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Analysis of antisym metric cross-ply laminates using high-order shear deformation theories:a meshless approach

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Acta Mechanica Sinica 2020年第5期36卷 1078-1098,I0003页

作者： D.E.S.Rodrigues J.Belinha L.M.J.S.Dinis R.M.natal Jorge Institute of Science and Innovation in Mechanical and Industrial Engineering 4200-465 PortoPortugal Department of Mechanical Engineering School of EngineeringPolytechnic of Porto(ISEP)4200-072 PortoPortugal Department of Mechanical Engineering Faculty of EngineeringUniversity of Porto(FEUP)4200-465 PortoPortugal

For many years finite element method(FEM)was the chosen numerical method for the analysis of composite ***,in the last 20 years,the scientific community has witnessed the birth and development of several meshless methods,which are more flexible and equally accurate numerical *** meshless method used in this work is the natural neighbour radial point interpolation method(NNRPIM).In order to discretize the problem domain,the NNRPIM only requires an unstructured nodal ***,using the Voronoi mathematical concept,it enforces the nodal connectivity and constructs the background integration *** NNRPIM shape functions are constructed using the radial point interpolation *** this work,the displacement field of composite laminated plates is defined by high-order shear deformation *** the end,several antisymmetric cross-ply laminates were analysed and the NNRPIM solutions were compared with the *** obtained results show the efficiency and accuracy of the NNRPIM formulation.

关键词： Antisymmetric cross-ply laminates High-order shear deformation theories Meshless methods Naturalneighbour radial point interpolation method(NNRPIM)

Effect of a program to control perioperative blood glucose on the incidence of nosocomial infections in patients with diabetes: A pilot study

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Journal of Diabetes Mellitus 2012年第2期2卷 238-244页

作者： Javier Ena Rosa casan Maria Jose Carratala Edith Leutscher Department of Internal Medicine Hospital Marina BaixaAlicanteSpain Division of Endocrinology and Metabolism Hospital Marina BaixaAlicanteSpain Division of Preventive Medicine Hospital Marina BaixaAlicanteSpain

Aim: To evaluate the safety and effectiveness of a pilot program to control perioperative blood glucose in patients with diabetes. Methods: A pre-post intervention study was conducted in a 280-bed hospital in Spain. In the year 2008 we implemented perioperative insulin protocols aimed at blood glucose values from 80 to 180 mg/dL. Surgical patients with diabetes admitted on year 2009 (intervention group) were compared with a control group of patients with diabetes admitted for surgery on year 2007, matched 1:1 by traditional wound class. Results: We analyzed 96 patients. Implemented protocols were followed in 48% of patients intra-operatively and 75% of patients postoperatively. Patients in the intervention group had reductions in blood glucose at surgery 150 +/- 61 mg/dL vs. 172 +/- 53 mg/dL;p = 0.05), greater proportion of target glucose values throughout hospitalization (67% vs. 55%;p = 0.07), and reductions in the incidence of nosocomial infections after controlling for confounders (Odds Ratio: 0.20;95% Confidence intervals: 0.06 - 0.72;p = 0.014) when they were compared with the control group: The incidence of hypoglycemia was similar between two groups (0.12% vs. 0.10%, p = 0.867), respectively. Conclusion: Although our protocol needs improvements to increase implementation it was useful to control blood glucose safely and for reducing nosocomial infections.

关键词： Diabetes Insulin/Administration and Dosage/Therapeutic Use Surgical Site Infection Nosocomial Infection

Considerations on pancreatic exocrine function after pancreaticoduodenectomy

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World Journal of Gastrointestinal Oncology 2014年第9期6卷 325-329页

作者： Francisco José Morera-Ocon Luis Sabater-orti Elena Muoz-Forner Jaime Pérez-Griera Joaquín Ortega-Serrano Department of General Surgery Hospital Clinico de Valencia46010 ValenciaSpain Clinical Analysis Laboratory Hospital Clinico de Valencia

The pancreaticoduodenectomy(PD) procedure may lead to pancreatic exocrine and endocrine *** are several types of reconstruction for this kind of ***(PG) was introduced to reduce the rate of postoperative pancreatic *** some randomized control trials have shown no differences regarding pancreatic leakage between PG and pancreaticojejunostomy(PJ),recently some reports reveal benefits from the PG over the *** surgeons concern about the performing of the PG and inactivation of pancreatic enzymes being in contact with the gastric juice,and the detrimental results over the exocrine pancreatic *** pancreatic exocrine function can be measured with direct and indirect *** tests have the highest sensitivity and specificity for detection of exocrine insufficiency but require tube *** the tubeless indirect tests,the van de Kamer stool fat analysis remains the standard to diagnose fat *** patient compliance and time consuming makes it not so suitable for its clinical *** immunoreactive elastase test is employed for screening of exocrine insufficiency,is not cumbersome,and has been used to study pancreatic function after *** analyze the FE1 levels in our patients after the PD with two types of reconstruction,PG and PJ,and we discuss some considerations about the pancreaticointestinal drainage method after pancreaticoduodenectomy.

关键词： Pancreaticoduodenectomy Pancreaticogastrostomy Pancreaticojejunostomy Pancreatic exocrine function Fecal elastase

In vitro adherence of conjunctival bacteria to different oculoplastic materials

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International Journal of Ophthalmology(English edition) 2018年第12期11卷 1895-1901页

作者： Alvaro Toribio Honorina Martinez-Blanco Leandro Rodriguez-Aparicio Miguel A.Ferrero Teresa Marrodán Isabel Fernández-natal Department of Ophthalmology University Hospital of Leon Leon 24071Spain Department of Molecular Biology University of LeonLeon 24071Spain Department of Clinical Microbiology University Hospital of LeonLeon 24071Spain

AIM: To investigate the resistance to bacterial adhesion of materials used in oculoplastic surgery, particularly materials used in the manufacture of orbital ***: Seven organisms of conjunctival flora(two strains of Staphylococcus epidermidis and one strain each of Staphylococcus aureus, Staphylococcus hominis, Corynebacterium amycolatum, Acinetobacter calcoaceticus, and Serratia marcescens) were selected. A lactic acid bacterium(Lactobacillus rhamnosus) was also included as positive control because of its well-known adhesion ability. Eight materials used to make oculoplastic prostheses were selected(glass, steel, polytetrafluoroethylene, polymethylmethacrylate, silicone from orbital implants, commercial silicone, porous polyethylene, and semismooth polyethylene). Materials surfaces and biofilms developed by strains were observed by scanning electron microscopy. Kinetics of growth and adhesion of bacterial strains were determined by spectrophotometry. Each strain was incubated in contact with plates of the different materials. After growth, attached bacteria were re-suspended and colony-forming units(CFUs) were counted. The number of CFUs per square millimetre of material was statistically ***: A mature biofilm was observed in studied strains except Staphylococcus hominis, which simply produced a microcolony. Materials showed a smooth surface on the microbial scale, although steel exhibited 1.0-μm-diameter grooves. Most organisms showed significant differences in adhesion according to the material. There were also significant differences in thetotal number of CFUs per square millimetre from each material(P=0.044). CFU counts were significantly higher in porous polyethylene than in silicone from orbital implants(P=0.038).CONCLUSION: Silicone orbital implants can resist microbial colonization better than porous polyethylene implants.

关键词： conjunctival flora microbial adhesion biofilm orbital implant oculoplastic prosthesis

Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

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Journal of Forestry Research 2022年第5期33卷 1573-1584页

作者： Leandro Silva de Oliveira Gilvano Ebling Brondani Letícia Vaz Molinari Rafaella Zanetti Dias Gustavo Leal Teixeira Antônio natal Gonçalves Marcílio de Almeida Institute of Agricultural Science Federal University of Minas GeraisUniversity Campus1000Universitária Ave.Montes ClarosMG 39404-547Brazil Laboratory of In Vitro Culture of Forest Species Department of Forestry SciencesFederal University of LavrasUniversity CampusCentral AvenueLavrasMG 37200-900Brazil Department of Biological Sciences “Luiz de Queiroz”College of AgricultureUniversity of São Paulo(ESALQ/USP)University Campus11Pádua Dias AvenuePiracicabaSP 13418-900Brazil

Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana *** enables large-scale production of plants from transformed *** study evaluates the effect ofα-naphthaleneacetic acid(NAA),thidiazuron(TDZ)and benzylaminopurine(BAP)on the organogenesis of *** from hypocotyls and cotyledonary *** the induction stage,hypocotyls and cotyledonary leaves were established in a Murashige and Skoog(MS)culture medium supplemented with NAA or *** tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation *** buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89μM BAP and 0.05μM *** leaves exhibited the best in vitro *** induction of adventitious buds occurred only in calluses induced from *** the differentiation stage,4.4μM BAP treatment promoted an increase of adventitious bud ***-cuttings from regenerated shoots were acclimatized and rooted ex vitro in *** results confirm the establishment of an efficient protocol for the in vitro regeneration of *** by indirect organogenesis,providing new insights regarding cloning of this species.

关键词： Micropropagation Recalcitrant species Plant growth regulator Explants source

Conjunctival flora in anophthalmic patients: microbiological spectrum and antibiotic sensitivity

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International Journal of Ophthalmology(English edition) 2019年第5期12卷 765-773页

作者： Alvaro Toribio Teresa Marrodán Isabel Fernández-natal Honorina Martínez-Blanco Leandro Rodríguez-Aparicio Miguel á.Ferrero Department of Ophthalmology University Hospital of León Department of Clinical Microbiology University Hospital of León Department of Molecular Biology University of León

AIM: To identify the spectrum and susceptibility pattern of isolated microorganisms from conjunctival flora of anophthalmic ***: A cross-sectional clinical study including 60 patients with unilateral anophthalmia. Patients with use of antibiotic drops in their socket during the last month were also included. From each patient, three microbiological samples were taken from the lower conjunctival sac(healthy eye, pre-prosthesis, and retro-prosthesis space of socket). The 180 samples obtained were cultured. Isolates were identified and their antibiotic sensitivities were ***: A total of 251 isolates were recovered(62 isolates from healthy eye, 93 from pre-prosthesis, and 96 from retro-prosthesis space). The most common organism was Staphylococcus epidermidis, in both healthy eyes(64.5%) and sockets(45.5%). Altogether, coagulasepositive Staphylococci, Streptococci, and Gram-negative bacteria accounted for less than 15% of isolates in healthy eyes and more than 35% in sockets. Regarding the antibiotic sensitivities, there were no significant differences between isolates from sockets and healthy eyes. Nine patients recognized the use of self-prescribed antibiotic drops in their socket. In the healthy eyes of these subjects, Gram-positive microorganisms showed significantly greater resistance to aminoglycosides and ***: Sockets of anophthalmic patients show a greater number of pathogens compared to healthy *** use of antibiotic drops in the socket promotes a resistant flora not only in the socket but also in the healthy eye. Quinolones and macrolides may be better therapeutic options than aminoglycosides for treating conjunctivitis of anophthalmic sockets, since these antibiotics are less active against Staphylococcus epidermidis.

关键词： socket ocular prosthesis antibiotic resistance microflora conjunctival dysbiosis

The analysis of composite laminated beams using a 2D interpolating meshless technique

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Acta Mechanica Sinica 2018年第1期34卷 99-116页

作者： S.H.M.Sadek J.Belinha M.P.L.Parente R.M.natal Jorge J.M.A.César de Sá A.J.M.Ferreira Department of Mechanical Engineering Faculty of EngineeringUniversity of Porto Institute of Mechanical Engineering and Industrial Management University of Porto

Laminated composite materials are widely implemented in several engineering constructions. For its relative light weight, these materials are suitable for aerospace, military, marine, and automotive structural applications. To obtain safe and economical structures, the modelling analysis accuracy is highly relevant. Since meshless methods in the recent years achieved a remarkable progress in computational mechanics, the present work uses one of the most flexible and stable interpolation meshless technique available in the literature—the Radial Point Interpolation Method(RPIM).Here, a 2 D approach is considered to numerically analyse composite laminated beams. Both the meshless formulation and the equilibrium equations ruling the studied physical phenomenon are presented with detail. Several benchmark beam examples are studied and the results are compared with exact solutions available in the literature and the results obtained from a commercial finite element software. The results show the efficiency and accuracy of the proposed numeric technique.

关键词： Radial point interpolators RPIM Laminated beam Meshless method

In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

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Journal of Forestry Research 2011年第2期22卷 209-215页

作者： Yurika H.Komatsu Katherine Derlene Batagin-Piotto Gilvano Ebling Brondani Antnio natal Gonalves Marcílio de Almeida Graduate Program in Forest Resources Department of Forest Sciences"Luiz de Queiroz" College of AgricultureUniversity of So Paulo(ESALQ/USP)Avenida Pádua Dias11PiracicabaSo PauloBrazil Graduate Program in Biochemistry of Plants Department of Biological Sciences"Luiz de Queiroz" College of AgricultureUniversity of So Paulo(ESALQ/USP)Avenida Pádua Dias11PiracicabaSo Paulo

Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram（4-amino-3,5,6-trichloropicolinic acid） at different concentrations of carbohydrate under the same conditions with presence or absence of *** our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus *** were transferred in MS medium supplemented with sucrose,fructose and glucose（control,2%,4% and 6%）.Results show that Picloram induced the callogenesis in leaf *** secondary embryogenesis was formed in yellow-globular *** sucrose as carbohydrate source in the absence of light was more efficient to induce *** was more efficiency in the presence of *** induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.

关键词： poaceae tissue culture callus induction cell competence determination

Low temperature,IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings

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Journal of Forestry Research 2012年第4期23卷 583-592页

作者： Gilvano Ebling Brondani Francisco JoséBenedini Baccarin Heron Wilhelmus de Wit Ondas JoséLuiz Stape Antonio natal Gonçalves Marcilio de Almeida Department of Forest Engineering Federal University of Mato Grosso(UFMT)Avenida Fernando Corrêa da Costa2367ZC 78060-900CuiabáMato GrossoBrazil. Department of Forest Sciences "Luiz de Queiroz"College of AgricultureUniversity of S o Paulo(ESALQ/USP)Avenida Pádua Dias11POB 9ZC 13418-900PiracicabaSo PauloBrazil. Department of Biological Sciences "Luiz de Queiroz"College of AgricultureUniversity of S o Paulo(ESALQ/USP)Avenida Pádua Dias11POB 9ZC 13418-900PiracicabaSo PauloBrazil. Department of Forest and Environmental Sciences North Carolina State UniversityNC 27695USA.

Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA （indole-3-butyric acid） concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 （immediate planting）, 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions： 0 （free of IBA）, 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days （0 （immediate planting）, 7, 14, 21 and 28 days）, destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.

关键词： rhizogenesis plant cloning mini-cutting technique histological analysis indole-3-butyric acid.