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Antioxidant,Antibacterial and Antitumor Activity of Ethanolic Extract of the Psidium guajava Leaves

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American Journal of Plant Sciences 2014年第23期5卷 3492-3500页

作者： Tatiane Vieira Braga Rosana Goncalves Rodrigues das Dores Camila Soncin Ramos Fernanda Cristina Gontijo Evangelista Leticia Marcia da Silva Tinoco Fernando de Pilla Varotti maria das gracas Carvalho Adriano de Paula Sabino Federal University of Minas Gerais Belo HorizonteBrazil Federal University of Ouro Preto Ouro PretoBrazil Federal University of Sao Joao Del Rei DivinopolisBrazil Pitagoras Faculty Belo HorizonteBrazil

Psidium guajava L. has extensive use in folk medicine. The aim of this study was to quantify the levels of phenolic, flavonoids, antioxidant activity, leathality assay and antibacterial and antitumoral activities of the extract of P. guajava. In the dry guava extract there were high levels of phenolics (766.08 ± 14.52 mg/g), flavonoids (118.90 ± 5.47 mg/g) and antioxidant activity (87.65%). The LD50 was 185.15 μg/ml. The MIC value was 250 μg/ml for Streptococcus mutans, Streptococcus mitis and Streptococcus oralis. IC50 of the extract tested in the HeLa, RKO and Wi cell lines was 15.6 ± 0.8 μg/ml, 21.2 ± 1.1 μg/ml and 68.9 ± 1.5 μg/ml, respectively. The results of all analyses allow us to conclude that the dry extract of guava leaves has promising activity to be applied topically in the oral cavity or in the development of antitumor formulation or even be used as a functional food.

关键词： Guava Antitumor Medicinal Plants Functional Foods

Quality of Melon 'UFERSA-05 *** and Pele de Sapo“Mabel”Minimally Processed,Kept in Different Packages

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Food and Nutrition Sciences 2014年第8期5卷 683-691页

作者： maria Luiza Pereira de Araújo Fanquiele Ribeiro de Oliveira marianne Lima Barboza Glauber Enrique Souza nunes Pahlevi Augusto de Souza Adriano do Nascimento Simoes Unidade Academica de Serra Talhada Universidade Federal Rural de PernambucoSerra TalhadaBrazil Departamento de Ciencias Vegetais UFERSAMossoróBrazil Instituto Federal de Educacao Ciencia e Tecnologia do CearáLimoeiro do NorteBrazil

This study aimed at evaluating the quality of melon Pele de Sapo “Mabel” and Yellow melon “UFERSA-05” minimally processed preserved in different packages. The fruits were harvested at Mossoró-RN and transported to Serra Talhada-PE. In the first study, melon fruit Piel de Sapo “Mabel” was selected, washed, weighed, cooled, peeled, cut into cube shape, sanitized in chlorine solution, drained, packaged in rigid polypropylene tray, sealed with polypropylene film or polypropylene rigid cap and kept for 8 days at 8℃. In the second study, fruits of yellow melon “UFERSA-05” and Pele de Sapo “Mabel” were minimally processed and kept in rigid polypropylene tray, sealed with polypropylene film and stored for 8 days at 8℃. There was no significant interaction between packaging (sealed with film and polypropylene cover) and period of conservation, for pH, total titratable acidity (TTA), total soluble solids (TSS) and Loss of fresh mass (LFM) for Melon “Mabel”. While there was significant interaction between types of melon (“UFERSA-05” and “Mabel”) and storage period (0, 2, 4, 6, and 8 days) for pH, TTA and TSS. In sensory evaluation were noticeable changes in appearance, flavor, aroma and flesh firmness, characterized by translucency, alcoholic aroma and softening in “Mabel” melon kept in tray with lid. Melon “UFERSA-05” showed lower pH, total soluble solids, total soluble sugars, PME activity, lack of translucency high flesh firmness compared to “Mabel” melon during storage. The high levels of sugars in melon “Mabel” may be related to the incidence of translucency, which was not observed in “UFERSA-05” melon, with a strong potential to minimal processing.

关键词： Cucumis melo Translucency Pectin Methylesterase Total Soluble Sugars

“Cachacas”(Sugarcane Spirit)Aged Quantitation of Phenolic Compounds,Antibacterial and Antioxidant Activity

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American Journal of Plant Sciences 2014年第20期5卷 2935-3942页

作者： Leonardo Milani Avelar Rodrigues maria das gracas Cardoso Luís Roberto Batista Wilder Douglas Santiago Juliana maria Valério Resende Juliana de Andrade Santiago Marcos de Souza Gomes Milene Aparecida Andrade maria Luisa Teixeira Fabiana Reinis Franca Passamani Department of Food Science Federal University of LavrasLavrasBrazil Department of Chemistry Federal University of LavrasLavrasBrazil

The “cachaça” is currently one of the fastest growing agro-industrial industries in the country as the drink considered as a symbol of Brazilian nationality. This study aimed to quantify the phenolic compounds, evaluate the antioxidant and antibacterial activity of different samples of aged “cachaça”. The physic-chemical analysis was performed at the Brandy Quality Laboratory of the Federal University of Lavras, and the determination of phenolic compounds was performed by High Performance Liquid Chromatography (HPLC). The antimicrobial activity evaluation was held at the Food Mycology Laboratory through the cavity agar diffusion technique and by steam using the micro-organisms Escherichia coli, Staphylococcus aureus, Salmonella choleraesuis, Listeria monocytogenes and Pseudomonas aeruginosa. The antioxidant activity was evaluated using the DPPH radical sequestering method. The values obtained for the phenolic compounds ranged from 0.41 to 9.69 mg/L, observing the predominance of syringaldehyde, gallic acid and vanillic acid. The phenolic extracts showed a satisfactory antibacterial activity for both gram-negative and gram-positive but did not show inhibition against the bacteria Pseudomonas aeruginosa. The antioxidant activity was evidenced by the DPPH test for cachaças tested and the “cachaça” aged in oak barrel showed the highest activity, followed by “cachaça” aged in jequitibá, amburana and balm barrels.

关键词： Syringaldehyde Gallic Acid Vanillic Acid Bacteria DPPH Test

Phytometric and Productive Characteristics of Safflower Submitted to Phosphate Fertilization in the Oxisol of the Brazilian Cerrado

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American Journal of Plant Sciences 2017年第12期8卷 2966-2976页

作者： Edna maria Bonfim-Silva Luiz Fernando Santos Miranda Laura Cristina Rezende das Neves Helon Hébano de Freitas Sousa Jefferson Vieira José Department of Agricultural Engineering Federal University of Mato Grosso Rondonópolis Brazil

The safflower (Carthamus tinctorius L.), in particular, has been soaring in significance as an alternative for oil and biodiesel production. In this context, the goal of this study was to assess the influence exerted by phosphate fertilization on the phytometric and productive characteristics of the safflower raised on the Oxisol of the Cerrado. The experiment was performed in a greenhouse, using pots of 3 dm-3?in volume. Adopting the completely randomized design, five doses of phosphorus (P2O5: 0, 90, 180, 360 and 540 mg·dm-3) and six replicates were included. The following variables were estimated, viz., number of leaves, plant height, and stem diameter, besides chlorophyll index (SPAD index), number of chapters and shoot dry matter. Barring the shoot dry matter and number of chapters, all the other variables were evaluated on days 30 and 45 after the plants were transplanted. Data were submitted to analysis of variance at 1%, 5% and 10% probabilities, and regression analysis, at 1% and 5% probabilities, respectively. All the variables revealed significant differences and adjustments to the linear regression model (P < 0.1), excepting the SPAD index on day 30 after the plant emergence which was adjusted to the quadratic regression model. The highest findings of 24.47 for leaf number, 34.97 cm for plant height, 3.76 mm for stem diameter, 64.76 for chlorophyll index, 5 units for the number of chapters and 3.45 g for dry shoot mass were recorded in response to the highest phosphorus dose in the experimental range (P2O5: 540 mg·dm-3). Phosphate fertilization was observed to affect the phytometric characteristics and boost the development of the safflower plants cultivated in the Cerrado Oxisol, substantiating that safflower could be a viable cash crop that can be established in the Brazilian Cerrado.

关键词： Carthamus tinctorius L. Alternative Culture for Biodiesel Oleaginous

Triterpenes from Cabralea canjerana as in Vitro Inhibitors to Light Reactions of Photosynthesis

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American Journal of Plant Sciences 2014年第16期5卷 2528-2540页

作者： Beatriz King-Diaz Marcio S.Soares maria Fatima das G.F.da Silva Blas Lotina-Hennsen Thiago A.M.Veiga Departamento de Bioquimica Facultad de QuimicaUniversidad Nacional Autonoma de Mexico(UNAM)Ciudad UniversitariaMexico D.F.México Universidade Federal de Vicosa(UFV) Rio ParanaibaBrasil Departamento de Química Universidade Federal de Sao Carlos(UFSCar)Sao CarlosBrasil Instituto de Ciencias Ambientais Químicas e FarmaceuticasDepartamento de Ciencias Exatas e da TerraUniversidade Federal de Sao Paulo(UNIFESP)DiademaBrasil

Aims: Cabraleadiol (1), Ocotilone (2) and Odoratone (3) are three triterpenes isolated from Cabralea canjerana (Vell.) Mart. (Meliaceae). They were chemically characterized, and their effect was tested on the light reaction of photosynthesis. Study Design: Natural products were used as models to find new models for inhibitors of photosynthesis. Place and Duration of Study: Departmento de Química, Universidade Federal de São Carlos and Departmento de Bioquímica, Facultad de Química, Universidad Nacional Autonoma de México, between May 2013 and January 2014. Methodology: The natural products had their effect on the light reaction of photosynthesis studied by pollarography and Chlorophyll a (Chl a) fluorescence transients. Results: The compounds inhibited ATP synthesis and electron transport rate (basal, phosphorylating and uncoupled). Therefore, they act as Hill reactions inhibitors. Their inhibition site were located in the range of electron flow from OEC complex and between P680 to QA of PS II, and inhibited the photosystem II (PS II) by inducing the appearance of a K-band which is an indicative that the photochemical apparatus is failing at the donor side of PS II interacting at the OEC complex and by transforming active reaction centers to “heat sinks” or the formation of silent reaction centers unable to reduce QA. Conclusion: Furthermore, these triterpenes inhibit PS II and induce the appearance of small G band which is related with the decreased plastoquinone (PQ) pool reduction.

关键词： Cabralea canjerana Cabraleadiol Ocotilone Odoratone Photosynthesis

Risk factors associated with inflammatory bowel disease: A multicenter case-control study in Brazil

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World Journal of Gastroenterology 2020年第25期26卷 3611-3624页

作者： Valéria Cristina Loureiro Salgado Ronir Raggio Luiz Neio Lucio Fernandes Boéchat Isabella Sued Leao Bianca do Carmo Schorr JoséMiguel Luz Parente Daniela Calado Lima Eduardo Santos Silveira Júnior Genoile Oliveira Santana Silva Neogélia Pereira Almeida Andrea Vieira maria Luiza Queiroz de Bueno Júlio maria Chebli érika Ruback Bertges Luísa Martins da Costa Brugnara Columbano Junqueira Neto Stefania Burjack Gabriel Campbell Luana Letiza Discacciati Joao Paulo Silva Cézar Tiago nunes Gilaad G Kaplan Cyrla Zaltman Division of Gastroenterology Department of Internal MedicineClementino Fraga Filho HospitalFederal University of Rio de JaneiroRio de Janeiro 21940-230Brazil Institute for Studies in Public Health Federal University of Rio de JaneiroRio de Janeiro 21940-230Brazil Multidisciplinary Research Laboratory Clementino Fraga Filho HospitalInstitute of Thoracic DiseasesFaculty of MedicineFederal University of Rio de JaneiroRio de Janeiro 21940-230Brazil Division of Gastroenterology Department of Internal MedicineHospital UniversityFaculty of MedicineFederal University of PiauíPiauí64049-550Brazil Almeida Division of GastroenterologyInflammatory Bowel Disease Outpatient ClinicRoberto Santos General Hospital(HGRS)of the Bahia State Department of HealthBahia 40110-060Brazil Division of Gastroenterology Inflammatory Bowel Disease Outpatient ClinicIrmandade Santa Casa da Misericórdia of São PauloSão Paulo 01221020Brazil Division of Gastroenterology Department of Internal MedicineHospital UniversityFaculty of MedicineFederal University of Juiz de ForaMinas Gerais 36036-247Brazil Division of Gastroenterology Inflammatory Bowel Disease Outpatient ClinicFederal District Base HospitalBrasília 70330-150Brazil Gastrointestinal Physiology Institute of Nutritional ScienceNestle Research CenterLausanne 1000Switzerland Departments of Medicine and Community Health Sciences University of CalgaryCalgary T2N4Z6Canada

BACKGROUND The etiology of inflammatory bowel disease(IBD)is unknown,but it is believed to be *** hygiene hypothesis proposes that better hygiene conditions would lead to less infectious disease during childhood and favor the development of immune-mediated *** To test the hygiene hypothesis in IBD by assessing the environmental risk factors associated with IBD development in different regions of Brazil with diverse socioeconomic development *** A multicenter case-control study was carried out with 548 Crohn’s disease(CD)and 492 ulcerative colitis(UC)outpatients and 416 healthy controls,from six IBD centers within different Brazilian states at diverse socioeconomic development stages.A semi-structured questionnaire with 87 socioeconomic and environmental questions was *** regression model was created to assess the odds ratio(OR)with P value and 95%confidence intervals(CI).RESULTS Predictive variables for both diseases(CD and UC)were women[odd ratios(OR)=1.31;OR=1.69],low monthly family income(OR=1.78;OR=1.57),lower number of cohabitants(OR=1.70;OR=1.60),absence of vaccination(OR=3.11;OR=2.51),previous history of bowel infections(OR=1.78;OR=1.49),and family history of IBD(OR=5.26;OR=3.33).Associated risk factors for CD were age(18-39 years)(OR=1.73),higher educational level(OR=2.22),absence of infectious childhood diseases(OR=1.99).The UC predictive variables were living in an urban area(OR=1.62),inadequate living conditions(OR=1.48)and former smokers(OR=3.36).Appendectomy was a risk factor for CD(OR=1.58)with inverse association with UC(OR=4.79).Consumption of treated and untreated water was associated with risk of CD(OR=1.38)and UC(OR=1.53),*** This is the first examining environmental exposures as risk factors for inflammatory bowel disease in *** of the variables associated with disease risk support the role of the hygiene hypothesis in IBD development.

关键词： Crohn’s disease Ulcerative colitis Risk factors Environmental factors Hygiene hypothesis

High prevalence of vitamin A deficiency in Crohn's disease patients according to serum retinol levels and the relative dose-response test

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World Journal of Gastroenterology 2015年第5期21卷 1614-1620页

作者： Márcia Soares-Mota Tianny A Silva Luanda M Gomes Marco AS Pinto Laura MC Mendon?a maria Lúcia F Farias Tiago nunes Andrea Ramalho Cyrla Zaltman Instituto de Nutricao Josué de Castro Federal University of Rio de JaneiroRio de Janeiro 21941-913Brazil Departamento de Clínica Médica Hospital Universitário Clementino Fraga FilhoFederal University of Rio de JaneiroRio de Janeiro 21941-901Brazil Nutrition and Immunology Chair ZIEL Research Center for Nutrition and Food SciencesTechnical University of MünchenFreising-Weihenstephan80333 MünchenGermany

AIM:To assess the vitamin A status of patients with Crohn's disease(CD) by evaluating serum retinol levels and the relative dose response(RDR) test(liver retinol stores).METHODS:Vitamin A nutritional status was measured by serum retinol obtained by high performance liquid chromatography and the RDR test for evaluation of the hepatic *** composition was performed by densitometry by dual-energy X-ray *** A dietary intake was assessed from a semiquantitative food frequency ***:This study included 38 CD patients and 33 *** serum retinol concentrations were detected in 29% of CD patients vs 15% in controls(P < 0.005).The RDR test was positive in 37% of CD patients vs 12% in controls,which indicated inadequate hepatic vitamin A stores(P < 0.005).Individuals with hypovitaminosis A had lower BMI and body fat compared with those without this *** was no association between vitamin A deficiency and its dietary intake,ileal location,presence of disease activity and prior bowel ***:Patients with CD have higher prevalence of vitamin A deficiency,as assessed by two independent methods.

关键词： Crohn's disease Vitamin A Serum retinol Relative dose response test Body composition

Morphophysiology of Germination among Seeds of Pau-rainha （Centrolobium paraense） Collected in the State of Roraima

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Journal of Agricultural Science and Technology(B) 2016年第6期6卷 371-379页

作者： Luis Augusto Melo Schwengber Oscar Jose Smiderle Aline das gracas Souza Jose maria Arcanjo Alves Dalton Roberto Schwengber Cylles Zara dos Reis Barbosa Postgraduate Program in Agronomy Universidade Federal de Roraima (UFRR) Boa Vista RR CEP 69300-000 Brazil Brazilian Agricultural Research Corporation--Embrapa Roraima Boa Vista RR CEP 69301-970 Brazil Laboratory of Plant Tissue Culture Federal University of Pelotas Campus Capao do Leao RS CEP 96010-900 Brazil

Population Dynamics of Croton blanchetianus *** a Caatinga Area in the Brazilian Semi-Arid

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American Journal of Plant Sciences 2018年第4期9卷 920-932页

作者： Alecksandra Vieira de Lacerda Joao Paulo Pereira de Lima Tarcizio Jacinto de Oliveira nunes Azenate Campos Gomes Carina Seixas Maia Dornelas Hugo Morais de Alcantara Francisca maria Barbosa Center for the Sustainable Development of the Semi-Arid Federal University of Campina GrandeSuméParaíbaBrazil Health Sciences Center Federal University of ParaíbaJoao PessoaParaíbaBrazil National Institute of Science Technology—Northeast North Fitoproducts Networkplant Products Joao PessoaParaíbaBrazil

The vegetation of the Caatinga, present in the dry areas of the Brazilian Semi-arid region has been little studied, when it relates to population dynamics of their species. The objective of this study was to evaluate the mortality and recruitment rates of a Croton blanchetianus Baill. population in a two-year interval (January/2013 to January/2015), in area of caatinga in the semi-arid Paraiba. Monitoring of structural changes in the vegetation was carried out in 96 10 × 10 m contiguous plots. The phytosociological survey conducted in January 2013 sampled 1078 individuals of C. blanchetianus, occurring in the 96 plots studied. The years of 2012 and 2013 had rainfall below the annual average. Take into consideration the evaluation period;there was a high mortality rate (M = 47.33% year-1) and a low recruitment rate (R = 0.18% year-1) for the population of C. blanchetianus. The real gain (RG) of the population was significantly negative (-93.91% year-1) since the number of dead individuals was much higher than the recruited ones in the population. The highest height class, composed of individuals of 4.1 to 5.0 m height, was the most tolerant to water scarcity, resulting in 53.85% of surviving individuals. Therefore, the results demonstrated that periodic droughts compromise the recruitment, development, survival, and establishment of individuals in natural systems in the Brazilian semi-arid region.

关键词： Marmeleiro Populational Structure Ecosystemic Dynamic Seasonality Dry Forests