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Immune response to inactivated bacterial vector carrying the recombinant K39 antigen of Leishmania infantum in mice

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Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 199-206页

作者： Lucelina S.Araújo Bruno B.Silva Eduarda N.F.N.Santos Arnaldo S.Bezerra Samuel S.Frota Assis R.Montenegro Eridan O.P.T.Florean maur.cio Fvan Tilburg Maria Izabel F.Guedes Laboratory of Biotechnology and Molecular Biology Northeast Biotechnology NetworkState University of CearáFortalezaBrazil Faculty of Veterinary Medicine State University of CearáFortalezaBrazil

Objective:To evaluate the immunological response elicited by an inactivated bacterial vector carrying the K39 antigen of Leishmania infantum,and a purified ***:Mice were subjected to the following treatments:(1)Purified recombinant K39(rK39)protein at a 20μg dose with complete Freund’s adjuvant;(2)Inactivated Escherichia coli(BL21 DE3)carrying the K39 protein at an equivalent total protein content of 200μg;(3)Inactivated bacteria lacking the K39 protein;(4)Non-immunized control *** monitoring was *** groups were challenged by intraperitoneal injection of 10^(7) Leishmania infantum *** euthanasia,the liver and spleen were collected to analyze the levels of TNF,IFN-γ,IL-12,IL-4,and ***:Mice immunized with purified rK39 or the inactivated bacterial vector carrying the K39 antigen of Leishmania infantum showed a long-lasting immune response with high levels of polyclonal antibodies specifically recognizing the recombinant *** IgG1 subclass was the predominant immunoglobulin;however,the induction of IgG2a and the profile of cytokines produced were indicative of the induction of a mixed-type ***:The inactivated bacterial vector carrying the K39 antigen,as well as the purified antigen can induce a long-lasting immune response in immunized mice,predominantly favouring a Th2 profile response.

关键词： Visceral leishmaniasis K39 Inactivated bacterial vector Vaccine Immune response Th1 Th2 Leishmania infantum

In-Hospital Outcomes in Minimally Invasive Mitral Valve Surgery: First Results in a Brazilian Single Center

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Open Journal of Thoracic Surgery 2024年第1期14卷 17-28页

作者： Daniel de Magalhães Freitas João Alberto Pansani Max Weyler Nery Stanlley de Oliveira Loyola maur.cio Lopes Prudente Giulliano Gardenghi Artur Henrique de Souza Department of Cardiovascular Surgery Encore Hospital Aparecida de Goiania Brazil Anesthesia Clinic (Clianest) Goiania Brazil

Introduction: Treatments for cardiovascular diseases have increasingly evolved with the tendency to offer minimally invasive or transcatheter procedures instead of conventional sternotomy surgery. In this context, we highlight minimally invasive mitral valve surgery (MIMVS), which has been shown to be an increasingly solid option with some superior results when compared to the conventional technique: better pain control, shorter hospital stays, shorter recovery time, shorter readmission rate in the first postoperative year, better aesthetic results, and lower overall cost. Aim: This study aims to evaluate the stages of MIMVS, by primary mitral valve consultation, in our service and compare these results with data from the literature. Methods: All electronic medical records of patients who underwent MIMVS for primary mitral valve injury in the Encore Hospital from January 2020 to February 2023 were analyzed. Tabulation and statistical analysis were performed using the Microsoft Excel® program. Quantitative variables were presented as means, standard deviations. Results: 46 patients were enrolled in our study (Age: 59.1 ± 12.4 years old;60.8% Female, BMI: 26 ± 4.4 Kg/m2, Low risk STS score: 82.6%). The observed 30-day mortality was 2.1%, plastic rate of 23.9%, blood transfusion rate of 41.3%, length of stay in an intensive care bed (ICB) of 3.3 ± 3.3 days and hospital stay of 6.4 ± 5.1 days. Conclusions: We noticed that the MIMVS results carried out in our service agree with data from national and international literature with approximately 1.3 days more hospitalization in ICB.

关键词： Minimally Invasive Surgical Procedures Mitral Valve Outcome Assessment Health Care

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波导海面杂波频谱结构的模式

电波与天线 1990年第5期 80-88,100页

作者： maur.,DE 黄瑞东

Genetic diversity and structure of Drimys brasiliensis in southern Brazil:insights for conservation

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Journal of Forestry Research 2020年第4期31卷 1325-1332页

作者： Alexandre Mariot Tiago Montagna maur.cio Sedrez dos Reis Núcleo de Pesquisas Em Florestas Tropicais Pós-Graduacāo Em Recursos Genéticos VegetaisCentro de Ciências AgráriasUniversidade Federal de Santa CatarinaRodovia Admar Gonzaga1346FlorianópolisSC 88034-000Brazil

Population genetics studies are widely recognized for generating useful knowledge for biodiversity *** date,however,little is known about the levels and distribution of genetic diversity of Drymis brasiliensis(Miers LC),a tree species from the Atlantic ***,in this study,we investigated how genetic diversity is distributed within and among populations of *** from southern Brazil using allozyme markers to genotype reproductive trees(8 populations)and seedlings(3 populations).Furthermore,in two populations,we established two permanent plots(5.1 and 1 ha)to analyze fine-scale genetic structure(FSGS).Studied populations presented low levels of genetic diversity(reproductive=0.085;seedlings=0.054)and high fixation indexes(reproductive=0.396;seedlings=0.231).Genetic divergence among populations was equal to 0.05,which is significant,signaling that few populations can conserve large portions of the species total genetic *** was only detected for one population,when reproductive individuals were separated by less than40 *** genetic diversity combined with high fixation indexes clearly signal a risk of losing ***,conservation efforts should be aimed at enhancing gene flow within the studied populations.

关键词： Atlantic Rainforest Effective population size Fine-scale genetic structure Seed collection

Localization of the Arabidopsis Senescence- and Cell Death-Associated BFN1 Nuclease： From the ER to Fragmented Nuclei

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Molecular Plant 2011年第6期4卷 1062-1073页

作者： Sarit Farage-Barhom Shaul Burd Lilian sonego Ana Mett Eduard Belausov David Gidoni Amnon Lers Department of Postharvest Science of Fresh Produce Volcani Center Agricultural Research Organization Bet Dagan 50250 Israe Faculty of Life Science Bar Ilan University Ramat Gan Israel Department of Vegetable Research Volcani Center Agricultural Research Organization Bet Dagan 50250 Israel Department of Ornamental Horticulture Volcani Center Agricultural Research Organization Bet Dagan 50250 Israe

Plant senescence- or PCD-associated nucleases share significant homology with nucleases from different organisms. However, knowledge of their function is limited. Intracellular localization of the Arabidopsis senescence- and PCD-associated nuclease BFN1 was investigated. Analysis of BFN1-GFP localization in transiently transformed tobacco protoplasts revealed initial localization in filamentous structures spread throughout the cytoplasm, which then clustered around the nuclei as the protoplasts senesced. These filamentous structures were identified as being of ER origin. In BFN1- GFP-transgenicArabidopsis plants, similar localization of BFN1-GFP was observed in young leaves, that is, in filamentous structures that reorganized around the nuclei only in senescing cells. In late senescence, BFN1-GFP was localized with fragmented nuclei in membrane-wrapped vesicles. BFNI＇s postulated function as a nucleic acid-degrading enzyme in senescence and PCD is supported by its localization pattern. Our results suggest the existence of a dedicated compartment mediating nucleic acid degradation in senescence and PCD processes.

关键词： BFN1 endoplasmic reticulum nuclease nuclear DNA programmed cell death senescence sub-cellular localization.

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p^H对高岭土高梯度磁选的影响

国外非金属矿与宝石 1991年第1期 14-17页

作者： maur.,CB 何国庆

Ziegler-Nichols Customization for Quadrotor Attitude Control under Empty and Full Loading Conditions

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Computer Modeling in Engineering & Sciences 2020年第10期125卷 65-75页

作者： Ivan Paulo Canal Manuel Martin Pérez Reimbold maur.cio de Campos Federal Institute of Education Science and Technology FarroupilhaCampus PanambiPanambiBrazil Regional University of the Northwest of the State of Rio Grande do Sul IjuíBrazil

An aircraft quadrotor is a complex control system that allows for great flexibility in *** multirotor aerial systems such as quadrotors is complex because the variables involved are not always available,known,and *** inclusion of payload changes the dynamic characteristics of the aircraft,making it necessary to adapt the control system for this *** the various control methods that have been investigated,proportional-integralderivative(PID)control offers good results and simplicity of application;however,achieving stability and high performance is challenging,with the most critical task being tuning the controller *** Ziegler-Nichols(ZN)theory was used to tune the controller gains for pitch and roll attitude command;however,the performance results were not *** response of this system was refined,resulting in an improvement in the reference tracking and the rejection of *** particular refinement was applied to the quadrotor,and via a reverse calculation,the parameters that allow the tuning of PID gains were obtained,based on *** particularization of the ZN theory applied to a quadrotor with and without a load(termed ZNAQ and ZNAQL,respectively)is proposed and results in a significant improvement in the control system response performance(up to 75%),demonstrating that ZNAQ and ZNAQL are valid for tuning the controller PID gains and are more efficient than the original ZN theory approach.

关键词： Ziegler-Nichols quadrotor control PID tuning multirotor

SUMOylation regulates p27^(Kip1) stability and localization in response to TGFβ

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分子细胞生物学报:英文版 2016年第1期8卷 17-30页

作者： Sara Lovisa Simona Citro maur. sonego Alessandra Dall’Acqua Valentina Ranzuglia Stefania Berton Alfonso Colombatti Barbara Belletti Susanna Chiocca Monica Schiappacassi Gustavo Baldassarre Division of Experimental Oncology 2 Centro di Riferimento Oncologico National Cancer InstituteAviano 33081Italy Department of Experimental Oncology European Institute of Oncology at the IFOM-IEO CampusMilan 20139Italy Department of Scienze e Tecnologie Biomediche MATI Center of ExcellenceUniversity of UdineUdine 33010Italy Present address:Department of Cancer Biology Metastasis Research CenterThe University of Texas MD Anderson Cancer CenterHoustonTX 77030USA.

Exposure of normal and tumor-derived cells to TGFbresults in different outcomes,depending on the regulation of key *** CDK inhibitor p27^(Kip1) is one of these TGFβ targets and is essential for the TGFβ-induced cell cycle *** treatment inhibits p27^(Kip1) degradation and induces its nuclear translocation,through mechanisms that are still *** evidences suggest that SUMOylation,a post-translational modification able to modulate the stability and subcellular localization of target proteins,critically modifies members of the TGFβ signaling ***,we demonstrate that p27^(Kip1) is SUMOylated in response to TGFβ *** different p27^(Kip1) point mutants,we identified lysine 134(K134)as the residue modified by small ubiquitin-like modifier 1(SUMO1)in response to TGFb ***β-induced K134 SUMOylation increased protein stability and nuclear localization of both endogenous and exogenously expressed p27^(Kip1).We observed thatSUMOylation regulated p27^(Kip1) binding to CDK2,thereby governing its nuclear proteasomal degradation through the phosphorylation of threonine ***,p27^(Kip1) SUMOylation was necessary for proper cell cycle exit following *** data indicate thatSUMOylation is a novel regulatory mechanism that modulates p27^(Kip1) function in response to TGFβ *** the involvement of TGFb signaling in cancer cell proliferation and invasion,our data may shed light on an important aspect of this pathway during tumor progression.

关键词： p^(27kip1) SUMOylation TGFβ

Growth,phenology and N-utilization by invasive populations of Gunnera tinctoria

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Journal of Plant Ecology 2020年第5期13卷 589-600页

作者： maur.cio Cruz Mantoani Alberto Benavent González Leopoldo García Sancho Bruce Arthur Osborne UCD School of Biology and Environmental Science and UCD Earth Institute University College DublinBelfieldDublin 4Ireland Departamento de Farmacología Farmacognosia y BotánicaFacultad de FarmaciaUniversidad Complutense de Madrid28040 MadridSpain

Aims Gunnera tinctoria is an unusual N-fixing plant species that has become invasive worldwide,generally in environments with a low evaporative demand and/or high *** the many mechanisms that may explain its success as an introduced species,a contrasting phenology could be important but this may depend on an ability to grow and utilize nutrients under sub-optimal *** examined whether *** has an advantage in terms of a contrasting phenology and N-fixing capability,in comparisons with Juncus effusus,the native species most impacted by *** *** We made phenological assessments on a weekly or bi-weekly basis on long-established populations on Achill Island,Ireland,during *** on leaf and inflorescence number,total leaf area,light interception and above-ground biomass were collected alongside measurements of soil temperature,moisture and oxidation-reduction *** significance of N-fixing ability for supporting seasonal growth was assessed usingδ15N isotopic assessments,together with in situ acetylene reduction *** Findings The timing of the initiation of growth of *** and *** varied between 2016 and 2017,with the earlier emergence and expansion of leaves of ***,and the largest above-ground biomass associated with higher water *** early growth of *** was dependent on preformed structures,with maximum canopy development occurring in late May,prior to that of *** N-fixation was observed in March,this made a more significant contribution to growth during the later stages of canopy *** onδ15N isotopic analyses,early growth was predominantly associated with N-remobilization from the rhizomes,whilst seedlings were largely reliant on *** emphasizes the importance of nutrient mobilization for early growth and shows that the importance of an N-fixing capability may vary developmentally,as well as during different

关键词： growth invasive species Gunnera tinctoria Juncus effusus nitrogen fixation