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抗生素亚胺培南探针分子设计及传感性能研究

有色金属材料与工程 2024年第2期45卷 55-61页

作者：朱彦西陈爱英毛燕飞上海理工大学材料与化学学院上海200093 上海交通大学医学院附属新华医院麻醉与重症医学科上海200092

抗生素亚胺培南(imipenem,IPM)用药治疗败血症患者感染需要快速做出反应,目前的血药监测方法严重延迟药物注射。设计金属元素掺杂型石墨烯量子点(graphene quantum dots,GQDs),可实现荧光法快速测定IPM和开辟荧光法检测抗生素的新途径... 详细信息

抗生素亚胺培南(imipenem,IPM)用药治疗败血症患者感染需要快速做出反应,目前的血药监测方法严重延迟药物注射。设计金属元素掺杂型石墨烯量子点(graphene quantum dots,GQDs),可实现荧光法快速测定IPM和开辟荧光法检测抗生素的新途径。采用水热法制备了铁掺杂GQDs(Fe-GQDs),用于快速探测IPM。采用透射电子显微镜(transmission electron microscopy,TEM)、X射线光电子能谱(X-ray photoelectron spectroscopy,XPS)以及荧光光谱等方法,探讨不同铁源起始剂对GQDs荧光性能及传感性能的影响,并分析了乙酸铁为铁源制备的Fe-GQDs的微观结构及荧光性能。结果表明:以乙酸铁为起始剂制备的Fe-GQDs荧光性能最佳,且对IPM具有显著的荧光淬灭效应。所制备的Fe-GQDs荧光淬灭强度(F/F_(0))与IPM浓度(concentration,C)呈线性关系,线性方程为F/F_(0)=0.983-4.23×10^(-3)C,线性相关系数(R 2)为0.997,线性范围为0.007~0.073 g/L。Fe-GQDs探针分子对血浆中常见有机分子和金属离子具有良好的抗干扰性,检测IPM误差为1.6%~4.6%。

关键词：石墨烯量子点掺杂抗生素荧光性能传感特性

245 GHz subharmonic receiver with on-chip antenna for gas spectroscopy application

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Journal of Semiconductors 2018年第12期39卷 160-165页

作者： yanfei mao Shiju E Klaus Schmalz J.Christoph Scheytt Engineering College Zhejiang Normal University IHP Microelectronics Heinz Nixdorf Institute Paderborn University

A 2 nd transconductance subharmonic receiver for 245 GHz spectroscopy sensor applications has been proposed. The receiver consists of a 245 GHz on-chip folded dipole antenna, a CB（common base） LNA, a 2 nd transconductance SHM（subharmonic mixer）, and a 120 GHz push-push VCO with 1/64 divider. The receiver is fabricated in f;/f;= 300/500 GHz SiGe:C BiCMOS technology. The receiver dissipates a low power of 288 *** with the on-chip antenna, the receiver is measured on-chip with a conversion gain of 15 dB, a bandwidth of 15 GHz, and the chip will be utilized in PCB board design for gas spectroscopy sensor application.

关键词： SiGe BiCMOS CB LNA 2nd transconductance subharmonic receiver 245 GHz on-chip antenna gas spectroscopy

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

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Molecular Plant 2017年第7期10卷 1011-1013页

作者： Mugui Wang yanfei mao Yuming Lu Xiaoping Tao Jian-kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence inMolecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Dear Editor, The class 2/type Ⅱ clustered regularly interspaced short palindromic repeat （CRISPR）/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs （sgRNAs） to achieve multiplex gene editing in plant cells.

关键词： TARGETED MUTAGENESIS CPF1

Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems

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Journal of Integrative Plant Biology 2018年第8期60卷 626-631页

作者： Mugui Wang yanfei mao Yuming Lu Zhidan Wang Xiaoping Tao Jian-Kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.

关键词： Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems PAM Figure LEA

A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana

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Science Bulletin 2015年第15期60卷 1332-1347页

作者： Wenshan Liu Xiaohong Zhu Mingguang Lei Qingyou Xia Jose Ramon Botella Jian-Kang Zhu yanfei mao School of Life Sciences Chongqing University Shanghai Center for Plant Stress Biology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences State Key Laboratory of Silkworm Genome Biology Southwest University Department of Horticulture and Landscape Architecture Purdue University School of Agriculture and Food Sciences University of Queensland

The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations andcorrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA(sg RNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sg RNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications.

关键词： CRISPR/Cas9 Targeted gene editing Genome engineering Arabidopsis thaliana

Application of the CRISPR-Cas System for Efficient Genome Engineering in Plants

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Molecular Plant 2013年第6期6卷 2008-2011页

作者： mao, yanfei Zhang, Hui Xu, Nanfei Zhang, Botao Gou, Feng Zhu, Jian-Kang Chinese Acad Sci Shanghai Ctr Plant Stress Biol Shanghai 200032 Peoples R China Univ Chinese Acad Sci CAS Shanghai 200032 Peoples R China Purdue Univ Dept Hort & Landscape Architecture W Lafayette IN 47907 USA

Dear Editor, Recently, engineered endonucleases, such as Zinc-Finger Nucleases （ZFNs）（Carroll, 2011）, Transcription Activator-Like Effector Nucleases （TALENs）（Mahfouz et al., 2011; Li et al., 2012）, and Clustered Regularly Interspaced Short Palindromic Repeats （CRISPR）-associated （Cas） systems （Cong et al., 2013） have been successfully used for gene editing in a variety of species. These systems generate double-strand breaks （DSBs） at target loci to drive site-specific DNA sequence modifica- tions. The modifications include sequence insertion and deletion and other mutations in the host genomes via the error-prone non-homologous end joining （NHEJ） pathway or sequence correction or replacement through the error-free homologous recombination （HR） pathway （Symington and Gautier, 2011）. Here, we show that the CRISPR-Cas system can be applied to generate targeted gene mutations and gene corrections in plants, and the system can also be readily engi- neered to achieve deletion of large DNA fragments and for multiplex gene editing in plants.

关键词： CAS系统基因组工程植物基因应用 DNA双链断裂重复序列同源重组核酸内切酶

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

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Molecular Plant 2017年第7期10卷 1007-1010页

作者： Mugui Wang Yuming Lu Jose Ramon Botella yanfei mao Kai Hua Jian-kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Plant Genetic Engineering Laboratory School of Agriculture and Food Sciences University of Queensland Brisbane QLD 4072 Austraria Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Dear Editor, Rice （Oryza sativa） is the staple food for more than half of the world＇s population. Technologies enabling precise and efficient DNA knock-in or replacement, hereinafter referred to as KI, have the pot... 详细信息

关键词： DNA REPLICONS GENOME

基于万古霉素高效还原Ag纳米粒子体系的分光光度法检测

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有色金属材料与工程 2024年第4期45卷 41-50页

作者：彭申周柳艳陈爱英毛燕飞任荣荣上海理工大学材料与化学学院上海200093 上海交通大学医学院附属新华医院麻醉外科重症监护室上海200092

万古霉素(vancomycin,Van)是临床实践中常用的抗生素,其用于预防传染病,但在环境水微量污染物检测中常常被忽略。利用溶剂热反应过程中Ag+选择性氧化Van中酚类羟基团特性,开发了一种利于分光光度法检测废水中Van的方法。探讨了反应温度... 详细信息

万古霉素(vancomycin,Van)是临床实践中常用的抗生素,其用于预防传染病,但在环境水微量污染物检测中常常被忽略。利用溶剂热反应过程中Ag+选择性氧化Van中酚类羟基团特性,开发了一种利于分光光度法检测废水中Van的方法。探讨了反应温度和时间等对氧化还原反应的影响,建立了Van质量浓度(concentration,C)与Ag纳米粒子溶液吸光度(absorbance,A)之间的线性关系。发现C与A呈双线性关系:在线性浓度范围为1~15μg/mL时,线性关系为A_(1)=0:0017+0:012C_(1),检测限为0.5μg/mL;在20~40μg/mL时,A_(2)=0:11+0:0055C_(2)。该方法成功应用于废水中Van检测,回收率为96.0%~104.5%,废水中可能存在其他物质或颗粒,干扰了Van的测量,导致回收率计算结果超过100%。

关键词：万古霉素银纳米粒子光谱法检测

基于治疗前多维度参数的列线图预测小细胞肺癌患者铂类化疗的总生存期

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温州医科大学学报 2024年第7期54卷 589-597页

作者：叶若雷苏燕萍毛艳菲张轶虹张莹徐艳艳骆松梅浙江中医药大学药学院浙江杭州310000 温州医科大学附属第五医院药学部浙江丽水323000 温州医科大学附属第五医院放射科浙江丽水323000 温州医科大学附属第五医院浙江省影像诊断与介入微创研究重点实验室浙江丽水323000

目的:探讨基于治疗前多维度参数构建的列线图在预测小细胞肺癌(SCLC)患者铂类化疗总生存期(OS)的应用价值。方法:回顾性分析温州医科大学附属第五医院2014年2月至2023年2月经病理证实为SCLC,且一线治疗方式为规律铂类化疗的患者155例,... 详细信息

目的:探讨基于治疗前多维度参数构建的列线图在预测小细胞肺癌(SCLC)患者铂类化疗总生存期(OS)的应用价值。方法:回顾性分析温州医科大学附属第五医院2014年2月至2023年2月经病理证实为SCLC,且一线治疗方式为规律铂类化疗的患者155例,对入组患者进行电话随访以获取OS。采用7:3比例将所有患者随机分为训练组(n=111)和验证组(n=44)。所有患者治疗前均接受胸部CT平扫及各项血液学检查。采用最小绝对收缩与选择算子(LASSO)回归对各项因素进行特征筛选,而后经单因素及多因素Cox回归分析筛选预测SCLC患者铂类化疗OS的独立预测因素,绘制列线图。绘制时间依赖性受试者工作特征(TimeROC)曲线、时间依赖曲线下面积(TimeAUC)图、校准曲线及决策曲线分析(DCA)评估模型效能和临床价值,Kaplan-Meier曲线分析高、低风险组对患者预后的影响。结果:训练组中,LASSO回归初筛后共得到5个与SCLC铂类化疗预后相关的因素,分别为性别、美国退伍军人肺癌研究组(VALSG)分期、铂敏感性、细胞角蛋白十九片段(Cyfra21-1)、血小板,以上特征经单因素及多因素Cox回归分析显示,均为预测SCLC患者铂类化疗OS的独立预后因素(P<0.05)。构建的列线图模型预测6、12、18个月OS的AUC值分别是训练组0.90(0.82~0.97),0.84(0.73~0.91),0.88(0.83~0.92);验证组0.80(0.66~0.94),0.82(0.69~0.96),0.86(0.72~0.95)。校准曲线显示,列线图预测概率与实际值之间具有较好的一致性,DCA结果表明模型的临床应用价值较高。训练组、验证组的Kaplan-Meier曲线均显示高风险组的患者与较短的总生存期相关(P<0.001)。结论:基于治疗前多维度参数构建的列线图可以为SCLC铂类化疗患者的OS预测提供一定的指导价值。

关键词：小细胞肺癌生物标记物铂类化疗列线图总生存期