combination of max-type cost(e.g.,for supervision)and sum-type cost(*** building infrastructures,price of materials,price of labor,etc.).For this purpose,two types of strategies are combined:(l)increasing the capacity of the existing arcs,and(l)adding potential new *** different problems are introduced and *** the problems have immediate applications in Internet routing *** first one is to extend the network,so that the capacity of an mcp in the modified network becomes equal to a prescribed value,therefore the cost of modifications is minimized.A strongly polynomial-time algorithm is deduced to solve this *** second problem is a network expansion under a budget constraint,so that the capacity of an mcp is maximized.A weakly polynomial-time algorithm is presented to deal with *** the special case when all the costs are linear,a meggido's parametric search technique is used to develop an algorithm for solving the problem in strongly polynomial *** new approach has a time complexity of O(n^(4)),which is better than the time complexity of O(n4 log(n)of the previously known method from literature.

关键词： maximum Capacity path(mCp) network expansion Internet routing polynomial-time algorithms