Fixation methods for electron microscopy of human and other liver

作     者：Eddie Wisse Filip Braet Hans Duimel Celien Vreuls Ger Koek Steven WM Olde Damink Maartje AJ van den Broek Bart De Geest Cees HC Dejong Chise Tateno Peter Frederik 

作者机构：Electron Microscope UnitUniversity of Maastricht Australian Key Center for Microscopy and MicroanalysisSydney University Department of Internal MedicineUniversity of Maastricht Department of SurgeryUniversity of Maastricht Center for Molecular and Vascular BiologyUniversity of Leuven PhoenixBio Co. 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2010年第16卷第23期

页      面：2851-2866页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Liver Tissue fixation Perfusion Electron microscopy Biopsy 

摘      要：For an electron microscopic study of the liver,expertise and complicated,time-consuming processing of hepatic tissues and cells is *** interpretation of electron microscopy(EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation,embedding,sectioning,contrast staining and microscopic ***,the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue,for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.