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Curcumin inhibits proliferation of human lens epithelial cells:a proteomic analysis

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第5期13卷 402-407页

作者： Yan-hong HU Xiu-rong HUANG ming-xin qi Bu-yuan HOU Department of Ophthalmology the Second Affiliated Hospital of Fujian University of Traditional Chinese MedicineFuzhou 350003China Research Center of Pathophysiology Fujian University of Traditional Chinese MedicineFuzhou 350003China

Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction (PCR).

关键词： Curcumin After-cataract Posterior capsular opacification (PCO) Proteomics Lens epithelial cells

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Mitochondrial proteomic analysis of ecdysterone protection against oxidative damage in human lens epithelial cells

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International Journal of Ophthalmology(English edition) 2014年第1期7卷 38-43页

作者： Chun-Yan Feng Xiu-Rong Huang ming-xin qi Song-Wen Tang Sheng Chen Yan-Hong Hu Fa-Jie Ke xin Wang Department of Ophthalmology Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine Research Center of Pathophysiology Fujian University of Traditional Chinese Medicine Department of Laboratory Fujian University of Traditional Chinese Medicine subsidiary Rehabilitation Hospital

AIM: To investigate the protective effects of the natural medicinal monomer ecdysterone(ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3(HLE-B3) caused by hydrogen peroxide 21(H2 O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. · METHODS: HLE-B3 cells were treated with H2O2(300μmol/L),β-estuarial(E2; 10-8mol/L) and H2 O2,ECR(10-6mol/L) and H2 O2,or left untreated. Altered expression of all mitochondrial proteins was analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS). The mass/charge(M/Z) ratios of each peak were tested by the Kruskal-Wallis rank sum test,and the protein peak value of the M/Z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. ·RESULTS: H2O2 up-regulated expression of two protein spots(with M/Z of 6 532 and 6 809). When E2 mitigated the oxidative damage,the expression of one protein spot(M/Z 6 532) was down-regulated. In contrast,ECR downregulated both of protein spots(M/Z 6 532 and 6 809). · CONCLUSION: ECR could effectively inhibite H2O2 induced oxidative damage in HLE-B3 cells. The protein spot at M/Z of 6 532 might be the target spot of ECR against oxidative damage induced by H2 O2.

关键词： ecdysterone mitochondrial proteomics lens epithelial cell senile cataract

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Effects of Dendrobium candidum polysaccharides on microRNA-125b and mitogen-activated protein kinase signaling pathways in diabetic cataract rats

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Traditional Medicine Research 2021年第5期6卷 43-48页

作者： Zi-Yang Chen qian Lan Sheng Chen Yan-Hong Hu Fa-Jie Ke Jun Hu Chun-Yan Feng ming-xin qi Xiu-Rong Huang Comprehensive Clinic of Fujian Academy of Chinese Medicine Fuzhou 350003China Fujian University of Traditional Chinese Medicine Fuzhou 350122China Department of Opthalmology The Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityFuzhou 350003China

Background:Diabetic cataract is a common complication and a lens disorder in ***,Dendrobium officinale polysaccharide(DOP)is found to alleviate diabetes ***,microRNA-125b and mitogen-activated protein kinase signaling pathways play significant roles in ***,the mechanism and the effect of DOP on diabetic cataract remains ***:Wistar male rats were randomly assigned to the control,model,and DOP *** was induced with ***,DOP was orally given for 12 *** Lens Opacities Classification System III was used to evaluate lens opacity by slit-lamp *** lens was then harvested for testing the mRNA expression of microRNA-125b,ERK1,ERK2,Raf and Ras using reverse transcription-polymerase chain *** protein expression of ERK1,ERK2,Raf,and Ras was detected using the Western blot *** targets of microRNA-125b were predicted in miRWalk *** targets were obtained from the Kyoto Encyclopedia of Genes and Genomes ***:The lens opacities of the rats in the control group were almost at ***,the majority of the lens opacities in the model group were C3 to C4 and N1 to N2,and were mostly at C1 to C2 and N0 to N1 in the DOP *** difference was statistically significant(P<0.05).Furthermore,the microRNA-125b expression in the model group is significantly higher compared with the control ***,the microRNA-125b expression in the DOP group is significantly decreased(all P<0.05).The mRNA expression of ERK1,ERK2,Raf,and Ras in the model groups were upregulated compared with those of the control ***,the ERK1 and Raf mRNA expressions of the DOP group were lower compared with the model group(all P<0.05).The protein expression of ERK1,Raf,and Ras in the model group was significantly increased compared with those of the control *** protein expression of ERK1,Raf,and Ras in the DOP group was significantly lower compared with

关键词： Dendrobium officinale polysaccharide microRNA-125b mitogen-activated protein kinase diabetic cataract

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Nerve conduction studies in patients with dermatomyositis or polymyositis

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Chinese Medical Journal 2010年第5期123卷 523-526页

作者： WANG Yue CUI Li-ying CHEN Lin LIU ming-sheng qi xin LI Ben-hong DU Hua Department of Neurology Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 China

Background Involvement of peripheral nerves in dermatomyositis （DM） and polymyositis （PM） is less well known. In the present study we retrospectively analyzed the clinical and electrophysiological records of hospital inpatients with a diagnosis of DM or PM to investigate the association of DM/PM and peripheral neuropathy. Methods The data of inpatients diagnosed with DM or PM were collected in Peking Union Medical College Hospital, and 186 patients （118 patients with DM and 68 with PM） were retrospectively analyzed. Nerve conduction studies （NCSs） of the median nerve, ulnar nerve, posterior tibial nerve, and common peroneal nerve were examined simultaneously. Results There were 71 （38.2%） patients with abnormal NCS findings, 37 （19.9%） with pure motor involvement （decreased compound muscle action potential, CMAP）, and 34 （18.3%） with peripheral neuropathy. Of the 34 peripheral neuropathy patients, 14 （7.5%） had polyneuropathy, 1 （0.5%） had multiple mononeuropathy, 16 （8.6%） had carpal tunnel syndrome （CTS）, 1 （0.5%） had trigeminal sensory neuropathy, 1 （0.5%） had ulnar sensory neuropathy, and 1 （0.5%） had brachial plexus involvement. The prevalence of malignant disease （3/34, 8.8%）, other connective tissue diseases （CTDs）（7/34, 20.6%） and diabetes （6/34, 17.6%） was significantly greater in DM/PM patients with peripheral neuropathy （X2=15.855, P=0.000） compared with DM/PM patients without involvement of peripheral nerves （5/115, 4.3% and 7/115, 6.1%, respectively）. Conclusions Peripheral neuropathy in DM/PM often suggests a complication with cancer, other CTDs, diabetes or CTS. From a practical point of view, NCS for DM/PM may help find the underlying disorders.

关键词： dermatomyositis polymyositis nerve conduction study

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Curcumin inhibits proliferation of human lens epithelial cells： a proteomic analysis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第2期14卷 86-86页

作者： Yan-hong HU Xiu-rong HUANG ming-xin qi Bu-yuan HOU

Objective： The incidence of after-cataracts [also known as posterior capsular opacification （PCO）] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells （LECs） is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the in- hibitory effects of curcumin （Cur） on the proliferation of human lens epithelial B3 （HLE-B3） cells. Methods： Recombinant human basic fibroblast growth factor （rhbFGF） was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results： We found that the absorbance （,4） value ofrhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower compared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF, Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge （m/z） ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions： These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of riLE-B3 cell proliferation. Cur may be a reliable and effective drug for prevention and treatment of polymerase chain reaction （PCR）.

关键词：晶状体上皮细胞蛋白质组分细胞增殖姜黄素重组人碱性成纤维细胞生长因子白内障手术 CO2培养箱聚合酶链反应

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Curcumin inhibits proliferation of human lens epithelial cells:a proteomic analysis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第12期13卷 1005-1005页

作者： Yan-hong HU,Xiu-rong HUANG,ming-xin qi,Bu-yuan HOU Department of Ophthalmology the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine Research Center of Pathophysiology Fujian University of Traditional Chinese Medicine

Objective:The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract *** the proliferation of lens epithelial cells (LECs) is a primary goal in preventing ***,we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) ***:Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells,which were incubated with 20 mg/L Cur in a CO 2 incubator for 24 ***:We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control ***,the A value of the Cur group was significantly lower compared to the rhbFGF group,with an inhibition of 53.7%.Five different protein spots were obtained from proliferative HLE-B3 cells induced by *** different protein spots were obtained in HLE-B3 cells incubated with *** were the common variational protein spots at mass/charge (m/z) ratios of 8093 and 13767 between rhbFGF group and control group as well as between the Cur group and rhbFGF ***:These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by *** protein spots at m/z of 8093 and 13767 may be the targets of Cur-induced inhibition of HLE-B3 cell *** may be a reliable and effective drug for prevention and treatment of polymerase chain reaction (PCR).

关键词：晶状体上皮细胞蛋白质组分细胞增殖姜黄素重组人碱性成纤维细胞生长因子白内障手术 CO2培养箱聚合酶链反应

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TLR4和CD14在体外培养的晶状体上皮细胞有表达

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国际眼科杂志 2010年第4期10卷 630-632页

作者：黄秀榕祁明信王军福建中医药大学病理生理研究中心中国福建省福州市350003 福建中医药大学附属第二人民医院眼科中国福建省福州市350003

目的:探讨Toll样受体4(toll-like receptor4,TLR4)和CD14在晶状体上皮细胞(lens epithelial cell,LEC)的表达。方法:采用一步法反转录多聚酶链反应(polymerase chain reaction,PCR)检测LEC中TLR4mRNA和CD14mRNA的表达。结果:用RT-PCR检测分别获得451bp和348bp的清晰扩增条带,经基因测序证实为TLR4 mRNA和CD14 mRNA。结论:晶状体上皮细胞可表达TLR4和CD14。

关键词： Toll样受体4 CD14 表达晶状体上皮细胞

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凉血化瘀法干预老年性黄斑变性CNV与外周血CCR3表达的相关研究

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国际眼科杂志 2013年第3期13卷 546-548页

作者：胡艳红祁明信陈胜福建中医药大学附属第二人民医院中国福建省福州市350003

目的:探讨凉血化瘀法治疗渗出型老年性黄斑变性CNV与外周血T淋巴细胞及嗜酸性粒细胞表面CCR3表达的关系。方法:以32例38眼渗出型老年性黄斑变性患者、44例73眼萎缩型老年性黄斑变性患者及50例健康人为观察对象,流式细胞术检测外周血T淋... 详细信息

目的:探讨凉血化瘀法治疗渗出型老年性黄斑变性CNV与外周血T淋巴细胞及嗜酸性粒细胞表面CCR3表达的关系。方法:以32例38眼渗出型老年性黄斑变性患者、44例73眼萎缩型老年性黄斑变性患者及50例健康人为观察对象,流式细胞术检测外周血T淋巴细胞及嗜酸性粒细胞表面CCR3的表达,对渗出型老年性黄斑变性患者予凉血化瘀中药治疗,比较治疗前后患者外周血CCR3的表达。结果:渗出型老年性黄斑变性患者外周血T淋巴细胞及嗜酸性粒细胞表面CCR3表达均高于健康对照组(P0.05)。渗出型老年性黄斑变性患者治疗前后外周血CCR3的表达没有显著性差异(P>0.05)。结论:渗出型老年性黄斑变性CNV的形成可能与外周血T淋巴细胞及/或嗜酸性粒细胞表面CCR3的高表达有关,而凉血化瘀法能有效治疗渗出型老年性黄斑变性CNV,却与CCR3表达不相关。

关键词：老年性黄斑变性脉络膜新生血管 CCR3 凉血化瘀法

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Influence of gut bacteria on type 2 diabetes:Mechanisms and therapeutic strategy

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World Journal of Diabetes 2025年第1期16卷 27-33页

作者： Xue Wen Lu-ming qi Kui Zhao College of Health and Rehabilitation Chengdu University of Traditional Chinese MedicineChengdu 610000Sichuan ProvinceChina College of Materials and Chemical Engineering Southwest Forestry UniversityKunming 650224Yunnan ProvinceChina

The onset and progression of type 2 diabetes mellitus(T2DM)are strongly associated with imbalances in gut bacteria,making the gut microbiome a new potential therapeutic *** commentary examines the recent publication in World Journal of *** article explores the association between T2DM and gut microbiota,with a focus on the pathophysiological changes related to *** proposes innovative microbiome-targeted therapeutic strategies and evaluates the challenges and future directions of such *** editorial summarizes the key points of their discussion of the role of the gut microbiome in T2DM and elaborates on the influence of specific gut microbial species on the disease through the host–microbiota metabolic *** provides new insights for future research on gut-microbiota-based interventions for T2DM.

关键词： Type 2 diabetes Intestinal microbiome Intestinal axis Biological pathways Treatment Short-chain fatty acids

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蜕皮甾酮和异补骨脂素在HLEC中抗氧化损伤的信号转导机制

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国际眼科杂志 2012年第11期12卷 2072-2074页

作者：张可丽黄秀榕祁明信郭娜江西医学院上饶分院病理教研室中国江西省上饶市334000 福建中医药大学病理生理研究中心中国福建省福州市350003 福建中医药大学附属第二人民医院眼科中国福建省福州市350003

目的:研究异补骨脂素(Isopsoralen,ISR)和蜕皮甾酮(Ecdysterone,ECR)对经H2O2处理的HLEC内磷酸化ERK(p-ERK)的表达,从而探讨雌二醇(β-Estradiol,E2),ECR和ISR是否通过启动或干预ERK/MAPK信号转导途径来发挥抗氧化损伤作用,以揭示E2和ECR及ISR抗氧化损伤的信号转导机制。方法:本研究采用ECR和ISR与人晶状体上皮细胞(HLEC)共同孵育,以E2作为阳性对照,再用H2O2对HLEC造成氧化损伤后,采用流式细胞术(flow cytometer,FCM)检测不同时段的p-ERK蛋白表达。结果:正常HLEC内存在p-ERK表达;H2O2组p-ERK表达量随时间的延长而减少;E2和ECR与ISR各组p-ERK表达量分别在1,6,12h达到高峰。结论:E2和ECR与ISR的抗氧化损伤作用可能是与ERK/MAPK信号通路有关。

关键词：人晶状体上皮细胞雌激素受体异补骨脂素蜕皮甾酮信号转导

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