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Anti-angiogenic effect of Artocarpus heterophyllus seed methanolic extract in ex ovo chicken chorioallantoic membrane

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 240-244页

作者： Swastika Oktavia nastiti wijayanti Bambang Retnoaji Post Graduate Program Biology FacultyUniversitas Gadjah MadaYogyakartaIndonesia Department of Biology Science and Pharmacy FacultyMathla'ul Anwar UniversityBantenIndonesia Laboratory of Animal Physiology Faculty of BiologyUniversitas Gadjah MadaYogyakartaIndonesia Laboratory of Animal Structure and Development Faculty of BiologyUniversitas Gadjah MadaYogyakartaIndonesia

Objective: To examine the anti-angiogenic potential of Artocarpus heterophyllus(A. heterophyllus) seed extract in chicken chorioallantoic membrane(CAM).Methods: This study used chicken CAM ex ovo culture to examine the potential antiangiogenic activity of A. heterophyllus seed methanolic extract. Basic fibroblast growth factor was used to induce the ectopic formation of blood vessels on CAM treated with extract. Blood vessel number was assessed by macroscopic and microscopic observation,and compared and analyzed for all treatments and ***: Macroscopic observation revealed that a dose of 35 mg/m L of methanolic extract of A. heterophyllus seeds could inhibit basic fibroblast growth factor-induced angiogenesis by 61% in chicken CAM ex ovo culture. This concurred with microscopic observations on the histological structure of blood vessels, which indicated that extract treatment repressed the formation of new blood ***: This is the first study to report the anti-angiogenic effect of methanolic extract derived from A. heterophyllus seeds and its potential as a candidate for future anticancer therapy.

关键词： Angiogenesis Artocarpus heterophyllus CAM Ex ovo culture

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The Analysis of Primer Gene of Phosphodiesterase Type 5 (PDE5) on Erectyle Dysfungction

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Open Journal of Applied Sciences 2018年第9期8卷 398-410页

作者： Theopilus W. Watuguly Indranila K. Samsuria Pudji Astuti Tri Rini Nuringtyas nastiti wijayanti Syahran Wael Department of Biology Pattimura University Maluku Indonesia Department of Clinical Pathology Diponegoro University Semarang Indonesia Department of Veterinary Medicine Gadjah Mada University Yogyakarta Indonesia Department of Biology Gadjah Mada University Yogyakarta Indonesia

Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurological disorders, stroke, diabetes, atherosclerosis, lifestyle, alcohol, smoking, and age-related hormonal decline can cause infertility. The natural treatment of sexual dysfunction through aphrodisiac activity of the plant is to increase sexual hormones, spermatogenesis activity and through PDE5 inhibitors such as sildenafil, verdenafil, and tadalafil which can inhibit the hydrolysis of second messenger cGMP of penis smooth muscle cells. Primer Design for amplification of several PDE5 gene nucleotide sequences obtained from NCBI gen banks and tested directly through explosions at NCBI and also using MEGA 6, primer 3 plus, and fast PCR software. The natural treatment of sexual dysfunction through aphrodisiac activity from plants to increase sexual hormone, spermatogenesis activity and inhibitor PDE5 such as sildenafil, vardenafil, and tadalafil can inhibit hydrolysis second messenger of cells cGMP smooth penis muscles. Method: Primer design stages for several sequences are data supply, multiple sequence alignment, sequence trimming, primer design (fastPCR input), in silico PCR analysis, and primer evaluation (Primer Test, OligoCalc and BLAST). Results: Primer of PDE5 that is choosen is with reverse sequence 5-TGCATTGACCATGTCTCTCGTT-3, forward 5-CGCCGATCTGGGCTGAACTA-3 able to amplify template DNA at temperature 67.2°C, 65.8°C, 63.7°C, however, the DNA band fragment looks not very clear, while it is more clearly seen at Tm temperature 61.2°C, 59.1°C, 57.8°C and 57°C. Conclusion: PDE5 primers can be amplified well at temperature 61.2°C, 59.1°C, 57.8°C and 57°C. PDE5 primer succeeded in amplifying DNA with a product length of 402 bp.

关键词： Erectile Disfunction PDE5 Primer PCR

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Potential of <i>Syzygium aromaticum</i>(Clove) Leaf Extract on Immune Proliferation Response in Balb/c Mice Infected with <i>Salmonella typhimurium</i>

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Case Reports in Clinical Medicine 2018年第12期7卷 613-627页

作者： Syahran Wael Theopilus Wilhelmus Watuguly Ine Arini Alwi Smit Nur Matdoan Dwi Retna Prihati Ana Bina Sari Didik Wahyudi Tri Rini Nuringtyas nastiti wijayanti Pudji Astuti Department of Biology Universitas Pattimura Kota Ambon Indonesia Department of Biology Universitas Gadjah Mada Yogyakarta Indonesia Department of Midwifery Poltekkes Kemenkes Surakarta Klaten Indonesia Department of Medical Diagnostics Bakti Asih College of Health Bandung Indonesia Department of Medical Laboratory Technology National College of Health Solo Indonesia Department of Veterinary Medicine Universitas Gadjah Mada Yogyakarta Indonesia

Background and Objective: Syzygium aromaticum is an immunomodulator with the main active compound eugenol which can stimulate the function of lymphocyte proliferation and macrophage production. Lymphocytes play an important role for body protection against infection. This study aims to analyze the effect of S. aromaticum extract to increase proliferation of lymphocytes, lymphoblasts and macrophage production in Balb/c mice infected with Salmonella typhimurium. Methodology: Mice strain Balb/c were divided into 4 groups: treatment group infected with S. typhimurium and control group (without treatment) to tested the activity of peritoneal macrophages. The treatment group was administrated by S. aromaticum leaf extract (15 mg, 75 mg, 150 mg/kgbw) for 12 days. Results: S. aromaticum leaf extract increased the proliferation activity (p Conclusion: Administration of S. aromaticum leaf extract increased the proliferation activity of lymphocytes, lymphoblasts and ROI secretion of macrophagesin Balb/c mice infected with S. typhimurium.

关键词： Syzygium aromaticum Lymphocytes Lymphoblast Macrophage Salmonella typhimurium

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Adherence to iron supplementation amongst pregnant mothers in Surabaya, Indonesia: Perceived benefits, barriers and family support

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International Journal of Nursing Sciences 2018年第3期5卷 243-248页

作者： Mira Triharini Nursalam Agus Sulistyono Merryana Adriani Ni Ketut Alit Armini Aria Aulia nastiti Nursing Faculty Universitas AirlanggaSurabayaIndonesia Doctoral Student's Health Science of Public Health Faculty Universitas AirlanggaSurabayaIndonesia Dr.Soetomo General Hospital Surabaya Indonesia Public Health Faculty Universitas AirlanggaSurabayaIndonesia

Background:Anaemia during pregnancy is a major nutritional problem that can cause preterm delivery and low birth *** to iron supplementation can prevent anaemia during ***,adherence to iron supplementation remains a problem in many ***:This study aimed to identify the correlations of the perceived benefits and perceived barriers of and family support for iron supplementation with adherence to this practice amongst pregnant woman in Surabaya,***:A cross-sectional study was carried out on 102 pregnant women who attended check-ups at the Puskesmas and received iron *** were collected using ***:Perceived benefits(r=0.334,P=0.001),perceived barriers(r=-0.294,P=0.003)and family support(r=0.263,P=0.008)were noted to be correlated with adherence to iron supplementation amongst pregnant woman in Surabaya,***:Perceived benefits,perceived barriers and family support are related to adherence to iron supplementation;thus,developing good perceptions and family support should be properly promoted.

关键词： Adherence Anaemia Family support Iron Pregnancy

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