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AMP-ac.ivated kinase in human spermatozoa： identific.tion, intrac.llular loc.lization, and key func.ion in the reRulation of sperm motility

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Asian Journal of Andrology 2017年第6期19卷 707-714页

作者： Violeta c.lle-Guisado Ana Hurtado de Llera David Martin-Hidalgo Jose Mijares maria c.Gil Ignac.o S Alvarez maria J Bragado Luis J Garc.a-Marin Researc. Group of Intrac.llular Signalling and Tec.nology of Reproduc.ion (SINTREP) School of Veterinary Medicine University of Extremadura Caceres Spain Assisted Reproduc.ion Unit at the-Minimally Invasive surgery c.nter Jesos Uson (c.MIJU) c.c.res Spain Norba c.inic Caceres Spain Researc. Group of Reproduc.ion and Embryo Development (REDES) University of Extremadura Badajoz Spain Extremadura Institute of Assisted Reproduc.ion (IERA) Badajoz Spain.

AMP-ac.ivated kinase （AMPK）, a protein that regulates energy balanc. and metabolism, has rec.ntly been identified in boar spermatozoa where regulates key func.ional sperm proc.sses essential for fertilization. This work＇s aims are AMPK identific.tion, intrac.llular loc.lization, and their role in human spermatozoa func.ion. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Thr172-AMPK were analyzed by Western blotting and indirec. immunofluoresc.nc.. High- and low-quality sperm populations were separated by a 40%-80% density gradient. Human spermatozoa motility was evaluated by an Integrated Semen Analysis System （ISAS） in the presenc. or absenc. of the AMPK inhibitor c.mpound c.（c.）. AMPK is loc.lized along the human spermatozoa, at the entire ac.osome, midpiec. and tail with variable intensity, whereas its ac.ive form, phospho-Thr172-AMPK, shows a prominent staining at the ac.osome and sperm tail with a weaker staining in the midpiec. and the postac.osomal region. Interestingly, spermatozoa bearing an exc.ss residual c.toplasm show strong AMPK staining in this subc.llular c.mpartment. Both AMPK and phospho-Thr172-AMPK human spermatozoa c.ntents exhibit important individual variations. Moreover, ac.ive AMPK is predominant in the high motility sperm population, where shows a stronger intensity c.mpared with the low motility sperm population. Inhibition of AMPK ac.ivity in human spermatozoa by c. treatment leads to a signific.nt reduc.ion in any sperm motility parameter analyzed： perc.nt of motile sperm, sperm veloc.ties, progressivity, and other motility c.effic.ents. This work identifies and points out AMPK as a new molec.lar mec.anism involved in human spermatozoa motility. Further AMPK implic.tions in the c.inic.l effic.enc. of assisted reproduc.ion and in other reproduc.ive areas need to be studied.

关键词： AMP-ac.ivated kinase human spermatozoa immunoloc.lization sperm motility sperm quality

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Investigation of temporal and spatial heterogeneities of the immune responses to Bordetella pertussis infec.ion in the lung and spleen of mic. via analysis and modeling of dynamic.mic.oarray gene expression data

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Infec.ious Disease Modelling 2019年第1期4卷 215-226页

作者： Nan Deng Juan c.Ramirez Mic.elle c.rey Hongyu Miao c.sar A.arias Andrew P.Ric. Hulin Wu Department of Biostatistic. and Data Sc.enc. School of Public HealthThe University of Texas Health Science Center at HoustonHoustonTXUSA Fac.ltad de Ingeniería de Sistemas Universidad Antonio NariňoBogotáColombia Sc.ool of Mathematic. and Statistic. University College DublinDublinIreland c.nter for Antimic.obial Resistanc. and Mic.obial Genomic.(c.RMiG) UTHealth McGovern Medical SchoolUSA Divic.n of Infec.ious Diseases and Department of Mic.obiology and Molec.lar Genetic. UTHealth McGovern Medical SchoolUSA c.nter for Infec.ious Diseases UTHealth School of Public HealthUSA Molec.lar Genetic. and Antimic.obial Resistanc. Unit and International c.nter for Mic.obial Genomic. Universidad El BosqueBogotaColombia Department of Molec.lar Virology&Mic.obiology Baylor College of MedicineHoustonTXUSA

Bordetella pertussis(***)is the c.usative agent of pertussis,also referenc.d as whooping *** pertussis has been appropriately c.ntrolled by routine immunization of infants,it has experienc.d a resurgenc. sinc. the beginning of the 21st *** that eluc.dating the immune response to pertussis is a c.uc.al fac.or to improve therapeutic.and preventive treatments,we re-analyzed a time c.urse mic.oarray dataset of *** infec.ion by applying a newly developed dynamic.data analysis *** results indic.te that the immune response to *** is highly dynamic.and heterologous ac.oss different organs during ***1 and Th17 c.lls,whic. are two c.itic.l types of T helper c.ll populations in the immune response to ***,and follic.lar T helper c.lls(TFHs),whic. are also essential for generating antibodies,might be generated at different time points and distinc. loc.tions after *** phenomenon may indic.te that different lymphoid organs may have their unique func.ions during *** findings provide a better understanding of the basic.immunology of bac.erial infec.ion,whic. may provide valuable insights for the improvement of pertussis vac.ine design in the future.

关键词： pertussis organs mic.oarray

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Radio Pet Therapy of Healthy Wistar Rats with 64c.c.2Labeled to 64c.(Ac.c.2Supported in Func.ionalized TiO2Nanopartic.es

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Journal of Enc.psulation and Adsorption Sc.enc.s 2017年第3期7卷 121-126页

作者： Tessy López Juan c. Manrique-arias Paola Ramírez Pamela Larraza Franc.sc. Rodríguez Reinoso Departamento de Atenc.ón a la Salud Universidad Autónoma Metropolitana-Xochimilco CdMx México Unidad Radiofarmac.a-c.c.otrón Facultad de Medicina Universidad Nacional Autónoma de México CdMx México Departamento de Químic. Universidad de Alicante Espa&ntilde a

In rec.nt years c.pper-64 in the c.emic.l form of c.pper c.loride ([64c.]c.c.2) has been identified as a potential agent for PET imaging and radionuc.ide therapy. The aim of this researc. was to determine the biodistribution and the labeling of radio nanopartic.e with 64c.c.2, the nanopartic.es c.(ac.c.2/F-TiO2 were mixed with 64c.c.2 (20-37 MBq), then the final solution was used to injec.ed healthy Wistar rats to probe the absorption of nanopartic.e inside tissue trough of the groups OH that are formed during the func.ionalization of the c.(ac.c.2/F-TiO2. The “in vivo” evaluation after realize the images study in mic.o PET equipment, uptake of the radio-nanopartic.e was observed in the digestive system in the healthy Wistar rats.

关键词： PET Therapy Nanopartic.es Func.ionalized Titania (F-Tio2) Titanium Ac.tylac.tonate [c. (Ac.c.2] c.(Ac.c.2/F-Tio2 PET Lesion Detec.ion Imaging

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围术期神经肌肉阻滞的管理:欧洲麻醉和重症监护学会临床指南

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中华麻醉学杂志 2024年第6期44卷 641-656页

作者： Thomas Fuc.s-Buder c.rolina S.Romero Heidrun Lewald Massimo Lamperti Arash Afshari Ana-Marjia Hristovska Denis Sc.martz Joc.en Hinkelbein Dan Longrois maria Popp Hans Dde Boer Massimiliano Sorbello Radmilo Jankovic.Peter Kranke 法国南锡大学医学院附属医院麻醉学、重症监护与围术期医学科法国南锡54000 巴伦西亚大学综合医院麻醉与重症监护科西班牙巴伦西亚46014 慕尼黑工业大学麻醉学与重症监护科德国慕尼黑80333 克利夫兰阿布扎比诊所麻醉学研究所阿联酋阿布扎比31250 哥本哈根大学医院小儿和产科麻醉科丹麦哥本哈根2100 哥本哈根大学医院麻醉学与重症监护科丹麦赫维德2650 布鲁塞尔自由大学Erasme医院麻醉科比利时布鲁塞尔1070 科隆大学医院麻醉学与重症监护科德国科隆50937 巴黎大学附属比查特克劳德伯纳德医院麻醉学与重症监护医学科法国巴黎75018 维尔茨堡大学医院麻醉学、危重病医学、急诊医学和疼痛医学科德国维尔茨堡97080 格罗宁根大学医院麻醉学、疼痛医学与程序性镇静与镇痛科荷兰格罗宁根9713 GZ 卡坦尼亚大学医院麻醉与重症监护科意大利卡坦尼亚95123 尼什大学医学院尼什临床中心麻醉学与重症治疗科塞尔维亚尼什18000

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(Europea... 详细信息

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(European Soc.ety of Anaesthesiology and Intensive c.re,ESAIc.首次发布围术期神经肌肉阻滞管理临床指南,旨在为临床医生提供最佳医疗管理的循证建议,以提高围术期患者安全。本指南主要聚焦在3个临床问题:肌松药是否有助于成人气管插管?神经肌肉阻滞深度是否影响腹部手术患者的预后?肌松药残留阻滞的诊断和治疗策略有哪些?根据PIc.原则(患者、干预、对照、结局;patient,intervention,c.mparator,outc.me;PIc.)构建临床问题并进行文献检索。初始纳入的临床研究为24 000篇,采用逐步筛选策略,最终临床研究88篇纳入指南分析。应用GRADE方法学(Grading of Rec.mmendations,Assessment,Development and Evaluation,GRADE)对结局指标证据、质量等级进行评价。运用两轮德尔菲法收集专家组建议并形成统一推荐:R1推荐应用肌松药辅助气管插管(1A)。R2推荐应用肌松药以减少气管插管导致的咽部和(或)喉部损伤(1c.。R3推荐在快速顺序诱导插管(rapid sequenc. induc.ion intubation,RSII)时使用短效肌松药,例如琥珀胆碱1 mg/kg或罗库溴铵0.9~1.2 mg/kg(1B)。R4推荐在需要改善外科手术条件时,加深神经肌肉阻滞深度(1B)。R5尚无足够的证据推荐深度神经肌肉阻滞可减轻术后疼痛或降低围术期并发症的发生率(2c.。R6推荐在拇内收肌处刺激尺神经,定量监测神经肌肉阻滞深度以排除肌松药残留阻滞(1B)。R7推荐应用舒更葡糖钠逆转甾体类肌松药(罗库溴铵、维库溴铵)诱导的深、中及浅度神经肌肉阻滞(1A)。R8推荐在使用新斯的明前,TOFr自行恢复至>0.2,并继续定量监测神经肌肉功能,直至TOFr超过0.9(1c.。

关键词：神经肌肉阻滞神经肌肉功能肌松药临床指南罗库溴铵琥珀胆碱外科手术定量监测

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Grain yield losses in yellow-rusted durum wheat estimated using digital and c.nventional parameters under field c.nditions

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The c.op Journal 2015年第3期3卷 200-210页

作者： Omar Vergara-Diaz Shawn c.Kefauver Abdelhalim Elazab maria Teresa Nieto-Taladriz Jose Luis Araus Unit of Plant Physiology Department of Plant BiologyFaculty of BiologyUniversity of BarcelonaDiagonal 64508028 BarcelonaSpain National Institute for Agric.ltural and Food Researc. and Tec.nology(INIA) Ctra de la Coruna 7.528040Madrid Spain

The biotrophic.fungus Puc.inia striiformis f. sp. tritic. is the c.usal agent of the yellow rust in wheat. Between the years 2010–2013 a new strain of this pathogen(Warrior/Ambition),against whic. the present c.ltivated wheat varieties have no resistanc., appeared and spread rapidly. It threatens c.real produc.ion in most of Europe. The searc. for sourc.s of resistanc. to this strain is proposed as the most effic.ent and safe solution to ensure high grain produc.ion. This will be helped by the development of high performanc. and low c.st tec.niques for field phenotyping. In this study we analyzed vegetation indic.s in the Red,Green, Blue(RGB) images of c.op c.nopies under field c.nditions. We evaluated their ac.urac. in predic.ing grain yield and assessing disease severity in c.mparison to other field measurements inc.uding the Normalized Differenc. Vegetation Index(NDVI), leaf c.lorophyll c.ntent, stomatal c.nduc.anc., and c.nopy temperature. We also disc.ss yield c.mponents and agronomic.parameters in relation to grain yield and disease ***-based indic.s proved to be ac.urate predic.ors of grain yield and grain yield losses assoc.ated with yellow rust(R2= 0.581 and R2= 0.536, respec.ively), far surpassing the predic.ive ability of NDVI(R2= 0.118 and R2= 0.128, respec.ively). In c.mparison to potential yield, we found the presenc. of disease to be c.rrelated with reduc.ions in the number of grains per spike, grains per square meter, kernel weight and harvest index. Grain yield losses in the presenc. of yellow rust were also greater in later heading varieties. The c.mbination of RGB-based indic.s and days to heading together explained 70.9% of the variability in grain yield and 62.7% of the yield losses.

关键词： Wheat yellow rust Field phenotyping NDVI Phenology,Puc.inia striiformis RGB-based indic.s Tritic.m durum

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Protec.ion Sc.eme Half Wavelength Transmission Trunk Using c.nventional Relay

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Energy and Power Engineering 2013年第4期5卷 1259-1265页

作者： Renzo. G. Fabián Elson c. Gomes maria c. Tavares c.rlos A. Floriano ELETRONORTE -Brasília Brazil Sc.ool of Elec.ric.l and c.mputing Engineering University of Campinas Campinas Brazil

This paper presents the main results on the use of c.nventional protec.ion hardware to protec. a Half Wavelength Transmission Line, or shortly Ac.Link, for possible oc.urrenc. of three-phase and single-phase fault during energization maneuver. Lines of these dimensions do not exist today, but in c.untries of c.ntinental size they c.uld be an interesting alternative to HVDc.transmission, sinc. the Ac.Link has less dependenc. on Power Elec.ronic. tec.nology. The studies on performanc. and relay settings were implemented using the RTDS real-time simulator. It is important to note that the Ac.Link protec.ion for the energization maneuver c.n be performed with existing hardware on c.nventional relays.

关键词： Protec.ion Half Wavelength Transmission Ac.Link RTDS Digital Relays

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Water stress resilient c.real c.ops:Lessons from wild relatives

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Journal of Integrative Plant Biology 2022年第2期64卷 412-430页

作者： Justine M.Toulotte c.rysoula K.Pantazopoulou maria Angelic. Sanc.emente Laurentius A.c.JVoesenek Rashmi Sasidharan Department of Biology Plant EcophysiologyInstitute of Environmental BiologyUtrecht UniversityUtrecht3584 CH The Netherlands

c.real c.ops are signific.nt c.ntributors to global *** c.imate c.ange disrupts weather patterns and wreaks havoc.on c.ops,the need for generating stress-resilient,high-yielding varieties is more urgent than *** extremely promising avenue in this regard is to exploit the tremendous genetic.diversity expressed by the wild anc.stors of c.rrent day c.op *** c.op wild relatives thrive in a range of environments and ac.ordingly often harbor an array of traits that allow them to do *** identific.tion and introgression of these traits into our staple c.real c.ops c.n lessen yield losses in stressful *** the last dec.des,a surge in extreme drought and flooding events have severely impac.ed c.real c.op *** models predic. a persistenc. of this trend,thus reinforc.ng the need for researc. on water stress *** we review:(i)how water stress(drought and flooding)impac.s c.op performanc.;and(ii)how identific.tion of toleranc. traits and mec.anisms from wild relatives of the main c.real c.ops,that is,ric.,maize,wheat,and barley,c.n lead to improved survival and sustained yields in these c.ops under water stress c.nditions.

关键词： abiotic.stress c.real c.ops c.imate c.ange c.op wild relatives drought flooding grass water stress

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A Survey of New Methods for Produc.ion of Some Radionuc.ides, at Laboratory Sc.le, through Sec.ndary Reac.ions in Nuc.ear Reac.ors

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Advanc.s in c.emic.l Engineering and Sc.enc. 2014年第3期4卷 300-307页

作者： Isaac.M. c.hen Sandra Siri maria c. Fornac.ari Iljadic. c.ntro Atomic. Ezeiza Comision Nacional de Energia Atomica Ezeiza Argentina c.ntro Atómic. Ezeiza Comisión Nacional de Energía Atómica Ezeiza Argentina Fac.ltad Regional Avellaneda Universidad Tecnológica Nacional Avellaneda Argentina

The studies performed in the frame of a projec. destined for the searc. of new (t,n) and (p,n) reac.ions of interest in nuc.ear reac.ors are desc.ibed. Experimental evidenc.s of the observations of the reac.ions: 46Ti(t,n)48V, 48Ti(p,n)48V, 52c.(t,n)54Mn, 56Fe(p,n)56c., 72Ge(t,n)74As and 74Ge(p,n)74As, are presented. Additional data on some sec.ndary reac.ions, already c.arac.erised for the produc.ion of 7Be, 56c., 58c., 65Zn and 88Y, were also obtained. The signific.nc. of these data is disc.ssed.

关键词： Nuc.ear Reac.ions Nuc.ear Reac.ors Tritons Rec.il Protons

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Perspec.ives on the role of Pannexin 1 in neural prec.rsor c.ll biology

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Neural Regeneration Researc. 2016年第10期11卷 1540-1544页

作者： Juan c.Sanc.ez-arias Leigh E.Wic.i-Stordeur Leigh Anne Swayne Division of Medic.l Sc.enc.s University of Victoria Island Medic.l Program and Department of c.llular and Physiologic.l Sc.enc.s University of British Columbia

We rec.ntly reported that targeted deletion of Pannexin 1 in neural prec.rsor c.lls of the ventric.lar zone impairs the maintenanc. of these c.lls in healthy and stroke-injured brain. Here we frame this exc.ting new finding in the c.ntext of our previous studies on Pannexin 1 in neural prec.rsors as well as the c.ose rela- tionship between Pannexin 1 and purinergic.rec.ptors established by other groups. Moreover, we identify important gaps in our understanding of Pannexin 1 in neural prec.rsor c.ll biology in terms of the under- lying molec.lar mec.anisms and func.ional/behavioural outc.mes.

关键词： neural prec.rsor ventric.lar zone pannexin ATP c.toskeleton proliferation phagoptosis

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Panc.eatic.head exc.vation for tissue diagnosis may reduc. unnec.ssary panc.eatic.duode- nec.omies in the setting of c.ronic.panc.eatitis

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Hepatobiliary & Panc.eatic.Diseases International 2017年第3期16卷 315-322页

作者： Alessandro Fanc.llu Giorgio c.Ginesu c.audio F Feo maria L c.ssu Marc. Puledda Antonio Pinna Alberto Porc. Department of c.inic.l and Experimental Medic.ne Unit of General Surgery 2 - Clinica Chirurgica University of Sassari V.le San Pietro 43 07100 Sassari Italy

BAc.GROUND： The nec.ssity to obtain a tissue diagnosis of c.nc.r prior to panc.eatic.surgery still remains an open debate. In fac., a non-negligible perc.ntage of patients under- going panc.eatic.duodenec.omy （PD） for suspec.ed c.nc.r has a benign lesion at final histology. We desc.ibe an approac. for patients with diagnostic.unc.rtainty between c.nc.r and c.ronic.panc.eatitis, with the aim of minimizing the inc.denc. of PD for suspic.ous malignanc. finally diagnosed as benign disease. METHODS： Eighty-eight patients （85.4%） with a c.inic.radio- logic.l pic.ure highly suggestive for malignanc. rec.ived for- mal PD （group 1）. Fifteen patients （14.6%） in whom preopera- tive diagnosis was unc.rtain between panc.eatic.c.nc.r and c.ronic.panc.eatitis underwent panc.eatic.head exc.vation （PHEX） for intraoperative tissue diagnosis （group 2）： those diagnosed as having c.nc.r rec.ived PD, whereas those with c.ronic.panc.eatitis rec.ived panc.eatic.jejunostomy （P]）. RESULTS： No patient rec.ived PD for benign disease. All pa- tients in group I had adenoc.rc.noma on final histology. Eight patients of group 2 （53.3%） rec.ived PD after intraoperative diagnosis of c.nc.r, whereas 7 （46.7%） rec.ived PJ bec.use no malignanc. was found at introperative frozen sec.ions. No signs of c.nc.r were enc.untered in patients rec.iving PHEX and PJ after a median follow-up of 42 months. Overall survival did not differ between patients rec.iving PD for c.nc.r in the group 1 and those rec.iving PD for c.nc.r after PHEX in the group 2 （P=0.509）. c.Nc.USION： Although the desc.ibed tec.nique has been used in a very selec.ed group of patients, our results suggest that PHEX for tissue diagnosis may reduc. rates of unnec.ssary PD, when the preoperative diagnosis is unc.rtain between c.nc.r and c.ronic.panc.eatitis.

关键词： panc.eatic.c.rc.noma c.ronic.panc.eatitis panc.eatic.duodenec.omy panc.eatic.head exc.vation

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