Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

作     者：Alfaro-Corres Arnoldo Enrique Lecona-Guzman Carlos Alberto Gutiérrez-Miceli Federico Antonio Martínez-Esteves Manuel Silverio-Gómez María del Carmen Medina-Lara María de Fátima Ruiz-Lau Nancy 

作者机构：Laboratorio de Biotecnología vegetalInstituto Tecnológico de Tuxtla GutiérrezTuxtla Gutiérrez29050México Centro de Investigación Científica de YucatánUnidad de Bioquímica y Biología Molecular de PlantasMérida97205México Insituto Nacional de Investigaciones ForestalesAgrícolas y PecuariasHuimanguilloTabasco86400México CONACYT-Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla GutiérrezTuxtla Gutiérrez29050México 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2023年第92卷第2期

页      面：629-643页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：To CONACYT for the Master’s scholarship of Arnoldo Enrique Alfaro Corres(Scholarship No.621617) 

主　　题：Agave americana water deficit proline 

摘      要：The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana *** order to do the establishment of cells,the formation of callus was induced;for it two auxins were evaluated:2,4-dichlorophenoxyacetic acid(2,4-D)and 4-mino-3,5,6-trichloropicolinic acid(picloram)at three concentrations(0.25,0.5 and 0.75 mg L−1)in three explants(leaf,root and meristems)cultured in MS semisolid *** callogenesis response was related to the type and section of the explant,as well as the regulator used,and a cell suspension was established using 0.5 mg L−1 naphthaleneacetic acid(NAA)+0.5 mg L−1 Benzylaminopurine(BAP).Seedlings were exposed to polyethyleneglycol(15%and 30%w/v)with a water potential of−0.87 and−2.67 MPa,respectively,under soil *** stress was applied through restricted *** weight,root system growth,and chlorophyll concentration were some of the parameters that were affected by the effect of water deficit on *** *** concentration values were significantly decreased by 15 at 30%PEG(19.6 SPAD units)compared to the control *** in vitro plants,the highest concentration of proline was found in the roots,being the treatment with 30%polyethylene glycol where the highest concentration of this osmoregulator was obtained(62.5 mg g−1 DW).Under restricted irrigation conditions,an increase in proline concentration was observed both in the aerial part(2.2μg 100 g−1 DW)and in the root system(1.8μg 100 g−1 DW).However,the concentrations found were approximately ten times greater,less than those found under in vitro ***,the accumulation of proline can be considered an indicator of stress in Agave Americana *** in vitro.