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Spatio-temporal dynamics of phytohormones in the tomato graft healing process

Horticultural Plant Journal 2024年第6期10卷 1362-1370页

作者： Duan Y. Zhang F. meng x. Shang Q. State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China

Graft healing involves a series of cytological and molecular events including wound responses, callus formation and vascular bundle remodelling. Hormones are important signalling molecules regulating plant development and responses to environmental stimuli. However, the detailed dynamics of phytohormones in graft healing remain elusive. In this research, internodes above and below the graft site were harvested from 0 to 168 h after grafting (HAG), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to determinate jasmonic acid, aux.n, cytokinin, ethylene, salicylic acid, abscisic acid and gibberellin levels during the graft healing process. Uniform manifold approx.mation and projection (UMAP) and k-means analyses were performed to ex.lore hormone spatio-temporal dynamics. We found the stage-specific and asymmetric accumulation of phytohormones in the tomato graft healing process. At the early healing stage (before vascular bundle reconnection), IAA, cZ, ABA, JA and SA mainly accumulated above the graft site, while tZ and ACC mainly accumulated below the graft site. MEIAA, ICAld and IP mainly accumulated at the later stage. Comminated with the healing process, we suggested that JA is mainly involved in wound responses, IAA is beneficial to the formation of callus and vascular cell development, tZ promotes cell division, and IP is linked to vascular bundle remodelling. In addition, ex.ression of JA-related genes SlMYC2 and SlJAZ2, IAA-related gene SlIAA1, tZ-related genes SlHP2 and SlRR8, and IP-related gene SlRR9 correlated with hormone accumulation. The findings provide important information about the hormones and genes involved in the tomato graft healing process. © 2023 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

关键词： Gene Graft healing Phytohormone Tomato Uniform manifold approx.mation and projection

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Genome-wide analyses of miRNAs in mycorrhizal plants in response to late blight and elucidation of the role of miR319c in tomato resistance

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Horticultural Plant Journal 2024年第6期10卷 1371-1382页

作者： Zhou x. Wang Z. Su C. Cui J. meng J. Luan Y. School of Bioengineering Dalian University of Technology Liaoning Dalian 116024 China College of Life Science Hunan Normal University Hunan Changsha 410081 China School of Computer Science and Technology Dalian University of Technology Liaoning Dalian 116024 China

Tomato (Solanum lycopersicum), an economically important vegetable crop cultivated worldwide, often suffers massive financial losses due to Phytophthora infestans (P. infestans) spread and breakouts. Arbuscular mycorrhiza (AM) fungi mediated biocontrol has demonstrated great potential in plant resistance. However, little information is available on the regulation of mycorrhizal tomato resistance against P. infestans. Therefore, microRNAs (miRNAs) sequencing technology was used to analyse miRNA and their targets in the mycorrhizal tomato after P. infestans infection. Our study showed a lower severity of necrotic lesions in mycorrhizal tomato than in nonmycorrhizal controls. We investigated 35 miRNAs that showed the opposite ex.ression tendency in mycorrhizal and nonmycorrhizal tomato after P. infestans infection when compared with uninfected P. infestans. Among them, miR319c was upregulated in mycorrhizal tomato leaves after pathogen infection. Overex.ression of miR319c or silencing of its target gene (TCP1) increased tomato resistance to P. infestans, implying that miR319c acts as a positive regulator in tomato after pathogen infection. Additionally, we ex.mined the induced ex.ression patterns of miR319c and TCP1 in tomato plants ex.osed to salicylic acid (SA) treatment, and SA content and the ex.ression levels of SA-related genes were also measured in overex.ressing transgenic plants. The result revealed that miR319c can not only participates in tomato resistance to P. infestans by regulating SA content, but also indirectly regulates the ex.ression levels of key genes in the SA pathway by regulating TCP1. In this study, we propose a novel mechanism in which the miR319c in mycorrhizal tomato increases resistance to P. infestans. © 2023 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

关键词： Arbuscular mycorrhiza fungi miRNA Phytophthora infestans Tomato

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Accelerating bainite transformation by concurrent pearlite formation in a medium Mn steel: Ex.eriments and modelling

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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY 2024年第000期176卷 211-223页

作者： Huang, L. K. Liu, F. Huang, M. x. Univ Hong Kong Dept Mech Engn Pokfulam Rd Hong Kong Peoples R China Northwestern Polytech Univ State Key Lab Solidificat Proc Xian 710072 Shaanxi Peoples R China

Bainite transformation has yet to be utilized and even thoroughly studied in medium Mn steels. Here, we investigate the isothermal bainite transformation in a 10Mn steel at 450 degrees C ex.erimentally and theoret-ically, focusing on the effect of dislocations introduced by warm deformation. We show that the bainite transformation in the studied medium Mn steel ex.ibits ex.remely sluggish kinetics (on a time scale of days), concurrent with the pearlite formation. The introduced dislocations can significantly accelerate bai-nite transformation kinetics while also facilitating the pearlite reaction. This is likely the first report on the simultaneous occurrence of these two solid-state reactions in medium Mn steels. With respect to the roles of dislocations in the acceleration of bainite transformation observed in this work, we propose a new 'carbon depletion mechanism', in which dislocations-stimulated pearlite formation makes a twofold contribution: facilitating the formation of bainitic ferrite sub-units to further enhance the autocatalytic effect and preventing the carbon enrichment in the remaining austenite. On this basis, a physical model is developed to quantitatively understand the bainite transformation kinetics considering the effect of concurrent pearlite formation, revealing good agreements between model descriptions and ex.eriment results. Our findings, herein, offer fundamental insights into the bainite transformation in medium Mn steels and uncover a previously unidentified role played by introduced dislocations in influencing the ki-netics of bainite formation, which may guide its future application in manipulating microstructure for the development of advanced high-strength steels. (c) 2023 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.

关键词： Medium Mn steels Bainite Thermodynamics Kinetics Phase transformation AUSTENITE GRAIN-SIZE LOW-ALLOY STEELS ATOM-PROBE FE-C MECHANICAL-PROPERTIES QUANTITATIVE-ANALYSIS SOLUTE DRAG KINETICS CARBON FERRITE

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The landscape and clinical relevance of intronic polyadenylation in human cancers

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Journal of Genetics and Genomics 2024年第10期51卷 1030-1039页

作者： Cheng x. Jiang G. Zhou x. Wang J. Zhao Z. Zhang J. Ni T. State Key Laboratory of Genetic Engineering National Clinical Research Center for Aging and Medicine Huashan Hospital Collaborative Innovation Center of Genetics and Development Human Phenome Institute Center for Evolutionary Biology Shanghai Engineering Research Center of Industrial Microorganisms School of Life Sciences Fudan University Shanghai 200438 China MOE Key Laboratory of Contemporary Anthropology School of Life Sciences Fudan University Shanghai 200438 China State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock Institutes of Biomedical Sciences School of Life Sciences Inner Mongolia University Inner Mongolia Hohhot 010070 China

Intronic polyadenylation (IPA) is an RNA 3′ end processing event which has been reported to play important roles in cancer development. However, the comprehensive landscape of IPA events across various cancer types is lacking. Here, we apply IPAFinder to identify and quantify IPA events in 10,383 samples covering all 33 cancer types from The Cancer Genome Atlas (TCGA) project. We identify a total of 21,835 IPA events, almost half of which are ubiquitously ex.ressed. We identify 2761 unique dynamically changed IPA events across cancer types. Furthermore, we observe 8855 non-redundant clinically relevant IPA events, which could potentially be used as prognostic indicators. Our analysis also reveals that dynamic IPA usage within cancer signaling pathways may affect drug response. Finally, we develop a user-friendly data portal, IPACancer Atlas (http://***/Pancan_IPA/), to search and ex.lore IPAs in cancer. © 2024

关键词： Cancer Database Drug sensitivity Intronic polyadenylation Single nucleotide variant

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Isolation of Trichoderma virens 6PS-2 and its effects on Fusarium proliferatum f. sp. Malus domestica MR5 related to apple replant disease in China

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Horticultural Plant Journal 2024年第6期10卷 1291-1308页

作者： Wang H. Tang W. Mao Y. Ma S. Chen x. Shen x. Yin C. Mao Z. State Key Laboratory of Crop Biology College of Horticultural Science and Engineering Shandong Agricultural University Shandong Tai'an 271018 China

Apple replant disease (ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens (such as Fusarium spp.) that cause ARD is of great significance to the sustainable development of the apple industry. Trichoderma virens 6PS-2, which ex.ibited antagonism toward a variety of pathogens, was screened from the rhizosphere soils of healthy apple trees (Malus robusta) in different replanted orchards in the Yantai and Zibo Cities, Shandong Province, China. Its fermentation ex.ract inhibited the growth of pathogenic Fusarium proliferatum f. sp. Malus domestica MR5, which was proportional to the concentration. These substances also increased the hairy root volume and growth of Arabidopsis thaliana lateral roots. The phenotype of Malus hupehensis seedlings and microbial community structure in rhizosphere soils in greenhouse ex.eriment using High-throughput sequencing were analyzed, and the field ex.eriment with grafted apple trees were used for further verification. Compared with the application of potato dex.rose broth (PDB) medium, application of 6PS-2 spore suspension directly to replanted soils could improve the growth of M. hupehensis seedlings as well as the elongation of grafted apple trees. Concomitant decreases in the gene copy number of Fusarium and increases in the culturable bacteria/fungi were also observed in the greenhouse and field ex.eriments. The abundance of Trichoderma, Bacillus, and Streptomyces increased significantly, but that of Fusarium, Pseudarthrobacter, and Humicola decreased. The content of esters, phenols, furans, and amino acids in root ex.dates of M. hupehensis seedlings increased, which significantly inhibited the multiplication of Fusarium, but was positively correlated with Bacillus and Trichoderma. In summary, T. virens 6PS-2 not only directly inhibits the activity of pathogenic Fusarium but also secrets secondary metabol

关键词： Apple replant disease Biocontrol High-throughput sequencing Secondary metabolites Spore suspension Trichoderma virens 6PS-2

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A positive feedback loop between PLD1 and NF-κB signaling promotes tumorigenesis of nasopharyngeal carcinoma

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Journal of Genetics and Genomics 2024年第10期51卷 997-1006页

作者： Zhou Y.-Q. Cheng x.-x. He S. Liu S.-Q. Li Y.-Q. Wei P.-P. Luo C.-L. Bei J.-x. State Key Laboratory of Oncology in South China Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy Guangdong Provincial Clinical Research Center for Cancer Collaborative Innovation Center for Cancer Medicine Sun Yat-sen University Cancer Center Guangzhou 510060 China Department of Ex.erimental Research Sun Yat-sen University Cancer Center Guangzhou 510060 China Department of Medical Oncology National Cancer Centre of Singapore Singapore

Phospholipase D (PLD) lipid-signaling enzyme superfamily has been widely implicated in various human malignancies, but its role and underlying mechanism remain unclear in nasopharyngeal carcinoma (NPC). Here, we analyze the ex.ressions of 6 PLD family members between 87 NPC and 10 control samples through transcriptome analysis. Our findings reveal a notable upregulation of PLD1 in both NPC tumors and cell lines, correlating with worse disease-free and overall survival in NPC patients. Functional assays further elucidate the oncogenic role of PLD1, demonstrating its pivotal promotion of critical tumorigenic processes such as cell proliferation and migration in vitro, as well as tumor growth in vivo. Notably, our study uncovers a positive feedback loop between PLD1 and the NF-κB signaling pathway to render NPC progression. Specifically, PLD1 enhances NF-κB activity by facilitating the phosphorylation and nuclear translocation of RELA, which in turn binds to the promoter of PLD1, augmenting its ex.ression. Moreover, RELA overex.ression markedly rescues the inhibitory effects in PLD1-depleted NPC cells. Importantly, the application of the PLD1 inhibitor, VU0155069, substantially inhibits NPC tumorigenesis in a patient-derived x.nograft model. Together, our findings identify PLD1/NF-κB signaling as a positive feedback loop with promising therapeutic and prognostic potential in NPC. © 2024 The Authors

关键词： Nasopharyngeal carcinoma (NPC) NF-κB pathway PLD1 Positive feedback loop RELA Tumorigenesis

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Polydopamine wrapped polyaniline nanosheets: Synthesis and anticorrosion application for waterborne epox. coatings

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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY 2024年第000期176卷 155-166页

作者： Hu, x. P. Zhang, Y. H. Liu, C. B. Cui, H. Z. Shandong Univ Sci & Technol Sch Mat Sci & Engn Qingdao 266590 Peoples R China Ocean Univ China Sch Mat Sci & Engn Qingdao 266100 Peoples R China

Two-dimensional materials have been proved to be effective in improving the performance of anticorrosive coatings. However, most of the reported two-dimensional materials only have the ability to passively block corrosive media and cannot effectively inhibit localized corrosion reactions at the metal/coating interface. Herein, we first synthesized a novel polyaniline (PANI) nanosheet with both barrier and passivation functions for metals. In addition, polydopamine (PDA) wrapped PANI nanosheets (PANI@PDA) were obtained by in situ self-polymerization reaction of dopamine on the PANI surface, which enhance its interfacial interaction with polymeric resin. The chemical structure, morphology and corrosion inhibition properties of the nanosheets were systematically analyzed. The incorporated PANI@PDA nanosheets in composite coating provide a longer penetration path for corrosive mediums. Secondly, the uniformly dispersed two-dimensional nanosheets induce the formation of passivation film on the metal substrate. The protective effect of PANI@PDA nanosheets on coatings was verified by electrochemical impedance spectroscopy (EIS). Results indicated that the prepared composite coating ex.ibited ex.ellent corrosion resistance throughout the test cycle. This simple and effective modification strategy based on conductive polymers provides a new insight into the development of environmentally adaptive composite coatings with active and passive protection.(c) 2023 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.

关键词： Corrosion prevention Polyaniline Polydopamine Nanosheet Active protection CORROSION PROTECTION SURFACE-CHEMISTRY GRAPHENE STEEL PERFORMANCES COMPOSITES

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Species-specific and generalized allometric biomass models for eight Fagaceae species in the understory of evergreen broadleaved forests in subtropical China

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Journal of Forestry Research 2024年第4期35卷 156-170页

作者： Shengwang meng Qianyanzhou Ecological Research Station Key Laboratory of Ecosystem Network Observation and ModelingInstitute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101People’s Republic of China

Quantifying the biomass of saplings in the regeneration component is critical for understanding biogeochemical processes of forest ***,accurate allometric equations have yet to be developed in sufficient *** develop species-specific and generalized allometric equations,154 saplings of eight Fagaceae tree species in subtropical China’s evergreen broadleaved forests were *** dendrometric variables,root collar diameter(d),height(h),and crown area(ca)were applied in the model by the weighted nonlinear seemingly unrelated regression *** only d as an input variable,the species-specific and generalized allometric equations estimated the aboveground biomass reasonably,with R _(adj)^(2) values generally>*** h and/or ca improved the fitting of some biomass components to a certain *** equations showed a relatively large coefficient of variation but comparable bias to species-specific *** in the absence of species-specific equations at a given location are generalized equations for mix.d species *** developed regression equations can be used to accurately calculate the aboveground biomass of understory Fagaceae regeneration trees in China’s subtropical evergreen broadleaved forests.

关键词： Allometry Aboveground biomass Additivity Regeneration Subtropical China

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Genome-editing of a circadian clock gene TaPRR95 facilitates wheat peduncle growth and heading date

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Journal of Genetics and Genomics 2024年第10期51卷 1101-1110页

作者： Fu M. Liu S. Che Y. Cui D. Deng Z. Li Y. Zou x. Kong x. Chen G. Zhang M. Liu Y. Wang x. Liu W. Liu D. Geng S. Li A. Mao L. State Key Laboratory of Crop Gene Resources and Breeding and National Key Facility for Crop Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China College of Life Sciences Henan Normal University Henan Xinxiang 453007 China College of Agronomy Sichuan Agricultural University Sichuan Chengdu 610106 China College of Agronomy Henan Agricultural University Henan Zhengzhou 450002 China School of Life Science Shanxi University Shanxi Taiyuan 030006 China

Plant height and heading date are important agronomic traits in wheat (Triticum aestivum L.) that affect final grain yield. In wheat, knowledge of pseudo-response regulator (PRR) genes on agronomic traits is limited. Here, we identify a wheat TaPRR95 gene by genome-wide association studies to be associated with plant height. Triple allele mutant plants produced by CRISPR/Cas9 show increased plant height, particularly the peduncle, with an earlier heading date. The longer peduncle is mainly caused by the increased cell elongation at its upper section, whilst the early heading date is accompanied by elevated ex.ression of flowering genes, such as TaFT and TaCO1. A peduncle-specific transcriptome analysis reveals up-regulated photosynthesis genes and down-regulated IAA/Aux.genes for aux.n signaling in prr95aabbdd plants that may act as a regulatory mechanism to promote robust plant growth. A haplotype analysis identifies a TaPRR95-B haplotype (Hap2) to be closely associated with reduced plant height and increased thousand-grain weight. Moreover, the Hap2 frequency is higher in cultivars than that in landraces, suggesting the artificial selection on the allele during wheat breeding. These findings suggest that TaPRR95 is a regulator for plant height and heading date, thereby providing an important target for wheat yield improvement. © 2024 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China

关键词： CRISPR/Cas9 Heading date Photosynthesis Phytohormone Plant height Pseudo-response regulator (PRR) TaPRR95 Wheat

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Additional far-red light promotes adventitious rooting of double-root-cutting grafted watermelon seedlings

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Horticultural Plant Journal 2024年第6期10卷 1424-1436页

作者： Wu x. Zhang x. Wang Y. Wu C. Sun Y. Zhang Y. Ji Y. Bao E. x.a L. Bian Z. Cao K. The Agriculture Ministry Key Laboratory of Agricultural Engineering in the Middle and Lower Reaches of Yangtze River Institute of Agricultural Facilities and Equipment Jiangsu Academy of Agricultural Sciences Jiangsu Nanjing 210014 China Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China College of Horticulture Shanxi Agricultural University Shanxi Taigu 030801 China Huaiyin Institute of Agricultural Science in x.huai District Jiangsu Huaian 223001 China Horticulture and Product Physiology Department of Plant Sciences Wageningen University & Research Wageningen 6700AA Netherlands Institute of Urban Agriculture Chinese Academy of Agricultural Sciences Sichuan Chengdu 610299 China

Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting. To date, little information is available on how to enhance root regeneration of rootstock in grafted watermelon (Citrullus lanatus) seedlings. In this study, the effects of different light treatments on root regeneration were determined. This revealed that addition of far-red light (Fr) could significantly ex.edite root formation in the rootstock. Moreover, the results of transcriptome analysis revealed that plant hormone pathway and aux.n-related genes were greatly induced by Fr, especially for aux.n-response proteins (including CmIAA11, CmIAA17, and CmAUx.8), Small aux.n-up RNA genes (including CmSAUR20 and CmSAUR50) and the aux.n efflux.transporter (CmPIN3). In addition, the ex.ression of Phytochrome Interacting Factor (PIFs), such as CmPIF1, CmPIF3 and CmPIF7, was remarkably increased by Fr. These genes may act together to activate aux.n-related pathways under Fr treatment. Based on the results of HPLC-MS/MS analysis, the concentrations of different aux.n-types in adventitious root were significantly influenced by Fr. Furthermore, the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment, which was determined by the acid magenta dyeing ex.eriment. Therefore, all the results suggested that Fr could induce AR formation in rootstocks, which may be associated with the aux.n accumulation by regulating the transcriptional level of aux.n-related and PIF genes. The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings, which will provide a theoretical basis for the speeding development of industrialized seedlings production. © 2024 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

关键词： Adventitious root regeneration Aux.n Double-root-cutting grafting Rootstock Watermelon

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