检索结果-南通市图书馆

Chemical Research in Chinese Universities 2008年第5期24卷 584-587页

作者： HOU A-li meng qing-fan AN Jin-shuang ZHU Kai FENG Yun TENG Li-rong College of Life Science Jilin University Changchun 130012 P. R. China

The crude polysaccharide was obtained by means of the decolorization of porphyrized Cordyceps minlitaris stroma with organic solvent, extraction with hot water, precipitation in 80% ethanol, and protein removal with the Sevag method. After purification with Sephadex G-75, two of its components, CMP-1 and CMP-2, were obtained. Through the assay of gel chromatography and polarimetry, CMP-1 was identified as pure polysaccharide. The results demonstrated that CMP-1 had favorable oxidation resistance activity, which could scavenge not only oxygen-free radicals in the self-oxidation system of pyrogallic acid, but also the hydroxide-free radicals in the Fenton system. The study focused on the effects of low, medium, and high dosages of CMP-1 in rat blood serum on the proliferation of glomerular mesangial cells in vitro. Through MTT Colorimetric analysis, the activities were compared among the blank control group and the Niaoduqing positive control group CMP-1 and CMP-2. The results shows that CMP-1 was able to inhibit the proliferation of rat glomerular mesangial cells effectively. Therefore, CMP-1, one component of polysaccharides of Cordyceps minlitaris, was certainly a potential remedy for hyperplastic glomerular nephritis, whose antioxidant activity could slow down the process of chronic renal failure（CRF） to some extent.

关键词： Cordyceps minlitaris Polysaccharide Glomerular mesangial cell Oxidation resistance Chronic renal failure

Purification and in vitro Activity of an Antimicrobial Peptide from Skin of Rana Temporaria Chensinensis,David

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2007年第4期23卷 433-436页

作者： ZHOU Jie meng qing-fan XU Xue-song TIAN Xiao-le JIANG Fu-jia LI qing-shan TENG Li-rong College of Life Science Jilin University Changchun 130012 P. R. China Department of Laboratory China-Japan Union Hospital Jilin University Changchun 130033 P. R.China

In this study, an antimicrobial component（RTCI） was purified from the skin of Rana temporaria chensinensis, David. Antimicrobial activities of RTCI against clinical multi-drug resistant bacterial strains, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureaus, Klebsiella oxytoca, Klebsiella pneumoniae, Enterococcus fae-calis, and Proteus mirabilis, were measured in vitro by means of minimal inhibitory concentration and time-kill studies. The results indicate that RTCI could inhibit the growth of these bacteria at a proper concentration and suggest that RT-CI shows a better antimicrobial activity to Gram-negative bacterial strains than to Gram-positive bacterial strains.

关键词： Antimicrobial peptides Multi-drug resistant Amphibian skin

Protein Adsorption onto Nanosized Hydroxyapatite Particles for Controlled Drug Release

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2007年第3期23卷 254-257页

作者： WANG Bo TENG Li-rong WANG Chun-yan meng qing-fan ZHAO Ling-zhi GAO Bo College of Life Science Jilin University Changchun 130012 P. R. China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry Jilin University Changchun 130012 P. R. China

Nanosized hydroxyapatite（nsHAp） was synthesized to examine its possibility as a controlled release cartier of protein. To achieve effective protein release from nanosized hydroxyapatite, the study of the adsorptive properties of protein on nsHAp and different influence parameters such as pH, calcium, and phosphate concentrations during the adsorption process is necessary. Ovalbumin（OVA） was selected as the model of growth factors. The results show that the amount of OVA adsorbed onto nsHAp in acetic buffer（pH = 3.6） is more than that in acetic buffer（pH =5.6） because of the electric interaction. The amount of OVA adsorption in phosphate buffer solution（PBS） is smaller than that in acetic buffer because of surface complexation and surface hydroxylation. The presence of Ca^2＋ dramatically increases the adsorbed amount of OVA in acetic buffer on maintaining the same pH. Meanwhile, the release kinetics of OVA adsorbed onto nsHAp（nsHAp-OVA） was also examined. The amount of released OVA in PBS（pH = 5.6） was significantly smaller than that released in solution of pH = 7.0. All the results suggest that nanosized hydroxyapatite particles could be successfully used as controlled released carrier of protein.

关键词： Nanomaterials Hydroxyapatite Ovalbumin Controlled release

Purification and Properties of Superoxide Dismutase(SOD) in Allium Sativum

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2003年第4期19卷 442-445页

作者： TENG Li-rong WANG Ya-jun WU Min HONG Shui-sheng CHEN Jia meng qing-fan LIU Lan-ying College of Life Science Jilin UniversityChangchun 130023P.R.China

Superoxide dismutases(SODs) were purified to homogeneity from Allium Sativum by means of ammonium sulfate precipitation and column chromatography with DEAE-cellulose(DE52) and Sephadex G-75. Based on sodium dodecyl sulfate\|polyacrylamide gel electrophoresis(SDS-AGE), Allium Sativum is predicted to contain four SODs. The molecular weights of the native SODs are 41 3 kD, 37 0 kD, 35 2 kD and 31 0 kD, which consist of subunits of 20 7 kD, 18 4 kD, 17 7 kD and 15 4 kD respectively. Because of their specific sensitivity to hydrogen peroxide, cyanogens potassium and chloroform\|alcohol, the SODs in Allium Sativum appear to be Cu, Zn-SOD isoenzymes. The isoelectric analysis indicates that three of the four isoenzymes are acidic proteins with isoelectric points at pH 3 5, 3 7 and 4 0, respectively, and the fourth one is a basic protein with isoeletric point at pH 8 5.

关键词： Superoxide dismutase Allium Sativum Purification Properties

Isolation,Purification and Bioactivities of Polysaccharides from Irpex lacteus

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2012年第2期28卷 249-254页

作者： ZHANG Na LIU Yan LUJia-hui WANG Juan YANG Shuang ZHANG Nan meng qing-fan TENG Li-rong College of Life Science Jilin UniversityChangchun 130012P.R.China Department of Chemistry and Pharmacy Zhuhai College of Jilin UniversityZhuhai 519041P.R.China

Irpex lacteus has been widely used for treating chronic glomerulonephritis as a traditional Chinese *** water-soluble polysaccharide fractions（ILN I,ILN II,ILN III,ILA I,ILA II,ILB I and ILB II） were isolated and purified from Irpex lacteus by hot-water extraction,deproteinization,decolorization,dicthy laminoethyl（DEAE）-cellulose ion exchange and sephadex G100 chromatographies,*** average molecular weights and monosaccharide composition of these polysaccharide fractions greatly differed from each *** antitumor and antinephritis activities of the seven polysaccharide fractions were *** was found that ILN III displayed significant inhibition effects on both humar hepatocellular liver carcinoma（HepG2） and hentietta lacks（HeLa） tumor cells with IC 50 values of 60.95 and 99.95 μg/mL,*** I exhibited significant inhibition effects on murine mesangial cells（HBZT-1） with an IC 50 value of 185.06 μg/*** inhibition effects of other polysaccharide fractions on these three cells were significantly *** results suggest that the polysaccharide fractions isolated from Irpex lacteus have potential antitumor and antinephritis activities.

关键词： Irpex lacteus Polysaccharide Purification Bioactivity

Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Method

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2008年第4期24卷 487-490页

作者： GAO Chao-hui SHI Xiao-yue HOU Xin-tong meng qing-fan Zhang Ying-jiu TENG Li-rong College of Life Science Jilin University Changehun 130012 P. R. China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changehun 130021 P. R. China Changehun Diseases Control and Prevention Center Changehun 130033 P. R. China College of Animal Science and Veterinary Medicine Jilin University Changehun 130062 P. R. China

According to the amino acid sequence and codon preference of E. coli, the human interleukin-18（IL-18） gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides, DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method. The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector. Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. for sequencing. The sequencing result shows that one clone contained the complete correct gene in all the five positive clones.

关键词： Human IL-18 Gene synthesis One-step method Oligodeoxynucleotide synthesis

First-order Derivative Spectrophotometry for the Determination of Vitamin C in Medicament

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2008年第1期24卷 29-31页

作者： meng qing-fan TENG Le-sheng JIANG Chao-jun An Jin-shuang LI Lei LU Jia-hui TENG Li-rong College of Life Science Jilin University Changchun 130012 P. R. China

A novel method for the determination of vitamin C（Vc） is proposed in this article. After the reaction with Folin-Ciocalteau reagent at ambient temperature, Vc solution was scanned at 750--1100 nm, and its first-order derivative spectrum were obtained from the original spectrum. The values of derivative selected at 995 nm were used for determination. It was proved that Vc could quickly react with Folin-Ciocalteau reagent within 5 min and the product was quite stable for a long time. The conditions required for this method is not very complicated, its precision and accuracy are similar to those of the iodometric titration described in Chinese Pharmacopoeia, and the limit of detection is 0.312 μg/mL. The determination of the results of vitamin C tablet, pill, and injection demonstrates that this method has wide pharmaceutical applications.

关键词： First-order derivative spectroscopy Vitamin C determination Folin-Ciocalteau regent

Forced Degradation of Flavonol Glycosides Extraced from Ginkgo biloba

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2013年第4期29卷 667-670页

作者： JIN Ye ZHANG Wei-yu meng qing-fan LI Dan-hui GARG Sajay TENG Li-rong WEN Jlng-yuan College of Life Science Jilin University Changchun 130012 P. R. China School of Pharmacy Faculty of Medical and Health Sciences University of Auckland 1010 Auckland New Zealand School of Pharmacy Changchun University of Chinese Medicine Changchun 130117 P R. China School of Pharmacy and Medical Sciences University of South Australia Adelaide South Australia 5001. Australia

The degradation of flavonol glycosides extracted from Ginkgo biloba was performed under different conditions and the degraded products were determined by reversed-phase high performance liquid chromatography （RP-HPLC） *** stress conditions including acid（0.1 mol/L HCl）,base（0.1 mol/L NaOH）,temperature （70 ℃） and oxidation（0.03% H2O2,volume fraction） were used for the forced degradation *** pH stabilities of the flavonol glycosides were determined in phosphate buffers of varying pH values from 4.5 to *** degradation rate constants and half-life of three Ginkgo flavonol aglycones（quercetin,kaempferol and isorhamnetin） which represent Ginkgo flavonol glycosides were calculated in forced degradation and pH-stability studies of *** resuits indicate that the three substances were more stable when incubated under acid condition and showed pH-dependent *** degradation was observed to follow first-order kinetics in all degradation *** stability results could provide important bases on development,preparation and storage of products of Ginkgo biloba extract and should be significantly considered during the further formulation development.

关键词： Ginkgo flavonol glycoside Degradation pH-Stability Degradation rate constant

Preparation of Tolterodine Metabolite Loaded Biodegradable PLGA Microspheres

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2010年第1期26卷 75-80页

作者： TENG Le-sheng JIANG Chao-jun LIN Xiang-you DONG Yuan-chen LI Chun-mei meng qing-fan LI You-xin College of Life Science Jilin University Changehun 130012 P. R. China

Biodegradable polymer poly（lactic-co-glycolic acid）（PLGA） was used to encapsulate the pharmacological activity metabolite of tolterodine by means of O/W emulsion solvent evaporation method via homogenization in the emulsification process. The influences of preparation parameters were investigated. The results indicate that increa- sing PLGA concentration from 15% to 40% made the encapsulation efficiency of 5-hydroxymethyl derivative of tol- terodine（5-HMT） increased from 55.39% to 76.32%, and the particle size increased from 34.33 μm to 70,78 lain. In addition, when homogenization speed increased from 850 r/min to 2300 r/min, both particle size and encapsulation efficiency of microspheres decreased. An increase in the volume of aqueous phase led to higher encapsulation efficiency and bigger particle size. Increasing temperature made encapsulation efficiency and particle size change significantly. While reaction temperature increased from 20 ℃ to 50 ℃, the encapsulation efficiency decreased from 70.44% to 24.07%, and particle size increased from 38.66 μm to 69.38 μm. High reaction temperature（over 40 ℃） may lead to porous surface of microspheres. Porous surface, encapsulation efficiency and particle size influenced on the in vitro release of 5-HMT together.

关键词： Poly(lactic-co-glycolic acid) Microsphere Tolterodine Tolterodine metabolite

Application of Near Infrared Diffuse Reflectance Spectroscopy with Radial Basis Function Neural Network to Determination of Rifampincin Isoniazid and Pyrazinamide Tablets

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2007年第5期23卷 518-523页

作者： DU Lin-na WU Li-hang LU Jia-hui GUO Wei-liang meng qing-fan JIANG Chao-jun SHEN Si-le TENG Li-rong College of Life Science College of Animal Science and Veterinary Medicine College of Chemistry Jilin University Changchun 130012 P. R. China

Partial least squares（PLS）,back-propagation neural network（BPNN）and radial basis function neural network（RBFNN）were respectively used for estalishing quantative analysis models with near infrared（NIR）diffuse reflectance spectra for determining the contents of rifampincin（RMP）,isoniazid（INH）and pyrazinamide（PZA）in rifampicin isoniazid and pyrazinamide ***-Golay smoothing,first derivative,second derivative,fast Fourier transform（FFT）and standard normal variate（SNV）transformation methods were applied to pretreating raw NIR diffuse reflectance *** raw and pretreated spectra were divided into several regions,depending on the average spectrum and RSD *** component analysis（PCA）method was used for analyzing the raw and pretreated spectra in different regions in order to reduce the dimensions of input *** optimum spectral regions and the models＇ parameters were chosen by comparing the root mean square error of cross-validation（RMSECV）values which were obtained by leave-one-out cross-validation *** RMSECV values of the RBFNN models for determining the contents of RMP,INH and PZA were 0.00288,0.00226 and 0.00341,*** these models for predicting the contents of INH,RMP and PZA in prediction set,the RMSEP values were 0.00266,0.00227 and 0.00411,*** results are better than those obtained from PLS models and BPNN *** additional advantages of fast calculation speed and less dependence on the initial conditions,RBFNN is a suitable tool to model complex systems.

关键词： Rifampicin isoniazid and pyrazinamide tablets NIR diffuse reflectance spectroscopy Partial least square Back-propagation neural network Radial basis function neural network