Spatio-temporal dynamics of phytohormones in the tomato graft healing process
作者机构：State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China
出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)
年 卷 期：2024年第10卷第6期
页 面：1362-1370页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：Chinese Academy of Agricultural Sciences, CAAS National Key Research and Development Program of China, NKRDPC, (2020YFD1000300) National Key Research and Development Program of China, NKRDPC earmarked fund for CARS, (CARS-23-B10) Key Research and Development Project of Hainan Province, (ZDKJ2021005) Key Research and Development Project of Hainan Province Key Technology Research and Development Program of Shandong Province, (LJNY202106) Key Technology Research and Development Program of Shandong Province
主 题：Gene Graft healing Phytohormone Tomato Uniform manifold approximation and projection
摘 要：Graft healing involves a series of cytological and molecular events including wound responses, callus formation and vascular bundle remodelling. Hormones are important signalling molecules regulating plant development and responses to environmental stimuli. However, the detailed dynamics of phytohormones in graft healing remain elusive. In this research, internodes above and below the graft site were harvested from 0 to 168 h after grafting (HAG), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to determinate jasmonic acid, auxin, cytokinin, ethylene, salicylic acid, abscisic acid and gibberellin levels during the graft healing process. Uniform manifold approximation and projection (UMAP) and k-means analyses were performed to explore hormone spatio-temporal dynamics. We found the stage-specific and asymmetric accumulation of phytohormones in the tomato graft healing process. At the early healing stage (before vascular bundle reconnection), IAA, cZ, ABA, JA and SA mainly accumulated above the graft site, while tZ and ACC mainly accumulated below the graft site. MEIAA, ICAld and IP mainly accumulated at the later stage. Comminated with the healing process, we suggested that JA is mainly involved in wound responses, IAA is beneficial to the formation of callus and vascular cell development, tZ promotes cell division, and IP is linked to vascular bundle remodelling. In addition, expression of JA-related genes SlMYC2 and SlJAZ2, IAA-related gene SlIAA1, tZ-related genes SlHP2 and SlRR8, and IP-related gene SlRR9 correlated with hormone accumulation. The findings provide important information about the hormones and genes involved in the tomato graft healing process. © 2023 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).
维普期刊数据库