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Development and application of a multi-step porcine in vitro system to evaluate feedstuffs and feed additives for their efficacy in nutrient digestion, digesta characteristics, and intestinal immune responses

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Animal Nutrition 2024年第2期17卷 265-282页

作者： Hee Yeon kim jun-Ok moon Sung Woo kim Application Center CJ Blossom Park Department of Animal Science North Carolina State University

In vitro model provides alternatives to the use of live animals in research. In pig nutrition, there has been a tremendous increase in in vivo research over the decades. Proper utilization of in vitro models could provide a screening tool to reduce the needs of in vivo studies, research duration, cost, and the use of animals and feeds. This study aimed to develop a multi-step porcine in vitro system to simulate nutrient digestion and intestinal epithelial immune responses affected by feedstuffs and feed additives. Seven feedstuffs(corn, corn distillers dried grains with solubles [corn DDGS], barley, wheat, soybean meal, soy protein concentrates, and Corynebacterium glutamicum cell mass [CGCM]), feed enzymes(xylanase and phytase), and supplemental amino acids(arginine, methionine, and tryptophan), were used in this in vitro evaluation for their efficacy on digestibility, digesta characteristics, and intestinal health compared with the results from previously published in vivo studies. All in vitro evaluations were triplicated. Data were analyzed using Mixed procedure of SAS9.4. Evaluations included(1) nutrient digestibility of feedstuffs,(2) the effects of feed enzymes, xylanase and phytase, on digestibility of feedstuffs and specific substrates, and(3) the effects of amino acids, arginine, tryptophan, and methionine, on anti-inflammatory, anti-oxidative, and anti-heat stress statuses showing their effects(P < 0.05) on the measured items. Differences in dry matter and crude protein digestibility among the feedstuffs as well as effects of xylanase and phytase were detected(P < 0.05), including xylo-oligosaccharide profiles and phosphorus release from phytate. Supplementation of arginine, tryptophan, and methionine modulated(P < 0.05) cellular inflammatory and oxidative stress responses. The use of this in vitro model allowed the use of 3 experimental replications providing sufficient statistical power at P < 0.05. This indicates in vitro models can have increased prec

关键词： Digestion Feed additive Feedstuff In vitro Intestine Pig

Voxelated opto-physically unclonable functions via irreplicable wrinkles

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Light(Science & Applications) 2023年第11期12卷 2378-2390页

作者： Kitae kim Se-Um kim moon-Young Choi Mohsin Hassan Saeed Youngmin kim jun-Hee Na Department of Convergence System Engineering Chungnam National University99 Daehak-roYuseong-guDaejeon 34134Republic of Korea Department of Electrical and Information Engineering Seoul National University of Science&Technology232 Gongneung-roNowon-guSeoul 01811Republic of Korea Department of Electrical Electronicsand Communication Engineering EducationChungnam National University99 Daehak-roYuseong-guDaejeon 34134Republic of Korea Hologram Research Center Korea Electronics Technology InstituteWorld Cup buk-ro 54-gilMapo-guSeoul 03924Republic of Korea

The increased prevalence of the Internet of Things(IoT)and the integration of digital technology into our daily lives have given rise to heightened security risks and the need for more robust security *** response to these challenges,physical unclonable functions(PUFs)have emerged as promising solution,offering a highly secure method to generate unpredictable and unique random digital values by leveraging inherent physical ***,traditional PUFs implementations often require complex hardware and circuitry,which can add to the cost and complexity of the *** present a novel approach using a random wrinkles PUF(rw-PUF)based on an optically anisotropic,facile,simple,and cost-effective *** wrinkles contain randomly oriented liquid crystal molecules,resulting in a two-dimensional retardation map corresponding to a complex birefringence ***,our proposed technique allows for customization based on specific requirements using a spatial light modulator,enabling fast *** random wrinkles PUF has the capability to store multiple data sets within a single PUF without the need for physical ***,we introduce a concept called‘polyhedron authentication,’which utilizes three-dimensional information storage in a voxelated random wrinkles *** approach demonstrates the feasibility of implementing high-level security technology by leveraging the unique properties of the rw-PUF.

关键词： hardware functions utilize

Fingerprint inspired advanced end-effector and its applications

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Friction 2022年第10期10卷 1739-1750页

作者： Sung Ho LEE Hyo Sung kim Han jun PARK moon Kyu KWAK Department of Electrical Engineering and Computer Science University of MichiganAnn Arbor 48109USA Department of Mechanical Engineering Kyungpook National UniversityDaegu 41566Republic of Korea

The human fingertip consists of a fingerprint with many micro-grating *** main roles of the fingerprint could be divided into two purposes,namely,the enhancement of the frictional force and the effective transmittance of the *** this study,we present the fingerprint-inspired end-effector that has not only admirable frictional force but also electric *** end-effector is composed of fluorocarbon rubber,one of the famous materials to achieve high frictional force and *** various experiments,the novel performance of micro structured fluorocarbon rubber end-effector(MSFE)is characterized by comparing with a macroscale patterned sample(MPS),which has been already used in real industrial *** results are analyzed ***,as feasible applications,we suggest two applications based on the role of the *** is the conductive astronaut glove with high frictional force,and the other one is a non-slip pad for the next-generation glass transfer *** these experiments,we successfully observe the enhanced system performance and confirm the possibility of using the MSFE as feasible *** believe that the MSFE could be a useful and powerful alternative as an end-effector,not only in the aerospace industry but also in display manufacturing processes.

关键词： fingerprint end-effector fluorocarbon rubber(FKM) glass transfer system spacesuit

Electrical resistivity evolution in electrodeposited Ru and Ru-Co nanowires

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Journal of Materials Science & Technology 2022年第10期105卷 17-25页

作者： jun Hwan moon Seunghyun kim Taesoon kim Yoo Sang Jeon Yanghee kim Jae-Pyoung Ahn Young Keun kim Department of Materials Science and Engineering Korea UniversitySeoul 02841Republic of Korea Institute of Engineering Reserch Korea UniversitySeoul 02481Republic of Korea Advanced Analysis Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea Research Resources Division Korea Institute of Science and TechnologySeoul 02792Republic of Korea

Nanoscale ruthenium(Ru)-based materials are promising replacements for existing multilayered Cu interconnects in integrated ***,it is not easy to apply the results of previously reported studies directly to the electrochemical damascene process because the previous studies have mainly focused on thin flms by dry ***,we report the electrical resistivity and microstructure of electrodeposited Ru *** estimate that the resistivity value of a 10 nm diameter Ru nanowire to be71.6μΩcm after analyzing the resistivity values of individual nanowires with various ***,we investigate the electrical properties of Ru_(x)Co_(1-x)nanowires where x is 0.04–0.99 at.%as possible replacements of the current Ta N barrier *** the entire composition range,the resistivity values of alloys are much lower than that of the conventional Ta ***,Ru and Ru-alloy nanowires surrounded by dielectric silica are thermally stable after 450°C heat ***,the nanoscale Ru and Ru-Co alloys possessing low resistivity values can be candidates for the interconnect and barrier materials,respectively.

关键词： Ruthenium(Ru) Ruthenium-cobalt(Ru-Co) Nanowire Electrical resistivity Microstructure Electrodeposition

Preparation of glucosamine by hydrolysis of chitosan with commercial α-amylase and glucoamylase

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第11期12卷 931-934页

作者： Sai-kun PAN Sheng-jun WU Jin-moon kim Jiangsu Key Laboratory of Marine Biotechnology Huaihai Institute of Technology Lianyungang 222005 China School of Marine Science and Technology Huaihai Institute of Technology Lianyungang 222005 China Food Safety and Inspection Service Office of Field Operations US Department of Agriculture Albany iVY 12203 USA

Objective： In order to overcome the defects of chemical hydrolysis approach to prepare glucosamine, an enzymatic hydrolysis method was developed. Methods： Glucosamine was prepared by hydrolyzing chitosan, em- ploying e-amylase initially, and subsequently, glucoamylase. Results： The optimal hydrolyzing conditions were as follows： reaction time, 4 h; pH, 5.0; temperature, 50 ℃; and, α-amylase, 80 U/g for the initial reaction. Subsequently, glucoamylase was added in the presence of a-amylase. The optimal reaction conditions were found to be： reaction time, 8 h; pH, 4.5; temperature, 55 ℃; and, glucoamylase, 4000 U/g. The hydrolysates were subject to filtrating, concentrating to about 20% （w/w）, precipitating with five volumes of ethanol, and drying at 60 ℃ for 2 h. The content and the yield of glucosamine in the dried precipitate were 91.3% （w/w） and 86.2% （w/w）, respectively. Conclusions： The method developed in this study is a promising option in the preparation of glucosamine.

关键词： Glucosamine o-Amylase Glucoamylase Hydrolysis

Environmentally friendly hybrid coating prepared by electro-deposition method at various seawater conditions

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中国有色金属学会会刊：英文版 2009年第B09期19卷 110-113页

作者： Myeong-Hoon LEE Kyung-Man moon Jong-Do kim Kang jun Kwang-Ho kim Division of Marine System Engineering Korea Maritime University Dongsam-Dong Youngdo Busan 606-791 Korea National Core Research Center for Hybrid Materials Solution Pusan National University Busan 609-735 Korea

The formation mechanism of calcareous deposit films in seawater involves an increase in pH at the metal/solution interface due to cathodic reactions, a raised carbonate ion concentration at the interface and precipitation of inorganic deposits such as CaCO3, Mg(OH)2. Environmentally friendly hybrid calcareous deposit films were formed by an electrochemical technique on steel substrates in various solution environments. And the influence of dissolved gas on formation of calcareous deposit films was investigated by scanning electron microscopy(SEM), energy dispersive spectroscopy(EDS) and X-ray diffractometry(XRD). Consequently, these results showed that formation of good overall calcareous deposited films by dissolved gas in seawater environments can be achieved by controlling the material composition and structure through effective use of electrochemical method.

关键词：海水混合环保电沉积扫描电子显微镜涂层制备溶液界面

Evolution of systemic therapy for advanced-stage hepatocellular carcinoma

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Hepatobiliary Surgery and Nutrition 2022年第6期11卷 899-902页

作者： moon Haeng Hur Yoon jun kim Department of Internal Medicine and Liver Research Institute Seoul National University College of MedicineSeoulKorea

Since the introduction of sorafenib in 2007(1),a tyrosine kinase inhibitor(TKI),there have been notable advancements in systemic therapy for advanced-stage hepatocellular carcinoma(HCC).Although the European Association for the Study of the Liver(EASL)Clinical Practice Guidelines regarding the management of HCC was published in 2018(2),recent progress,including the advent of several immune checkpoint inhibitors(ICIs),necessitated the revision of this ***,the EASL published a position paper to address the additional improvements on systemic therapy for HCC(3).Here,we aimed to summarize this position paper and comment on some updated points.

关键词： hepatocellular Liver Clinical

High-temperature Oxidation Behavior of Zircaloy-4 and Zirlo in Steam Ambient

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Journal of Materials Science & Technology 2010年第9期26卷 827-832页

作者： Hyung Hoon kim jun Ho kim Jin Young moon Ho Seong Lee Jeong Joo kim Young Suck Chai School of Materials Science and Engineering Kyungpook National University School of Mechanical Engineering Yeungnam University214-1 Dae-dongGyeongsan-si

The oxidation characteristics for Zircaloy-4 and Zirlo in the temperature range of 700-1200°C under steam supply condition were investigated by using a modified thermo-gravimetric *** specimens were oxidized for 3600 s at each temperature and then quenched in a *** oxidation rate constants were measured from the weight gains to evaluate the oxidation behavior in Zircaloy-4 and *** weight gain rates of Zirlo were lower than those of Zircaloy-4,leading to the low rate *** different oxidation behaviors between both cladding materials were considered to be due to the difference in their chemical compositions.

关键词： Zircaloy-4 Zirlo Oxidation

Aqueous levels of erythropoietin in acute retinal vein occlusion with macular edema

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International Journal of Ophthalmology(English edition) 2014年第3期7卷 501-506页

作者： Hyun Jin Shin Hyung Chan kim jun Woong moon Department of Ophthalmology Konkuk University Medical Center Konkuk University School of Medicine Gongdeok Seoul Eye Clinic

AIM:To investigate the aqueous erythropoietin(EPO)levels and associated factors in patients with acute retinal vein occlusion(RVO).METHODS:The aqueous EPO level was measured in patients with macular edema(ME)secondary to acute branched retinal vein occlusion(BRVO)or central retinal vein occlusion(CRVO).Aqueous fluid from cataract patients served as the *** also evaluated whether aqueous level of EPO was associated with factors such as serum EPO level,non-perfusion area,central macular thickness(CMT),and arterio-venous(AV)transit timeRESULTS:Twenty-seven RVO patients(16 BRVO,11CRVO)and 9 control subjects were enrolled in the *** aqueous EPO level(mU/mL)was higher in RVO(68.2±54.3)than that in the control subjects(12.9±5.9).More specifically,the aqueous EPO level was higher in CRVO(118.9±52.1)than that in BRVO(33.3±10.8).However,no differences were found in serum EPO levels among three *** in RVO patients had a positive correlation with the aqueous EPO level(r=0.66).Also,in terms of non-perfusion area,the aqueous EPO levels were more elevated in the ischemic subgroup than in the non-ischemic subgroup in both BRVO and ***:Aqueous EPO levels are elevated in patients with macular edema secondary to recent onset *** with CRVO have higher EPO levels than those with *** aqueous EPO level in RVO has a positive correlation with CMT and is associated with non-perfusion *** results suggest that the aqueous EPO level could be associated with retinal ischemia and may be involved in the pathogenesis of macular edema secondary to RVO.

关键词： aqueous level of erythropoietin acute retinal vein occlusion retinal ischemia macular edema