Proteomic response analysis of endothelial cells of human coronary artery to stimulation with carbachol

作     者：Min YU, Dong-mei CHEN, Gang HU, Hai WANGBeijing Institute of Pharmacology and Toxicology, Beijing 100850, China Department of Pharmacology and Neurobiology, Nanjing Medical University, Nanjing 210029,China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第9期

页      面：22-28页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：ProjectsupportedbyaresearchgrantfromStateKeyBasicResearchandDevelopmentfromtheMinistryofScienceandTechnologyofChina(G1998051112) 

主　　题：endothelium carbachol human coronary artery endothelial cells proteome two-dimensional gel electrophoresis protein identification 

摘      要：AIM: To identify the molecular basis of the endothelial target for acetylcholine (ETA). METHODS: Proteomic methods were used to monitor changes in protein expression in the first 10 h following the stimulation of human coronary endothelial cells with carbachol 100 μmol/L. Thirty proteins showing the largest changes were identified by mass spectrometry. RESULTS: Based on analysis with Image Master 2-D