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Construction of the Seed-Coat cDNA Microarray and Screening of Differentially Expressed Genes in Barley

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 695-700页

作者： Jin-Song PANG*, meng-yuan HE, and Bao liu Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China Laboratory of Molecular Epigenetics Institute of Genetics and Cytology Northeast Normal UniversityChangchun 130024 China

Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, to study this question. Six-thousand and forty-eight clones from the wild type Morex testa+pericarp cDNA library were amplified using PCR, and the DNA fragments were spotted on commercial amino-modified glass slide as microarray. The mRNAs from the developing seed coat (8–15 days) of both the mutant and the wild-type barley plants were isolated, and labeled respectively with Cy3-dUTP and Cy5-dUTP when reversely trans- cribed to cDNAs. The labeled cDNAs were used as probes, mixed at the same molar concentration, and hybridized with the DNA fragments on the slide. Seventy clones exhibiting marked differential expression (ratio>4) were identified from the microarray. All the 25 cDNA clones that showed an over-expression in wild type in comparison to the mutant ant13-152 were sequenced. It was found that most of these over- expressing clones were transcription/translation and hordein-associated genes. These results have laid a solid material basis for further elucidation of the metabolic pathway in proanthocyanidin synthesis in barley and likely other plants.

关键词： barley seed coat proanthocyanidin cDNA microarray

Mechanism of ELL-associated factor 2 and vasohibin 1 regulating invasion,migration,and angiogenesis in colorectal cancer

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World Journal of Gastroenterology 2023年第24期29卷 3770-3792页

作者： Ming-Liang Feng Ming-Jun Sun Bo-Yang Xu meng-yuan liu Hui-Jing Zhang Can Wu Department of Endoscopy The First Hospital Affiliated to China Medical UniversityShenyang 110001Liaoning ProvinceChina

BACKGROUND As a novel endogenous anti-angiogenic molecule, vasohibin 1(VASH1) is not only expressed in tumor stroma, but also in tumor tissue. Moreover, studies have shown that VASH1 may be a prognostic marker in colorectal cancer(CRC). Knockdown of VASH1 enhanced transforming growth factor-β1(TGF-β1)/Smad3 pathway activity and type Ⅰ/Ⅲ collagen production. Our previous findings suggest that ELL-associated factor 2(EAF2) may play a tumor suppressor and protective role in the development and progression of CRC by regulating signal transducer and activator of transcription 3(STAT3)/TGF-β1 signaling pathway. However, the functional role and mechanism of VASH1-mediated TGF-β1 related pathway in CRC has not been *** To investigate the expression of VASH1 in CRC and its correlation with the expression of EAF2. Furthermore, we studied the functional role and mechanism of VASH1 involved in the regulation and protection of EAF2 in CRC cells in *** We collected colorectal adenocarcinoma and corresponding adjacent tissues to investigate the clinical expression of EAF2 protein and VASH1 protein in patients with advanced CRC. Following, we investigated the effect and mechanism of EAF2 and VASH1 on the invasion, migration and angiogenesis of CRC cells in vitro using plasmid *** Our findings indicated that EAF2 was down-regulated and VASH1 was upregulated in advanced CRC tissue compared to normal colorectal tissue. KaplanMeier survival analysis showed that the higher EAF2 Level group and the lower VASH1 Level group had a higher survival rate. Overexpression of EAF2 might inhibit the activity of STAT3/TGF-β1 pathway by up-regulating the expression of VASH1, and then weaken the invasion, migration and angiogenesis of CRC *** This study suggests that EAF2 and VASH1 may serve as new diagnostic and prognostic markers for CRC, and provide a clinical basis for exploring new biomarkers for CRC. This study complements the mechanism of EAF2

关键词： ELL-associated factor 2 Vasohibin 1 Transforming growth factor-β1 Signal transducer and activator of transcription 3 Colorectal cancer Angiogenesis

Sex modification of the association of the radial augmentation index and incident hypertension in a Chinese community-based population

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Journal of Geriatric Cardiology 2021年第1期18卷 39-46页

作者： Qiao QIN Fang-Fang FAN meng-yuan liu Bo liu Jia JIA Long ZHANG Yu-Xi LI Yi-meng JIANG Peng-Fei SUN Dan-Mei HE Jian-Ping LI Ming CHEN Bo ZHENG Yan ZHANG Department of Cardiology Institute of Cardiovascular DiseasePeking University First HospitalBeijingChina

BACKGROUND Arterial stiffness,as assessed by aortic ultrasound and pulse wave velocity,is associated with incident ***,there is still no consensus on whether the augmentation index(AI)affects new onset of *** study investigated the relationship of radial AI(r AI)and incident hypertension in a Chinese community-based population without hypertension at *** A total of 1,615 Chinese non-hypertensive participants from an atherosclerosis cohort in Beijing,China were included in our *** r AI normalized to heart rate of 75 beats/min(r AIp75)was obtained using HEM-9000 ***-onset hypertension was defined as blood pressure≥140/90 mm Hg or self-reported hypertension or taking anti-hypertensive medications at the follow up *** regression models were used to evaluate the impact of r AIp75 on the risk of new-onset *** After a mean 2.35-year follow-up,213(13.19%)participants developed incident *** significant relation between r AIp75 and incident hypertension was observed in the whole population after adjustment for possible confounders(adjusted odds ratio(OR)and 95%confidence interval(CI):1.09[0.95-1.27];P=0.2260).However,r AIp75 was significantly associated with incident hypertension in women,but not in men(adjusted OR and 95%CI:1.29[1.06-1.56],P=0.0113 for women;0.91[0.72-1.15],P=0.4244 for men;P for interaction=0.0133).CONCLUSIONS Sex modified the effect of the r AI on incident hypertension in a Chinese,community-based,non-hypertensive *** of the r AI could be considered in women with a high risk of hypertension for the purpose of primary intervention.

关键词： Sex modification of the association of the radial augmentation index and incident hypertension in a Chinese community-based population

Overexpression of ELL-associated factor 2 suppresses invasion,migration,and angiogenesis in colorectal cancer

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World Journal of Gastrointestinal Oncology 2022年第10期14卷 1949-1967页

作者： Ming-Liang Feng Can Wu Hui-Jing Zhang Huan Zhou Tai-Wei Jiao meng-yuan liu Ming-Jun Sun Department of Endoscopy The First Hospital Affiliated to China Medical UniversityShenyang 110001Liaoning ProvinceChina

BACKGROUND The androgen responsive gene,ELL-associated factor 2(EAF2),expressed in benign prostate tissues,has been shown to play an important role in tumor suppression in a variety of malignant *** addition,some scholars found that EAF2 frameshift mutations are associated with intratumor heterogeneity in colorectal cancer(CRC)and inactivation of EAF2 in microsatellite instability-high ***,the molecular mechanism by which EAF2 is involved in CRC invasion and metastasis remains *** To determine the clinical value of expression of EAF2 protein in CRC,and to study the effects of EAF2 on the invasion,migration,and angiogenesis of CRC cells in *** In this study,we collected colorectal adenocarcinoma and corresponding adjacent tissues to investigate the clinical expression of EAF2 protein in patients with advanced ***,we investigated the effect of EAF2 on the invasion,migration,and angiogenesis of CRC cells in vitro using plasmid *** EAF2 protein was lowly expressed in cancer tissues of patients with advanced ***-Meier survival analysis showed that the survival rate of the high EAF2 level group was higher than that of the low EAF2 level *** Our results demonstrated that EAF2,as a tumor suppressor,may inhibit the invasion,metastasis,and angiogenesis of CRC cells by regulating the signal transducer and activator of transcription 3/transforming growth factor-β1 crosstalk pathway,and play a cancer suppressive and protective role in the occurrence and development of *** findings are of great significance to provide a new idea and theoretical basis for the targeted diagnosis and treatment of CRC.

关键词： ELL-associated factor 2 Transforming growth factor-β1 Signal transducer and activator of transcription 3 Colorectal cancer Invasion Migration Angiogenesis

Role and mechanism of angiotensin-converting enzyme 2 in acute lung injury in coronavirus disease 2019

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Chronic Diseases and Translational Medicine 2020年第2期6卷 98-105页

作者： meng-yuan liu Bo Zheng Yan Zhang Jian-Ping Li Department of Cardiology Peking University First HospitalBeijing 100034China NHC Key Laboratory'of Cardiovascular Molecular Biology and Regulatory Peptides Beijing 100034China Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education Health Science CenterPeking UniversityBeijing 100034China

Coronavirus disease 2019 is a major threat to public health *** its pathogenesis has not been fully elucidated,angiotensin-converting enzyme 2(ACE2)has been recently identified as a receptor for the entry of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)into the ***,we aimed to clarify the potential role of ACE2 in SARS-CoV-2-induced acute lung injury and its underlying *** a receptor for coronavirus,ACE2 mediates the entry of SARS-CoV-2 into cells in a similar way as for severe acute respiratory syndrome coronavirus(SARS-CoV).The high binding affinity of SARS-CoV-2 to ACE2 correlates with its efficient spread among *** the other hand,ACE2 negatively regulates the renin-angiotensinaldosterone system(RAAS)primarily by converting angiotensinⅡto angiotensin *** exerts a beneficial effect on coronavirus-induced acute lung *** recombinant ACE2 has been considered as a potential therapy for SARS-CoV-2 by blocking virus entry and redressing the imbalance of RAAS in SARS-CoV-2 *** level of ACE2 expression can be upregulated by treatment with an ACE inhibitor(ACEI)or angiotensinⅡtype 1 receptor blocker(ARB).To date,no evidence shows that ACEIs or ARBs increase the susceptibility and mortality of patients infected with SARS-CoV-2,and hence,it is not advisable to discontinue such drugs in patients with cardiovascular disease.

关键词： Severe acute respiratory syndrome coronavirus 2 Angiotensin-converting enzyme 2 Acute lung injury

Modulated noble metal/2D MOF heterostructures for improved hydrogen storage of MgH_(2)

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Rare Metals 2024年第4期43卷 1672-1685页

作者： Zeng-Yi Li Li-Xian Sun Fen Xu Yu-Mei Luo Yong-Peng Xia Sheng Wei Chen-Chen Zhang Ri-Guang Cheng Chao-Feng Ye meng-yuan liu Ju-Lan Zeng Zhong Cao Hong-Ge Pan School of Material Science and Engineering Guilin University of Electronic TechnologyGuilin541004China School of Chemistry and Chemical Engineering Changsha University of Science and TechnologyChangsha410114China Institute of Science and Technology for New Energy Xi’an Technological UniversityXi’an710021China

The conjugation of external species with twodimensional(2D)materials has broad application *** this study,we have explored the potential of noble metal/2D MOF heterostructures in hydrogen ***,the MgH_(2)-Ni-MOF@Pd system has shown remarkable hydrogen desorption/sorption performances,starting to liberate hydrogen at 1810C,which is 2300C lower than that of pristine *** the catalytic effect of Ni-MOF@Pd,the dehydrogenation apparent activation energy of MgH2 is noticeably decreased from(133.5±17.5)to(34.58±1.87)kJ·moL^(-1),and the hydrogenation apparent activation energy is reduced from(70.41±7.43)to(25.78±4.64)kJ·moL^(-1),which is lowered by 63.4%.The fully-dehydrogenated MgH2-NiMOF@Pd composite rapidly uptakes hydrogen,with 2.62wt%at 100℃and 6.06 wt%at 150℃within 300 s,*** mechanism analysis of MgH2 catalyzed by Ni-MOF@Pd has revealed that the transformation of Mg_2Ni and Mg_2NiH_4 could act as a"hydrogen pump",providing numerous channels for fast diffusion and transport of hydrogen ***,in the dehydrogenation process,the element Pd reacts with MgH_(2)to form the MgPd alloy phase,which makes MgH_(2)take precedence to decompose through the Mg-Pd alloy rather than self-decomposition,further reducing thermal stability and improving de/hydrogenation *** synergistic effect of Mg-Pd,Mg_2Ni,and the special ultra-thin 2D sheet structure of the additive is the main reason for the good hydrogen storage property of MgH_(2)-Ni-MOF@*** findings provide inspiration for designing efficient multifunctional additives with unique morphologies to optimize the hydrogen desorption/sorption behaviors of hydrogen storage materials.

关键词： Hydrogen storage Magnesium hydride 2D metal-organic framework nanosheets Ni/Pd Synergistic catalysis

Novel and Effective Antioxidant of Polyphenol Oxidase Designing by Combing Molecular Simulation, Optimized Synthesis and Biological Verification

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Novel and Effective Antioxidant of Polyphenol Oxidase Design...

泵浦激光功率和气室温度对SERF陀螺仪的影响（英文）

中国化学会第一届农业化学学术讨论会

作者： Xiao-Juan Qi meng-yuan liu Qing-Ye Zhang Hubei Key Laboratory of Agricultural Bioinformatics College of Informatics Huazhong Agricultural University

The enzymatic reaction of polyphenol oxidase（PPO） is the main reason leading to browning of lotus root, which has caused huge economic losses. It is urgent to develop novel and efficient PPO inhibitors. In this study, comprehensive using virtual screening and lead compound optimization, 21 novel N-substituted phenyl-hydrazides thiosemicarbazide derivatives were produced by one-pot synthetic method. All the structures of the target compounds were characterized by IR, and 1 H NMR spectrum. The bioactive assay results showed that 19 of the derivatives displayed better inhibition ability to PPO than the commercialized PPO inhibitor, ascorbic acid. To quantify the relationship between structure and the activity of the target compounds, a CoMFA model with high predict abilities（q2 = 0.607, r2 = 0.997, N = 2, SEE = 0.045, F = 304.588） was built. The model revealed that the differences in the biological activity between the derivatives were due to two aspects, bulky groups in R1 substitute and polar atoms in R2 substitute. Finally, according to the enzyme activity and color trait of target compounds, 9, 15, 16, 17, A2 and A8 compounds were screened out to perform overall quality, L value and polyphenol oxidase activity measured at four-day interval, the results showed that compounds A2 and A8 displayed more effective active than the commercialized PPO inhibitor in inhibiting the browning on the overall appearance quality of fresh-cut lotus root slices and increasing storage time in some degree.

关键词： Polyphenol oxidase Antioxidant Virtual screening 3D-QSAR Biological testing

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中国惯性技术学报 2017年第6期25卷 810-814,820页

作者：石猛刘院省王学锋王巍北京航天控制仪器研究所北京100854

SERF陀螺仪工作在无自旋交换弛豫(SERF)状态下,电子自旋和核子自旋强烈耦合。通过研究电子自旋与核子自旋的耦合方程,分析了泵浦激光的功率和气室温度对SERF陀螺仪磁场补偿的影响,并搭建了SERF陀螺仪实验平台系统进行验证。研究结果表明... 详细信息

SERF陀螺仪工作在无自旋交换弛豫(SERF)状态下,电子自旋和核子自旋强烈耦合。通过研究电子自旋与核子自旋的耦合方程,分析了泵浦激光的功率和气室温度对SERF陀螺仪磁场补偿的影响,并搭建了SERF陀螺仪实验平台系统进行验证。研究结果表明:原子核自旋补偿磁场正比于泵浦激光功率,泵浦激光功率在9.7 m W至105.6 m W的变化范围中,自旋补偿磁场的变化达到8.9 n T;气室温度在107.5℃至149.3℃变化范围中,自旋补偿磁场变化为4.5 n T,无明显的相关性,并且相对于气室温度的变化,补偿磁场对泵浦激光功率变化更加敏感。

关键词： SERF陀螺仪原子自旋陀螺仪无自旋交换弛豫自旋耦合

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国内外口腔疾病负担的现况和分析

中华口腔医学杂志 2017年第6期52卷 386-389页

作者：孟圆刘雪楠郑树国北京大学口腔医学院·口腔医院预防科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室 100081

口腔疾病目前已成为世界上大部分人群的健康问题之一。口腔疾病负担研究是对口腔疾病造成的社会经济和人群健康压力与影响的研究，是国内外各个国家地区需要重视的公共卫生问题。本文通过文献回顾的方法，总结了口腔疾病负担在国内外的... 详细信息

口腔疾病目前已成为世界上大部分人群的健康问题之一。口腔疾病负担研究是对口腔疾病造成的社会经济和人群健康压力与影响的研究，是国内外各个国家地区需要重视的公共卫生问题。本文通过文献回顾的方法，总结了口腔疾病负担在国内外的研究进展，通过患病率、龋均、伤残调整寿命年、伤残权重等指标对口腔疾病的流行病学负担进行比较，并通过一些数据说明口腔疾病的经济负担现状。结果表明口腔疾病在国内外大部分国家、地区均为明显的疾病负担，而我国对口腔疾病负担的研究尚不成熟，以龋病、牙周病、失牙为主的口腔疾病在我国患病率高，社会大众的就诊率低，且医疗费用自付比例高，相对而言，我国口腔疾病的流行病学和经济负担较重。

关键词：患病代价口腔疾病负担流行病学负担经济负担