Effects of Dendrobium candidum polysaccharides on microRNA-125b and mitogen-activated protein kinase signaling pathways in diabetic cataract rats
作者机构：Comprehensive Clinic of Fujian Academy of Chinese MedicineFuzhou 350003China Fujian University of Traditional Chinese MedicineFuzhou 350122China Department of OpthalmologyThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityFuzhou 350003China
出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)
年 卷 期：2021年第6卷第5期
页 面：43-48页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by Fujian Provincial Natural Science Foundation Project(No.2019J01483) Fujian Provincial Health and Health Commission Medical Innovation Project(No.2018-CXB-15)of China
主 题：Dendrobium officinale polysaccharide microRNA-125b mitogen-activated protein kinase diabetic cataract
摘 要：Background:Diabetic cataract is a common complication and a lens disorder in ***,Dendrobium officinale polysaccharide(DOP)is found to alleviate diabetes ***,microRNA-125b and mitogen-activated protein kinase signaling pathways play significant roles in ***,the mechanism and the effect of DOP on diabetic cataract remains ***:Wistar male rats were randomly assigned to the control,model,and DOP *** was induced with ***,DOP was orally given for 12 *** Lens Opacities Classification System III was used to evaluate lens opacity by slit-lamp *** lens was then harvested for testing the mRNA expression of microRNA-125b,ERK1,ERK2,Raf and Ras using reverse transcription-polymerase chain *** protein expression of ERK1,ERK2,Raf,and Ras was detected using the Western blot *** targets of microRNA-125b were predicted in miRWalk *** targets were obtained from the Kyoto Encyclopedia of Genes and Genomes ***:The lens opacities of the rats in the control group were almost at ***,the majority of the lens opacities in the model group were C3 to C4 and N1 to N2,and were mostly at C1 to C2 and N0 to N1 in the DOP *** difference was statistically significant(P0.05).Furthermore,the microRNA-125b expression in the model group is significantly higher compared with the control ***,the microRNA-125b expression in the DOP group is significantly decreased(all P0.05).The mRNA expression of ERK1,ERK2,Raf,and Ras in the model groups were upregulated compared with those of the control ***,the ERK1 and Raf mRNA expressions of the DOP group were lower compared with the model group(all P0.05).The protein expression of ERK1,Raf,and Ras in the model group was significantly increased compared with those of the control *** protein expression of ERK1,Raf,and Ras in the DOP group was significantly lower compared with
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