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Presence of GABAergic neuron progenitors in the mouse neocortex and spinal cord

Presence of GABAergic neuron progenitors in the mouse neocor...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： nobuaki tamamaki Department of Morphological Neural Science Graduate School of MedicineKumamoto UniversityKumamoto 860-8556Japan

＜正＞It has been suggested that the main source of GAB Aergic neurons in the rodent neocortex and spinal cord is in the subpallium and the ventricular zone of the spinal cord,*** study revealed a production of GABAergic neurons in the mouse neocortex and the spinal mantle *** immunohistochemistry,we detected that about 25%of the GFP-positive cells in the cortical subventricular zone（SVZ） of GAD67-GFP knock-in mouse at E18 were positive for Ki- 67 and some of them were undergoing mitosis or in the DNA synthesis *** cells obtained from the deeper half of the neocortex in GAD67-GFP knock-in mouse at E18 were further used for immunocytochemistry and single-cell RT-PCR. Immunostaining results showed that all GFP-positive cells were positive for *** were also positive for MAP2（99.1%） and Tuj1（95.2%）.We then found some GFP-positive cells were characterized by cell cycle makers Ki-67（14. 4%）,P-H3（4.7%） and BrdU（0.8%）.Single-cell PCR showed that 18.5%of GFP-positive cells expressed Ki-67 and all GFP/Ki- 67 cells also expressed GAD67,MAP2 and *** also detected that about 1.6%,0.3%and 0.5%of GFP-positive cells in the spinal mantle layer at E17 were positive for Ki-67,P-H3 and BrdU,*** also revealed that some GFP-positive cells dissociated from the E17spinal mantle layer were positive for Ki-67（2.1%）,P-H3（0.5%） and BrdU（0.8%）.The present results indicate that neuronal and cell-cycle markers are colocalized in GABAergic-neuron progenitors that contribute to the production of neocortical and spinal GABAergic interneurons.

关键词： GABA progenitor development cortex spinal cord

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Presence and characterization of intermediate GAB Aergic-neuron progenitors in the neocortex

Presence and characterization of intermediate GAB Aergic-neu...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： nobuaki tamamaki Department of Morphological Neural Science School of MedicineKumamoto UniversityKumamoto 860-8556Japan

＜正＞Objective In the embryonic neocortex,neural cells are generated in the inner-most layer of the pallium,the ventricular zone（VZ）,and express cell proliferation markers,while differentiated neurons accumulate in the outer layers and express neuronal ***,the nature of intermediate neuron progenitors is not ***,we show that the SVZ of the perinatal mouse neocortex contains intermediate GABAergic-neuron progenitors（IGNPs）,whose fate seems to be the neuronal *** GFP-positive cells in the SVZ of GAD67-GFP knock-in mice by immunostaining, single-cell RT-PCR,and single-cell microarray *** We found that a substantial number expressed both neuronal and cell-cycle *** neuronal markers included GAD67,MAP2,βⅢ-tubulin,and those for proliferating cells were Ki-67,PCNA,cyclin-D *** addition,genes involved in asymmetric cell division,such as Notch3,deltal, delta4,and Numb had present calls in the microarray *** the data indicated that the cell-cycle phases are not for apoptosis,but for cell ***,the GFP-positive cells incorporated BrdU and produced pairs of BrdU andβⅢ- tubulin double-positive cells in *** Taken together,these data indicate that IGNPs divide asymmetrically or symmetrically,and produce only cells of the neuronal lineage in the developing neocortex.

关键词： GABAergic neuron progenitor development neocortex mouse

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Leptomeninges as a new pool for neurogenesis in adult mammalian cerebral cortex following amygdala kindling

Leptomeninges as a new pool for neurogenesis in adult mammal...

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中国解剖学会2015年年会

作者： Chen Jing Shogo Ninomiya Shigeyuki Esumi Takaichi Fukuda Wu Shengxi nobuaki tamamaki Department of Human Anatomy Histology and EmbryologyK.K.Leung Brain Research CentreFourth Military Medical University

＜正＞Subventricular zone（SVZ）of the telencephalon and subgranular zone（SGZ）of the hippocampus have been regarded as the main proliferative regions even after *** we report that leptomeninges have neural stem/prog...

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Neuron progenitors in the leptomeninges derived from the ventricular zone during development

Neuron progenitors in the leptomeninges derived from the ven...

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中国神经科学学会第十二届全国学术会议

作者： Jing Chen Jing Huang Fei-Fei Wu Bao-Lin Guo Guo-Hong Cai nobuaki tamamaki Sheng-Xi Wu Department of Anatomy and K.K.Leung Brain Research Centre Fourth Military Medical University Department of Neurobiology and Collaborative Innovation Centre for Brain Science Fourth Military Medical University Department of Morphological Neural Science Graduate School of Medical SciencesKumamoto University

Objective Meninges which surround the center nerve system consist of pia mater, arachnoid membrane and dura mater. Pervious research showed that these membranes work not only for a simple protection but also to keep the brain condition. It is reported that neural progenitors can be activated in the cortical surface adjacent to the injured region and leptomeninges（include the pia mater and arachnoid membrane）, and the cells sorted from the leptomeninges can form neurosphere-like clusters with selfrenewal activity in vitro. All these studies indicated that leptomeninges may be a novel stem cell niche. Some researchers thought that the microvascular pericytes may be the potential sources of neural progenitors. However, here we report that the neural progenitors may be derived from the ventricular zone as other nevour system. Methods The existence of potential neural progenitors in the leptomeninges was identified by different progenitor makers and Brd U label. And the origin of these neural progenitors was shown by in utero electroporation. Results（1） The ENKergic neurons born at E11.5 can be found near the leptomeninges of spinal cord during development stages;（2） Granule cells in the leptomeninges express or transcient express PV, GAD67 and Tbr2 during development;（3） 24 h after electroporation, the GFP-positive cells mainly located near the SVZ, but near the surface of the neocortex, there are also some GFP-positive cells. Conclusion Neural progenitors in the leptomeninges derived from the ventricular zone during development.

关键词： Neural progenitors leptomeninges development ventricular zone

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