Low temperature,IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings
Low temperature,IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings作者机构:Department of Forest EngineeringFederal University of Mato Grosso(UFMT)Avenida Fernando Corrêa da Costa2367ZC 78060-900CuiabáMato GrossoBrazil. Department of Forest Sciences"Luiz de Queiroz"College of AgricultureUniversity of S o Paulo(ESALQ/USP)Avenida Pádua Dias11POB 9ZC 13418-900PiracicabaSo PauloBrazil. Department of Biological Sciences"Luiz de Queiroz"College of AgricultureUniversity of S o Paulo(ESALQ/USP)Avenida Pádua Dias11POB 9ZC 13418-900PiracicabaSo PauloBrazil. Department of Forest and Environmental SciencesNorth Carolina State UniversityNC 27695USA.
出 版 物:《Journal of Forestry Research》 (林业研究(英文版))
年 卷 期:2012年第23卷第4期
页 面:583-592页
核心收录:
学科分类:0830[工学-环境科学与工程(可授工学、理学、农学学位)] 0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 0901[农学-作物学]
基 金:financially by FAPESP (So Paulo Research Foundation,Brazil) CAPES (Coordination for the Improvement of Higher Level Personnel,Brazil)
主 题:rhizogenesis plant cloning mini-cutting technique histological analysis indole-3-butyric acid.
摘 要:Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA (indole-3-butyric acid) concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 (immediate planting), 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions: 0 (free of IBA), 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days (0 (immediate planting), 7, 14, 21 and 28 days), destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.