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Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

Phyton-International Journal of Experimental Botany 2023年第2期92卷 629-643页

作者： Alfaro-Corres Arnoldo Enrique Lecona-Guzman Carlos Alberto Gutiérrez-Miceli Federico Antonio martínez-esteves manuel Silverio-Gómez María del Carmen Medina-Lara María de Fátima Ruiz-Lau Nancy Laboratorio de Biotecnología vegetal Instituto Tecnológico de Tuxtla GutiérrezTuxtla Gutiérrez29050México Centro de Investigación Científica de Yucatán Unidad de Bioquímica y Biología Molecular de PlantasMérida97205México Insituto Nacional de Investigaciones Forestales Agrícolas y PecuariasHuimanguilloTabasco86400México CONACYT-Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez Tuxtla Gutiérrez29050México

The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana *** order to do the establishment of cells,the formation of callus was induced;for it two auxins were evaluated:2,4-dichlorophenoxyacetic acid(2,4-D)and 4-mino-3,5,6-trichloropicolinic acid(picloram)at three concentrations(0.25,0.5 and 0.75 mg L−1)in three explants(leaf,root and meristems)cultured in MS semisolid *** callogenesis response was related to the type and section of the explant,as well as the regulator used,and a cell suspension was established using 0.5 mg L−1 naphthaleneacetic acid(NAA)+0.5 mg L−1 Benzylaminopurine(BAP).Seedlings were exposed to polyethyleneglycol(15%and 30%w/v)with a water potential of−0.87 and−2.67 MPa,respectively,under soil *** stress was applied through restricted *** weight,root system growth,and chlorophyll concentration were some of the parameters that were affected by the effect of water deficit on *** *** concentration values were significantly decreased by 15 at 30%PEG(19.6 SPAD units)compared to the control *** in vitro plants,the highest concentration of proline was found in the roots,being the treatment with 30%polyethylene glycol where the highest concentration of this osmoregulator was obtained(62.5 mg g−1 DW).Under restricted irrigation conditions,an increase in proline concentration was observed both in the aerial part(2.2μg 100 g−1 DW)and in the root system(1.8μg 100 g−1 DW).However,the concentrations found were approximately ten times greater,less than those found under in vitro ***,the accumulation of proline can be considered an indicator of stress in Agave Americana *** in vitro.

关键词： Agave americana water deficit proline

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Amyloid-beta pathology-induced nanoscale synaptic disruption:the case of the GABA_B-GIRK assembly

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Neural Regeneration Research 2025年第5期20卷 1409-1410页

作者： Rafael Lujan Alejandro martín-Belmonte Sergi Ferré Francisco Ciruela Synaptic Structure Laboratory Instituto de Biomedicina de la UCLM(IB-UCLM)Departamento de Ciencias MédicasInstituto de Investigación en Salud de Castilla-La Mancha(IDISCAM)Facultad de MedicinaUniversidad Castilla-La ManchaCampus BiosanitarioC/AlmansaAlbaceteSpain Integrative Neurobiology Section National Institute on Drug AbuseIntramural Research ProgramNational Institutes of HealthBaltimoreMDUSA Pharmacology Unit Department of Pathology and Experimental TherapeuticsSchool of Medicine and Health SciencesInstitute of NeurosciencesUniversity of BarcelonaNeuropharmacology&Pain GroupNeuroscience ProgramBellvitge Institute for Biomedical ResearchL’Hospitalet de LlobregatSpain

Alzheimer's disease (AD) is characterized by an imbalance between excitatory and inhibitory brain networks,leading to aberrant homeostatic synaptic *** has progressively been recognized as syna ptopathy and syna ptic dysfunction has been identified as a key component of its pathogenesis (Schirinzi et al.,2020).Syna ptic dysfunction is believed to precede synapse loss,a primary biological correlate of cognitive decline in AD,inevita bly associated with neuronal death.

关键词： Alzheimer pathology

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5DGWO-GAN:A Novel Five-Dimensional Gray Wolf Optimizer for Generative Adversarial Network-Enabled Intrusion Detection in IoT Systems

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Computers, Materials & Continua 2025年第1期82卷 881-911页

作者： Sarvenaz Sadat Khatami Mehrdad Shoeibi Anita Ershadi Oskouei Diego martín Maral Keramat Dashliboroun Department of Data Science Engineering University of HoustonHoustonTX 77204USA The WPI Business School Worcester Polytechnic InstituteWorcesterMA 01609USA School of Systems and Enterprises Stevens Institute of TechnologyHobokenNJ 07030USA Department of Computer Science Escuela de Ingeniería Informática de SegoviaUniversidad de ValladolidSegovia40005Spain Ernest G.Welch School of Art&Design Georgia State UniversityAtlantaGA 30303USA

The Internet of Things(IoT)is integral to modern infrastructure,enabling connectivity among a wide range of devices from home automation to industrial control *** the exponential increase in data generated by these interconnected devices,robust anomaly detection mechanisms are *** detection in this dynamic environment necessitates methods that can accurately distinguish between normal and anomalous behavior by learning intricate *** paper presents a novel approach utilizing generative adversarial networks(GANs)for anomaly detection in IoT ***,optimizing GANs involves tuning hyper-parameters such as learning rate,batch size,and optimization algorithms,which can be challenging due to the non-convex nature of GAN loss *** address this,we propose a five-dimensional Gray wolf optimizer(5DGWO)to optimize GAN *** 5DGWO introduces two new types of wolves:gamma(γ)for improved exploitation and convergence,and theta(θ)for enhanced exploration and escaping local *** proposed system framework comprises four key stages:1)preprocessing,2)generative model training,3)autoencoder(AE)training,and 4)predictive model *** generative models are utilized to assist the AE training,and the final predictive models(including convolutional neural network(CNN),deep belief network(DBN),recurrent neural network(RNN),random forest(RF),and extreme gradient boosting(XGBoost))are trained using the generated data and AE-encoded *** evaluated the system on three benchmark datasets:NSL-KDD,UNSW-NB15,and *** conducted on diverse IoT datasets show that our method outperforms existing anomaly detection strategies and significantly reduces false *** 5DGWO-GAN-CNNAE exhibits superior performance in various metrics,including accuracy,recall,precision,root mean square error(RMSE),and convergence *** proposed 5DGWO-GAN-CNNAE achieved the lowest RMSE values across the NSL-KDD,UNSW-NB15,and

关键词： Internet of things intrusion detection generative adversarial networks five-dimensional binary gray wolf optimizer deep learning

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Influence of steel jacket thickness on the RC bridges' seismic vulnerability

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Journal of Traffic and Transportation Engineering(English Edition) 2019年第1期6卷 15-34页

作者： Bertha Olmos José manuel Jara Gerardo Gómez Guillermo martínez Department of Civil Engineering Universidad Michoacana de San Nicolas de Hidalgo

The seismic demands of bridges subjected to strong earthquakes are mostly concentrated on piers. The main objective of this study is to assess the seismic vulnerability of retrofitted bridges with steel jackets. It analyzes the influence of the steel jacket thickness on the seismic capacity and demand of bridges. The bridges in study have two simple supported spans with superstructure composed by AASHTO type girders and a substructure formed with one circular pier over a stiff foundation. They are located on the Pacific coast of Mexico that is a zone of high seismicity. The bridges seismic capacity are evaluated through nonlinear static analyses and the shear force and displacement demands were determined using capacity spectra with a group of 10 real accelerograms from a subduction source. The response spectra were scaled to several peak ground accelerations(PGA) with the aim to estimate four damage scenarios linked each one to a probability of exceedance in a specific period, for return periods in the range of 72-3500 years that represent service level and maximum considered earthquake. Results show that the confining effect provided by the steel jackets substantially improves the seismic performance of the bridges piers. The increase of jacket thickness guides to important increments on columns base shear capacity, and the influence of the steel jacket thickness depends on the limit state and bridge slenderness ratio.

关键词： Steel jacket Piers’retrofit Subduction earthquakes RC piers’vulnerability

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Repellent and Insecticidal Activity of Naphthoquinones from the Heartwood of Tectona grandis on Incisitermes marginipennis(Latreille)

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2165-2181页

作者： Crisanto Velázquez-Becerra Gu Yen Ambriz-Ortiz Rafael Torres-martínez Mauro manuel martínez-Pacheco Facultad de Ingeniería en Tecnología de la Madera Universidad Michoacana de San Nicolás de HidalgoMoreliaMéxico Escuela Nacional de Estudios Superiores Unidad MoreliaMoreliaMéxico Instituto de Investigaciones Químico-Biológicas Universidad Michoacana de San Nicolás de HidalgoMoreliaMéxico

Wood is a lignocellulosic material that,because of its organic nature,is a source of nutrients for bacteria,fungi,and *** marginipennis(Latreille)is an interesting dry wood-boring *** it is an extremely destructive pest,difficult to control due to its cryptic lifestyle,and for its role in the biogeochemical cycle of carbon and other elements;it is a fundamental *** use of less toxic and eco-friendly substances is important for protecting domestic,cultural,and historical heritage made of wood or lignocellulosic *** aim of this study was to evaluate the biological activity of various naphthoquinones extracted from the heartwood of *** on ***,heartwood flour was used for Soxhlet-type extractions to isolate and characterize the heartwood compounds by thin-layer *** identify the heartwood compounds,nuclear magnetic resonance and gas chromatography coupled to a mass spectrometer were *** information obtained was supported using the Interactive Spectroscopy *** identified heartwood compounds were lapachol,tectoquinone,deoxylapachol,dehydro-α-lapachone,tectol and *** nymphal termites of *** as a biological model,the isolated and identified molecules were under three different tests(repellent,insecticide and protective)and their biological activity was individually *** results showed that the molecules obtained have a repellent-protective effect against ***,although not in all cases,these molecules have high percentages of mortality and decreased the weight loss of the treated material exposed to ***.

关键词： Naphthoquinones dry wood termite Incisitermes marginipennis wood preservatives

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Neural Regeneration Research 2021年第1期16卷 123-124页

作者： Carlos Bueno Salvador martínez Instituto de Neurociencias de Alicante(UMH-CSIC) San JuanAlicanteSpain

Human neurological disorders and spinal cord injuries are caused by a loss of neurons and glial cells in the brain or spinal *** proof-ofprinciple of cell therapy is that replacing damaged cells with new healthy ones will restore the lost *** and manipulating autologous neural stem cells(NSCs)could provide an ideal source of cells for use in cell replacement and the transfer of genes to a diseased central nervous *** are immature cells present,not only during embryonic development,but also in the adult brain of all mammalian species,including *** presence of NSCs in the adult mammalian brain has been described in two neurogenic niches,the ventricular-subventricular zone(V-SVZ)of the anterolateral ventricle wall and the subgranular zone of the hippocampal dentate ***,NSCs are defined as self-renewing multipotent cells that can generate neurons,astrocytes and *** major barrier to isolating adult NSCs in humans is the inaccessibility of living *** this reason,an enormous effort has been made to derive neurons from human adult stem cells isolated from various tissue sources.

关键词： injuries ventricular adult

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Importance of Quercus *** diversity and biomass of vascular epiphytes in a managed pine-oak forest in Southern Mexico

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Forest Ecosystems 2022年第3期9卷 366-377页

作者： Nayely martínez-Meléndez Neptalí Ramírez-Marcial José G. García-Franco manuel Jesús Cach-Pérez Pablo martínez-Zurimendi Departamento de Conservacion de la Biodiversidad El Colegio de la Frontera Sur(ECOSUR)San Cristobal de Las Casas29290ChiapasMexico Red de Ecología Functional Instituto de EcologíaA.C.Xalapa91073VeracruzMexico Departamento de Agricultura Sociedad y AmbienteEl Colegio de la Frontera Sur(ECOSUR)Villahermosa86280TabascoMexico

Background:Forestry management modifies the diversity,structure,and functioning of intervened *** extraction reduces tree density and basal area,leading to changes in the communities of vascular *** objective of this study was to evaluate the diversity and biomass of vascular epiphytes in Quercus trees remaining in two pine-oak forest stands that have been subjected to two stages of the Silvicultural Development Method(release cutting,and thinning)in comparison with an unharvested old-secondary forest in southern *** each treatment,richness of epiphytes present on 60 oak trees was recorded and their dry biomass *** calculated the true diversity(Hill numbers)and beta diversity using the Jaccard coefficient of similarity,and generated rank abundance curves per taxonomic epiphyte group(bromeliads,orchids,ferns and others).For each treatment,the relationships between overall diversity and epiphyte biomass to the host trees basal area were analyzed using log linear ***:We recorded a total of 67 species of epiphytes species belonging to 10 families hosted by five species of *** greatest species richness(^(0)D)was recorded in the old-secondary *** common(^(1)D)and dominant(^(2)D)species were recorded in the release cutting than in the other *** diversity and biomass were both slightly related to host tree basal *** of epiphytes was similar(60%)among treatments,although orchids,bromeliads,and other families were more diverse in the old-secondary *** bromeliad species were shared across all treatments,although orchids presented the most exclusive species in the unharvested *** bromeliad Tillandsia seleriana provided the greatest contribution to biomass in all treatments,followed by the orchid Camaridium *** linear models indicated that epiphyte diversity was significantly related to treatment,and epiphyte biomass to basal area of host ***:Alt

关键词： Bromeliads Chiapas Ferns Orchids Richness Sierra Madre Silvicultural development method(SDM)

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Tongluo Jiedu as an adjuvant therapy for oral cancer

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World Journal of Clinical Cases 2025年第5期13卷 52-57页

作者： Sarah Monserrat Lomelímartínez Melissa martínez Nieto Ana Esther Mercado González Department of Medical and Life Sciences Centro Universitario de la CiénegaUniversidad de GuadalajaraOcotlán 47810Mexico Department of Well-being and Sustainable Development Centro Universitario del NorteUniversidad de GuadalajaraColotlan 46200Mexico Independent Researcher Guadalajara 44340Mexico Antiguo Hospital Civil de Guadalajara“Fray Antonio Alcalde” Universidad de GuadalajaraGuadalajara 44280Mexico

Oral cancer is one of the malignant neoplasms that present major global health *** is the sixth most prevalent type of cancer in the world,with a high incidence and mortality *** letter is a review of the study by Yin et al which was published in the World Journal of Clinical Cases(2024).The study evaluated the effect of Tongluo Jiedu as an adjuvant treatment for oral *** the years,there has been a continuous search for effective and less invasive treatments for oral *** article emphasizes and discusses various therapeutic options currently available,and it highlights that early intervention and multidisciplinary management are crucial for improving *** Chinese medicine,particularly Tongluo Jiedu,presents potential complementary approach to co-nventional oral cancer *** research on Tongluo Jiedu should be focused on validation of its efficacy and safety through large,well-designed clinical trials,as well as better understanding of the molecular mechanisms in-volved,and optimization of therapeutic ***,continuous education of health professionals is key to the effective and safe integration of this traditional medicine into clinical *** research is essential for optimization of therapeutic strategies and for addressing the challenges presented by this neoplasm.

关键词： Oral cancer Tongluo Jiedu Traditional Chinese medicine Chinese herbal medicine Herbal drugs Dentistry

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Proteomic profile of human monocytic cells infected with dengue virus

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Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 914-923页

作者： Viviana martínez-Betancur Marleín martínez-Gutierrez Programa de Estudio y Control de Enfermedades Tropicales-PECET Universidad de Antioquia Grupo de Investigación en Ciencias Animales-GRICA Facultad de Medicina Veterinaria y Zootecnia Universidad Cooperativa de Colombia

Objective: To identify the changes in the proteome of U937 cells infected with dengue virus(DENV).Methods: In this study, differentiated U937 cultures were infected with two DENV-2strains, one of which was associated with dengue(DENV-2/NG) and the other one with severe dengue(DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein ***: In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially(five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed(two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 k Da protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 k Da protein AA1, tubulin beta, enolase 1, pyruvate kinase,transaldolase and phospholipase ***: Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection.

关键词： Dengue virus Proteomic Macrophages Monocytes U937 cells

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An Evolutionary Approach to the Delineation of Functional Areas Based on Travel-to-work Flows

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International Journal of Automation and computing 2008年第1期5卷 10-21页

作者： Francisco Flórez-Revuelta Josémanuel Casado-Díaz Lucas martínez-Bernabeu Department of Computing Technology University of AlicanteP.O.Box 99E-03080AlicanteSpain Institute of International Economics University of AlicanteP.O.Box 99F-03080AlicanteSpain

This paper presents a new approach to the delineation of local labor markets based on evolutionary computation. The aim of the exercise is the division of a given territory into functional regions based on travel-to-work flows. Such regions are defined so that a high degree of inter-regional separation and of intra-regional integration in both cases in terms of commuting flows is guaranteed. Additional requirements include the absence of overlap between delineated regions and the exhaustive coverage of the whole territory. The procedure is based on the maximization of a fitness function that measures aggregate intra-region interaction under constraints of inter-region separation and minimum size. In the experimentation stage, two variations of the fitness function are used, and the process is also applied as a final stage for the optimization of the results from one of the most successful existing methods, which are used by the British authorities for the delineation of travel-to-work areas （TTWAs）. The empirical exercise is conducted using real data for a sufficiently large territory that is considered to be representative given the density and variety of travel-to-work patterns that it embraces. The paper includes the quantitative comparison with alternative traditional methods, the assessment of the performance of the set of operators which has been specifically designed to handle the regionalization problem and the evaluation of the convergence process. The robustness of the solutions, something crucial in a research and policy-making context, is also discussed in the paper.

关键词： Evolutionary computation regional science economics optimization methods

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