Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Method

作     者：GAO Chao-hui SHI Xiao-yue HOU Xin-tong MENG Qing-fan Zhang Ying-jiu TENG Li-rong 

作者机构：College of Life Science Jilin University Changehun 130012 P. R. China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changehun 130021 P. R. China Changehun Diseases Control and Prevention Center Changehun 130033 P. R. China College of Animal Science and Veterinary Medicine Jilin University Changehun 130062 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2008年第24卷第4期

页      面：487-490页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0703[理学-化学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：Human IL-18 Gene synthesis One-step method Oligodeoxynucleotide synthesis 

摘      要：According to the amino acid sequence and codon preference of E. coli, the human interleukin-18（IL-18） gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides, DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method. The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector. Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. for sequencing. The sequencing result shows that one clone contained the complete correct gene in all the five positive clones.