Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

作     者：Leandro Silva de Oliveira Gilvano Ebling Brondani Letícia Vaz Molinari Rafaella Zanetti Dias Gustavo Leal Teixeira Antônio Natal Gonçalves Marcílio de Almeida Leandro Silva de Oliveira;Gilvano Ebling Brondani;Letícia Vaz Molinari;Rafaella Zanetti Dias;Gustavo Leal Teixeira;Ant?nio Natal Gon?alves;Marcílio de Almeida

作者机构：Institute of Agricultural ScienceFederal University of Minas GeraisUniversity Campus1000Universitária Ave.Montes ClarosMG 39404-547Brazil Laboratory of In Vitro Culture of Forest SpeciesDepartment of Forestry SciencesFederal University of LavrasUniversity CampusCentral AvenueLavrasMG 37200-900Brazil Department of Biological Sciences“Luiz de Queiroz”College of AgricultureUniversity of São Paulo(ESALQ/USP)University Campus11Pádua Dias AvenuePiracicabaSP 13418-900Brazil 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2022年第33卷第5期

页      面：1573-1584页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：CAPES (Coordination for the Improvement of Higher Personnel, Brazil) for their scholarship CNPq (National Counsel of Technological and Scientific Development, Brazil) IPEF (Forestry Science and Research Institute, Brazil) for technical support 

主　　题：Micropropagation Recalcitrant species Plant growth regulator Explants source 

摘      要：Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana *** enables large-scale production of plants from transformed *** study evaluates the effect ofα-naphthaleneacetic acid(NAA),thidiazuron(TDZ)and benzylaminopurine(BAP)on the organogenesis of *** from hypocotyls and cotyledonary *** the induction stage,hypocotyls and cotyledonary leaves were established in a Murashige and Skoog(MS)culture medium supplemented with NAA or *** tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation *** buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89μM BAP and 0.05μM *** leaves exhibited the best in vitro *** induction of adventitious buds occurred only in calluses induced from *** the differentiation stage,4.4μM BAP treatment promoted an increase of adventitious bud ***-cuttings from regenerated shoots were acclimatized and rooted ex vitro in *** results confirm the establishment of an efficient protocol for the in vitro regeneration of *** by indirect organogenesis,providing new insights regarding cloning of this species.