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Dynamic tracking of stem cells in an acute liver failure model

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World Journal of Gastroenterology 2012年第6期18卷 507-516页

作者： Tarek Ezzat Dipok Kumar Dhar Massimo Malago Steven wm Olde Damink HPB and Liver Transplantation Surgery Royal Free HospitalUniversity College LondonLondon NW3 2PFUnited Kingdom Department of Surgery University of AlexandriaAlexandriaPO Box 21131Egypt Department of Surgery Maastricht University Medical Centre Nutrition and Toxicology Research Institute Maastricht UniversityMaastrichtPO Box 5800The Netherlands

AIM： To investigate a dual labeling technique, which would enable real-time monitoring of transplanted em- bryonic stem cell （ESC） kinetics, as well as long-term tracking. METHODS： Liver damage was induced in C57/BL6 male mice （n = 40） by acetaminophen （APAP） 300 mg/kg administered intraperitoneally. Green fluores- cence protein （GFP） positive C57/BL6 mouse ESCs were stained with the near-infrared fluorescent lipophilic tracer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbo- cyanine iodide （DiR） immediately before transplantationinto the spleen. Each of the animals in the cell therapy group （n = 20） received 5 x 106 ESCs 4 h following treatment with APAP. The control group （n = 20） re- ceived the vehicle only. The distribution and dynamics of the cells were monitored in real-time with the IVIS lumina-2 at 30 rain post transplantation, then at 3, 12, 24, 48 and 72 h, and after one and 2 wk. Immunohisto- chemical examination of liver tissue was used to identify expression of GFP and albumin. Plasma alanine amino- transferase （ALT） was measured as an indication of liver ***： DiR-stained ESCs were easily tracked with the IVIS using the indocyanine green filter due to its high background passband with minimal background autofluorescence. The transplanted cells were confined inside the spleen at 30 min post-transplantation, gradu- ally moved into the splenic vein, and were detectable in parts of the liver at the 3 h time-point. Within 24 h of transplantation, homing of almost 90% of cells was confirmed in the liver. On day three, however, the DiR signal started to fade out, and ex vivo IVIS imaging of different organs allowed signal detection at time-points when the signal could not be detected by in vivo imag- ing, and confirmed that the highest photon emission was in the liver （P 〈 0.0001）. At 2 wk, the DiRsignal was no longer detectable in vivo; however, immuno- histochemistry analysis of constitutively-expressed GFP was used to provide an insight into the d

关键词： Cell transplantation Cell tracking Embry-onic stem cells Acute liver failure Liver regeneration

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三种α径迹氡监测器的性能评价研究

国外医学（放射医学核医学分册） 1992年第5期16卷 205-208页

作者： Yeager wm 孟文斌

在37～740Bq·a·m^(-3)暴露量范围内,对三种α径迹氡监测器的性能进行了评价研究.给出了各自的精度和偏倚,讨论了研究结果和有关改进措施,选出了用于住宅氡调查的A型氡监测器.

关键词：氡监测器性能

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Fixation methods for electron microscopy of human and other liver

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World Journal of Gastroenterology 2010年第23期16卷 2851-2866页

作者： Eddie Wisse Filip Braet Hans Duimel Celien Vreuls Ger Koek Steven wm Olde Damink Maartje AJ van den Broek Bart De Geest Cees HC Dejong Chise Tateno Peter Frederik Electron Microscope Unit University of Maastricht Australian Key Center for Microscopy and Microanalysis Sydney University Department of Internal Medicine University of Maastricht Department of Surgery University of Maastricht Center for Molecular and Vascular Biology University of Leuven PhoenixBio Co.

For an electron microscopic study of the liver,expertise and complicated,time-consuming processing of hepatic tissues and cells is *** interpretation of electron microscopy(EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation,embedding,sectioning,contrast staining and microscopic ***,the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue,for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.

关键词： Liver Tissue fixation Perfusion Electron microscopy Biopsy

Comparison between empirical prokinetics, Helicobacter test-and-treat and empirical endoscopy in primary-care patients presenting with dyspepsia: A one-year study

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World Journal of Gastroenterology 2006年第31期12卷 5010-5016页

作者： Wayne HC Hu SK Lam Cindy LK Lam wm Wong KF Lam KC Lai YH Wong Benjamin CY Wong Annie OO Chan CK Chan Gabriel M Leung wm Hui Department of Medicine University of Hong Kong Hong Kong China Department of Statistics and Actuarial Science University of Hong Kong Hong Kong China Department of Community Medicine University of Hong Kong Hong Kong China

AIM： To investigate the optimal strategy to treat dyspeptic patients in primary care. METHODS： Dyspeptic patients presenting to primary care outpatient clinics were randomly assigned to- （1） empirical endoscopy, （2） H pylori test-and-treat, and （3） empirical prokinetic treatment with cisapride. Early endoscopy was arranged if patients remained symptomatic after 2 wk. Symptom severity, quality-of-life （SF-36） as well as patient preference and satisfaction were assessed. All patients underwent endoscopy by wk 6. Patients were followed up for one year. RESULTS： Two hundred and thirty four patients were recruited （163 female, mean age 49）. 46% were H pylori positive. 26% of H pylori tested and 25% of empirical prokinetic patients showed no improvement at wk 2 follow-up and needed early endoscopy. 15% of patients receiving empirical cisapride responded well to treatment but peptic ulcer was the final diagnosis. Symptom resolution and quality-of-life were similar among the groups. Costs for the three strategies were HK$4343, $1771 and $1750 per patient. 66% of the patients preferred to have early endoscopy. CONCLUSION： The three strategies are equally effective. Empirical prokinetic treatment was the least expensive but peptic ulcers may be missed with this treatment. The H pylori test-and-treat was the most cost-effective option .

关键词： Empirical endoscopy Dyspeptic patients Hpylori test-and treat

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管理决策法

管理决策法

作者： wm.E.·索德 wm.E.·Souder

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石油地质教育的回顾与展望

江汉石油学院学报 1994年第3期16卷 1-6页

作者： Ahr.,wm 邹华耀

阐述了世界石油工业与石油地质教育的历史及两者间相互促进的关系，指出了现今石油工业所面临的挑战。石油高校必须调整教学计划，培养多学科的综合性人材，以适应石油工业的发展。

关键词：石油工业石油地质学教育

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BLOOD CALCIUM IN SOUTHERN CHINESE

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中华医学杂志(英文版) 1935年第7期49卷 700-702页

作者： wm. W. CADBURY TING TSOK-YAN Canton Hospital Lingnan University Canton China.

In the course of an interesting paper on some special features of Medical practice in South China, Dr. Erich Pflomm (1) makes a rather startling statement. He declares that in his experience intravenous infusions of normal saline solution, so often a life saving device in European Clinics, is a highly dangerous and often even fatal measure when applied to Chinese patients.

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淋球菌与人中性白细胞间的相互作用

国外医学（微生物学分册） 1989年第6期12卷 265-268页

作者： Rest RF Shafer wm 牟杨龙

本文讨论了过去几年中完成的有关淋球菌与吞噬细胞间相互作用的实验,从细胞外活动、细胞表面活动和细胞内活动三方面阐述了淋球菌与吞噬细胞间相互作用的实验依据及可能的机理,指出了还存在的问题。

关键词：淋球菌吞噬细胞中性白细胞

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Principles of computer security : Security+ and beyond

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