Interaction between M-CSF and IL-10 on productions ofIL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes

作     者：Xiao-hui JI, Ting YAO, Jun-chuan QIN, Shu-kui WANG, Hui-juan WANG, Kun YAOMicrobiology and Immunology Institute of Nanjing Medical University, Nanjing 210029 National Key Laboratory of Medical Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008 The First Hospital of Nanjing Affiliated to Nanjing Medical University, Nanjing 210006, China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第10期

页      面：121-125页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：ProjectsupportedbytheNationalHigh-TechnologyProgramofChina(863-102-11-8)theNaturalScienceFundofEducationDepartment JiangsuProvinceGovernment(98KJB310001) 

主　　题：macrophage colony-stimulating factor interleukin-10 monocytes cytokines membrane molecule 

摘      要：AIM: To Study the interaction of macrophage colony-stimulating factor (M-CSF) and interleukin-10 (IL-10) in productions of IL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes. METHODS: Purified adherent human monocytes were cultured with M-CSF or IL-10 alone, or with M-CSF+IL-10 and 2-3d later, the culture supernatants and cells were separated and collected. IL-12P40 and IL-18 levels in the superna-tants were determined by ELISA and the percentages of CD14, CD23, and CD64 positive cells were examined by flow cytometry. RESULTS: (1) IL-10 decreased M-CSF-induced IL-18 levels, while M-CSF further reduced IL-12P40 level in the culture supernatants of IL-10-treated monocytes; (2) IL-10 alone had no effect on the percentage of CD14-positive cells, but further increased the percentage of CD14-positive cells induced by M-CSF; M-CSF alone had no effect on the percentage of CD64-positive cells, but further increased the percentage of CD64-positive cells induced by IL-10; (3) IL-10 decreased