Immune response to inactivated bacterial vector carrying the recombinant K39 antigen of Leishmania infantum in mice

作     者：Lucelina S.Araújo Bruno B.Silva Eduarda N.F.N.Santos Arnaldo S.Bezerra Samuel S.Frota Assis R.Montenegro Eridan O.P.T.Florean Maurício Fvan Tilburg Maria Izabel F.Guedes Lucelina S.Araújo;Bruno B.Silva;Eduarda N.F.N.Santos;Arnaldo S.Bezerra;Samuel S.Frota;Assis R.Montenegro;Eridan O.P.T.Florean;Maurício F.van Tilburg;Maria Izabel F.Guedes

作者机构：Laboratory of Biotechnology and Molecular BiologyNortheast Biotechnology NetworkState University of CearáFortalezaBrazil Faculty of Veterinary MedicineState University of CearáFortalezaBrazil 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2024年第14卷第5期

页      面：199-206页

核心收录：

学科分类：1002[医学-临床医学] 100206[医学-皮肤病与性病学] 10[医学] 

基　　金：supported by grants from the Brazilian Agencies:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES-Financial code 001) Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq) Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico(FUNCAP) 

主　　题：Visceral leishmaniasis K39 Inactivated bacterial vector Vaccine Immune response Th1 Th2 Leishmania infantum 

摘      要：Objective:To evaluate the immunological response elicited by an inactivated bacterial vector carrying the K39 antigen of Leishmania infantum,and a purified ***:Mice were subjected to the following treatments:(1)Purified recombinant K39(rK39)protein at a 20μg dose with complete Freund’s adjuvant;(2)Inactivated Escherichia coli(BL21 DE3)carrying the K39 protein at an equivalent total protein content of 200μg;(3)Inactivated bacteria lacking the K39 protein;(4)Non-immunized control *** monitoring was *** groups were challenged by intraperitoneal injection of 10^(7) Leishmania infantum *** euthanasia,the liver and spleen were collected to analyze the levels of TNF,IFN-γ,IL-12,IL-4,and ***:Mice immunized with purified rK39 or the inactivated bacterial vector carrying the K39 antigen of Leishmania infantum showed a long-lasting immune response with high levels of polyclonal antibodies specifically recognizing the recombinant *** IgG1 subclass was the predominant immunoglobulin;however,the induction of IgG2a and the profile of cytokines produced were indicative of the induction of a mixed-type ***:The inactivated bacterial vector carrying the K39 antigen,as well as the purified antigen can induce a long-lasting immune response in immunized mice,predominantly favouring a Th2 profile response.