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Role of sex hormones in gastrointestinal motility in pregnant and non-pregnant rats

World Journal of Gastroenterology 2016年第25期22卷 5761-5768页

作者： Juliana Fernandes Matos Madileine Francely Americo Yuri Karen Sinzato Gustavo Tadeu Volpato Luciana Aparecida Corá marcos Felipe Freitas Calabresi Ricardo Brandt oliveira debora Cristina Damasceno Jose Ricardo Arruda Miranda Sao Paulo State University BotucatuSP 18618-970Brazil Instituto de Ciencias Biologicas e da Saude Federal University of Mato GrossoBarra do GarcasMT 78600-000Brazil Universidade Estadual de Ciencias da Saude de Alagoas MaceioAL 57010-300Brazil Sao Paulo University Ribeirao PretoSP 14049-900Brazil

AIM: To correlate gastric contractility, gastrointestinal transit, and hormone levels in non-pregnant(estrous cycle) and pregnant rats using noninvasive techniques. METHODS: Female rats(n = 23) were randomly divided into(1) non-pregnant,(contractility, n =6; transit, n = 6); and(2) pregnant(contractility, n = 5; transit, n = 6). In each estrous cycle phase or at 0, 7, 14, and 20 d after the confirmation of pregnancy, gastrointestinal transit was recorded by AC biosusceptometry(ACB), and gastric contractility was recorded by ACB and electromyography. After each recording, blood samples were obtained for progesterone and estradiol determination. RESULTS: In the estrous cycle, despite fluctuations of sex hormone levels, no significant changes in gastrointestinal motility were observed. Days 7 and 14 of pregnancy were characterized by significant changes in the frequency of contractions(3.90 ± 0.42 cpm and 3.60 ± 0.36 cpm vs 4.33 ± 0.25 cpm) and gastric emptying(168 ± 17 min and 165 ± 15 min vs 113 ± 15 min) compared with day 0. On these same days, progesterone levels significantly increased compared with control(54.23 ± 15.14 ng/m L and 129.96 ± 30.52 ng/mL vs 13.25 ± 6.31 ng/mL). On day 14, we observed the highest level of progesterone and the lowest level of estradiol compared with day 0(44.3 ± 15.18 pg/mL vs 24.96 ± 5.96 pg/mL). CONCLUSION: Gastrointestinal motility was unaffected by the estrous cycle. In our data, high progesterone and low estradiol levels can be associated with decreased contraction frequency and slow gastric emptying.

关键词： Reproductive physiological process Sex hormones Gastrointestinal motility Magnetic fields Gastric emptying

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Characterization of Clinical and Environmental Isolates of <i>Cryptococcus neoformans</i>/<i>Cryptococcus gattii</i>Complex Maintained in Yeast Culture Collection in São Paulo, Brazil

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Open Journal of Epidemiology 2018年第2期8卷 76-92页

作者： Pedro Henrique Magalh&atilde es Cardoso Bosco Christiano Maciel da Silva Jairo Ivo dos Santos Rennan Luiz oliveira dos Santos Diniz Pereira Leite Jú nior marcos Ereno Auler Luciana da Silva Ruiz Eriques Gonç alves da Silva Dé bora Moreira Carina Domaneschi Francisco de Assis Baroni Marcia de Souza Carvalho Melhem Marilena dos Anjos Martins Claudete Rodrigues Paula Faculty of Veterinary Medicine and Zootechny-FMVZ-USP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Faculty of Medicine/FMUSP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Federal University of Santa Catarina (UFSC) Florian&oacute polis Brazil Faculty of dentistry University of S&#227 o Paulo (FOUSP) S&#227 o Paulo Brazil Faculty of Medicine Federal University of Mato Grosso (UFMT) Cuiab&aacute Brazil Faculty of Pharmacy University of the Western Center of Paran&aacute (UNICENTRO) Guarapuava Brazil Institute Adolfo Lutz (IAL) Bauru Brazil Federal Rural University of Rio de Janeiro (UFRRJ) Serop&eacute dica Brazil Institute Adolfo Lutz (IAL) S&#227 o Paulo Brazil

Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.

关键词： Cryptococcus Complex Genotyping Protease Phospholipase Etest Culture Collection

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Geo-environmental parametric 3D models of SARS-CoV-2 virus circulation in hospital ventilation systems

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Geoscience Frontiers 2022年第6期13卷 244-256页

作者： Carla Gabriela Carlot Zorzi Alcindo Neckel Laércio Stolfo Maculan Grace Tibério Cardoso Leila Dal Moro Alexandre Almeida del Savio Leopoldo D.Z.Carrasco marcos L.S.oliveira Eliane Thaines Bodah Brian William Bodah Faculdade Meridional IMED304-Passo Fundo-RS 99070-220Brazil Universidad de Lima Avenida Javier Prado Este 4600–Santiago de Surco 1503Peru department of Civil and Environmental.Universidad de la Costa CUCCalle 58#55–66BarranquillaAtlánticoColombia State University of New York Onondaga Community College4585 West Seneca TurnpikeSyracuseNY 13215USA Thaines and Bodah Center for Education and development 840 South Meadowlark LaneOthelloWA 99344USA

The novel coronavirus, SARS-CoV-2, has the potential to cause natural ventilation systems in hospital environments to be rendered inadequate, not only for workers but also for people who transit through these environments even for a limited duration. Studies in of the fields of geosciences and engineering,when combined with appropriate technologies, allow for the possibility of reducing the impacts of the SARS-CoV-2 virus in the environment, including those of hospitals which are critical centers for healthcare. In this work, we build parametric 3D models to assess the possible circulation of the SARS-CoV-2 virus in the natural ventilation system of a hospital built to care infected patients during the COVID-19 pandemic. Building Information Modeling(BIM) was performed, generating 3D models of hospital environments utilizing Revit software for Autodesk CFD 2021. The evaluation considered dimensional analyses of 0°, 45°, 90° and 180°. The analysis of natural ventilation patterns on both internal and external surfaces and the distribution of windows in relation to the displacement dynamics of the SARS-CoV-2 virus through the air were considered. The results showed that in the external area of the hospital, the wind speed reached velocities up to 2.1 m/s when entering the building through open windows. In contact with the furniture, this value decreased to 0.78 m/s. In some internal isolation wards that house patients with COVID-19, areas that should be equipped with negative room pressure, air velocity was null. Our study provides insights into the possibility of SARS-CoV-2 contamination in internal hospital environments as well as external areas surrounding hospitals, both of which encounter high pedestrian traffic in cities worldwide.

关键词： COVID-19 global epidemic Dimensional analysis Wind velocity Hospital environment Contamination

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Common bean SNP alleles and candidate genes affecting photosynthesis under contrasting water regimes

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Horticulture Research 2021年第1期8卷 110-123页

作者： Susana Trindade Leitao Maria Catarina Bicho Priscila Pereira Maria Joao Paulo marcos Malosetti Susana de Sousa Araujo Fred van Eeuwijk Maria Carlota Vaz Patto Instituto de Tecnologia Quimica e Biológica Antonio Xavier Universidade Nova de LisboaOeirasPortugal Wageningen University&Research WageningenThe Netherlands Present address:Instituto Superior de Agronomia Universidade de LisboaLisboaPortugal Present address:Nunhems Vegetable Seeds NunhemThe Netherlands Present address:Association BLC3-Technology and Innovation Campus Centre Bio R&D UnitOliveira do HospitalLisboaPortugal

Water deficit is a major worldwide constraint to common bean(Phaseolus vulgaris L.)production,being photosynthesis one of the most affected physiological *** gain insights into the genetic basis of the photosynthetic response of common bean under water-limited conditions,a collection of 158 Portuguese accessions was grown under both well-watered and water-deficit *** gas-exchange parameters were measured and photosynthetic pigments *** same collection was genotyped using SNP arrays,and SNP-trait associations tested considering a linear mixed model accounting for the genetic relatedness among accessions.A total of 133 SNP-trait associations were identified for net CO 2 assimilation rate,transpiration rate,stomatal conductance,and chlorophylls a and b,carotenes,and xanthophyll *** of these associations were detected under water-deficit and 43 under well-watered conditions,with only two associations common to both *** candidate genes revealed that stomatal regulation,protein translocation across membranes,redox mechanisms,hormone,and osmotic stress signaling were the most relevant processes involved in common bean response to water-limited *** candidates are now preferential targets for common bean water-deficit-tolerance ***,new sources of water-deficit tolerance of Andean,Mesoamerican,and admixed origin were detected as accessions valuable for breeding,and not yet explored.

关键词： breeding water assimilation

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A safe haven of SARS-CoV-2 in the environment:Prevalence and potential transmission risks in the effluent,sludge,and biosolids

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Geoscience Frontiers 2022年第6期13卷 302-314页

作者： Bashir Adelodun Pankaj Kumar Golden Odey Fidelis Odedishemi Ajibade Rahmat Gbemisola Ibrahim Saad A.M.Alamri Sulaiman A.Alrumman Ebrahem M.Eid Vinod Kumar Khalid Adeola Adeyemi Ashish Kumar Arya Archana Bachheti marcos L.S.oliveira Kyung Sook Choi department of Agricultural Civil Engineering Kyungpook National UniversityDaegu 41566South Korea department of Agricultural and Biosystems Engineering University of IlorinPMB 1515Ilorin 240003Nigeria Agro-ecology and Pollution Research Laboratory Department of Zoology and Environmental ScienceGurukula Kangri(Deemed to be University)Haridwar 249404UttarakhandIndia department of Civil and Environmental Engineering Federal University of TechnologyPMB 704AkureNigeria Key Laboratory of Environmental Biotechnology Research Centre for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085PR China University of Chinese Academy of Sciences Beijing 100049PR China Kwara State Ministry of Health IlorinKwara StateNigeria Biology department College of ScienceKing Khalid UniversityAbha 61321Saudi Arabia Botany department Faculty of ScienceKafrelsheikh UniversityKafr El-Sheikh 33516Egypt department of Environmental Science Graphic Era(Deemed to be University)Deharadun248002 UttarakhandIndia department of Civil and Environmental Universidad De La CostaCalle 58#55-66080002 BarranquillaAtlánticoColombia Institute of Agricultural Science&Technology KyungpookNational UniversityDaegu 41566South Korea

The novel coronavirus,SARS-CoV-2,which has caused millions of death globally is recognized to be unstable and recalcitrant in the environment,especially in the way it has been evolving to form new and highly transmissible *** particular concerns are human-environment interactions and the handling and reusing the environmental materials,such as effluents,sludge,or biosolids laden with the SARS-CoV-2 without adequate treatments,thereby suggesting potential transmission and health *** study assesses the prevalence of SARS-CoV-2 RNA in effluents,sludge,and ***,we evaluate the environmental,ecological,and health risks of reusing these environmental materials by wastewater/sludge workers and farmers.A systematic review of literature from the Scopus database resulted in a total of 21 articles(11 for effluents,8 for sludge,and 2 for biosolids)that met the criteria for meta-analysis,which are then subdivided into 30 meta-analyzed *** prevalence of SAR-CoV-2 RNA in effluent and sludge based on random-effect models are 27.51 and 1012.25,respectively,with a 95%CI between 6.14 and 48.89 for the effluent,and 104.78 and 1019.71 for the ***,the prevalence of SARS-CoV-2 RNA in the biosolids based on the fixed-effect model is 30.59,with a 95%CI between 10.10 and *** prevalence of SARS-CoV-2 RNA in environmental materials indicates the inefficiency in some of the treatment systems currently deployed to inactivate and remove the novel virus,which could be a potential health risk concern to vulnerable wastewater workers in particular,and the environmental and ecological issues for the population at *** timely review portends the associated risks in handling and reusing environmental materials without proper and adequate treatments.

关键词： SARS-CoV-2 COVID-19 Environmental materials Health risks Wastewater workers

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Atherogenic Indices and HDL Particle Size as Laboratory Parameters to Evaluate Cardiovascular Risk in the Presence of Dyslipidemia

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Journal of Biophysical Chemistry 2014年第2期5卷 24-32页

作者： Ana Paula C.Santos marcos S.Vieira debora F.deus Rogerio Jorge Bde oliveira Aleksandra T.Morikawa Roque Aras Ajax M.Atta Fabio D.Couto Raul C.Maranhao Ricardo D.Couto Clinical Biochemistry Laboratory Department of Clinical and Toxicological AnalysisFaculty of PharmacyFederal University of Bahia/UFBASalvadorBrazil Naval Hospital of Salvador Brazilian Marine ForcesSalvadorBrazil Lipid Metabolism Laboratory of the Heart Institute(InCor-Clinical Hospital of Medical School) Faculty of PharmacyUniversity of Sao Paulo/USPSao PauloBrazil Ana Neri Hospital SESAB and Federal University of Bahia/UFBA Faculty of MedicineSalvadorBrazil Laboratory of Research in Immunology(LAPIM) Department of Clinical and Toxicological AnalysisFaculty of PharmacyFederal University of Bahia/UFBASalvadorBrazil Laboratory of Molecular Biology and Genetics at Center of Agricultural Environmental and Biological Sciences(CCAAB-UFRB)Cruz das AlmasBrazil Faculty of Pharmaceutical Sciences Clinical Chemistry DepartmentUniversity of Sao Paulo/USPSao PauloBrazil Central Clinical Laboratory of Professor Edgar Santos University Hospital Federal University of Bahia/UFBASalvadorBrazil

Dyslipidemia may influence enzymes and transfer proteins needed to the lipoprotein particle remodeling. Calculated indices and evaluation of lipoprotein particle size have widely been used to predict cardiovascular risk. The aim of this study was to evaluate HDL particle size and LDL particle size estimate based on TG/HDL-C as well as apoB/apoA-I ratio as possible marker and atherogenic indices, respectively, of cardiovascular disease risk in the presence of dyslipidemia. We evaluated 100 individuals of both gender, without treatment with lipid-lowering drugs, 27 normolipidemic and 73 dyslipidemic, such as isolated hypercholesterolemia (n = 16), isolated hypertriglyceridemia (n = 17), low HDL-C (n = 26) and mixed dyslipidemia (n = 14). The HDL particle size did not differ between groups. The TG/HDL-C ratio was higher in groups with isolated hypertriglyceridemia (4.2 ± 1.5), low HDL-C (5.2 ± 3.1) and mixed dyslipidemia (5.3 ± 1.6). The apoB/apoA-I ratio was increased in all groups of dyslipidemia (apoB/apoA-I > 0.5) when compared to normolipidemic (apoB/apoA-I = 0.5, p deling markers and the use of calculated indices may contribute to the evaluation of cardiovascular disease risk when dyslipidemia take place.

关键词： Dyslipidemia Atherogenic Indices Cardiovascular Risk

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Use of geospatial tools to predict the risk of contamination by SARS-CoV-2 in urban cemeteries

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Geoscience Frontiers 2022年第6期13卷 331-346页

作者： Paloma Carollo Toscan Alcindo Neckel Laércio Stolfo Maculan Cleiton Korcelski marcos L.Soliveira Eliane Thaines Bodah Brian William Bodah Henrique Aniceto Kujawa Affonso Celso Gonalves Faculdade Meridional IMED304Passo FundoRS 99070-220Brazil department of Civil and Environmental Universidad de la CostaCUCCalle 58#55–66BarranquillaAtlánticoColombia Universidad de Lima Departamento de Ingeniería civil y ArquitecturaAvenida Javier Prado Este 4600Santiago de Surco 1503Peru State University of New York Onondaga Community College4585 West SenecaTurnpikeSyracuseNY 13215USA Thaines and Bodah Center for Education and development 840 South Meadowlark LaneOthelloWA 99344USA University of Perugia Piazza Università106123 PerugiaItaly State University of Western Paraná–UNIOESTE Center of Agrarian SciencesRua Pernambuco1777CentroMarechal Cândido RondonPR 85960-000Brazil

Urban cemeteries are increasingly surrounded by areas of high residential density as urbanization continues world-wide. With increasing rates of mortality caused by the novel coronavirus, SARS-CoV-2,urban vertical cemeteries are experiencing interments at an unprecedented rate. Corpses interred in the 3rd to 5th layer of vertical urban cemeteries have the potential to contaminate large adjacent *** general objective of this manuscript is to analyze the reflectance of altimetry, normalized difference vegetation index(NDVI) and land surface temperature(LST) in the urban cemeteries and neighbouring areas of the City of Passo Fundo, Rio Grande do Sul, Brazil. It is assumed that the population residing in the vicinity of these cemeteries may be exposed to SARS-CoV-2 contamination through the displacement of microparticles carried by the wind as a corpse is placed in the burial niche or during the first several days of subsequent fluid and gas release through the process of decomposition. The reflectance analyses were performed utilizing Landsat 8 satellite images applied to altimetry, NDVI and LST, for hypothetical examination of possible displacement, transport and subsequent deposition of the SARS-CoV-2 virus. The results showed that two cemeteries within the city, cemeteries A and B could potentially transport SARS-CoV-2 of nanometric structure to neighboring residential areas through wind *** two cemeteries are located at high relative altitudes in more densely populated regions of the *** NDVI, which has been shown to control the proliferation of contaminants, proved to be insufficient in these areas, contributing to high LST values. Based on the results of this study, the formation and implementation of public policies that monitor urban cemeteries is suggested in areas that utilize vertical urban cemeteries in order to reduce the further spread of the SARS-CoV-2 virus.

关键词： Remote sensing Reflectance temperature Atmospheric contamination Urban environment SARS-CoV-2

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Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni

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World Journal of Gastroenterology 2008年第38期14卷 5842-5850页

作者： Sheilla Andrade oliveira Bruno Solano Freitas Souza Carla Adriana Guimares-Ferreira Elton Sá Barreto Siane Campos Souza Luiz Antonio Rodrigues Freitas Ricardo Ribeiro-dos-Santos Milena Botelho Pereira Soares Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Centro de Pesquisas Aggeu Magalhes Fundao Oswaldo Cruz Recife Av Moraes Rego s/n Cidade Universitária Recife 50670-420 PE Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas Salvador 40295-001 BA Brazil Centro de Pesquisas Gonalo Moniz Fundao Oswaldo Cruz Rua Waldemar Falco 121 Brotas 40295-001 Salvador BA Brazil Hospital So Rafael Av So Rafael 2152 So Marcos Salvador 41256-900 BA Brazil

AIM： To investigate the potential of bone marrow mononuclear cells （BM-MCs） in the regeneration of hepatic lesions induced by Schistosoma mansoni （***） chronic infection. METHODS： Female mice chronically infected with *** were treated with BM-MCs obtained from male green fluorescent protein （GFP） transgenic mice by intravenous or intralobular injections. Control mice received injections of saline in similar conditions, Enzyme-linked immunosorbent assay （ELISA） assay for transforming growth factor-beta （TGF-β）, polymerase chain reaction （PCR） for GFP DNA, immunofluorescence and morphometric studies were performed. RESULTS： Transplanted GFP^＋ cells migrated to granuloma areas and reduced the percentage of liver fibrosis. The presence of donor-derived cells was confirmed by Fluorescence in situ hybridization （FISH） analysis for detection of cells bearing Y chromosome and by PCR analysis for detection of GFP DNA. The levels of TGF-β, a cytokine associated with fibrosis deposition, in liver fragments of mice submitted to therapy were reduced. The number of oval cells in liver sections of ***-infected mice increased 3-4 fold after transplantation. A partial recovery in albumin expression, which is decreased upon infection with ***, was found in livers of infected mice after cellular therapy. CONCLUSION： In conclusion, transplanted BMCs migrate to and reduce the damage of chronic fibrotic liver lesions caused by ***.

关键词： Schistosoma mansoni Liver injury Fibrosis Bone marrow cells Transplantation Albumin Oval cells

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Civic and Social Applications to Support Public Policies on Health

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Management Studies 2019年第6期7卷 543-551页

作者： Eduardo Amadeu Dutra Moresi Michel Carmo Lopes Júlio Cezar Alves dos Santos marcos Augusto Alves Tito de Morais Mário de oliveira Braga Filho Jair Alves Barbosa Catholic University of Brasília DFBrazil

The discussion with emphasis on social control is expressed in new guidelines for its effectiveness through normative instruments and the legal creation of institutional spaces that guarantee the participation of organized civil society in the direct supervision of the executive in the three spheres of *** participation for the strengthening of public health in Brazil has undergone complex processes of change,which have resulted in an increasingly qualified,deliberative,independent,and representative social control ***,policy makers need to identify new technologies and carefully analyze their potential before they begin to exert their breaking power in the economy and *** and unlimited connectivity deserve special attention from the public power for the ability to achieve the digital inclusion of the citizen through *** requires a transformation of the governance structure into a platform that is based on the tripod transparency,participation,and collaboration,which relies on the Internet,social networks and connectivity.

关键词： social participation public health policies mobile devices civic social applications

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Ingestive Behavior and Nutritional and Physiological Parameters of Sheep Fed Diets Based on Cashew Byproduct

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Veterinary Science Research 2019年第2期1卷 25-35页

作者： Clésio dos Santos Costa Tallita da Ponte Ribeiro roberto Cláudio Fernandes Franco Pompeu Fernando Lisboa Guedes Marco Aurélio delmondes Bomfim Arnaud Azevedo Alves Luciana Freitas Guedes delano de Sousa oliveira marcos Cláudio Pinheiro Rogério department of Animal Science Federal University of CearáFortalezaCearáBrazil department of Animal Science State University Vale do AcaraúSobralCearáBrazil Brazilian Agricultural Research Corporation Embrapa Goats&SheepEstrada Sobral-GroaírasKm 0462.011-970SobralCearáBrazil department of Animal Science Federal University of PiauíTeresinaPiauíBrazil

Objective of this study was to evaluate the ingestive behavior,feed efficiency,and nutritional and physiological parameters of sheep that were fed diets based on byproducts from the processing of *** experiment was conducted in a completely randomized design with a 4×2 factorial arrangement with four levels of inclusion(6%,11%,16%,and 21%of cashew byproduct)and two forms of processing—with chemical treatment(CT)and without chemical treatment(NCT).The interaction levels of inclusion of the byproduct of cashew versus chemical treatments was not(P>0.05)for the dry matter intake,consumption of organic *** effect was observed(P>0.05)for the intake of dry matter in function of the type of chemical treatment used in the byproduct of *** was no effect of interaction(Pde protein(P>0.05).The inclusion of the byproduct of cashew did not influence the behavioral parameters,intake and digestibility of the diets of sheep,being recommended to use up to the level of 21%.

关键词： Ammoniation Anacardium occidentale L Agroindustrial byproducts Feeding Time Rumination

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