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Rice science 2013年第6期20卷 415-426页

作者： p.manimaran G.RAVI KUMAR M.RAGHURAMI REDDY S.JAIN T.BHASKAR RAO S.K.MANGRAUTHIA R.M.SUNDARAM S.RAVICHANDRAN S.M.BALACHANDRAN Biotechnology Laboratory Crop Improvement SectionDirectorate of Rice ResearchRajendranagarHyderabad 500030India

A rapid and reproducible method to develop transgenic plants with enhanced transformation efficiency using Agrobacterium has been developed for the elite indica rice variety BpT 5204. Different rice calli aged from 3 to 30 d were co-cultivated with pre-incubated Agrobacterium suspension culture （LBA4404： pSB1, pCAMBIA1301） and incubated in dark for 3 d. Based on the transient GUS gene expression analysis, 6-day-old young calli showed high transformation frequency followed by 21-day-old ones. Thus, both 6-and 21-day-old calli were used for assessing the stable transformation efficiency. It was observed that the 6-day-old young transformed calli showed about 2-fold higher regeneration frequency when compared with 21-day-old calli. The transformation efficiency was enhanced for young calli to 5.9% compared with 0.8% of the 21-day-old calli. Molecular and genetic analysis of transgenic plants （To） revealed the presence of 1-2 copies of T-DNA integration in transformants and it follows Mendalian ratio in T1 transgenic plants. From the present study, it was concluded that the development of transgenic rice plants in less duration with high regeneration and transformation efficiency was achieved in BpT 5204 by using 6-day-old young calli as explants.

关键词： Agrobacterium-mediated transformation meihod rice callus transformation transgenicplant indica rice

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A novel manganese tungstate nanorod catalyst for the oxidation of styrene with tert-butyl hydroperoxide as oxidant

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Tungsten 2024年第4期6卷 845-858页

作者： Shanmugam manimaran Arumugam pandurangan Gopalakrishnan Govindasamy Department of Chemistry Anna University Energy Cluster School of Engineering University of Petroleum and Energy Studies (UPES)

Catalyst,solvent and process parameters are the keys for the selective oxidation of styrene and other olefins to value added *** MnWO4was synthesized by the one-pot hydrothermal method using pluronic F127 as a surfactant,characterized and evaluated for the oxidation of styrene and different olefins,using tert-butyl hydroperoxide（TBHp） as oxidant.X-ray diffractogram confirmed the phase purity of MnWO4which became further evident from the near surface equimolar proposition of Mn and W in+2 and+6 oxidation states,respectively,as inferred from X-ray photoelectron spectra.N2sorption followed type Ⅳa isotherm with H3 hysteresis loop,starting at the relative pressure（p/p_o） of 0.9,typically of mesoporous materials containing macropores as further confirmed by pore-size *** electron microscope images of MnWO4revealed its nanorod *** the absence of catalyst,styrene conversion was 35%with benzaldehyde as the sole product and addition of 75 g of MnWO4increased the styrene conversion to 97.3%,and the styrene oxide selectivity from 0% to 85% demonstrated its activity and *** the maximum conversion and styrene oxide selectivity optimum temperature,styrene:TBHp molar ratio,catalyst amount and solvent were found to be 70 ℃,1:2,75 mg,and acetonitrile,***4catalyst retained its activity and selectivity for five recycles,confirming its recoverability and *** the kinetic studies,pre-exponential factor and apparent activation energy were found to be 0.206 s-1and 31.5 kJ·mol-1,respectively,and a reaction mechanism was ***4catalyst was active for oxidation of alkenes,cycloolefins and alkenyl aromatics with high selectivity for epoxides.

关键词： Epoxidation Manganese tungstate nanorod Styrene oxidation

Risk assessment framework for power control systems with pMU-based intrusion response system

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Journal of Modern power Systems and Clean Energy 2015年第3期3卷 321-331页

作者： Jie YAN manimaran GOVINDARASU Chen-Ching LIU Ming NI Umesh VAIDYA Department of Electrical and Computer Engineering Iowa State UniversityAmesIA 50011USA Market Engineering MISOCarmelIN 46032USA School of Electrical Engineering and Computer Science Washington State UniversityPullmanWA 99165USA NARI Technology Co.Ltd. Nanjing 211106China

Cyber threats are serious concerns for power *** example,hackers may attack power control systems via interconnected enterprise *** paper proposes a risk assessment framework to enhance the resilience of power systems against cyber *** duality element relative fuzzy evaluation method is employed to evaluate identified security vulnerabilities within cyber systems of power systems *** attack graph is used to identify possible intrusion scenarios that exploit multiple *** intrusion response system(IRS)is developed to monitor the impact of intrusion scenarios on power system dynamics in real *** calculates the conditional Lyapunov exponents(CLEs)on line based on the phasor measurement unit *** system stability is predicted through the values of *** actions based on CLEs will be suggested if power system instability is likely to happen.A generic wind farm control system is used for case *** effectiveness of IRS is illustrated with the IEEE 39 bus system model.

关键词： Cyber security Supervisory control and data acquisition(SCADA) Risk assessment Intrusion response system(IRS) Conditional Lyapunov exponents(CLEs) phasor measurement unit(pMU) Voltage instability

A comparative study of sequential *** type Ⅰ tympanoplasty in patients with bilateral chronic otitis media-Mucosal type

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Journal of Otology 2020年第2期15卷 59-61页

作者： preethi Umamaheswaran Sanjeev Mohanty Vinoth manimaran Sathishkumar Jayagandhi Shiva priya Jeyabalakrishnan Department of ENT Head&Neck SurgeryPanimalar Medical College Hospital&Research InstituteChennaiTamil NaduIndia Department of ENT Head&Neck SurgeryMGM HealthcareChennaiTamil NaduIndia Department of ENT Head&Neck SurgerySri Ramachandra Institute of Higher Education&ResearchChennaiTamil NaduIndia Department of ENT Head&Neck SurgeryKarpagam Faculty of Medical Sciences&ResearchCoimbatoreTamil NaduIndia

Background:patients with bilateral chronic otitis media-mucosal type have been conventionally treated with sequential *** simultaneous tympanoplasty is usually not preferred because of the theoretical risk of iatrogenic sensorineural hearing *** the advent of modern surgical instruments and surgical techniques,the risk is expected to be *** study compares the clinical outcomes in typeⅠtympanoplasty performed simultaneously and ***&methods:This randomized prospective study was carried out in a tertiary care hospital between August 2015 and July 2017.A total of 30 patients were divided into two groups of 15 *** study analyzed the graft uptake,pure tone audiogram findings pre-and post-operatively,duration of surgery and number of hospital visit for each patient and the outco mes were co mpared between both the ***:patients undergoing bilateral simultaneous tympanoplasty had significantly lesser mean duration of surgery and number of hospital visits than the patients undergoing sequential *** uptake and postoperative wound infections were similar in both the *** hearing improvement was significantly better in the bilateral simultaneous tympanoplasty ***,further studies are needed to authenticate this *** of the patients had a postoperative deterioration of hearing or sensorineural hearing ***:Bilateral simultaneous tympanoplasty is not only feasible but also better than sequential tympanoplasty,especially in terms of operating time,follow-up and overall financial implications on the patient.

关键词： Tympanoplasty Chronic otitis media Bilateral tympanoplasty Simultaneous tympanoplasty Sequential tympanoplasty

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Identification of stable internal control genes for accurate normalization of real-time quantitative pCR data in testicular tissue from two breeds of cattle

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Asian pacific Journal of Reproduction 2020年第5期9卷 247-255页

作者： pradeep Nag Ankur Sharma Elango Kamaraj Arumugam Kumaresan Tirtha Kumar Datta Ayyasamy manimaran Nilendu paul Sakthivel Jeyakumar Kerekoppa p.Ramesha Theriogenology Laboratory Veterinary Gynaecologyand ObstetricsSouthern Regional Station of ICAR-National Dairy Research InstituteBengaluru–560030 KarnatakaIndia Animal Genomics Laboratory ICAR-National Dairy Research InstituteKarnal–132001 HaryanaIndia Southern Regional Station of ICAR-National Dairy Research Institute Bengaluru–560030 KarnatakaIndia

Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GApDH,ACTB,RpL23,RpS15A,ATpSF1,GLUT5,HMBS,ATp2B4,ppIA,and BRp to normalize the transcriptional data from testes samples of Zebu and crossbred ***:Total RNA was isolated from testicular tissue of Zebu and crossbred bulls(n=6 each)between 2-8 years of *** was synthesized,and the quantitative real-time polymerase chain reaction(pCR)was *** cycle threshold values were used for the analysis of the stability of *** different statistical algorithms:geNorm,Normfinder,BestKeeper,and RefFinder,were used to assess the stability of these ***:ATpSF1,HMBS,ppIA,and RpS15A were the most reliable and stable ICGs for Zebu testes,and ATpSF1,RpL23,and ppIA for crossbred ***:A panel of stable ICGs(ATpSF1,HMBS,ppIA,RpS15A for Zebu and ATpSF1,RpL23,and ppIA for crossbred)for normalization of gene expression data in testes samples can be helpful for researchers to conduct functional genomics studies at the testicular level in cattle bulls.

关键词： Cattle bulls Gene expression Internal control genes Normalization qpCR Testis

Development of Low Carbon Aquaculture System by Using Zeolite

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Journal of Earth Science and Engineering 2014年第5期4卷 295-310页

作者： Ashamol Antony Baskaran manimaran Kerala University of Fisheries and Ocean Studies Cochin 682506 Kerala India Tamil Nadu Fisheries University Nagapattinam 611001 Tamil Nadu India

Ammonia volatilization is a major process of N （nitrogen） loss that affects the environment. The best way of capturing volatilized ammonia-N could be using zeolite as a good ion exchange medium before it gets either volatilized or nitrified. Thus, captured ammonia-N could be used as a source of inorganic nitrogen in ponds to promote algal production without adding additional organic carbon and BOD （biochemical oxygen demand）. The zeolite used for the study was a commercially available zeolite, （CLINZEX） which was a fine powder （CEC （cation exchange capacity） 3.9-4 meq/g）. The experiment was conducted to assess the difference between manure loaded system and zeolite loaded system in terms of water quality, TAN （total ammoniacal nitrogen） release and algal productivity. The difference between the BOD values recorded in both the controls and treatments utilizing chicken manure as source of manure-N remained mostly above 10 ppm. Similarly, the difference between mean COD （chemical oxygen demand） values of control and treatment tanks always remained above 7 ppm. All the experimental tanks loaded with zeolite samples from chicken manure showed range of variation in TAN values （0.018-0.08 mg/1）. The range of values of chlorophyll a （1,029-5,150 mg/m~） recorded in the treatment tanks was higher than the values （54.6-1347 mg/m3） of chlorophyll a in the control tanks. F-test analysis done using highest mean values of BOD, COD, TAN and chlorophyll a showed a highly significant （p 〈 0.01） variation between the treatment and control tanks and at the same time no significant variation was found between time intervals.

关键词： Zeolite low carbon aquaculture chicken manure organic load inorganic nitrogen.

Isolation of <i>Moraxella</i>sp. from Cases of Keratoconjunctivitis in an Organized Sheep Farm of India

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Open Journal of Veterinary Medicine 2017年第10期7卷 138-143页

作者： Kumaragurubaran Karthik Kaliyaperumal manimaran Ramalingam Mahaprabhu Kulasekaran Shoba Central University Laboratory Madahavarm Milk Colony Tamil Nadu Veterinary and Animal Sciences University Chennai India

Keratoconjunctivitis in sheep causes severe ocular irritation thereby reducing the production performance thus causing economic loss to the farmers. Bacterial cause of keratoconjunctivitis in sheep may be due to Listeria monocytogenes, Moraxella ovis, Mycoplasma conjunctivae, etc. An investigation carried out at Tirupathur, Sivagangai district, Tamil Nadu, India revealed keratoconjunctivitis in sheep (population 100 animals). Isolation and identification of eye swabs from ailing animals (n = 5) showed that the bacteria was Moraxella sp. polymerase chain reaction (pCR) of the isolate was positive for Moraxella sp. Antimicrobial sensitivity testing of the isolate showed that it was sensitive to all antibiotics used in the study. This study reports the presence of Moraxella sp. infection in sheep warranting further studies to know the exact status of this organism so as to prevent keratoconjunctivitis in sheep.

关键词： Moraxella Keratoconjunctivitis Sheep pCR India

Synthesis, Spectral, Anti-Liver Cancer and Free Radical Scavenging Activity of New Azabicyclic Thienoyl Hydrazone Derivatives

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Open Journal of Medicinal Chemistry 2015年第3期5卷 33-47页

作者： M. manimaran A. Ganapathi T. Balasankar Department of Chemistry Annamalai University Annamalai Nagar India Chemistry Section FEAT Annamalai University Annamalai Nagar India

To exploit the potential biological activities of azabicyclic based, seven 2r, 4c-diaryl-3-azabicyclo [3.3.1] nonan-9-one-2’-thienoyl hydrazone were synthesized. The structural elucidation and stereochemistry of these compound assigned by FT-IR, 1H, 13C and 2D NMR spectral data. The Structural Activity Relationship (SAR) of the target compounds were examined for their in vitro anti-proliferative, antioxidant and antimicrobial activities. The initial screen was treated against human liver cancer cell lines (HepG2) with IC50 values determined by MTT assay. Fluoro substitution at para position of phenyl ring compound 12 showed more antiproliferative activity against HepG2 at half maximum inhibitory concentration (IC50 = 3.76 μg/mL) than other target hydrazones. The mechanism of the antitumor action of active compound 12 was investigated through Hoechst stain 33342 analyses. It indicated that the compound inhibited HepG2 cancer cells proliferation by triggering apoptotic cell death. The Free radical scavenging activity of all synthesized compounds were evaluated with , and radicals. The compounds 11 (IC50 rang 3.78 - 4.31 μg/mL) and 15 (IC50 rang 4.61 - 5.16 μg/mL) were exhibited higher free radical scavenging activity than standard BHT drug. Besides, all the target compounds were screened for their in vitro antibacterial and antifungal activity against a spectrum of microbial organisms by using twofold dilution method. These studies proved that halogen substituted compounds 12, 13 and 14 were showed excellent inhibitory potency at lowest minimum inhibitory concentration (MIC) range of 6.25 - 25.5 μg/mL. Nevertheless, multiple mechanisms regulating the antioxidant and anticancer effects of the hybrid molecules need to be further investigations.

关键词： 2-Thienoyl Hydrzones Antioxidant Anticancer Cytotoxicity Antimicrobial Activity

Antiobesity and antihyperlipidemic effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats: An approach to evaluate asymmetrical temperature distribution analysis using thermography

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Chinese Herbal Medicines 2019年第3期11卷 326-331页

作者： Sidhra Syed Zameer Ahmed Syed Zameer Ahmed Khader Krishnaveni Radhakrishnan Vanmathi Marimuthu Muniraj Chinnusamy Venkatesan Thangavel Karamchand Ravi manimaran Vetrivel Department of Biotechnology K.S.Rangasamy College of TechnologyTiruchengodeTamil Nadu 637215India Department of Electrical and Electronics Engineering K.S.Rangasamy College of TechnologyTiruchengodeTamil Nadu 637215India

Objective: Obesity and hyperlipidemia is the major cause of many pathological diseases with an increase side effects using allopathic drugs. The present study focuses on the effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats and associated ***: In vitro radical scavenging activity of I. coccinea was assessed using DppH, FRAp and hydrogen peroxide. In vivo antiobesity and antihyperlipidimic activity of I. coccinea was tested in Triton X-100 induced hyperlipidemic rats and assessed for its biochemical parameters in blood and tissue *** relationship between physiological responses and regulation of body temperature was investigated by using animal surface temperature images captured with infrared ***: The results of mineral analysis, antioxidant, total flavonoid and phenolic content represented high amount of mineral and had the potential to scavenge free radicals tested with DppH, FRAp and hydrogen peroxide radicals with dose dependent activity. The highest activity was observed in aqueous extract, DppH with 71.5% inhibition, FRAp with 56.8%, H2 O2 with 33% activity at 100 μg/mL *** X-100 induced hyperlipidemic rats when treated with I. coccinea aqueous extract showed significant activity by regulating the biochemical parameters and maintaining the lipid profile by decreasing TC,LDL-C, VLDL-C, TG and improving HDL-C levels. Similarly, the elevated levels of creatinine, urea, uric acid,AST, ALT, ALp due to induction of hyperlipidemia, were brought back to near normal levels after treatment with I. coccinea. The levels of tissue anti-oxidants enzymes like SOD and CAT were also found to be improved in treated I. coccinea groups. The whole body asymmetrical temperature distribution analysis showed that significant decreases in temperature was observed in obesity induced groups but a gradual increase in temperature(2%–5%) was observed after ***: Thus, the results indicated that

关键词： anti-oxidant hyperlipidemia Ixora coccinea Curtis obesity thermography Triton X-100

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Oesophageal carcinoma mimicking a submucosal lesion: A case report

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World Journal of Gastrointestinal Endoscopy 2019年第11期11卷 541-547页

作者： Revathy Marimuthu Shanmugam Chitra Shanmugam manimaran Murugesan Muthukumaran Kalyansundaram Sathya Gopalsamy Amiya Ranjan Department of Medical Gastroenterology Government Stanley Medical College

BACKGROUND Oesophageal cancer is the fourth most common cause of cancer-related deaths in *** squamous cell carcinomas(ESCCs)arise from the epithelial layer,and commonly present as polypoidal,ulcerative or ulceroproliferative growth in the oesophageal *** contrast,oesophageal submucosal tumours are a distinct group of tumours arising from the mesenchyme(examples include leiomyoma,fibrovasculoma,lipoma,granular cell tumour or carcinoid),and mostly do not breach the *** submucosal tumours are a distinct group of tumours arising from the mesenchyme,and mostly do not breach the *** intramural growth of an advanced primary ESCC is an exceedingly rare presentation,with only six cases reported in the literature thus *** herein report a case of primary ESCC with complete intramural invasion that endoscopically mimics a submucosal *** SUMMARY A 50 year old male presented with a progressive mechanical type of dysphagia for one *** history was significant,including squamous cell carcinoma of the tongue that was treated with surgery and chemoradiation 1 year *** gastrointestinal endoscopy revealed a large,hemispherical lesion with normalappearing overlying mucosa about 4 cm×5 cm in size extending from 30-34 cm from *** patient underwent endoscopic ultrasound(EUS),and a fineneedle biopsy was performed,which was suggestive for squamous cell *** herein report a case of primary ESCC with complete intramural invasion,endoscopically mimicking a submucosal *** diagnosis could be established only by a EUS-guided *** This case report highlights that intramural ESCC may look like a submucosal lesion in endoscopy,and EUS biopsy is needed for final diagnosis.

关键词： Intramural esophageal squamous cell carcinoma Submucosal lesion of esophagus Endoscopic ultrasound Case report