Phosphorylation at Ser28 stabilizes the Arabidopsis nitrate transporter NRT2.1 in response to nitrate limitation

作     者：Xue Zou Meng-Yuan Liu Wei-Hua Wu Yang Wang Xue Zou;Meng-Yuan Liu;Wei-Hua Wu;Yang Wang

作者机构：State Key Laboratory of Plant Physiology and BiochemistryCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2020年第62卷第6期

页      面：865-876页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：the National Natural Science Foundation of China(31400222) 

主　　题：nitrate Arabidopsis stabilization 

摘      要：Nitrate is one of the main inorganic nitrogen sources for *** absorption from soils is achieved through the combined activities of specific nitrate *** transporter 2.1(NRT2.1)is the major component of the root high-affinity nitrate transport system in Arabidopsis *** to date have mainly focused on transcriptional control of ***,we show that NRT2.1 protein stability is also regulated in response to nitrogen nutrition *** seedlings were transferred to nitrate-limited conditions,the apparent half-life of NRT2.1 in roots increased from 3 to 9 *** stabilization of NRT2.1 protein occurred rapidly,even prior to the transcriptional stimulation of ***,we revealed that phosphorylation at serine28(Ser28)of NRT2.1 is involved in regulating the stability of this *** of Ser28 by alanine resulted in unstable NRT2.1,and this loss-of-phosphorylation mutant(NRT2.1S28A)failed to complement the growth-restricted phenotype of the nrt2.1 mutant when a low concentration of nitrate was the sole nitrogen *** results demonstrate that phosphorylation at Ser28 is crucial for NRT2.1 protein stabilization and accumulation in response to nitrate limitation.