Genome-wide analyses of miRNAs in mycorrhizal plants in response to late blight and elucidation of the role of miR319c in tomato resistance
作者机构：School of Bioengineering Dalian University of Technology Liaoning Dalian 116024 China College of Life Science Hunan Normal University Hunan Changsha 410081 China School of Computer Science and Technology Dalian University of Technology Liaoning Dalian 116024 China
出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)
年 卷 期：2024年第10卷第6期
页 面：1371-1382页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学]
基 金：National Natural Science Foundation of China, NSFC, (32230091, 32072592) National Natural Science Foundation of China, NSFC
主 题：Arbuscular mycorrhiza fungi miRNA Phytophthora infestans Tomato
摘 要：Tomato (Solanum lycopersicum), an economically important vegetable crop cultivated worldwide, often suffers massive financial losses due to Phytophthora infestans (P. infestans) spread and breakouts. Arbuscular mycorrhiza (AM) fungi mediated biocontrol has demonstrated great potential in plant resistance. However, little information is available on the regulation of mycorrhizal tomato resistance against P. infestans. Therefore, microRNAs (miRNAs) sequencing technology was used to analyse miRNA and their targets in the mycorrhizal tomato after P. infestans infection. Our study showed a lower severity of necrotic lesions in mycorrhizal tomato than in nonmycorrhizal controls. We investigated 35 miRNAs that showed the opposite expression tendency in mycorrhizal and nonmycorrhizal tomato after P. infestans infection when compared with uninfected P. infestans. Among them, miR319c was upregulated in mycorrhizal tomato leaves after pathogen infection. Overexpression of miR319c or silencing of its target gene (TCP1) increased tomato resistance to P. infestans, implying that miR319c acts as a positive regulator in tomato after pathogen infection. Additionally, we examined the induced expression patterns of miR319c and TCP1 in tomato plants exposed to salicylic acid (SA) treatment, and SA content and the expression levels of SA-related genes were also measured in overexpressing transgenic plants. The result revealed that miR319c can not only participates in tomato resistance to P. infestans by regulating SA content, but also indirectly regulates the expression levels of key genes in the SA pathway by regulating TCP1. In this study, we propose a novel mechanism in which the miR319c in mycorrhizal tomato increases resistance to P. infestans. © 2023 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).
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