检索结果-南通市图书馆

Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2009年第11期52卷 1081-1090页

作者： YAN Bei1, A JiYe1, HAO HaiPing1, WANG GuangJi1, ZHU XuanXuan2, 3, ZHA WeiBin1, LIU LinSheng1, GUAN EnZe3, ZHANG Ying1, GU ShengHua1, HUANG Qing1 & ZHENG YuanTing1 1Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, nanjing 210009, China 2Pharmacological Laboratory of Clinical Research Institute, Jiangsu Provincial Hospital of Traditional Chinese Medicine, nanjing 210029, China 3College of Pharmacy, nanjing University of Traditional Chinese Medicine, nanjing 210029, China Key Laboratory of Drug Metabolism and Pharmacokinetics China Pharmaceutical University Nanjing China Pharmacological Laboratory of Clinical Research Institute Jiangsu Provincial Hospital of Traditional Chinese Medicine Nanjing China College of Pharmacy Nanjing University of Traditional Chinese Medicine Nanjing China

The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".

关键词： Metabonomics GC/TOF-MS heart blood stasis obstruction pattern qi and yin deficiency pattern myocardial ischemia rat model

Adenovirus-mediated overexpression of novel mutated IκBα inhibits nuclear factor κB activation in endothelial cells

在线全文

学校读者我要写书评

暂无评论

中华医学杂志(英文版) 2005年第17期000卷 16-22页

作者： ZHOU Lin-fu, YIN Kai-sheng, ZHU Zi-lu, ZHU Yi, YAO Xin, MAO Hui, XIE Wei-ping and HUANG MaoDepartment of Respiratory Medicine, First Affiliated Hospital, nanjing Medical University, nanjing 210029, ChinaDepartment of Biochemistry and Molecular Biology, nanjing MedicalUniversity, nanjing 210029, China Department of Respiratory Medicine First Affiliated Hospital Nanjing Medical University Nanjing 210029 China Department of Biochemistry and Molecular Biology Nanjing Medical University Nanjing 210029 China

Both effective target gene and efficient gene transfer are critical for gene therapy. Asthma is a chronic airway inflammatory disease involved in a variety of susceptible genes for atopy.~1 Multiple transmembrane signals converge on the nuclear factor κB (NF-κB), a key nuclear factor for maximal transcription of genes associated with immune and inflammatory response.~2 In quiescent cells, NF-κB (p50/p65) is sequestered as inactive trimers in the cytosol via interaction with IκBα, the most important member of the inhibitors of NF-κB (IκB) family, which masks the nuclear localization signals (NLS) and prevents the nuclear transport of NF-κB. It is the phosphorylation of IκBα at serine (Ser) 32/36 residues of N-terminal domain that leads to its subsequent ubiquitination and degradation, resulting in the active NF-κB translocation to the nucleus to initiate transcription of target genes. Hence, IκBα becomes a pivotal candidate to inhibit NF-κB activation for regulation of the NF-κB-dependent genes.~3

关键词： IκBα mutant · nuclear factor κB · recombinant adenovirus · gene therapy · asthma

Proteomic response analysis of endothelial cells of human coronary artery to stimulation with carbachol

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第9期 22-28页

作者： Min YU, Dong-mei CHEN, Gang HU, Hai WANGBeijing Institute of Pharmacology and Toxicology, Beijing 100850, China Department of Pharmacology and Neurobiology, nanjing Medical University, nanjing 210029,China

AIM: To identify the molecular basis of the endothelial target for acetylcholine (ETA). METHODS: Proteomic methods were used to monitor changes in protein expression in the first 10 h following the stimulation of human coronary endothelial cells with carbachol 100 μmol/L. Thirty proteins showing the largest changes were identified by mass spectrometry. RESULTS: Based on analysis with Image Master 2-D

关键词： endothelium carbachol human coronary artery endothelial cells proteome two-dimensional gel electrophoresis protein identification

AT1 receptor in rostral ventrolateral medulla mediating blunted baroreceptor reflex in spontaneously hypertensive rats

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第11期 49-54页

作者： Xin-ya GAO, Feng ZHANG, Ying HAN, Han-jun WANG, Ying ZHANG, Rui GUO, Guo-qing ZHUDepartment of Physiology, nanjing Medical University, nanjing 210029, China

AIM: To determine the role of AT 1 receptor in the rostral ventrolateral medulla (RVLM) in mediating the blunted baroreceptor reflex in spontaneously hypertensive rats (SHR). METHODS: Intravenous injections of graded doses of phenylephrine (1, 5, 10, 20, and 40 μg/kg) increased the blood pressure to elicit the baroreceptor reflex in both SHR and normotensive Wistar rats anesthetized with urethane. The baroreceptor reflex sensitivity (BRS) was determined before and after microinjection of Ang Ⅱ, losartan, or AT1 receptor antisense oligodeoxynucleotides into the RVLM. AT1 receptor protein level in the RVLM was measured by Western blotting. RESULTS: The BRS was significantly decreased in SHR compared with normal rats. Bilateral microinjection of AT1 receptor antagonist losartan (250 nmol/h) into the RVLM partly reversed the blunted BRS in SHR, but had no significant effect on the BRS in normal rats. Ang Ⅱ (1.5 nmol/h) significantly inhibited the BRS in normal rats, which was completely abolished by pretr

关键词： angiotensin Ⅱ antisense oligodeoxynucleotide hypertension baroreceptor reflex rostral ventrolat-eral medulla

Interaction between M-CSF and IL-10 on productions ofIL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第10期 121-125页

作者： Xiao-hui JI, Ting YAO, Jun-chuan QIN, Shu-kui WANG, Hui-juan WANG, Kun YAOMicrobiology and Immunology Institute of nanjing Medical University, nanjing 210029 National Key Laboratory of Medical Pharmaceutical Biotechnology, nanjing University, nanjing 210008 The First Hospital of nanjing Affiliated to nanjing Medical University, nanjing 210006, China

AIM: To Study the interaction of macrophage colony-stimulating factor (M-CSF) and interleukin-10 (IL-10) in productions of IL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes. METHODS: Purified adherent human monocytes were cultured with M-CSF or IL-10 alone, or with M-CSF+IL-10 and 2-3d later, the culture supernatants and cells were separated and collected. IL-12P40 and IL-18 levels in the superna-tants were determined by ELISA and the percentages of CD14, CD23, and CD64 positive cells were examined by flow cytometry. RESULTS: (1) IL-10 decreased M-CSF-induced IL-18 levels, while M-CSF further reduced IL-12P40 level in the culture supernatants of IL-10-treated monocytes; (2) IL-10 alone had no effect on the percentage of CD14-positive cells, but further increased the percentage of CD14-positive cells induced by M-CSF; M-CSF alone had no effect on the percentage of CD64-positive cells, but further increased the percentage of CD64-positive cells induced by IL-10; (3) IL-10 decreased

关键词： macrophage colony-stimulating factor interleukin-10 monocytes cytokines membrane molecule

Computation of Wave Refraction-Diffraction with Error Vector Propagation Method

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

China Ocean Engineering 1994年第4期9卷 401-410页

作者： Zuo Qihua , Yao Guoquan and Ding Bincan Senior Engineer, nanjing Hydraulic Research Institute, nanjing 210029 Professor and Senior Engineer, nanjing Hydraulic Research Institute, nanjing 210029 Engineer, nanjing Hydraulic Research Institute, nanjing 210029

After modifying the basic computation model made by Panchang (1988), the error vector propagation (EVP) method has been adopted to compute the combined effects of water wave refraction and diffraction in the presence of reflection boundary. The results show that the present method is successful in restraining the noise in Panchang's solution. Compared to other numerical methods for the mild-slope wave equation, EVP method can both consider the influence of reflection and save computer memory and computing time.

关键词： water wave refraction and diffraction numerical computation EVP method

Analysis and application of the scale effect of flood discharge atomization model

在线全文

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2011年第S1期54卷 64-71页

作者： WU ShiQiang 1,WU XiuFeng 1,2,ZHOU Hui 1,CHEN HuiLing 1,SHA HaiFei 1 & ZHOU Jie 1 1 State Key Laboratory of Hydrology-Water resources and Hydraulic Engineering,nanjing Hydraulic Research Institute,nanjing 210029,China 2 Hohai University,nanjing 210098,China State Key Laboratory of Hydrology-Water resources and Hydraulic Engineering Nanjing Hydraulic Research Institute Nanjing China Hohai University Nanjing China

The phenomenon of discharge atomization occurs as hydraulic structures discharging,which influences the safety of power station,electrical equipment and produces environmental pollution.A series of physical model tests and feedback analysis are adapted to preliminarily study the scale effect of discharge atomization model by use of the field observation data of discharge *** effect of Re and We numbers of flow on the atomization intensity is analyzed.A conversion relationship of atomization intensity between prototype and model results and the similarity criteria of the atomization range are developed. The conclusion is that the surface tension of discharge atomization model could be ignored when the Weber number is larger than *** case studies are given by use of the similitude criteria of the atomization model.

关键词： discharge atomization scale effect energy dissipation model test feedback analysis

Siltation Prediction for Navigation Channels and Harbour Basins on Muddy Beach (II)

在线全文

学校读者我要写书评

暂无评论

China Ocean Engineering 1992年第3期7卷 297-316页

作者： Liu Jiaju and Zhang Jingchao Professor, Senior Engineer, nanjing Hydraulic Research Institute, 210029 nanjing Senior Engineer, nanjing Hydraulic Research Institute, 210029 nanjing

5. Application and Popularization of Computational Methods for Siltation The above mentioned computational method covers siltation in navigation channels andharbour basins on muddy beach. This part mainly deals with the possibility of its application tosilty beach and sandy beach and the computation of scouring. The following discussion involvestwo aspects, and then some computational examples are given.

关键词： Siltation Prediction for Navigation Channels and Harbour Basins on Muddy Beach II

ANALYSIS OF HUMAN PLACENTA BY ^(31)P NUCLEAR MAGNETIC RESONANCE AND THIN-LAYER CHROMATOGRAPHY SCANNING COMBINED WITH THE CORRECTIVE METHOD OF ABSORBANCE PROPORTIONAL COEFFICIENT

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1993年第12期4卷 1081-1084页

作者： Rong GUO Jian wei CHEN Yi Min XU AiHua ZHANG nanjing College of Traditional Chinese Medicine,nanjing 210029

Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.

关键词： P NUCLEAR MAGNETIC RESONANCE AND THIN-LAYER CHROMATOGRAPHY SCANNING COMBINED WITH THE CORRECTIVE METHOD OF ABSORBANCE PROPORTIONAL COEFFICIENT ANALYSIS OF HUMAN PLACENTA BY LPC TLC